Biology Commons^™

3 Genes Of The Map Kinase Cascade, Mek-2, Mpk-1/Sur-1 And Let-60 Ras, Are Required For Meiotic Cell-Cycle Progression In Caenorhabditis-Elegans, Diane L. Church, Kun Liang Guan, Eric J. Lambie

Dartmouth Scholarship

In the germline of Caenorhabditis elegans hermaphrodites, meiotic cell cycle progression occurs in spatially restricted regions. Immediately after leaving the distal mitotic region, germ cells enter meiosis and thereafter remain in the pachytene stage of first meiotic prophase for an extended period. At the dorsoventral gonadal flexure, germ cells exit pachytene and subsequently become arrested in diakinesis. We have found that exit from pachytene is dependent on the function of three members of the MAP kinase signaling cascade. One of these genes, mek-2, is a newly identified C. elegans MEK (MAP kinase kinase). The other two genes, mpk-1/sur-1 (MAP kinase) …

The Bag And Size Limit Review: New Regulations And Summary Of Submissions, F. B. Prokop, J M. Collins

Fisheries Management Papers

Since the new bag and size limits were introduced in December 1991, there have been many suggestions for refinement and improvement of these regulations. This paper includes the summary of submissions to Fisheries Management Paper No 52 - Review of Bag and Size Limit Proposals for Western Australian Recreational Fishers and details new regulations which will come into force on 1 July 1995.

The Best Available Information - Its Implications For Recreational Fisheries Management, F. B. Prokop

Fisheries Management Papers

Workshop at Second National Fisheries Managers Conference, Bribie Island, Queensland, October 18, 1994. Recreational fisheries management is one of the growth areas in Australian fisheries. The Australian Society for Fish Biology Workshop topic for its 1994 Conference was Recreational Fishing - What's the Catch. It was acknowledged at the ASFB conference that there were generally poor levels of information and for the lower value commercial and recreational fisheries there may be no way to gather definitive research data for the foreseeable future. However, in even the high profile fisheries, public perceptions, attitudes, and political will do not allow for the …

Draft Report Of The South Coast Estuarine Fishery Working Group, South Coast Estuarine Fishery Workng Group

Fisheries Management Papers

The South Coast Estuarine Working Group was established in 1991 at the request of commercial fishermen by the Minister of the day. This report outlines a draft management plan for the South Coast Estuarine fishery that was made available for public comment.

Enhancer Trap Technique: A Novel Tool For Identification And Developmental Characterization Of Genes Of Drosophila, Amit Singh

Biology Faculty Publications

The classical technique of mutational screen for identification of genes controlling early development has now approached saturation. A new era in genetic identification and developmental characterization of genes in Drosophila has commenced with the advent of the enhancer trap technique. This technique involves mobilization of a P-lacZ vector to diverse chromosomal locations in the fruit fly genome to bring it under the regulation of developmentally expressed genes or their enhancer elements. The technique offers a strikingly elegant method of gaining entry into fruit fly genes.

Implications Of Native Title Legislation For Fisheries Management And The Fishing Industry In Western Australia, Patricia Summerfield

Fisheries Management Papers

This paper seeks to examine the key issues of both State and Commonwealth native title legislation for the fishing industry and fisheries management in Western Australia. Considerations for Aboriginal involvement in the industry and long-standing as well as recent concessionary arrangements for traditional usage of fish resources are examined. Industry reactions and future plans to accommodate native title in a positive framework are explored.

An Improved Method For Chemical Devitellinization Of X-Gal Stained Drosophila Embryos, Amit Singh, Madhuri Kango-Singh, P. Sinha

Biology Faculty Publications

In Drosophila developmental biological studies, X-gal staining is commonly employed to study the spatio-temporal expression of the lacZ reporter gene in the transformed flies or their embryos. Study of the lacZ pattern in embryos often suffers from the lack of an efficient and high yieldirrg technique for devitellinization of X-gal stained embryos. Devitellinization techniques employed during antibody staining, in situ hybridization or embryonic cuticular preparations generally do not give satisfactory results when used for similar purpose in X-gal stained embryos. This results in the flaky appearance of the blue stain. We present here an improved chemical devitellinization technique which gives …

Shark Bay World Heritage Area Draft Management Plan For Fish Resources, Fisheries Department Of Western Australia

Fisheries Management Papers

Shark Bay was inscribed on the World Heritage List in December 1991 on the basis of its natural values. The Department of Conservation and Land Management (CALM) is the management agency with responsibility for the oversight and development of management plans dealing with both the marine and terrestrial aspects of the World Heritage Area. This Draft plan deals with the management of fish resources in the Shark Bay World Heritage Area which are the responsibility of the Fisheries Department of Western Australia.

Rock Lobster Industry Advisory Commitee Chairman's Report, October 1994 - The Western Rock Lobster Fishery - Management Proposals For The 1994/95 And 1995/96 Seasons, Rock Lobster Industry Advisory Committee, Peter Rogers

Fisheries Management Papers

The failures of past restrictions on fishing effort to fully halt the decline of the breeding stock led to the formation of a sub-committee of the Rock Lobster Industry Advisory Committee. The prime objective of the sub-committee is to evaluate effective long term strategies for managing the fishery so that breeding stock is maintained at an adequate level despite the inevitable increases in catching efficiency. The purpose of this report is firstly to advise industry of the management proposals for the 1994/95 seasons and secondly to form a basis for industry consultation on management arrangements for the 1995/96 season.

Long Term Management Strategies For The Western Rock Lobster Fishery (4 Volumes) - Law Enforcement Considerations (Volume 4), Neil Mclaughlan

Fisheries Management Papers

This report has been prepared for the consideration of the RLIAC Sub Committee in consultation with members of the Sub Committee working group on law enforcement and with senior staff of the Operations Division of the Fisheries Department. It is focussed exclusively on law enforcement issues associated with the management options for the West Australian west coast rock lobster fishery which were under consideration by the Sub Committee at the time of report preparation and should be read in conjunction with Fisheries Management Paper No. 67 entitled "Evaluation of management options" which covers the broader industry management issues. This report …

Long Term Management Strategies For The Western Rock Lobster Fishery (4 Volumes) - A Market-Based Economic Assessment For The Western Rock Lobster Industry (May 1994) (Volume 3), Marec Pty Ltd

Fisheries Management Papers

In October 1992 a special Sub-Committee of the Rock Lobster Industry Advisory Committee was set up to consider and evaluate future management strategies for the rock lobster fishery. Pursuant to this the Director of Fisheries commissioned a marketing study that is the basis of this report. This study was commenced in early October 1993 and completed in May 1994. This study looked at the market implications of fishery's management strategies. In particular it assesses how the existing management structure limits the potential for the lobster industry to maximise the financial return, and in doing so provides an insight into the …

Long Term Management Strategies For The Western Rock Lobster Industry (4 Volumes) - Economic Efficiency Of Alternative Input And Output Based Management Systems In The Western Rock Lobster Fishery (Volume 2), Bob Lindner

Fisheries Management Papers

Since 1965, the Western Rock Lobster Fishery has been managed by what is essentially a license limitation scheme invoiving restricted entry for boats, and strict controls on the aggregate number of pots which can be used by the commercial fishing sector. By most measures, management of this fishery has been highly successful. Biological over-exploitation of the fish stock has been prevented, at least until recently. Notwithstanding these achievements, there is widespread concern that the future profitability, and even the viability of the industry is under threat unless the current management practices are reformed. Because of the complexity and intrinsic uncertainty …

Long Term Management Strategies For The Western Rock Lobster (4 Volumes) - Evaluation Of Management Options (Volume 1), B. K. Bowen, Rock Lobster Industry Advisory Committee

Fisheries Management Papers

This report has been written following approval by the Minister for Fisheries of a recommendation by the Rock LObster Industry Advisory Committee that a major evaluation of future management options for the western rock lobster fishery be undertaken. The report provides background information on the management strategies and principles of the fishery, its stock status, the use of input and output controls and the size of the fishing fleet. The report concludes with an examination of the effect of maintaining effort (input) controls and moving to a system of variable effort (TAE/ITEs) or moving to direct (output) controls on the …

Future Of Management Of Recreational Gill, Haul, And Cast Netting In Western Australia And Summary Of Submissions To The Netting Review, F. B. Prokop, L. M. Adams

Fisheries Management Papers

The results of a major review into recreational gill and haul netting in Western Australia were announced by the Minister for Fisheries, Hon. Monty House MLA on 24 December 1993. This document presents the findings of the recreational netting review and the process which resulted in the announced changed to management practices.

Development And Application Of A Thermistor Current Meter, Carl M. Way, Albert J. Burky, Christine Miller-Way

Biology Faculty Publications

This report provides details for the construction of a hot-bead thermistor current meter which is capable of measuring water velocities on a millimeter spatial scale and for the construction of a compact and accurate calibration system. Hot-bead thermistor current meters can be built with response times of 200 ms capable of measuring velocities between 0.1 and 80 cm s-1. The construction of a sturdy probe for application in lotic systems such as high gradient Hawaiian streams was achieved by the use of heavy-duty acrylic tubing, small stainless steel gas-chromatography tubing, and flexible Tygon spaghetti tubing. An acrylic handle anchors the …

The Introduction And Translocation Of Fish, Crustaceans And Molluscs In Western Australia., Craig Lawrence

Fisheries Management Papers

This paper discusses three possible impacts of introduced and translocated aquatic organisms: 1. Genetic diversity 2. Pathogens 3 Ecosystem Conservation, in relation to costs and benefits to the community and the Western Australian environment.

Biochemical Systematics Of Notothenioid Fishes From Antarctica, Mara A. Mcdonald, Michael H. Smith, Michael W. Smith, James M. Novak, Paul E. Johns, Arthur L. Devries

Faculty Research & Creative Activity

Genetic variation at 30 protein-coding loci was examined in seven forms of notothenioid fishes from Antarctica. Multilocus heterozygosity varied from 0.018 to 0.078 across taxa. An analysis of the allozyme data revealed the probable existence of an unrecognized cryptic species within Trematomu5 bemacchii. Pagothenia borchgrevinki is as closely related to some species of Trematomus as are some species of Trematomus to each other. Speciation among the species of Trematomus and Pagothenia appears to have taken place primarily after the separation of Antarctica from Australia.

The Future For Recreational Fishing Final Report Of The Recreational Fishing Advisory Committee, Recreational Fishing Advisory Committee

Fisheries Management Papers

This report discusses: What Recreational fishing means to Western Australia's estimated 300,000 fishers. Conservation and equality in catch sharing was at the heart of the Committee's investigations into developing strategies for the future management of important recreational fish stocks.

Micronuclear Genome Organization In Euplotes Crassus: A Transposonlike Element Is Removed During Macronuclear Development, Scott Everet Baird, Gina M. Fino, Susan L. Tausta, Lawrence A. Klobutcher

Biological Sciences Faculty Publications

After mating, hypotrichous ciliated protozoa transform a set of their micronuclear chromosomes into thousands of short, linear DNA molecules that form the macronuclear genome. To examine micronuclear genome organization in the hypotrich Euplotes crassus, we have analyzed two cloned segments of micronuclear DNA as well as the macronuclear DNA molecules that are derived from them. E. crassus was found to display a number of features characteristic of other hypotrich genomes, including (i) clustering and close spacing of the precursors of macronuclear DNA molecules, (ii) the frequent occurrence of internal eliminated sequences within macronuclear precursors, (iii) overlapping macronuclear precursors, (iv) lack …

The Paradigm Of Huntington Disease, John B. Jenkins, P. M. Conneally

Biology Faculty Works

No abstract provided.

Southern Demersal Gillnet And Longline Fishery., Management Advisory Committee

Fisheries Management Papers

In its report to the Ministier for Fisheries in November 1986, the Southern Western Australian Shark Working Group recommended the establishment of an Industry/Government Mangement Advisory Committee to provide managememt advice on the fishery to the Minister for Fisheries. The Advisory Committee held its first meeting in Albany on 23 February 1989. After being briefed on the state of shark stocks, the Committee's deliberations fell into three broad categories. In the first category there was sufficient time and information available to make a recommendation to the Minister for Fisheries. In the second category, discussion commenced, but was held over to …

Analysis Of Mitochondrial Dna Restriction Fragment Patterns In Killer Whales, Orcinus Orca, Tracy Alison Stevens

Dissertations and Theses

The mitochondrial DNA restriction fragment patterns of killer whales (Orcinus orca) were investigated in order to determine the level of genetic differentiation that exists between killer whales from various geographic locations. Twenty one killer whales were examined, seventeen of which were captive killer whales that originated from the North Atlantic and Northeast Pacific Oceans. Two were captive-born animals and two were killer whales that stranded along the Northeast Pacific coast.

DNA was extracted from blood and/or tissue samples, cleaved with a variety of restriction endonucleases and the DNA fragments were separated by horizontal agarose gel electrophoresis. The DNA …

Rock Lobster Industry Advisory Committee Report To The Minister For Fisheries., Rock Lobster Industry Advisory Committee

Fisheries Management Papers

The total catch of about 12.1 million kilograms for the 1987/88 season, based on processor returns, was an increase of 44% on the 1986/87 season. This very high catch, the third highest on record, resulted from an above average perulus settlement in 1983/84 and a "flow-on" of fast growing animals stemming from the expected high recruitment in 1988/990 caught as "reds" in 1987/88.

The Committee has identified five major elements related to the state of the rock lobster fishery upon which management advice is considered.

a) The undersize fraction of the population.

b) The "whites" which provide a significant proportion …

Characterization Of Chromosome Fragmentation In Two Protozoans And Identification Of A Candidate Fragmentation Sequence In Euplotes Crassus, Scott Everet Baird, Lawrence A. Klobutcher

Biological Sciences Faculty Publications

Following the sexual cycle, hypotrichous ciliated protozoans fragment a set of their micronuclear chromosomes to generate the thousands of short, linear DNA molecules present in the transcriptionally active macronucleus. We have used a hybrid selection procedure to examine macronuclear DNA molecules for subtelomeric length heterogeneity to determine whether chromosome fragmentation occurs at unique or multiple sites. The results suggest that multiple, but closely spaced, chromosome fragmentation sites are used by Oxytricha nova. In contrast, Euplotes crassus uses unique chromosome fragmentation sites in a reproducible manner to generate the ends of macronuclear DNA molecules. Additional studies compared DNA sequences in …

Genic Population Structure And Gene Flow In The Northern Flicker (Colaptes Auratus) Hybrid Zone, Thaddeus A. Grudzien, William S. Moore, J. Richard Cook, Danilo Tagle

Biological Sciences Faculty Research Publications

The Yellow-shafted Flicker (Colaptes auratus auratus) and Red-shafted Flicker (C. a. cafer) form a stable, narrow hybrid zone on the western Great Plains of North America. Allozyme data were obtained from 31 structural gene loci for 33 samples representing 246 Northern Flickers from throughout the Great Plains. Flickers were approximately equivalent to other birds in terms of proportion of polymorphic loci (P = 0.207) and average heterozygosity (H = 0.056). There was no concordant variation between plumage characters and allelic frequencies.

Gene-diversity analysis indicated that 92.5% of the genic variation occurred as within-deme heterozygosity ( …

The Eugenics Record Office At Cold Spring Harbor, 1910-1940: An Essay In Institutional History, Garland E. Allen

Biology Faculty Research

No abstract provided.

Seasonal Responses Of Filtration Rates To Temperature, Oxygen Availability, And Particle Concentration Of The Freshwater Clam, Musculium Partumeium (Say), Albert J. Burky, Richard B. Benjamin, Denis G. Conover, Jennifer Detrick

Biology Faculty Publications

Seasonal filtration rates of a pond population of the freshwater pisidiid clam, Musculium par­tumeium (Say), were assessed by measuring the clearance of 2.02 µ.m latex beads from suspension and expressed in terms of FR = a(AFDW}b where FR = filtration rate (ml H20 · hr- 1 ) , AFDW = mg ash-free dry weight of a whole clam and "a" and "b" are constants. The a-values ( = FR of a 1-mg AFDW clam} vary seasonally with the highest rates corresponding to periods of maximum growth and reproduction in the spring and fall. Changes in "b" reflect the influence of …

Review Of "The Nature And Origin Of The Biological World" By E. J. Ambrose, John B. Jenkins

Biology Faculty Works

No abstract provided.

Natural Killer Activity In Gardner's Syndrome, Ann Marie Stembridge

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Gardner's syndrome is an autosomal dominant disease presenting multiple colonic polyps with a predisposition for malignant change. In addition to colonic polyp formation by early adolescence, extracolonic lesions appear often prior to polyp formation. One theoretical mechanism for the origin of polyps and malignancies in Gardner's syndrome is a genetic defect in the natural killer cell activity of patients with this disease. Natural killer cells are a subpopulation of lymphocytes that spontaneously lyse tumor cells and virally transformed cells. A study was undertaken to determine the natural killer activity of patients with Gardner's syndrome.

A technique termed chromium release was …

Synthesis Of Low Molecular Weight Heat Shock Peptides Stimulated By Ecdysterone In A Cultured Drosophila Cell Line., Robert C. Ireland, Edward M. Berger

Dartmouth Scholarship

Treatment of Schneider's line 3 Drosophila cells with the steroid hormone ecdysterone rapidly stimulated the synthesis and accumulation of the polypeptide previously designated p7 [Berger, E. M., Ireland, R. C. & Wyss, C. (1980) Somatic Cell Genet. 6, 119-129]. In this report, p7 is identified as the 23,000-dalton heat shock polypeptide (hsp23). In addition to hsp23, the synthesis of the low molecular weight heat shock polypeptides hsp22, hsp26, and hsp27 was also stimulated by ecdysterone, although to different extents. Hybridization of a nick-translated genomic clone containing the hsp23 gene to a total RNA blot showed that ecdysterone stimulation of hsp23 …