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Full-Text Articles in Biochemistry
Structural Studies Of Membrane-Assembled Popd And Popb, The Pseudomonas Aeruginosa Type 3 Secretion Translocators, Fabian B Romano Chernac
Structural Studies Of Membrane-Assembled Popd And Popb, The Pseudomonas Aeruginosa Type 3 Secretion Translocators, Fabian B Romano Chernac
Doctoral Dissertations 1896 - February 2014
Transport of proteins across membranes is essential during many stages of pathogen infection and colonization of human cells. Many Gram-negative pathogens use a Type 3 Secretion (T3S) system to inject proteins into the target cell during infection. Substantial genetic and biochemical evidence suggest that proteins are translocated across the host plasma membrane through a proteinaceous pore or translocon formed by two bacterial secreted proteins: the T3S translocators. Despite its key role in pathogenesis, virtually nothing is known about the assembly mechanism, structure, and composition of this critical transmembrane complex.
To this end, a cell-free system for the structural and functional …