Biology Commons^™

Development Of A Biomarker Panel For Identifying Stressed Marine Mammals, Laura Pujade

University of the Pacific Theses and Dissertations

Increasing anthropogenic disturbance in marine ecosystems such as fishing, oil-drilling, and noise pollution can have detrimental effects on the reproduction and survival of apex predators such as marine mammals. Stress activates the hypothalamic-pituitary-adrenal (HPA) axis, resulting in increased circulating glucocorticoid (GCs) hormones, which alter expression of target genes encoding metabolic enzymes and other mediators of stress. Prolonged HPA axis stimulation may increase catabolism of nutrient stores and suppress immune and reproductive functions, impacting the fitness of marine mammals. GCs measurements are used to identify wild animals experiencing stress. However, these measurements may not be sensitive enough to distinguish between an …

Mapping Status And Conservation Of Global At-Risk Marine Biodiversity, Casey C. O'Hara, Juan Carlos Villaseñor-Derbez, Gina M. Ralph, Benjamin S. Halpern

Biological Sciences Faculty Publications

To conserve marine biodiversity, we must first understand the spatial distribution and status of at‐risk biodiversity. We combined range maps and conservation status for 5,291 marine species to map the global distribution of extinction risk of marine biodiversity. We find that for 83% of the ocean, >25% of assessed species are considered threatened, and 15% of the ocean shows >50% of assessed species threatened when weighting for range‐limited species. By comparing mean extinction risk of marine biodiversity to no‐take marine reserve placement, we identify regions where reserves preferentially afford proactive protection (i.e., preserving low‐risk areas) or reactive protection (i.e., mitigating …

Parasitic Dinoflagellate Hematodinium Perezi Prevalence In Larval And Juvenile Blue Crabs Callinectes Sapidus From Coastal Bays Of Virginia, H. J. Small, J. P. Huchin-Mian, K. S. Reece, K. M. Pagenkopp Lohan, Mark J. Butler Iv, J. D. Shields

Biological Sciences Faculty Publications

The parasitic dinoflagellate Hematodinium perezi infects the American blue crab Callinectes sapidus and other decapods along the Eastern seaboard and Gulf of Mexico coast of the USA. Large juvenile and adult blue crabs experience high mortality during seasonal outbreaks of H. perezi, but less is known about its presence in the early life history stages of this host. We determined the prevalence of H. perezi in megalopae and early benthic juvenile crabs from multiple locations along the Virginia portion of the Delmarva Peninsula. The DNA of H. perezi was not detected in any megalopae collected from several locations within …

Seasonal Spatial Segregation In Blue Sharks (Prionace Glauca) By Sex And Size Class In The Northeast Pacific Ocean, Sara M. Maxwell, Kylie L. Scales, Steven Bograd, Dana K. Briscoe, Heidi Dewar, Elliott L. Hazen, Rebecca L. Lewison, Heather Welch, Larry B. Crowder

Biological Sciences Faculty Publications

Aim: Animal tracking can provide unique insights into the ecology and conservation of marine species, such as the partitioning of habitat, including differences between life history stages or sexes, and can inform fisheries stock assessments, bycatch reduction and spatial management such as dynamic management.

Location: Northeast Pacific Ocean.

Methods: We used satellite tracking data from 47 blue sharks (Prionace glauca) from the Northeast Pacific to determine movements and home range along the west coast of North America, and sex–size class (immature females, mature males) specific habitat preferences using boosted regression trees. Using a suite of static and dynamic …

Effects Of Aquatic Acidification On Calcium Uptake In White River Shrimp Litopenaeus Setiferus Gills, Maria-Flora Jacobs

UNF Graduate Theses and Dissertations

Previous research regarding aquatic acidification has examined the protonation of the carbonate and does not consider calcium to be a limiting factor. This is the first study to suggest that pH may affect the uptake of calcium in crustacean gills. This project describes ion transport mechanisms present in the cell membranes of white river shrimp Litopenaeus setiferus gill epithelium, and the effects of pH on the uptake of calcium by these means. Partially purified membrane vesicles (PPMV) of shrimp gills were prepared through a homogenization process that has been used previously to define ion transport in crab and lobster gill …

Spatial Variability In Recruitment Of Chilipepper Rockfish (Sebastes Goodei) In The California Current System, Laura K. Solinger

Cal Poly Humboldt theses and projects

Properly describing variability in population dynamics (e.g., growth, fecundity, recruitment) is expected to improve management of important fisheries stocks (Maunder & Piner, 2014). As recruitment to most fish stocks is determined during early life history stages (Houde, 1997; Iles & Beverton, 2000), and early life history stages are influenced by oceanographic conditions (Bjorkstedt et al. 2002; Laidig, 2010), understanding how environmental stochasticity influences recruitment deviations has potential to improve estimates of stock productivity and how productivity might change over time to support more effective management. Chilipepper Rockfish (Sebastes goodei) are an important commercial species that is managed as …

Role Of Managed Marine Areas On The Diversity And Individual Responses Of Rocky Intertidal Shore Grazers In Central Chile, Kathy Liu

Scripps Senior Theses

Many different types of marine benthic herbivores or “grazers” inhabit coastal intertidal zones and play a crucial role in inter- and shallow subtidal ecosystems. Chile has one of the most diverse intertidal zones, but many intertidal grazers are exploited for human consumption. Marine protected areas (MPAs) and marine management and exploitation areas (MEAs) are promising tools for Chile to combat over exploitation of these grazer and other marine resources. This study surveyed the impact of sites with contrasting management on the diversity and abundance of all intertidal grazers and their impact on the size frequency and shell length-body weight allometry …

Living Shoreline Stabilization To Increase Resiliency At De Soto National Memorial, Linda Walters, Melinda Donnelly, Christian Pilato, Suzanne Connor

CEELAB Research Data

De Soto National Memorial (Bradenton, FL) is an important location where both Native Americans and De Soto's army of soldiers once lived. We have been working closely with the National Park Service and the University of Central Florida to understand how to best stabilize this shoreline using living shoreline techniques combined with long-term natural recruitment. Stabilizing De Soto's shoreline is essential to decrease erosion and shoreline loss to protect the Park's cultural and natural resources, especially the numerous historically significant shell middens. Through pre-restoration monitoring, we determined that high-energy boat wakes were limiting natural mangrove recruitment. Lack of retention of …

Legacy Habitat Suitability Of Eastern Oysters (Crassostrea Virginica) In Louisiana: A Prelude To Mississippi River Delta Freshwater Diversions, Tasia Mv Denapolis

LSU New Orleans Theses and Dissertations

The Louisiana oyster industry is greatly impacted by freshwater and sediment diversions that are part of the effort to restore the state’s coastline. A habitat suitability index (HSI) proposes species-habitat relationships that can be instrumental in creating impact assessments and suitability predictions for management as new diversions are implemented. An oyster (Crassostrea virginica) HSI was developed using three variables crucial to oyster sustainability: average annual salinity, minimum monthly salinity, and average salinity during the spawning season. These Legacy HSI visualizations show annual fluctuations in the distribution of zones suitable for oyster cultivation prior to proposed diversions in Pontchartrain …

High-Frequency Temperature Variability Mirrors Fixed Differences In Thermal Limits Of The Massive Coral Porites Lobata, Daniel J. Barshis, Charles Birkeland, Robert J. Toonen, Ruth D. Gates, Jonathon H. Stillman

Biological Sciences Faculty Publications

Spatial heterogeneity in environmental characteristics can drive adaptive differentiation when contrasting environments exert divergent selection pressures. This environmental and genetic heterogeneity can substantially influence population and community resilience to disturbance events. Here, we investigated corals from the highly variable back-reef habitats of Ofu Island in American Samoa that thrive in thermal conditions known to elicit widespread bleaching and mortality elsewhere. To investigate the relative importance of acclimation versus site of origin in shaping previously observed differences in coral tolerance limits at Ofu Island, specimens of the common Indo-Pacific coral Porites lobata from locations with differing levels of thermal variability were …

Shoreline Characterization In The Northern Indian River Lagoon, Melinda Donnelly, Michelle Shaffer, Suzanne Connor, Linda Walters

CEELAB Research Data

The purpose of this project was to evaluate current conditions of estuarine shorelines in the northern section of the Indian River Lagoon system, including Mosquito Lagoon, north Indian River, and Banana River. From January 2016 through June 2018, we evaluated structural and functional characteristics of 374 miles of shoreline, from Ponce Inlet in Volusia County to Sebastian Inlet in Brevard County (11,000+ data points). Hard-armoring accounted for approximately 50% of total shoreline, evenly divided between shoreline with bulkheads (25%) and hardened slopes (25%). Forty-five percent of shoreline without hard-armoring had anthropogenic alterations (i.e. mosquito impoundments, railroads and roadways, residential and …

Mitochondrial Dna Sequence Alignments For Phylogenetic Analysis Of The Shrimp Genus Farfantepenaeus, Laura Timm, S. Simon, Heather Bracken-Grissom Dr.

DEEPEND Datasets

This dataset includes 12S, 16S, and COI sequence data. Sequences were obtained from the eight recognized species of Farfantepenaeus: F. aztecus, F. brasiliensis, F. brevirostris, F. californiensis, F. duorarum, F. isabelae, F. notialis, and F. subtilis. Also included are several sequences from species of Litopenaeus, which serve as outgroup(s).

Mortality Rate Trends Of Australian Fur Seal Pups, Arctocephalus Pusillus Doriferus, And Possible Factors Affecting Pup Mortality In The Bass Strait Of Australia, Theresa Waters

Independent Study Project (ISP) Collection

Australian fur seals (AFS), Arctocephalus pusillus doriferus, are upper trophic predators endemic to the Bass Strait of south-eastern Australia. Their populations have been monitored since the mid-1900s to assess ecosystem health and the continual recovery of the species from extensive hunting in the early 19th century. The best way to track the environmental conditions and recovery of AFS populations is by monitoring the pups that are confined to the breeding colonies. This study looked particularly at the mortality rates of AFS pups because mortality of the young is a good indicator of population dynamics. The aim of this study was …

What Can Computational Modeling Tell Us About The Diversity Of Odor-Capture Structures In The Pancrustacea?, Lindsay D. Waldrop, Yanyan He, Shilpa Khatri

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

A major transition in the history of the Pancrustacea was the invasion of several lineages of these animals onto land. We investigated the functional performance of odor-capture organs, antennae with olfactory sensilla arrays, through the use of a computational model of advection and diffusion of odorants to olfactory sensilla while varying three parameters thought to be important to odor capture (Reynolds number, gap-width-to-sensillum-diameter ratio, and angle of the sensilla array with respect to oncoming flow). We also performed a sensitivity analysis on these parameters using uncertainty quantification to analyze their relative contributions to odor-capture performance. The results of this analysis …

The Transcriptome Of The Bermuda Fireworm Odontosyllis Enopla (Annelida: Syllidae): A Unique Luciferase Gene Family And Putative Epitoky-Related Genes, Mercer R. Brugler, M. Teresa Aguado, Michael Tessler, Mark Siddall

Publications and Research

The Bermuda fireworm Odontosyllis enopla exhibits an extremely tight circalunar circadian behavior that results in an impressive bioluminescent mating swarm, thought to be due to a conventional luciferase-mediated oxidation of a light-emitting luciferin. In addition, the four eyes become hypertrophied and heavily pigmented, and the nephridial system is modified to store and release gametes and associated secretions. In an effort to elucidate transcripts related to bioluminescence, circadian or circalunar periodicity, as well as epitoky-related changes of the eyes and nephridial system, we examined the transcriptomic profile of three female O. enopladuring a bioluminescent swarm in Ferry Reach, Bermuda. …

Bubble Stream Production By Belugas (Delphinapterus Leucas), Megan Slack

Theses

Bubble stream production in belugas has been poorly characterized and its function is not well understood. I examined behavioral states when producing bubble streams (“bubbling”), and when bubbling calls, to determine whether bubbling was significantly associated with a particular call category or behavioral state. Using 19 hours of video and audio recordings collected over a two-day period, I quantified bubble streams of a 4-month old calf and an unrelated adult female housed together. Based on the overall activity budgets and pool of vocalizations for both animals, I calculated the expected counts of bubble streams with and without vocalizations, assuming that …

Spatial Distribution And Stock-Recruitment Analysis Of The Atlantic Surfclam, Spisula Solidissima, In The Mid-Atlantic Bight And On Georges Bank, Jeremy Timbs

Master's Theses

Atlantic surfclams, Spisula solidissima, are long-lived bivalve molluscs with dispersive larval stages. Surfclam patchiness throughout the U.S. Exclusive Economic Zone (EEZ) has declined from the 1980’s to the present in all assessed regions (Delmarva, New Jersey, Long Island, Southern New England, and Georges Banks). Warming of Mid-Atlantic bottom waters is driving the surfclam stock into new habitat and extirpating the surfclams from nearshore areas. Based on a species distribution function model, the small surfclams appear to inhabit a greater area than the large market-size surfclams across the entire stock. The wider distribution of recent recruits relative to the fishable …

Effects Of Drift Macroalgae On Sediment Nitrogen Cycling In Thalassia Testudinum Beds Of St. Joseph Bay, Fl, Erica Strope

Master of Science in Integrative Biology Theses

Anthropogenic nitrogen (N) loading and eutrophication can affect valuable ecosystem services and seagrass habitats by modifying structural and functional aspects of estuarine communities including increasing and prolonging macroalgae blooms. In some contexts, macroalgae may play a key role in N cycling pathways because they can alter sediment chemistry. Previous research has associated drift macroalgae blooms with elevated dissolved inorganic N concentrations in sediments as a result of increased remineralization of organic matter, but drift macroalgae effects on microbial N transformation pathways are not well understood. This study quantified the effects of macroalgae on estuarine N cycling in Thalassia testudinum seagrass …

Successional Processes In The Benthic Invertebrate Communities At Gray’S Reef National Marine Sanctuary, Alexis A. Bivens

Honors College Theses

While the process of community development has been studied in terrestrial habitats since the turn of the 20th century, similar information is not as readily available in marine systems. Understanding patterns of community development is essential to predicting recovery potential and to designing effective marine protected areas. In the South Atlantic Bight, invertebrate communities on hard substrata can differ significantly from one rocky outcrop to another, but the factors driving these differences are not well understood. I documented the initial development of the benthic invertebrate community at Gray’s Reef National Marine Sanctuary (GRNMS) to address the prediction that this system …

Presence/Absence And Density Data For Epipelagic Tows Collected During R/V Blazing Seven Cruises Lf2016a And Lf2016b, Northern Gulf Of Mexico From 2016-06-09 To 2016-07-28, Jay R. Rooker, David Wells

DEEPEND Datasets

This dataset reports presence/absence and density data for epipelagic tows collected in the northern Gulf of Mexico during R/V Blazing Seven cruises LF2016A and LF2016B (2016-06-09 to 2016-07-28). Larval fishes were sampled from 48 stations and cruise data were collected at each site including latitude/longitude, date, time, environmental data (temperature, salinity, dissolved oxygen) and Sargassum dry weight. Larval catch data before and after the oil spill will be compared to improve our understanding of the causes of temporal variability as it relates to the Deep-Water Horizon oil spill (DWHOS). Habitat associations of selected taxa (billfishes, tunas, dolphinfishes, flyingfishes) will be …

Inventory Of Oceanic Fauna Data Including Species, Weight, And Measurements From R/V Point Sur (Cruise Dp05) In The Gulf Of Mexico From 2017-05-01 To 2017-05-11, April Cook, Tracey Sutton

DEEPEND Datasets

This dataset includes an inventory of Gulf of Mexico oceanic fauna data including species, weight, and measurements collected from R/V Point Sur (Cruise DP05) from 2017-05-01 to 2017-05-11. The main gear type used was a 10-m2 Multiple Opening Closing Net and Environmental Sensing System (MOCNESS). The MOCNESS was fitted with 6 nets which were opened according to the following depth scheme: net 0 from the surface to 1500m, net 1 from 1500-1200m, net 2 from 200-1000m, net 3 from 1000-600m, net 4 from 600-200m, and net 5 from 200m to the surface. Two trawls were conducted at each station sampled …

Foraging Ecology Of Parrotfishes In The Greater Caribbean: Impacts Of Specialization And Dietary Preferences On Marine Benthic Communities, Madelyn V. Roycroft

Master's Theses

Coral reefs are one of the world’s most diverse yet heavily impacted marine ecosystems. As a result of many direct and indirect stressors, coral reefs have experienced major degradation over the last several decades. Declines in coral reefs in the Caribbean have been particularly acute and generally associated with the loss of key herbivores and an increase in algae. Herbivorous fishes such as parrotfishes can positively impact coral reefs by removing algae that compete with corals for light and space. However, many parrotfishes are also important coral predators. Predation on corals, known as corallivory, can adversely affect coral growth, reproduction …

Effect Of Adult Chemical Cues On Molting Of Fiddler Crab Megalopae In Low Salinity Seawater, Sydney Rilum

Undergraduate Honors Theses

Three species of fiddler crabs, Uca minax, U. pugnax, and U. pugilator, are commonly found in estuaries along the Atlantic coast, each with distinct adult habitats differing in salinity and sediment grain size. Prior research has found evidence for larvae exhibiting selective settlement; however, the degree to which and the method by which they choose their species-appropriate habitat to settle in is still unknown. Additionally, a recent study determined that chemical cues from adult crabs stimulate molting in field-caught fiddler crab megalopae, as previously determined in lab-reared megalopae; however, in 35 ppt seawater, few U. minax molted. …

Waking Students Up With Explorations Of Metabolic Dormancy: A Pilot Study Of The Killifish Cure, Emma Goodwin, Daniel E. Zajic, Jason E. Podrabsky, Erin E. Shortlidge

Student Research Symposium

A national goal for education reform is to provide opportunities for all biology undergraduates to participate in research. Traditionally, undergraduates gain research experience by apprenticing in faculty-led research labs; however, only a fraction of undergraduates have access to these opportunities. Course-based Undergraduate Research Experiences (CUREs) are a type of laboratory course where students conduct experiments to answer relevant research questions, thus allowing undergraduates to gain authentic research experience at a large scale. To increase equity in opportunities for biology undergraduates to participate in authentic scientific research at Portland State University (PSU), we have designed and implemented a CURE to pilot …

Preliminary Work On Infestation Dynamics And Stable Isotope Ecology Of A Coral Reef Micropredator, William Garrett Jenkins

Student Theses and Dissertations

Despite the significant ecological role that parasites likely play within their respective communities, little is understood about the role that they play within food webs. The studies presented herein sought to expand upon the understanding of the role that gnathiid isopods, a marine fish ectoparasite, play within coral reef ecological communities by: a) assessing the effect of host physical condition on susceptibility to exploitation by a gnathiid isopod and b) by conducting a comprehensive, preliminary, study on the stable isotope ecology of these micropredators. Host fish which were experimentally injured during field trials exhibited a higher probability of being parasitized …

Evaluating In Situ Grazing Patterns Of Lytechinus Variegatus And Their Effects On Seagrass Beds Of Thalassia Testudinum, Adrianna Parson

Master of Science in Integrative Biology Theses

The sea urchin Lytechinus variegatus is a known grazer of the seagrass Thalassia testudinum, and has been known to denude vast stands of seagrass beds at high densities. Outside of these denudation events, the effects of sea urchins on seagrass are poorly understood. This study examines the effects of L. variegatus on T. testudinum in situ, to understand how sea urchins are affecting seagrasses in situ. Results indicate that urchins were found in the offshore portion of the seagrass bed at densities up to 4 urchins/m². Changes in temperature and sediment size in the bay indicate …

Changes In The Microbial Community Of Lubomirskia Baicalensis Affected By Red Sponge Disease, Colin Rorex

Electronic Theses and Dissertations

Lake Baikal is the oldest known lake and a unique ecosystem, home to several species of fresh water sponge. A disease outbreak affecting the dominant species, Lubormirskia baialensis, was recently reported. The cause of the disease has not been determined but one of the current hypothesis is that the increase in methane concentration is correlated to the disease outbreak. This pilot study characterized the microbiomes of sick and healthy sponges through the use of 16S rRNA sequencing. Sick sponge microbiomes shared a conserved group of taxa while the healthy sponge microbiomes had greater diversity. Indicator species analysis identified two significant …

Correlation Of Environmental Exposure To Polycyclic Aromatic Hydrocarbons And Polymorphisms In The Proto-Oncogene V-Rel Using Wild Atlantic Menhaden, Chelsea Rose Defelice

Seton Hall University Dissertations and Theses (ETDs)

Hurricane Sandy critically damaged the Atlantic coast of New Jersey in the fall of 2012. This was recorded as the largest storm to hit the Atlantic Ocean, which was manifested by its destruction on the homes and communities of those along the coastline. Contamination of surrounding waters due to this natural disaster was of great interest. The re-suspension of land-based pollution is proposed to make these contaminants more bioavailable to coastal species, such as Atlantic menhaden (Brevoortia tyrannus), which are a crucial biomonitoring species through their ecological and economic uses. A proposed increase of body burdens in Atlantic …

A Phylogeographic Analysis Of Black Coral (Antipatharia) On Artificial And Natural Reefs In The Northwestern Gulf Of Mexico, Heather Otte

Theses and Dissertations

NOAA's Islands in the Stream concept (IitS) of designating networks of marine protected areas as refugia in the Gulf of Mexico depends on genetic connectivity. Isolated habitats like those proposed by the IitS occur naturally off the Texas coast where hard-bottom structures protrude from mudflats, offering a small-scale study of the IitS feasibility. Two sequences, mitochondrial 16S ribosomal RNA and nuclear internal transcribed spacer, were used to build haplotype networks of Antipatharians (Anthozoa: Hexacorallia), corals with planktonic larvae that can act as a proxy for connectivity of other organisms. Two species showed no significant genetic differentiation or pattern of genetic …

Emptying And Refilling Of Slime Glands In Atlantic (Myxine Glutinosa) And Pacific (Eptatretus Stoutii) Hagfishes, Sarah Schorno, Todd E. Gillis, Douglas S. Fudge

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Hagfishes are known for their unique defensive slime, which they use toward off gill-breathing predators. Although much is known about the slime cells (gland thread cells and gland mucous cells), little is known about how long slime gland refilling takes, or how slime composition changes with refilling or repeated stimulation of the same gland. Slime glands can be individually electrostimulated to release slime, and this technique was used to measure slime gland refilling times for Atlantic and Pacific hagfish. The amount of exudate produced, the composition of the exudate and the morphometrics of slime cells were analyzed during refilling, and …