Biochemistry Commons^™

Perseverance Of Protein Homeostasis Despite Mistranslation, Farah Hasan

Electronic Thesis and Dissertation Repository

Transfer RNAs (tRNAs) are essential for protein synthesis and translation fidelity. Some human tRNA variants may cause amino acid misincorporation: tRNA^Gly variants (tRNA^Gly_CCC, tRNA^Gly_GCC) have mutations that generate an alanine tRNA identity element (G3:U70), likely causing mis-aminoacylation of glycine tRNAs with alanine, while the tRNA^Ala_AGC G35C (tRNA^Ala_ACC) variant may function similarly to mis-incorporate Ala at Gly codons by generating a Gly anticodon. I propose that these mistranslating tRNAs will disrupt protein homeostasis in mammalian cells. Although the tRNA^Gly and tRNA^Ala variants did not affect protein synthesis …