Biology Commons^™

Genome Editing Using Crispr-Cas9 In Annual Killifish Species, Keria Moritsugu-Vandehey

University Honors Theses

CRISPR-Cas9 genome editing has been used successfully to knock out genes in model organisms such as zebrafish, turquoise killifish, and cichlid fish. CRISPR-Cas9 genome editing has not been verified in the annual killifish, Austrofundulus limnaeus. We hypothesize that targeted editing of the tyrosinase gene in embryos of A. limnaeus will lead to fish without the ability to produce black pigment. Embryos at the 1-cell stage were injected with a Cas9 cocktail containing a mix of guide RNA molecules that target the genomic sequence of the tyrosinase gene and either an mRNA coding for the Cas9 protein or Cas9 protein. …

Physical Properties Of Odorants Affect Behavior Of Trained Detection Dogs During Close-Quarters Searches, Daniel Mejia, Lydia Burnett, Nicholas Hebdon, Peter Stevens, Alexis Shiber, Clay Cranston, Lauryn Degreeff, Lindsay D. Waldrop

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Trained detection dogs have a unique ability to find the sources of target odors in complex fluid environments. How dogs derive information about the source of an odor from an odor plume comprised of odorants with different physical properties, such as diffusivity, is currently unknown. Two volatile chemicals associated with explosive detection, ammonia (NH₃, derived from ammonium nitrate-based explosives) and 2-ethyl-1-hexanol (2E1H, associated with composition C4 plastic explosives) were used to ascertain the effects of the physical properties of odorants on the search behavior and motion of trained dogs. NH₃ has a diffusivity 3.6 times that of …

Exploring Definitions Of Daily Enteric Methane Emission Phenotypes For Genetic Evaluations Using A Population Of Indoor-Fed Multi-Breed Growing Cattle With Feed Intake Data, Clodgah V. Ryan, Thierry Pabiou, Deirdre C. Purfield, Donagh P. Berry, Stephen Conroy, Craig P. Murphy, Ross Evans

Department of Biological Sciences Publications

Genetic selection has been identified as a promising approach for reducing enteric methane (CH₄) emissions; a prerequisite for genetic evaluations; however, these are estimates of the necessary genetic parameters based on a population representative of where the genetic evaluations will be used. The objective of this study was, therefore, to derive genetic parameters for a series of definitions of CH₄, carbon dioxide (CO₂), and dry matter intake (DMI) as well as genetic correlations between CH₄, CO₂, and DMI in a bid to address the paucity of studies involving methane emissions …

Honest Signalling Of Age In A Territorial Breeder, Brian A. Hoover, Walter H. Piper

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Age often indicates phenotypic quality, ecological experience or social status in animals. Consequently, behavioural signals in territorial animals that accurately convey age to potential mates or rivals might provide useful information for both signaller and receiver about the likelihood of aggression. We tested whether male territorial yodels convey information about age in the common loon, Gavia immer. Using a sample of male loons of known age, we found that the dominant frequency of Fintro3, a dominant syllable in the beginning sequence of a male yodel, increases strongly with age, but we were unable to replicate an earlier finding linking …

Effects Of Beauveria Bassiana On The Mortality And Thorax Width Of Bombus Impatiens Colonies, Bri Petty

Undergraduate Research Symposium

Pesticides are widely used to protect against unwanted insects, but risk assessments of the effects on pollinators have traditionally only been applied on an individual level, not a population level. This has allowed many pesticides to be approved without the knowledge of sub-lethal effects, such as life history characteristics and population structure to name a few. The biopesticide BotaniGard, containing the entomopathogenic fungus Beauveria bassiana, has previously been thought to be safe for non-targeted insects, but recent studies have found it to have significant negative effects on pollinators. Such negative effects can include decrease in longevity and odorant responsiveness with …

Parental Environnement Influences Offspring Gene Expression In The Olympia Oyster, Ostrea Lurida., Mark S. Yeats

LSU Master's Theses

The Olympia Oyster is the only native oyster species on the Pacific coast of North America. Seasonality in water temperatures across this threatened species’ broad and fragmented range has been linked to its development, reproductive timing, reproductive activity, growth, and survival rates. Recent evidence suggests parental environments can have carry-over effects on larval growth and respiration rates in response to temperature. In this study, we utilized transcriptomic methods to identify the underlying mechanisms of this cross-generation plasticity. Sequence libraries of adult ctenidia tissue and pooled larval replicates were extracted and spawned from hatchery raised oysters of the same parentage immediately …

Attitudes Of South-Central Kentucky Residents Towards Coyotes And Their Management, Andrew T. Mcqueen, Stephen Sumithran Phd

Posters-at-the-Capitol

The existence of the coyote in the eastern U.S. is a recent development and the public’s perception of coyotes has been widely studied in urban areas. However, perceptions in rural areas, especially rural Appalachia, have been largely unstudied. It is necessary for wildlife managers to understand the public’s perceptions of coyotes and coyote management when creating management plans. We conducted a public survey in South-central Kentucky to determine perceptions toward coyotes and coyote management methods. The survey contained questions about demographics (age, gender, county of residence, landownership, etc.), perceptions (fears, opinions), knowledge (true/false questions), and preferred management practices (hunting, trapping, …

Microhabitat Selection Of Adult Female Eastern Diamondback Rattlesnakes (Crotalus Adamanteus) In The Carolinas, Matthew Gacheny

Theses, Dissertations and Capstones

Reptiles rely on environmental conditions to regulate body temperature using behavioral strategies to meet basic life history needs. Viviparous squamates use thermoregulatory behaviors to meet energetic requirements for vitellogenesis and gestation, which carry considerable energetic costs and must balance with maintenance and survival. Embryonic development is often optimized in a narrower temperature range than the mother’s preferred temperature, creating an intergenerational conflict that should be addressed with maternal microhabitat selection. Field-based studies are needed to understand the link between the thermal biology of reptiles, their reproduction, and implications for population viability. With a use-availability logistic regression framework, I examined thermal …

Factors Associated With Acoustic Bat Presence During Spring Emergence In The Appalachian Mountains Of Western Virginia, Emily Kirk Pody

Theses, Dissertations and Capstones

Studies of threats that bats face during hibernation have increased in response to white-nose syndrome (WNS), a fungal disease that has ravaged North American bat populations. However, impacts of WNS on bat ecology during spring emergence, when bats are potentially recovering from infection and allocating resources for reproduction, is relatively understudied. As more bat species become listed at the federal and state level, the need to understand the factors associated with spring emergence is critical for improving conservation guidelines and habitat management practices. Acoustic monitoring is an efficient method for monitoring bat presence for prolonged periods of time, giving biologists …

Do Nest Box Temperatures Affect Nest Success And Nestlings Growth For Eastern Bluebirds?, Mathew Gordon, Katie Stumpf

Graduate Research Showcase

Rising global temperatures due to climate change have caused declines in many species. For birds, temperature is a primary factor in nest site selection and nest success, so successful management will require temperature mitigation techniques. Secondary cavity nesting birds such as the Eastern Bluebird are particularly vulnerable as common occupants of nest boxes, which are often warmer inside than the outside ambient temperature. Temperatures exceeding 42°C can have numerous negative consequences for Bluebirds, including reducing hatching success and nestling growth. We monitored 50 Eastern Bluebird nest boxes (25 are painted white and 25 are unpainted) at Panola Mountain State Park …

Mark-Recapture Methods In Eastern Diamondback Rattlesnake (Crotalus Adamanteus) Monitoring, Carissa J. Adams

Theses, Dissertations and Capstones

Eastern diamondback rattlesnakes (Crotalus adamanteus; EDB) are an imperiled species native to the Southeastern United States longleaf pine (Pinus palustris) ecosystem. Several markrecapture methods have been used range-wide in hopes of increasing knowledge on their population numbers. I explored the efficacy of two mark-recapture methods (scale cauterization and dorsal head photography) using the Marine Corps Recruit Depot, Parris Island, SC EDB population in this multi-part study. The first method I evaluated was ventral scale branding, which has been widely implemented without knowledge of brand permanence. I classified misidentification errors at capture status (new versus recapture) and …

Patterns Of Genetic Structure In Spiders Beetles, Naiya Sims

Mahurin Honors College Capstone Experience/Thesis Projects

Spider beetles are a poorly known group of North American beetles in the family Ptinidae. Protection of biodiversity requires knowledge of species- and population-level distinctiveness, so data regarding genetic structure of spider beetles can shed light on conservation priorities. Spider beetles in the genus, Coleotestudus, are distributed broadly in the southwestern US. Given their small size and lack of flight, it is presumed that the dispersal capability of Coleotestudus is limited, leading to strong population structure on a small spatial scale. We used a three-enzyme restriction-site-associated DNA sequencing (3RAD) strategy to identify single nucleotide polymorphisms (SNPs) throughout the genome …

Environmental Biology Masters Capstone, Antonio Gonzalez-Pita

Regis University Student Publications (comprehensive collection)

Human wildlife interactions (HWI) pose a complex challenge for wildlife managers. Human encroachment into wildlife habitat and the growing number of outdoor recreationists are increasing the frequency of contact and conflict, especially in regions such as the Front Range of Colorado. Geographic information systems (GIS), which use a combination of remote sensing and environmental survey data, allow for predictive spatial analyses of where human wildlife interactions are likely to occur. I used publicly reported observations of moose to create spatial predictive maps in a species distribution model framework. Slope and elevation were shown to be the strongest predictors of HWI, …

The Effects Of Habitat Characteristics And Location On Brook Trout Size Distribution, Bryce Groves

Journal of Earth and Life Science

Adfluvial Brook Trout Salvelinus fontinalis are known to typically grow larger than riverine Brook Trout. The Kabekona River in Hubbard County, Minnesota exhibits suitable habitat for both fluvial and adfluvial Brook Trout populations. The objective of this study was to analyze the relationships between depth, stream width, canopy cover, water temperature, sediment size, and distance from Kabekona Lake on Brook Trout size distribution in the Kabekona River. Brook Trout (n=35) were angled from the Kabekona River from August 24 to September 26, 2023. Each fish was measured and released, with habitat metrics being recorded at time of release. ArcGIS was …

How Does A Global Climate Cycle Affect The Reproductive Output Of A Neotropical Migratory Bird?, Abby K. Bressette, Emma Cummings, Daniel Albrecht-Mallinger, Lesley Bulluck

UROP Posters

El-Niño Southern Oscillation (ENSO) causes regional changes in climate and has been found to have varying effects on the survival, fecundity, and recruitment of migratory songbirds. This study seeks to better understand the effect of ENSO on the fecundity of Virginia-breeding Prothonotary warbler (Protonotaria citrea; PROW), a neotropical migratory songbird. PROW breed in the Eastern United States and spend the non-breeding season primarily in Panama and Colombia. Ongoing research in Virginia has found a positive relationship between spring precipitation and predicted number of PROW fledgelings during La Niña, but the opposite during El Niño. To better understand this relationship, we …

Carry-Over Effects Of Climate On Prothonotary Warbler (Protonotaria Citrea) Feather Quality, Alyssa N. Spasic, Dan Albrecht-Mallinger, Lesley Bulluck

UROP Posters

Avian species have inspired numerous studies on ornamentation, exploring its role in intra- and intersexual selection as a marker for individual quality. Male birds have been the focus of most of these studies while females, which can also display ornamentation, have been studied less. Coloration achieved through molt, particularly carotenoid-based pigments, requires energy derived from food resources. As climate affects insect abundance, there may be a link between climate conditions during molt and feather quality the following season. We assessed the relationship between temperature and precipitation during molt and female Prothonotary Warbler (Protonotaria citrea) breast and crown feather reflectance metrics …

Description Of Neoperla Philippinensis Sp. Nov. Confirmed Using Integrative Taxonomy And Some Notes On Neoperla Oculata Banks, 1924 (Plecoptera: Perlidae), Arthien Lovell P. Pelingen

Biology Faculty Publications

I re-examine the assumed phenotypic variable Neoperla oculata Banks, 1924 (Plecoptera, Perlidae) and describe Neoperla philippinensis sp. nov. All material examined herein are collections from the Mindanao region, of which supplementary information were provided including COI mtDNA accessions, female adult associations, and egg morphology characterization.

Incorporating Sex-Diverse And Gender-Inclusive Perspectives In Higher Education Biology Courses, Breanne N. Harris, A Kelsey Lewis, Sam L. Sharpe, Teri J. Orr, Christopher T. Martine, Chloe C. Josefson

Faculty Journal Articles

Inclusive teaching is teaching in a way that reaches all students in the classroom; this is beneficial for everyone, particularly for those with minoritized identities. Instructors play a critical role in scaffolding how students are exposed to and learn science content in the classroom. In this manuscript, we discuss how biology instructors can make their classrooms more inclusive with regard to sex and gender diversity content. Many topics in biology are based on androcentric, heteronormative, and oppressive framing, even though those lenses are more reflective of our own history and culture than they are of the diversity we see in …

Osteohistological Assessment Of Edmontosaurus Radii From The Liscomb Bonebed, Prince Creek Formation, North Slope, Alaska, Emma Griffin

Student Research Poster Presentations 2024

Hadrosaurs were one of the most diverse dinosaur groups in the Late Cretaceous period and are known for their numerous articulated skeletons and monodominant bonebeds (Wosik et al., 2020). They are more commonly known as duck-billed dinosaurs and are found globally including latitudinal extremes such as the artic and Antarctica. Edmontosaurus, a genus of hadrosaur, are herbivores and are very large growing up to 8,000 pounds. Edmontosaurus were known to travel in herds as a defense mechanism and were one of the last non-avian dinosaurs living during the late Campanian stage through the end of the Maastrichtian stage, right up …

Effect Of Walleye Fry Stocking On Future Gillnet Cpue, Alexander Barker

Journal of Earth and Life Science

Walleye Sanders vitreus for many states, is a key attractant of anglers from around North America. Due to immense popularity, management efforts have been conducted to maintain Walleye populations, especially in the form of stocking. The purpose of this study is to analyze the effect of Walleye fry stocking on gillnet catch per unit effort (CPUE). A total of 90 lakes were analyzed for this study within three defined regions of Minnesota, 30 from each region. The regions chosen for this study are the Minnesota Department of Natural Resources northern pike Esox lucius regulation regions which consist of the Southern, …

Feeding Systems And Fresh Lamb Color Stability, Koushik Mondal

Theses and Dissertations--Animal and Food Sciences

Consumers consider the surface color of fresh lamb as the indicator of freshness and wholesomeness, which affects their purchase decisions. The redox chemistry of myoglobin (Mb) determines the color of fresh lamb. Artificial raising on milk replacer (a pre-weaning strategy) as well as red clover supplementation are management strategies used in lamb production. This thesis focuses on the effects of artificial raising on milk replacer and red clover supplementation on the color and oxidative stability of fresh lamb.

In the first experiment, the effect of pre-weaning with milk replacer on the carcass attributes, color stability and lipid oxidation of lamb …

The Morphological Evolution Of The Inner Ear In Lacertoidea (Squamata), Cristian Hernandez

Biology Dissertations - Archive

The popularization of high-resolution computed tomography(HRCT) has produced a revolution in the field of comparative anatomy. This technology allows us to reach a level of morphological detail in our observations that has never been possible previously. Now we can perform digital dissections isolating structures of interest such as individual bones, soft tissue organs, and even generate 3D models of body cavities (endocasts) such as the braincase and inner ear. Encompassed with the progress of the HRCT, 3D geometric morphometrics have emerged as a common tool to address morphological questions in a statistical context that outperforms the traditional 2D geometric morphometrics. …

Estimation Of Probability Of Habitat Use Of Roosevelt Elk On The Olympic Peninsula, Vincent Michael Gugliotti

Undergraduate Theses, Professional Papers, and Capstone Artifacts

Estimating the probability of habitat use for a particular species is crucial to the direct management and conservation of that species. Without knowledge of habitat preferences, managers cannot effectively focus efforts on vital resources or landscape types. However, modelling probability of habitat use can be done in several ways which leaves room for variation and uncertainty in the estimates produced by each method. This study is an examination of the variation between two estimates of probability of habitat use while focusing on a particular subspecies of elk that inhabits a unique ecosystem relative to other elk subspecies. I modeled elk …

Fillet Weight Comparison For Bluegill, Black Crappie, And Yellow Perch, Mikenna A. Pattrin

Journal of Earth and Life Science

Panfish, including bluegill Lepomis macrochirus, black crappie Pomoxis nigromaculatus, and yellow perch Perca flavescens are three sought-after species. Anglers often perceive a change in regulations as an attempt at limiting the amount of harvest. However, the goal of many regulation proposals is to increase the size structure of panfish, allowing anglers to achieve their ideal harvest with less fish. The primary objective of this study was to assess the relationship between panfish fillet weight and total length to estimate how many fillets would be needed to reach a harvest weight of 0.5 lbs. The secondary objective was to …

Specific Measurements Of A Yellow Perch Head Compared To The Total Length, Alex Timm

Journal of Earth and Life Science

Yellow Perch Perca flavescens is a popular fish for harvesting in Minnesota. Because of this the Minnesota Department of Natural Resources survey populations with gill nets, however, some water bodies have Rusty Crayfish Faxonius rusticus that commonly consume most of the body leaving only the head to be accurately measured. The goal of this research was to see if there is a relationship between specific measurements of the head and the total length of the fish. The number of Yellow Perch sampled in this study was 72 and all came from Lake Bemidji in Minnesota during the month of October …

Nocturnal Behavior Of Communally Roosting American Crows (Corvus Brachyrhynchos), Caitlin M. Mrowiec

Masters Theses

During the winter months in Charleston, Illinois, thousands of American crows (Corvus brachyrhynchos) gather together each night to roost. While aspects of this impressive sight have been studied elsewhere, there is very limited information on how crows behave at night. Additionally, the mechanics and origins of communal roosting are not especially well understood, and the behaviors involved are not always consistently described. This study sought to provide a more complete assessment of crow communal roosting after sunset. I collected data between 2018 and 2020 by locating communal roosts and conducting continuous observations for 4 hours after sunset, with additional checks …

Softening And Cross-Susceptibility: Exposure To Heat And Desiccation Reduces Future Stress Tolerance In An Insect, Lauren Harter

University of the Pacific Theses and Dissertations

The frequency and duration of environmental stressors, such as heat waves and drought, will continue to grow due to ongoing climate change, thereby increasing the likelihood that organisms will experience stressors consecutively. Exposure to one stressor can improve or impair future tolerance to the same stressor (i.e., hardening or softening, respectively), or enhance or reduce future tolerance to a different stressor (i.e., cross-protection or cross-susceptibility, respectively). Understanding whether stress improves or impairs animals’ abilities to withstand future stressors is critical for determining the physiological sensitivity of animals to ongoing climate change. Here, I evaluated the responses to consecutive heat and …

Behavioral Ecology Of The Colorado Brown Tarantula (Aphonopelma Hentzi): Activities Displayed Around The Burrow Through Juvenile Group Dispersal, Bradley Allendorfer

Master's Theses and Doctoral Dissertations

The life history strategy of the Colorado brown tarantula (Aphonopelma hentzi) typically includes males spatially scattering in search for a female mate in the fall, followed by female attendance of fertilized eggs the following summer. Here, I highlighted aspects associated with this female parental care. Specifically, I compared the activities of maternal (egg-attending) tarantulas to their non-maternal (not egg-attending) counterparts, as this may be a costly time for these egg-attending individuals. I concluded that maternal individuals tended to show reduced activity at the burrow entrance, however; these, results were significant in only one comparison. After weeks of this parental care, …

Occupancy Of The Little Grass Frog (Pseudacris Ocularis), An Overlooked Longleaf Endemic, In A Managed Landscape, Anna M. Humphrey

Theses, Dissertations and Capstones

Amphibians are declining globally, with many declines associated with habitat loss. The longleaf pine (Pinus palustris) ecosystem historically covered most of the southeastern United States and supports a host of endemic amphibians. This ecosystem has declined by over 97% in the last two hundred years and many associated species have declined with it. The Little Grass Frog (Pseudacris ocularis) is a longleaf pine endemic anuran that has been understudied and under-surveyed; almost no information is known on the ecology of this species and no population studies have been conducted. My goals were to create an acoustic …

Relationships Between Cliff Swallows (Petrochelidon Pyrrhonota) And Feather Coloration, Morphological Characteristics, And Colony Size, Sonja M. Brandt

Master's Theses or Doctor of Nursing Practice

Environmental stressors such as drought and roadways can strongly affect breeding birds. Cliff Swallows (Petrochelidon Pyrrhonota) are aerial insectivores that experience mortality from car collisions, because they often nest and hunt under bridges and road culverts. Individual traits that may convey social information, such as body condition, or feather coloration, might be associated with better coping around cars and other environmental stressors. Few studies have investigated relationships between coloration and other environmental stressors, such as water loss due to drought near the colony and foraging areas. Colony size can be heavily affected by various environmental factors such as water level …