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Full-Text Articles in Biochemistry
A Proximity-Dependent Biotin Labeling Based Screen For Protein Kinase A Anchoring Proteins Within Focal Adhesion Complexes, Hannah Naughton
A Proximity-Dependent Biotin Labeling Based Screen For Protein Kinase A Anchoring Proteins Within Focal Adhesion Complexes, Hannah Naughton
Graduate College Dissertations and Theses
Protein kinase A (PKA) regulates a diverse array of cellular activities including metabolism, differentiation, actomyosin contractility, and migration. The multifunctionality of this ubiquitous enzyme is achieved, in part, through subcellular targeting mediated by the A Kinase Anchoring Proteins (AKAP) family of proteins. AKAPs serve as scaffolding proteins that localize PKA to various cellular compartments and bring together specific targets and modulators of PKA activity.
The importance of spatially restricted PKA signaling is particularly apparent in the context of cell motility. It has been observed that both anchoring through AKAPs and the subsequent localized activation of PKA at the leading edge …
Novel Mechanisms Governing Autoregulation Of The Src Family Kinase Fyn And Its Crosstalk With Protein Kinase A, Marion Weir
Novel Mechanisms Governing Autoregulation Of The Src Family Kinase Fyn And Its Crosstalk With Protein Kinase A, Marion Weir
Graduate College Dissertations and Theses
ABSTRACT
Phosphorylation is a post-translational modification important for regulating protein activity and protein binding capacity. It is used in many different signaling pathways within the cell. Src Family Kinases and Protein Kinase A (PKA) are two prototyptical non-receptor tyrosine and serine/ threonine kinases, respectively, which are found in canonical signaling pathways. These two kinases are critical for signaling in essentially every cell of a multicellular organism, and are particularly important in development, cell migration and proliferation. Although both proteins have been intensely studied for many decades, an understanding of the molecular mechanisms which govern their regulation and the regulation that …