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Articles 1 - 7 of 7
Full-Text Articles in Biochemistry
Critical Amino Acids Of The Gα2 Subunit Helical Domain In Dictyostelium Discoideum, Steven Martin Rauch
Critical Amino Acids Of The Gα2 Subunit Helical Domain In Dictyostelium Discoideum, Steven Martin Rauch
Electronic Theses and Dissertations
In Dictyostelium discoideum organism, the Gα2 subunit of the heterotrimeric G-protein signaling complex plays a pivotal role during the aggregation stage in the Dictyostelium life cycle. The biochemical functions of the G-protein complex include separation of the G-protein coupled receptor from the G-protein subunits, GDP displacement by GTP in the Gα subunit, separation of the Gα monomer from the βγ complex, GTP hydrolysis to GDP, activation of adenylyl cyclase as a downstream effector, and activation of guanylyl cyclase as a separate downstream effector. Upon release from the heterotrimer, the βγ subunits lead to downstream activation of the membrane bound adenylyl …
Analysis Of Protistan Grazing On Bioremediative Bacteria Using In Wvo Fluorescent Protein Expression And Flow Cytometry, Yutao Fu
Electronic Theses and Dissertations
Protistan bacterivory can influence the size distribution, cell structure and composition of natural bacterial communities and is of significant concern for design of bioremediation efforts, yet adequate methods for observation and modeling are lacking. In this investigation, fluorescent protein expression and flow cytometry were used to study protistan grazing on genetically modified strains of several bacterial species that have been considered for use in bioremediation. Broad-host-range plasmids were constructed and used to introduce genes encoding GFP (green fluorescent protein) or RFP (red fluorescent protein) to prey species. A heterotrophic flagellate Paraphysomonas imperforata (Hflag) served as a model predator. Predator-prey interactions …
Investigation Of Biochemical Mechanisms Associated With Insulin Resistance In The Non-Insulin-Dependent Diabetes Mellitus, To-Yu Huang
Doctoral Dissertations
Non-insulin dependent diabetes mellitus (NIDDM) is a heterogeneous disease arising from metabolic defects in insulin secretion and insulin action. The inability of insulin to trigger full metabolic responses results in impaired glucose transport, which elicits compensatory hyperinsulinemia with increased lipogenesis. In order to identify the molecular defects responsible for insulin resistance, we have investigated three different model systems: the ability of sulfonylurea receptor (SUR) ligands to modify insulin regulated events in cultured rodent adipocytes; the ability of agouti and/or agouti related polypeptide (AGRP) to modify insulin regulated events; and the regulation of the activity and amount of the HNF-1a transcription …
The Modulation And Cross-Talk Between Stat 5 And Socs-3 In Insulin And Growth Hormone Signaling In 3t3-L1 Adipocytes., David James Story
The Modulation And Cross-Talk Between Stat 5 And Socs-3 In Insulin And Growth Hormone Signaling In 3t3-L1 Adipocytes., David James Story
Honors Theses
No abstract provided.
Production Of A Monoclonal Antibody Against Benzo[Α]Pyrene Diol Epoxide Dna Adducts, Brian Peden Austin
Production Of A Monoclonal Antibody Against Benzo[Α]Pyrene Diol Epoxide Dna Adducts, Brian Peden Austin
Chemistry & Biochemistry Theses & Dissertations
Benzo[α]pyrene is a ubiquitous pollutant produced from the incomplete combustion of organic material such as fossil fuels. It is found in the workplace, urban air, drinking water, and the food supply. Recently, it has been proposed that benzo[α]pyrene may be the causative agent in the formation of lung adenocarcinomas among some Taiwanese women exposed to cooking oil fumes without adequate ventilation. In this study, calf thymus DNA was modified in vitro with benzo[α]pyrene-diol epoxide (BPDE) to a level consistent with that found in biological samples. This DNA of low modification was used as an immunogen in the production of a …
A Dynamical Perspective On Enzymatic Catalysis, Shiying Shang
A Dynamical Perspective On Enzymatic Catalysis, Shiying Shang
Dissertations, Theses, and Masters Projects
No abstract provided.
Development Of A Chromatographic Method For The Analysis Of Carbohydrates In Posidonia Coriacea In Artificial Cultures, Olivia J. Oldfield
Development Of A Chromatographic Method For The Analysis Of Carbohydrates In Posidonia Coriacea In Artificial Cultures, Olivia J. Oldfield
Theses : Honours
Carbohydrates in P. coriacea leaves were analysed to determine if, when in artificial culture, they were unable to replenish their carbohydrate stores once the seed starch reserve was consumed. While no evidence was found to suggest that this was happening, other findings were made. Soluble carbohydrates determined in P. coriacea were sucrose, fructose, glucose, trehalose, myo-inositol and mannitol. However, mannitol was only found in in situ plants sampled in June. This may be due to high epiphyte coverage elevating plant stress and therefore mannitol levels. P. coriacea grown in tissue culture has soluble carbohydrate levels up to 20-fold higher than …