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Full-Text Articles in Biochemistry
Primer Efficacy In The Dna Barcoding Of Spiders, Rhennetta Jo Bork
Primer Efficacy In The Dna Barcoding Of Spiders, Rhennetta Jo Bork
Honors Program Theses
DNA barcoding is the process of amplifying a 650 base pair segment of the sequence of the mitochondrial gene cytochrome c oxidase (COI), and amplifying this gene with a polymerase chain reaction (PCR). It is used to help identify and distinguish animal species and also to help determine genetic differences in species. DNA barcoding can be especially useful when working with spiders since they tend to be very small and hard to distinguish. However, achieving a DNA barcode can be difficult and thus techniques to improve the method of DNA barcoding can be helpful. This research looked into the different …
Non-Destructive Dna Extraction Methods That Yield Dna Barcodes In Spiders, Reva Ann Bork
Non-Destructive Dna Extraction Methods That Yield Dna Barcodes In Spiders, Reva Ann Bork
Honors Program Theses
DNA barcodes are short sequences of nucleotides that differ from species to species (Hebert et al., 2003; Hebert & Gregory, 2005). DNA barcoding is very important in helping to reconstruct phylogenetic trees and to confirm the identity of threatened or endangered species in the wild. Additionally, it has become increasingly popular in the food industry to test the accuracy of the food being sold in fast food chains (Wong and Hanner, 2008). For scientific purposes, nondestructive DNA extraction techniques need to be explored because they allow for preservation of the voucher specimen, which is particularly important for rare or extinct …