Biology Commons^™

Reciprocal Regulation Of Trek-1 Channels By Arachidonic Acid And Crh In Mouse Corticotropes, Andy K. Lee, James L. Smart, Marcelo Rubinstein, Malcolm J. Low, Amy Tse

Faculty Publications - Department of Biological & Molecular Science

Arachidonic acid (AA) is generated in the anterior pituitary gland upon stimulation by the ACTH secretagogue, CRH. Using the patch clamp technique,we examined the action of AA on the excitability of single pituitary corticotropes obtained from a transgenic mouse strain that expresses the enhanced green fluorescent protein driven by the proopiomelanocortin promoter. CRH evoked depolarization, but AA caused hyperpolarization. Under voltage clamp condition, AA caused a rapid inhibition of the delayed rectifier K current and then increased a background K current. Inhibition of AA metabolism did not prevent the activation of the K current by AA, suggesting a direct action …

Establishing An Interdepartmental Fluorescent Microscopy Facility, Marcy Kelly, David Zuzga, Nigel Yarlett

Cornerstone 3 Reports : Interdisciplinary Informatics

No abstract provided.

A Survey Of The Chemical Content And Medicinal Activity Of Shrub-Steppe Plants Of The Pacific Northwest, Aaron M. John

Undergraduate Honors Theses

A need for new modes of antibiotic therapy has resulted from the increasing incidence of bacterial resistance to drugs. One approach to this problem involves the search for plant compounds that may help to overcome multi-drug resistance. Many plant genera have been utilized in Native American traditional medicine for their beneficial properties. Such plants include the shrub-steppe genera, Eriogonun and Collomia, which have been used as cold remedies, pain relievers, and for antibiotic-related uses. Limited chemical studies of these genera have revealed medicinally useful compounds such as flavonoids and terpenoids, and further research may result in the identification of …

Investigating The Effects Of The Plasticizers Bisphenol A And Di (2-Ethylhexyl) Phthalate On Dopaminergic Neurons In A C. Elegans Parkinson's Disease, Amanda Valera

Undergraduate Honors Theses

Parkinson's disease (PD) is a neurodegenerative disorder characterized by loss of dopamine (DA) containing neurons in the substantia nigra of the brain. Though the cellular pathways that lead to PD are varied and not well understood, elevated levels of reactive oxygen species (ROS) and inhibition of the mitochondrial electron transport chain enzyme complex I have been associated with PD pathogenesis. Environmental factors such as the plasticizers bisphenol A (BPA) and di(2-ethylhexyl) phthalate (DEHP) may also be involved in PD pathogenesis. In this research, three strains of the nematode Caenorhabditis elegans (C. elegans), a wild-type N2 strain, a ROS-sensitive nnt …

Mechanisms Of Adsorption Of Copper And Silver Ions On Carbon Materials In An Open Circuit, Mikhail M. Goldin

Faculty Publications and Presentations

No abstract provided.

Effective Numbers Of Electrons As A Criterion Of Carbon Suitability As A Hemosorbent, Mogely Khubutiya, Boris Grafov, Mikhail M. Goldin, Alexei Davydov, Vladimir Kolesnikov, Mark Goldin

Faculty Publications and Presentations

The number of medical applications of electrochemistry has grown in recent years due to the increased applications of electrochemical concepts to various systems of the organism. This includes electrochemically controlled hemosorption detoxification, where the removal of toxicants is controlled by changing the potential of the hemosorbent. It is important to avoid Faradaic processes in the course of hemosorption, which can lead to the addition of electrochemically modified toxicants to blood. The probability of their occurrence should depend on the open-circuit potential of the activated carbon. In order to elucidate the identification of Faradaic reactions, a model system was investigated. The …

Table Of Contents

Journal of the South Carolina Academy of Science

No abstract provided.

President's Message, Justin K. Wyatt

Journal of the South Carolina Academy of Science

No abstract provided.

Scas Officers And Council Members 2010 - 2011

Journal of the South Carolina Academy of Science

No abstract provided.

Properties And Uses Of Sodium Titanates And Peroxotitanates, David Hobbs

Journal of the South Carolina Academy of Science

No abstract provided.

Applications Of Biotechnology In Development Of Biomaterials: Nanotechnology And Biofilms, Robin L. Brigmon, Christopher Berry, Raj N. Singh, Roger J. Narayan

Journal of the South Carolina Academy of Science

No abstract provided.

Nanocarriers For Biomedical Applications, Shuyi Li, Lynsa Nguyen, Hairong Xion, Meiyao Wang, Tom C.-C. Hu, Jin-Xiong She, Steven M. Serkiz, George G. Wicks, William S. Dynan

Journal of the South Carolina Academy of Science

No abstract provided.

Removal And Sequestration Of Iodide From Alkaline Solutions Using Silver-Doped Carbon Nanotubes, Adrian L. Pishko, Steven M. Serkiz, Apparao M. Rao

Journal of the South Carolina Academy of Science

No abstract provided.

Table Of Contents

Journal of the South Carolina Academy of Science

No abstract provided.

Scas Officers And Council Members 2010-2011

Journal of the South Carolina Academy of Science

No abstract provided.

Gold Nanospheres As Inhibitors Of Amyloid- Β Protein Aggregation Involved In Alzheimer’S Disease, Mihyun Lim, Deborah Soto-Ortega, Rahina Mahtab, Melissa Moss

Journal of the South Carolina Academy of Science

No abstract provided.

Sirna-Mediated Inhibition Of The Hiv-1 Transactivator Of Transcription, Alexander Jureka, Pricilla Simon, William Jackson

Journal of the South Carolina Academy of Science

No abstract provided.

Physiological Markers Of Activity-Based Anorexia In Sprague Dawley Rats, Crystal Lange, Michelle Vieyra

Journal of the South Carolina Academy of Science

No abstract provided.

Inverse Correspondence Of Amh And Fsh Levels In Women Presenting For Infertility Treatments, Amara Iverson, Abdelmonium Younis, William Butler, William Roudebush

Journal of the South Carolina Academy of Science

No abstract provided.

Folate Receptor-Targeted Delivery Of Small Interfering Rna To Cancer Cells, Yilin Zhang

Dissertations

The vitamin folic acid (folate, FA) has been extensively explored as a targeting ligand to deliver a variety of diagnostic/prognostic/therapeutic agents into various tumors through the assistance of its receptor – the folate receptor (FR). FR is over-expressed in many types of human cancer and can mediate internalization of FA-conjugates through an endocytic pathway. The discovery of small interfering RNA (siRNA), which is cable of inducing potent gene silencing in a sequence-specific manner, provides an excellent molecular tool to suppress aberrant gene expression in malignancies, and therefore siRNA has the potential to revolutionize cancer therapeutics. Towards the goal of developing …

Impact Of Interdisciplinary Undergraduate Research In Mathematics And Biology On The Development Of A New Course Integrating Five Stem Disciplines, Lester Caudill, April L. Hill, Kathy Hoke, Ovidiu Z. Lipan

Department of Math & Statistics Faculty Publications

Funded by innovative programs at the National Science Foundation and the Howard Hughes Medical Institute, University of Richmond faculty in biology, chemistry, mathematics, physics, and computer science teamed up to offer first- and second-year students the opportunity to contribute to vibrant, interdisciplinary research projects. The result was not only good science but also good science that motivated and informed course development. Here, we describe four recent undergraduate research projects involving students and faculty in biology, physics, mathematics, and computer science and how each contributed in significant ways to the conception and implementation of our new Integrated Quantitative Science course, a …

Impact Of Interdisciplinary Undergraduate Research In Mathematics And Biology On The Development Of A New Course Integrating Five Stem Disciplines, Lester Caudill, April L. Hill, Kathy Hoke, Ovidiu Z. Lipan

Biology Faculty Publications

Funded by innovative programs at the National Science Foundation and the Howard Hughes Medical Institute, University of Richmond faculty in biology, chemistry, mathematics, physics, and computer science teamed up to offer first- and second-year students the opportunity to contribute to vibrant, interdisciplinary research projects. The result was not only good science but also good science that motivated and informed course development. Here, we describe four recent undergraduate research projects involving students and faculty in biology, physics, mathematics, and computer science and how each contributed in significant ways to the conception and implementation of our new Integrated Quantitative Science course, a …

Nona-Arginine Facilitates Delivery Of Quantum Dots Into Cells Via Multiple Pathways, Yi Xu, Betty Revon Liu, Han Jung Lee, Katie Shannon, Jeffrey G. Winiarz, Tien-Chun Wang, Huey-Jenn Chiang, Yue-Wern Huang

Biological Sciences Faculty Research & Creative Works

Semiconductor quantum dots (QDs) have recently been used to deliver and monitor biomolecules, such as drugs and proteins. However, QDs alone have a low efficiency of transport across the plasma membrane. In order to increase the efficiency, we used synthetic nona-arginine (SR9), a cell-penetrating peptide, to facilitate uptake. We found that SR9 increased the cellular uptake of QDs in a noncovalent binding manner between QDs and SR9. Further, we investigated mechanisms of QD/SR9 cellular internalization. Low temperature and metabolic inhibitors markedly inhibited the uptake of QD/SR9, indicating that internalization is an energy-dependent process. Results from both the pathway inhibitors and …

Production And In Vitro Investigation Of Vitamin B12-Based Bioprobes, Amy Rabideau

Renée Crown University Honors Thesis Projects - All

Vitamin B₁₂ (B₁₂)-based bioprobes were produced and investigated in vitro for studying the B₁₂ uptake pathway in certain cancer cell lines and their target-specific capabilities as imaging and/or contrast agents in vivo. An intrinsic factor (IF)-bound B₁₂ conjugate (B₁₂-ReBQBA) was produced containing rhenium(I) for in vitro analysis of the cubilin-expressing placental choriocarcinoma cell line. B₁₂-ReBQBA has millimolar toxicity in vitro and excitation and emission wavelengths in the visible region, 488 nm and 560 nm, respectively. The rhenium metal in this bioprobe can be readily exchanged for the metastable radioactive metal technetium-99m …

Cadmium, Copper, Iron, And Zinc Concentrations In The Kidneys Of Grey Wolves (Canis Lupus) From Alaska, Idaho, Montana, And The Northwest Territories, Susan Blunck, Emily Jones, Kelly Petersen, Given Harper, Facutly Advisor, Jeffrey Frick, Faculty Advisor

John Wesley Powell Student Research Conference

No abstract provided.

An Entrepreneurial Approach To Librarianship, Flora Shrode, Jennifer Duncan, Wendy Holliday

Library Faculty & Staff Publications

Librarians from Utah State University explain recent efforts to encourage subject librarians to take a more holistic view of their roles. We are shifting from a traditional emphasis primarily on collection development and refocusing on natural connections between collections, instruction, liaison, and reference service. The poster provides background about Utah State University’s situation and explains our approach to analyzing local needs and culture to inform development of a new organizational structure. We describe our vision of subject librarianship, the process by which we assessed librarians’ ideas and goals for performing as subject librarians, and the actions we are taking to …

Volume 03, Cheryl Peck, Charles Hoever, Longwood Theater Department, Brittany Anderson, J. Ervin Sheldon, Richard Hayden, Yuri Calustro, Candice Fleming, Rebecca Franklin, Ashley Yocum, Danielle M. Jagoda, Cristina M. Valdivieso, Jameka Jones, Amy Ellis, Ashley Maser, Erikk Shupp, Jamie Yurasits, Joshua Davis, Alexander Leonhart, Kenny Wolfe, Sally Meadows, J. Haley, Amy Jackson, Morgan Howard, Adrienne Heinbaugh, Melissa Dorton, Ciarra Stalker

Incite: The Journal of Undergraduate Scholarship

Introduction from Dean Dr. Charles Ross

Little Shop of Horrors by Longwood Theater Department

Who Has the Hottest Hotsauce in Farmville: A Quantitative Comparison of Sauces from Local Restaurants by Cheryl Peck and Charles Hoever

Precipitation Effects on the Growth of White Oaks and Virginia Pines on the Mt. Vernon Plantation by Brittany Anderson

Design and Synthesis of Novel Ion Binding Molecules for Self-Assembly and Sensing Applications by J. Ervin Sheldon

A Statistical Analysis of Algorithms for Playing SameGame by Richard Hayden

Intersecting Cylinders at Arbitrary Angles by Yuri Calustro

Putting a Foot in the Revolving Door: Strategies for Reducing …

Inhibition Of Nonsense-Mediated Mrna Decay By Antisense Morpholino Oligonucleotides Restores Functional Expression Of Herg Nonsense And Frameshift Mutations In Long-Qt Syndrome, Qiuming Gong, Matthew R. Stump, Zhengfeng Zhou

Faculty Publications - Department of Biological & Molecular Science

Mutations in the human ether-a-go-go-related gene (hERG) cause long-QT syndrome type 2 (LQT2). We previously described a homozygous LQT2 nonsense mutation Q1070X in which the mutant mRNA is degraded by nonsense-mediated mRNA decay (NMD) leading to a severe clinical phenotype. The degradation of the Q1070X transcript precludes the expression of truncated but functional mutant channels. In the present study, we tested the hypothesis that inhibition of NMD can restore functional expression of LQT2 mutations that are targeted by NMD. We showed that inhibition of NMD by RNA interference-mediated knockdown of UPF1 increased Q1070X mutant channel protein expression and hERG current …

Mutational Studies Uncover Non-Native Structure In The Dimeric Kinetic Intermediate Of The H2a–H2b Heterodimer, Matthew R. Stump, Lisa M. Gloss

Faculty Publications - Department of Biological & Molecular Science

The folding pathway of the histone H2A–H2B heterodimer minimally includes an on-pathway, dimeric, burst-phase intermediate, I2. The partially folded H2A and H2B monomers populated at equilibrium were characterized as potential monomeric kinetic intermediates. Folding kinetics were compared for initiation from isolated, folded monomers and the heterodimer unfolded in 4 M urea. The observed rates were virtually identical above 0.4Murea, exhibiting a log-linear relationship on the final denaturant concentration. Below ∼0.4 M urea (concentrations inaccessible from the 4-M urea unfolded state), a rollover in the rates was observed; this suggests that a component of the I2 ensemble contains non-native structure that …

Measurements Of Platinum Electrode Potential In Blood And Blood Plasma And Serum, Mogely Khubutiya, Anatoly K. Evseev, Vladimir Kolesnikov, Mikhail M. Goldin, Alexei Davydov, Alexander Volkov, Andrey Stepanov

Faculty Publications and Presentations

The method of electrochemical pretreatment of platinum electrode with the goal of standardizing the initial state of electrode surface and its open-circuit potential (OCP) in the blood and other biological media is proposed. The platinum electrode potential is measured in 0.14 M Na2SO4 aqueous solution, in the blood and blood plasma and serum. By the examples of OCP measured in the blood serum of patients with acute poisoning, acute cerebral pathology and patients treated by the method of hyperbaric oxygenation, it was found that the values of blood serum OCP were different for studied pathological states and healthy people.