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Full-Text Articles in Biochemistry
Structural Studies Of Scallop Abductin, A Compressible Elastomer That Translates Hydrophobic Interactions To Elasticity, Felipe Jimenez Jr.
Structural Studies Of Scallop Abductin, A Compressible Elastomer That Translates Hydrophobic Interactions To Elasticity, Felipe Jimenez Jr.
Loma Linda University Electronic Theses, Dissertations & Projects
Abductin is a natural elastomer found as the major component of the elastic hinge ligament in marine scallops. Until now, very little was known about the structure and function of this compressible elastomer. Biochemical studies of a synthetic 25 amino acid peptide of abductin produces fibers that assemble into a gel matrix at room temperature. Further investigation of this phenomenon via temperature-dependent turbidimetry revealed that this peptide self-assembles or aggregates at 23 °C. Secondary structural studies of this peptide at different temperatures revealed that it undergoes an inverse temperature transition much like soluble elastin peptides. Replacing phenylalanine with alanine in …
Plant-Based Multicomponent Vaccines Against Enteric Diseases, Jie Yu
Plant-Based Multicomponent Vaccines Against Enteric Diseases, Jie Yu
Loma Linda University Electronic Theses, Dissertations & Projects
To generate a plant-based multicomponent oral vaccine against cholera, rotavirus and enterotoxigenic E.coli (ETEC), we have constructed transgenic potato plants that synthesize the cholera toxin B and A2 subunits, the rotavirus enterotoxin NSP4 22 amino acid epitope and the ETEC fimbrial antigen CFA/L To use cholera toxin as a carrier molecule for delivery of multiple antigens to the gut-associated lymphoid tissues, the rotavirus NSP4 epitope was linked to the cholera toxin B subunit (CTB) and the ETEC fimbrial antigen CFA/I was linked to the cholera toxin A2 subunit. Cholera toxin-antigen fusion proteins were detected in transformed tuber tissues by immunoblot …