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Full-Text Articles in Biochemistry
Intracellular Protein Degradation In A Mesophile And A Thermophile Of The Genus Bacillus, Cameron C. Hoover
Intracellular Protein Degradation In A Mesophile And A Thermophile Of The Genus Bacillus, Cameron C. Hoover
Masters Theses
14C-leucine was incorporated into th e proteins of the mesophile Bacillus licheniformis and the thermophile Bacillus stearothermophilus. Aliquots of the cell suspension were treated with chloramine-T in order to decarboxylate the 14C-leucine that was released as the proteins degraded. The 14CO2 was trapped and counted. This allowed for the determination of the extent of intracellular protein degradation at various tem peratures and at various stages of the growth cun/e. For the mesophile, the extent of degradation increased with temperature from 30° C to 55° C. For the thermophile, no significant amount of degradation occurred until …
Production Of Thymine Dimer In Dna From Mesophilic And Thermophilic Bacteria By Uv Irradiation, F Redrick Shu-Chung Yein
Production Of Thymine Dimer In Dna From Mesophilic And Thermophilic Bacteria By Uv Irradiation, F Redrick Shu-Chung Yein
Dissertations
The in vitro production of thymine dimer (TT) in DNA, isolated from the mesophile (B. licheniformis) and the thermophile (B. stearothermophilus), was carried out by UV irradiation (254 nm) at three different temperatures (35, 45, and 55(DEGREES)C). The production of TT from the thermophilic DNA was significantly higher than that from the mesophilic DNA. This was the case at all three irradiation temperatures. The increase in TT production per 10(DEGREES)C increase in irradiation temperature was four times higher for the thermophile than that for the mesophile. It was concluded that the DNA from the thermophile was photochemically less stable than …