Open Access. Powered by Scholars. Published by Universities.®
- Discipline
- Keyword
-
- Aggregation (1)
- CARBON QUANTUM DOTS (1)
- CFP-10 (1)
- CMT2F (1)
- Chaperone (1)
-
- DHMNIIB (1)
- DRUG DELIVERY (1)
- ESAT-6 (1)
- EsxA (1)
- EsxA:EsxB (1)
- Giardia lamblia (1)
- Hsp27 (1)
- Lipidomics (1)
- Liposome (1)
- Liposomes (1)
- Mass Spectrometry (1)
- Membrane permeabilizing activity (1)
- Metabolism (1)
- Mycpbacterium tuberculosis (1)
- NEURODEGENERATIVE DISORDERS (1)
- OXIDATIVE STRESS (1)
- PESTICIDES (1)
- PROTEIN AGGREGATION (1)
- Parasitology (1)
- Protein (1)
- Proteomics (1)
Articles 1 - 6 of 6
Full-Text Articles in Biochemistry
Development Of A Ph-Triggered Cargo Delivery System With Esxa Mutants, Shuchen Chen
Development Of A Ph-Triggered Cargo Delivery System With Esxa Mutants, Shuchen Chen
Open Access Theses & Dissertations
Tuberculosis (TB) is a communicable disease caused by Mycobacterium tuberculosis (Mtb). It was the leading cause of human death generated by a single infectious agent before the Coronavirus (COVID-19) pandemic. Mtb has a special mechanism to evade the hostâ??s immune system and utilize macrophages as reservoirs. Latent TB infections have been a threat to public health. Liposome has been widely used for drug delivery. Immunoliposomes are special liposome carriers coated with ligands, such as antigens, targeting certain cells or organs to regulate the uptake of liposome. By constructing the liposome components, or surface modification, cargo release of liposome can be …
Eluication Of Lipid Metabolic Pathways In Differentiating Giardia Lamblia Using High Resolution Mass Spectrometry, Cameron Ellis
Eluication Of Lipid Metabolic Pathways In Differentiating Giardia Lamblia Using High Resolution Mass Spectrometry, Cameron Ellis
Open Access Theses & Dissertations
Giardia lamblia is an intestinal protozoan found worldwide, including the U.S. This parasite exists in two morphologic stages - a replicative trophozoite and a relatively dormant yet viable cyst. While exposures of cysts to gastric acid during passage through the human stomach induces excystation, factors in the small intestine, where trophozoites colonize trigger encystation or cyst formation. Transformation into cyst stage is essential for Giardia to survive in the environment for months before infecting new hosts. Because of its small genome size (11.7 Mb), metabolic pathways in Giardia are highly reduced. As far as lipid metabolism is concerned, only limited …
Characterization Of Esxa/Esxb Heterodimer Membrane Interaction With A Novel Popc/Popg Liposome Model, Salvador Vazquez Vazquez Reyes
Characterization Of Esxa/Esxb Heterodimer Membrane Interaction With A Novel Popc/Popg Liposome Model, Salvador Vazquez Vazquez Reyes
Open Access Theses & Dissertations
EsxA and EsxB are co-secreted as a heterodimer and have been shown to play critical roles in phagosome rupture and translocation of Mycobacterium tuberculosis into the cytosol. Current in vitro studies support a model in which the EsxA:EsxB heterodimer is dissociated upon acidification, which might allow EsxA insertion into liposome membranes. While the membrane permeabilizing activity (MPA) of EsxA has been well characterized in liposomes composed of di-oleoyl-phosphatidylcholine (DOPC), the MPA of EsxA:EsxB heterodimer has not been detected with the DOPC liposome. In this study, we established a new in vitro membrane assay to test the MPA of EsxA and …
Nanotechnology-Based Approaches To Mitigate Environmental Pollutants -- Induced Neurological Disorders, Jyoti Ahlawat
Nanotechnology-Based Approaches To Mitigate Environmental Pollutants -- Induced Neurological Disorders, Jyoti Ahlawat
Open Access Theses & Dissertations
Neurodegenerative diseases such as Parkinson's disease (PD), Alzheimerâ??s Disease (AD) and Huntingtonâ??s Disease (HD) have no cure despite intensive research efforts geared towards therapeutics development. In the USA, patients with PD and AD alone cost the nation ~ $200 billion annually in patient care and lost productivity. Therefore, there remains an urgent and unmet need to develop novel drugs and drug carriers with the potential to slow down, halt and ideally reverse the course of neurodegenerative disorders. The dissertation focuses on developing approaches towards targeting PD using multifactorial mechanisms including the targeting of protein aggregation and oxidative stress. This is …
Small Heat Shock Protein 27 And Its Role In Human Disease, Bianka Andrea Holguin
Small Heat Shock Protein 27 And Its Role In Human Disease, Bianka Andrea Holguin
Open Access Theses & Dissertations
Small heat shock protein 27 (Hsp27) is a ubiquitously expressed molecular chaperone with roles in many physiological processes. As an ATP-independent molecular chaperone, Hsp27 protects substrates from irreversible aggregation and holds them in a folding competent state for later recycling into the proteome. Hsp27 proteins form dimers that are assembled into large oligomeric complexes. Phosphorylation of Hsp27 dissembles the oligomers into chaperone active dimers. Several missense mutations of Hsp27 are causative for the neurodegenerative disorders Charcot-Marie-Tooth disease 2F and distal Hereditary Motor Neuropathy IIB. Here I show that the oligomerization and chaperoning ability of Hsp27 are altered by the Hsp27 …
Structural And Biochemical Studies On Disease Associated Proteins: Heat Shock Protein 60 And Hiv Capsid, Alejandro Rodriguez
Structural And Biochemical Studies On Disease Associated Proteins: Heat Shock Protein 60 And Hiv Capsid, Alejandro Rodriguez
Open Access Theses & Dissertations
Structural studies on proteins provide valuable knowledge, from information about their fundamental nature, to laying a foundation for rational drug design. Here, we study two proteins, a human mitochondrial chaperonin heat shock protein 60 (Hsp60) and the main structural HIV-1 protein capsid (CA). We focus on the structural nature of both proteins to try to understand their role in disease and elucidate the mechanism of potential drug treatments. Hsp60 is a chaperonin found in human mitochondria that is responsible for assisting client proteins reach their native, functional, state. The mechanism by which it catalyzes protein folding, however, has not been …