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Articles 1 - 3 of 3
Full-Text Articles in Biochemistry
Iron-Sulfur Cluster Assembly; In Vivo Analysis Of The Methanogenic Suf System, Evan Dunkle
Iron-Sulfur Cluster Assembly; In Vivo Analysis Of The Methanogenic Suf System, Evan Dunkle
LSU Master's Theses
Iron-sulfur (Fe-S) clusters are among the most ancient and prevalent of all biological cofactors. Their assembly into associated proteins is a tightly regulated process with many organisms employing multiple cluster assembly pathways. Much is known about Fe-S cluster assembly in aerobic organisms such as Escherichia coli (E. coli) but little is known in regards to cluster assembly in more ancient organisms such as methanogens. Methanogens are members of the domain of Archaea and are defined by their ability to generate methane as a byproduct of their main energy generating pathway. Methanogens also have significantly higher Fe-S cluster content …
Maximizing And Modeling Malonyl-Coa Production In Escherichia Coli, Tatiana Thompson Silveira Mello
Maximizing And Modeling Malonyl-Coa Production In Escherichia Coli, Tatiana Thompson Silveira Mello
LSU Master's Theses
In E. coli, fatty acid synthesis is catalyzed by the enzyme acetyl-CoA carboxylase (ACC), which converts acetyl-CoA into malonyl-CoA. Malonyl-CoA is a major building block for numerous of bioproducts. Multiple parameters regulate the homeostatic cellular concentration of malonyl-CoA, keeping it at a very low level. Understanding how these parameters affect the bacterial production of malonyl-CoA is fundamental to maximizing it and its bioproducts. To this end, competing pathways consuming malonyl-CoA can be eliminated, and optimal nutritional and environmental conditions can be provided to the fermentation broth. Most previous studies utilized genetic modifications, expensive consumables, and high-cost quantification methods, making …
Environmental Controls On Dissolved Carbon Export And River Geochemistry - A Case Study In The Mississippi-Atchafalaya System, Jeremy Reiman
Environmental Controls On Dissolved Carbon Export And River Geochemistry - A Case Study In The Mississippi-Atchafalaya System, Jeremy Reiman
LSU Master's Theses
Rivers serve as an important medium for the exchange of elements between land, ocean, and atmosphere. This thesis consists of three interconnected studies with the overarching goal of analyzing the environmental factors influencing dissolved carbon dynamics and river geochemistry in large rivers. These studies utilized river water samples and in-stream measurements collected from the Mississippi and Atchafalaya Rivers at hourly to monthly intervals between 2013 to 2018, along with ambient river and meteorological data downloaded from public-access databases. Results indicate substantially higher dissolved organic carbon concentrations (DOC, 611 ±181 µmol L-1) but lower concentrations of dissolved inorganic carbon …