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Articles 1261 - 1290 of 3461

Full-Text Articles in Biology

Teaching Natural And Artificial Selection In Production Agriculture, Madhav P. Nepal, Clayton W. Scott Mar 2020

Teaching Natural And Artificial Selection In Production Agriculture, Madhav P. Nepal, Clayton W. Scott

iLEARN Teaching Resources

In this lesson, students will learn how natural selection and artificial selection impact both production agriculture and biological sciences. A natural selection is a mechanism that favors heritable traits that increase species survival and reproduction. Artificial selection is a selective breeding, where humans select for desirable traits in agricultural products.


Do Foliar Endophytes Matter In Litter Decomposition?, Emily R. Wolfe, Daniel J. Ballhorn Mar 2020

Do Foliar Endophytes Matter In Litter Decomposition?, Emily R. Wolfe, Daniel J. Ballhorn

Biology Faculty Publications and Presentations

Litter decomposition rates are affected by a variety of abiotic and biotic factors, including the presence of fungal endophytes in host plant tissues. This review broadly analyzes the findings of 67 studies on the roles of foliar endophytes in litter decomposition, and their effects on decomposition rates. From 29 studies and 1 review, we compiled a comprehensive table of 710 leaf-associated fungal taxa, including the type of tissue these taxa were associated with and isolated from, whether they were reported as endo- or epiphytic, and whether they had reported saprophytic abilities. Aquatic (i.e., in-stream) decomposition studies of endophyte-affected litter were …


The Mangroves Of Costa Rica, Kylie Stewart Feb 2020

The Mangroves Of Costa Rica, Kylie Stewart

Honors Projects

Mangroves are a type of coastal vegetation present in the intertidal zone of tropical and subtropical climates. This paper explores the mangroves of Costa Rica, their significance, and various strategies the country utilizes in order to protect mangrove forests. Several books and articles about mangroves and conservationism are examined to explain why mangrove forests are vital to a coastline’s health and growth. The scholarly topic pertains to mangroves located in the United States and Costa Rica along with methods used to maintain the wildlife that live within them. The concept of ecotourism will be explored and how mangrove forests are …


Isotope-Based Methods For Evaluating Fish Trophic Geographies, Julie L. Vecchio Feb 2020

Isotope-Based Methods For Evaluating Fish Trophic Geographies, Julie L. Vecchio

USF Tampa Graduate Theses and Dissertations

Data on the movement and diets of fish during a variety of life stages are important inputs to fisheries stock assessments and marine ecosystem models. Stable isotopes may provide previously inaccessible information on movement and diet of a variety of managed and forage fish species. Here I used several novel means of interpretation for stable isotope data to infer diets and movements of several important fisheries species over both short (weeks to months) and long (lifetime) timescales. To calculate a constant partitioning offset (CPO) between the δ15N of muscle and of liver tissue, I conducted a literature search …


The Role Of Dislodgement In The Territorial Ecology Of The Owl Limpet, Lottia Gigantea, Ryan T. Kabala, Natalie Swinford, Maria J. Mason, William G. Wright Feb 2020

The Role Of Dislodgement In The Territorial Ecology Of The Owl Limpet, Lottia Gigantea, Ryan T. Kabala, Natalie Swinford, Maria J. Mason, William G. Wright

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Ascertaining the risks and benefits of different behaviors is a central goal of research on territoriality. Although most territorial behavior is ritualized, with concomitant reduced risks for both residents and intruders, this ritualization is generally found to be underpinned by rare, highly consequential, interactions. The agonistic behavior of the intertidal owl limpet, Lottia gigantea, involves defense of a feeding territory, and includes a relatively explosive thrusting response by territory holders against intruding conspecifics. We here ask whether this thrusting behavior is capable of entirely dislodging intruders from their rocky wave-swept substratum, thereby ridding the resident of future challenges by …


Range Extension For Dekay’S Brownsnake (Storeria Dekayi) In South-Central Nebraska, Macy K. Cool, Keith Geluso Feb 2020

Range Extension For Dekay’S Brownsnake (Storeria Dekayi) In South-Central Nebraska, Macy K. Cool, Keith Geluso

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

Dekay’s Brownsnake (Storeria dekayi) generally occurs in southeastern parts of the state, where the species was known from 16 counties. Herein, we update the distribution of S. dekayi in the state and briefly comment on habitat and abundance from observations in September and October 2018. We documented 7 new county records that extend its distribution in south-central Nebraska. We observed that S. dekayi does not only reside in mesic wooded habitats in Nebraska but also occupies open environments, including areas with upland grasslands and row-crop agriculture. Dekay’s Brownsnake currently is listed as a species of concern …


Are Humans Natural? Part 4: Human-Nature Relational Values Through Time, Nathan Ruhl, Sirena Pimenta Feb 2020

Are Humans Natural? Part 4: Human-Nature Relational Values Through Time, Nathan Ruhl, Sirena Pimenta

Open Educational Resources

This activity assumes students have a background in evolutionary theory, so students without such a background should have additional instruction prior to conducting this part of the activity. Consider a discussion of mechanisms that drive evolution, including genetic drift, natural selection, and gene flow. Evolution is a change in gene frequencies in a population over the course of several generations.

Genes are the genetic code controlling many individual characteristics or traits, so the frequency at which genes occur dictates, in large part, the frequency at which individual characteristics or traits exist in a population. The frequency of a gene can …


Statistical Analysis Of Nutrient Loads From The Mississippi-Atchafalaya River Basin (Marb) To The Gulf Of Mexico, Phyllis Okwan, Yi Zhen, Huan Feng, Shinjae Yoo, Murty S. Kambhampati, Abreione Walker, Shayne Boykin, Joe Omojola, Noel Blackburn Jan 2020

Statistical Analysis Of Nutrient Loads From The Mississippi-Atchafalaya River Basin (Marb) To The Gulf Of Mexico, Phyllis Okwan, Yi Zhen, Huan Feng, Shinjae Yoo, Murty S. Kambhampati, Abreione Walker, Shayne Boykin, Joe Omojola, Noel Blackburn

Department of Earth and Environmental Studies Faculty Scholarship and Creative Works

This study investigated the annual and seasonal variations in nutrient loads (NO2− + NO3− and orthophosphate) delivered to the Gulf of Mexico from the Mississippi-Atchafalaya River Basin (MARB) and examined the water quality variations. The results indicate that (1) annually, the mean NO2− + NO3− and orthophosphate loads showed a steady increase during 1996–1999, a persistent level during 2000–2007, and a moderate increase during 2008–2016; (2) seasonally, NO2− + NO3− and orthophosphate in MARB in spring and summer were higher than those in autumn and winter. Analysis of …


The Bioethical Significance Of “The Origin Of Man’S Ethical Behavior” (October 1941, Unpublished) By Ernest Everett Just And Hedwig Anna Schnetzler Just, Theodore Walker Jr. Jan 2020

The Bioethical Significance Of “The Origin Of Man’S Ethical Behavior” (October 1941, Unpublished) By Ernest Everett Just And Hedwig Anna Schnetzler Just, Theodore Walker Jr.

Journal of the South Carolina Academy of Science

Abstract –

E. E. Just (1883-1941) is an acknowledged “pioneer” in cell biology, and he is perhaps the pioneer in study of egg cell fertilization. Here we discover that Just also made pioneering contributions to general biology and evolutionary bioethics.

Within Just’s published contributions to observational cell biology, there are substantial fragments of his theory of ethical behavior, a theory with roots in cell biology. In addition to such previously available fragments, Just’s fully developed theory is now available. This recently discovered unpublished book-length manuscript argues for the biological origins of ethical behavior (evolving from cells to humans, within a …


The Undergraduate Student’S Guide To Geometric Morphometrics, Erika Crispo Jan 2020

The Undergraduate Student’S Guide To Geometric Morphometrics, Erika Crispo

Open Educational Resources

Embarking on a new research endeavor can be a daunting task. User guides, books, and published articles are written for an audience that already has some background experience in the field. Undergraduate students like you, who are at the very beginning of their research careers, often struggle to make sense of these documents. Furthermore, students like you often attempt to do so while balancing heavy course loads. Thus, I have written this document to help ease the burden so that you have more time to ponder the interesting scientific questions instead of digging through pages upon pages of documentation. I …


Effects Of A Neonicotinoid Insecticide On The Growth Of Honey Bee Gut Microbes, Macee Mitchell, Daniel Franzese, Taylor Morales, Shane Lucht, Jesse Steele, Jenifer Walke Jan 2020

Effects Of A Neonicotinoid Insecticide On The Growth Of Honey Bee Gut Microbes, Macee Mitchell, Daniel Franzese, Taylor Morales, Shane Lucht, Jesse Steele, Jenifer Walke

2020 Symposium Posters

The gut microbiome plays an essential role in the overall health of organisms. However, the presence and abundance of these microbes may be altered by environmental factors, such as exposure to pesticides. The goals of this project were to understand 1) the prevalence of pesticide residues in honey bees in eastern Washington, and 2) the impact of pesticides to the honey bee gut microbiome. Accordingly, we tested bees from 24 hives among six sites across eastern Washington. High-performance liquid chromatography-mass spectrometry (HPLC-MS) was used to detect the presence and concentration of six commonly used agricultural pesticides: Carbaryl, Chlorpyrifos, Coumaphos, Cypermethrin, …


Impact Of Nacl Tolerance On The Stress Physiology And Fitness Of Larval Wood Frogs (Rana Sylvatica), Grascen Shidemantle, Nicholas Buss, Jessica Hua Jan 2020

Impact Of Nacl Tolerance On The Stress Physiology And Fitness Of Larval Wood Frogs (Rana Sylvatica), Grascen Shidemantle, Nicholas Buss, Jessica Hua

Biological Sciences Student Scholarship

No abstract provided.


A Literature Review Of Horn And Horn-Like Structures In Vertebrates To Correlate Placement To Function, Behavior, And Niche., J T. Cottingham Jan 2020

A Literature Review Of Horn And Horn-Like Structures In Vertebrates To Correlate Placement To Function, Behavior, And Niche., J T. Cottingham

Biology Papers and Presentations

In this paper, the true horns of bovids are compared to the many horn-like structures found throughout the animal kingdom. Literary sources were reviewed for a variety of horn-like structures. The review was limited to extinct and extant terrestrial vertebrates. 3D models of these organisms' skulls and “horns” were created or acquired and 3D printed at the same scale to illustrate their differences and similarities and to analysis of their comparative size easier between large and small genera.


Seedling Survival Declines With Increasing Conspecific Density In A Common Temperate Tree, Fiona V. Jevon, Sydne Record, John Grady, Ashley K. Lang, David A. Orwig, Matthew P. Ayres, Jaclyn H. Matthes Jan 2020

Seedling Survival Declines With Increasing Conspecific Density In A Common Temperate Tree, Fiona V. Jevon, Sydne Record, John Grady, Ashley K. Lang, David A. Orwig, Matthew P. Ayres, Jaclyn H. Matthes

Biology Faculty Research and Scholarship

Feedbacks between plants and their soil microbial communities often drive negative density dependence in rare, tropical tree species, but their importance to common, temperate trees remains unclear. Additionally, whether negative density dependence is driven by natural enemies (e.g., soil pathogens) or by high densities of seedlings has rarely been assessed. Density dependence may also depend on seedling size, as smaller and/or younger seedlings may be more susceptible to mortality agents. We monitored seedlings of Quercus rubra, a common, canopy‐dominant temperate tree, to investigate how the density of neighboring adults and seedlings influenced their survival over two years. We assessed …


Rodent Seed Preference Trials Of Ecologically Important Plants In Kentucky, Katelyn Thomason Jan 2020

Rodent Seed Preference Trials Of Ecologically Important Plants In Kentucky, Katelyn Thomason

Mahurin Honors College Capstone Experience/Thesis Projects

Seed predation is an important ecological process that can affect the success of individual plants and plant populations as a whole. However, seed preferences of herbivores have received little detailed attention in comparison to seedling predation studies (Hulme, 1994). In this project I researched how rodents, specifically their seed preferences, affect the recruitment and survival of ecologically important native plants in Western Kentucky. I used traps to catch Peromyscus leucopus (white-footed mice). Once caught, each mouse was placed in a trial arena with seeds from Silphium perfoliatum (cup plant), Baptisia australis (blue false indigo), Silphium lanciniatum (compass plant), and the …


Morphological Correlates Of Auditory Sensitivity In The Inner Ear Of Two Species Of Invasive Carp, Tyler Patty Jan 2020

Morphological Correlates Of Auditory Sensitivity In The Inner Ear Of Two Species Of Invasive Carp, Tyler Patty

Mahurin Honors College Capstone Experience/Thesis Projects

Silver (Hypopthalmichthys molitrix) and bighead (H. nobilis) carp, are invasive species that have negative impacts upon ecosystems. H. molitrix is known to jump completely out of the water in response to broadband sounds, however, this is not observed in H. nobilis. Preliminary experiments reveal that sounds can be used to modify the behavior of carps. Thus, understanding the hearing abilities of these species is important in order to design appropriate acoustical deterrents. Fish heads were preserved in 4% paraformaldehyde and the inner ears dissected and photographed under a light microscope in order to describe the …


Use And Partitioning Of Riparian Corridors By Mammalian Carnivores In Rio Mora National Wildlife Refuge, Armando Toral Becker Jan 2020

Use And Partitioning Of Riparian Corridors By Mammalian Carnivores In Rio Mora National Wildlife Refuge, Armando Toral Becker

Regis University Student Publications (comprehensive collection)

Land conversion across the Southwestern U.S. for agricultural and livestock use and prolonged drought conditions cascade to reduce ecosystem function, including habitat provision for wildlife. Arroyo restoration efforts aim to improve wildlife habitat by decreasing soil erosion and enhancing water retention. These hydrologic and geomorphic changes increase the likelihood that wildlife use restored corridors by increasing riparian vegetation cover. Rio Mora National Wildlife Refuge (RMNWR) has restored several arroyos by installing a system of one-rock dams throughout the refuge, but the extent to which mammals prefer restored over unrestored riparian corridors and how habitat structure influences mammalian use and density …


Ticks On Lizards: Parasite-Host Interactions Of The Southern Alligator Lizard (Elgaria Multicarinata) In Washington State, Emma Houghton Jan 2020

Ticks On Lizards: Parasite-Host Interactions Of The Southern Alligator Lizard (Elgaria Multicarinata) In Washington State, Emma Houghton

Undergraduate Honors Theses

I investigated interactions between ectoparasites (ticks) and their host, the southern alligator lizard (Elgaria multicarinata). Alligator lizards are capable of complement-mediated killing of the Lyme disease spirochete carried by ticks and may potentially reduce Lyme disease prevalence by cleansing pathogenic organisms from ticks. Despite this, little is known about host-parasite dynamics in alligator lizards. My goals were to 1) assess patterns of tick presence (i.e. parasite load) on alligator lizards and 2) investigate potential negative effects of ticks on alligator lizards. I sampled lizards during the summer of 2019 near Catherine Creek, along the Columbia River Gorge in southern Washington. …


Investigating The Avian Species Composition, Diversity, And Abundance Of Engelhorn Pond, Kiana Rose Jan 2020

Investigating The Avian Species Composition, Diversity, And Abundance Of Engelhorn Pond, Kiana Rose

Undergraduate Honors Theses

Central Washington University’s Engelhorn Pond contains important habitats for many organisms, particularly migratory birds, and is a valuable resource for students. However, little information is available about the wildlife inhabiting the pond. The pond’s discrete location means that many people are unaware of its existence. In addition, the pond is threatened by a number of anthropogenic sources, including chemical runoff, garbage, and feral cats. By using point counts, a common ornithological survey technique, I recorded the bird species present at the pond during all seasons of the year. I collected data using point counts during 13 visits from June of …


Ticks On Lizards: Parasite-Host Interactions Of The Southern Alligator Lizard (Elgaria Multicarinata) In Washington State, Emma Houghton Jan 2020

Ticks On Lizards: Parasite-Host Interactions Of The Southern Alligator Lizard (Elgaria Multicarinata) In Washington State, Emma Houghton

All Undergraduate Projects

I investigated interactions between ectoparasites (ticks) and their host, the southern alligator lizard (Elgaria multicarinata). Alligator lizards are capable of complement-mediated killing of the Lyme disease spirochete carried by ticks and may potentially reduce Lyme disease prevalence by cleansing pathogenic organisms from ticks. Despite this, little is known about host-parasite dynamics in alligator lizards. My goals were to 1) assess patterns of tick presence (i.e. parasite load) on alligator lizards and 2) investigate potential negative effects of ticks on alligator lizards. I sampled lizards during the summer of 2019 near Catherine Creek, along the Columbia River Gorge in southern Washington. …


Temporal Variability Of Polycyclic Aromatic Hydrocarbons In Deep-Sea Cephalopods Of The Northern Gulf Of Mexico, Isabel C. Romero, Heather L. Judkins, Michael Vecchione Jan 2020

Temporal Variability Of Polycyclic Aromatic Hydrocarbons In Deep-Sea Cephalopods Of The Northern Gulf Of Mexico, Isabel C. Romero, Heather L. Judkins, Michael Vecchione

USF St. Petersburg Campus Faculty Publications

As part of the effort to understand the effects of the Deepwater Horizon Oil Spill (DWHOS), we analyzed tissue from five species of midwater oceanic cephalopods in the northern Gulf of Mexico (GoM) during three time periods, including one period sampled fortuitously just before the spill (2010), and two periods sampled after the spill (2011 and 2015–2016). The species, Japetella diaphana, Abralia redfieldi, Histioteuthis corona, Leachia atlantica, and Onychoteuthis banksii were collected in three geographic areas in the GoM (east, south and southeast of the Macondo wellhead). Results indicate a shift in the composition of polycyclic aromatic hydrocarbons (PAHs) in …


Using Northern Pike And Yellow Perch To Attempt A Trophic Cascade, Bryan Sea Jan 2020

Using Northern Pike And Yellow Perch To Attempt A Trophic Cascade, Bryan Sea

Journal of Earth and Life Science

In the midwestern USA, water clarity has decreased in shallow lakes as a result of large populations of Fathead Minnow Pimephales promelas and agriculture that impact the life that depend on shallow habitats, such as waterfowl, fish, and aquatic plants. Northern Pike Esox lucius and Yellow Perch Perca flavescens were stocked in a few lakes in attempt to create a tropic cascade and reduce populations of nuisance Fathead Minnows. Northern Pike were stocked as fry and Yellow Perch were stocked as adults either pre-spawn or post-spawn. Lake managers hypothesized adult Yellow Perch would spawn and the age-0 Perch would feed …


In Memoriam: Ignacio Ribera (1963–2020), Emmanuel D. Delocado, Michael Balke, Hendrik Freitag Jan 2020

In Memoriam: Ignacio Ribera (1963–2020), Emmanuel D. Delocado, Michael Balke, Hendrik Freitag

Biology Faculty Publications

No abstract provided.


Genetic Analysis Of The Endangered Gray Bat Species (Myotis Grisescens), Emma Fitzgerald Jan 2020

Genetic Analysis Of The Endangered Gray Bat Species (Myotis Grisescens), Emma Fitzgerald

Lewis Honors College Capstone Collection

This study attempts to analyze the genetics using specific haplotype sequences of the endangered gray bat to determine genetic events that have happened in the past as well as determining how the species population has changed over time. We were able to conduct a Tajima’s D test to investigate whether a potential bottleneck has occurred. A linear regression of the genetic vs. geographical distance was produced to investigate the patterns of haplotype distribution. The species past distribution was compared to the current distribution using known collected specimens. A map of future predictions was constructed using present and future climate layers …


Direct And Indirect Effects Of Sex Steroids On Gonadotrope Cell Plasticity In The Teleost Fish Pituitary, Romain Fontaine, Muhammad Rahmad Royan, Kristine Von Krogh, Finn-Arne Weltzien, Dianne M. Baker Jan 2020

Direct And Indirect Effects Of Sex Steroids On Gonadotrope Cell Plasticity In The Teleost Fish Pituitary, Romain Fontaine, Muhammad Rahmad Royan, Kristine Von Krogh, Finn-Arne Weltzien, Dianne M. Baker

Biological Sciences Research

The pituitary gland controls many important physiological processes in vertebrates, including growth, homeostasis, and reproduction. As in mammals, the teleost pituitary exhibits a high degree of plasticity. This plasticity permits changes in hormone production and secretion necessary to meet the fluctuating demands over the life of an animal. Pituitary plasticity is achieved at both cellular and population levels. At the cellular level, hormone synthesis and release can be regulated via changes in cell composition to modulate both sensitivity and response to different signals. At the cell population level, the number of cells producing a given hormone can change due to …


Demonstrating Mate Choice Copying In Spiders Requires Further Research, R. Tucker Gilman, Kasey Fowler-Finn, Eileen A. Hebets Jan 2020

Demonstrating Mate Choice Copying In Spiders Requires Further Research, R. Tucker Gilman, Kasey Fowler-Finn, Eileen A. Hebets

Eileen Hebets Publications

Mate choice copying—when individuals learn to prefer mates or mate types that have been chosen by others—can influence trait evo-lution and speciation (Varela et al. 2018; Dion et al. 2019). Most examples of mate choice copying are from fish, birds, and mammals including humans (Varela et al. 2018). However, 2 invertebrate examples—fruit flies and wolf spiders—have been used to argue that the phenomenon may be phylogenetically widespread, and perhaps the rule rather than the exception in nature (Varela et al. 2018). Here, we revisit the evidence for mate choice copying in wolf spiders (Fowler-Finn et al. 2015) in light of …


Examining A Fish Community And Its Response To Coastal Restoration In A Dynamic Coastal Estuary, Richard Mahoney Jan 2020

Examining A Fish Community And Its Response To Coastal Restoration In A Dynamic Coastal Estuary, Richard Mahoney

Electronic Theses and Dissertations, 2020-2023

Globally, coastal habitats are experiencing degradation due to the increase of human population growth, development along coastlines, and a constantly changing climate. This threatens the future production of critical ecosystem services such as shoreline stabilization, water filtration, nursery grounds for marine fauna, and many more. To combat these losses, resource managers are actively restoring coastal habitat. Past research suggests restoring habitats has mixed results; numerous factors influence restoration success. This study is among the first to assess the nekton community in the Matanzas River estuary and uses a BACI experimental design to quantify the effect of habitat restoration on the …


Evolutionary Ecology Of The Native Johnny Darter (Etheostoma Nigrum) And The Invasive Round Goby (Neogobius Melanostomus): A Genomic Perspective, Abby Joyce Wicks Jan 2020

Evolutionary Ecology Of The Native Johnny Darter (Etheostoma Nigrum) And The Invasive Round Goby (Neogobius Melanostomus): A Genomic Perspective, Abby Joyce Wicks

Wayne State University Dissertations

Round goby (Neogobius melanostomus) is an invasive fish present in all five Great Lakes and is becoming increasingly common in their tributaries. Johnny darter (Etheostoma nigrum) is a native species that often coexists with round goby. Here, I use traditional population genetic methods and genomic tools to explore the evolutionary ecology of these species. First, historic factors are addressed as a source of variation in study populations by characterizing patterns of mitochondrial DNA variation throughout Lower Michigan. Round goby populations were largely homogenous and exhibited no evidence of overarching historical genetic structure, consistent with the recent invasion and rapid expansion …


Haemoparasite Infection And Immunogenetic Diversity In A Clade Of North American Songbirds, Naima Starkloff Jan 2020

Haemoparasite Infection And Immunogenetic Diversity In A Clade Of North American Songbirds, Naima Starkloff

Legacy Theses & Dissertations (2009 - 2024)

The persistence of an organism in a particular habitat is mediated by a complex interplay of abiotic and biotic interactions. These interactions become especially complicated for a vector-transmitted parasite as its persistence relies on its ability to colonize both ectothermic and endothermic host organisms. Host factors, rather than environmental factors, have been highlighted as key predictors of the diversity and prevalence of avian haemosporidians (malaria parasites and relatives) globally. The range of a parasite is limited by its host specificity as well as the community of hosts present. In addition, as haemosporidian infections typically have negative fitness consequences on hosts, …


Molecular And Phenotypic Diversification Of A Cryptic Group Of Terrestrial Frogs From The Southern Andes Of Ecuador, Veronica Urgiles Penafiel Jan 2020

Molecular And Phenotypic Diversification Of A Cryptic Group Of Terrestrial Frogs From The Southern Andes Of Ecuador, Veronica Urgiles Penafiel

Electronic Theses and Dissertations, 2020-2023

The Ecuadorean Andes sustains one of the most remarkable frog diversifications. In this region, nearly one in three known species of amphibians belong to the Pristimantis genus, which contains the majority of the direct-developing terrestrial frog species. Although efforts are ongoing to understand the diversity of Pristimantis, large regions of the ecosystems they occupy remain understudied, and speciation and diversity of this genus remains poorly understood. Within this context, an interesting taxon with many unresolved questions regarding patterns and process of diversification is the Pristimantis orestes species complex which is distributed across the Paramo landscape and montane forests in the …