Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 2 of 2
Full-Text Articles in Biochemistry
The Role Of The Voltage Gradient In The Agarose Gel Electrophoresis Of Dna, David Wheeler
The Role Of The Voltage Gradient In The Agarose Gel Electrophoresis Of Dna, David Wheeler
Theses and Dissertations in Biomedical Sciences
In Part I of this dissertation, empirical equations for predicting DNA mobility during agarose gel electrophoresis (AGE) from voltage gradient are developed from the data of McDonnel (36) for electrophoresis in a 1.6% agarose gel. These equations represented the data well for DNA between 2 and 10 kilobase pairs (KBp) in length. A computer program, called GELSIM, which incorporates these equations is described in Part II. GELSIM was designed to allow researchers to analyze electrophoresis data by predicting the effect on DNA migration of altering the voltage of electrophoresis. In this way, electrophoretic banding patterns produced using different voltages could …
Molecular Study Of The B19 (Human) Pathogenic Parvovirus, Jamshed Ayub
Molecular Study Of The B19 (Human) Pathogenic Parvovirus, Jamshed Ayub
Theses and Dissertations in Biomedical Sciences
The B19 (human) parvovirus is a small single stranded DNA virus of 5.4 kilobases. B19 is specific for erythroid progenitor cells and has been propagated in vitro only with human erythroid bone marrow. Replication of viral DNA and the viral protein products of B19 appear similar to those of other animal parvoviruses. However, B19 differs from other parvoviruses in some important aspects, which include the initiation of all transcripts at a strong left side promoter (p6) and the absence of a functional internal promoter. B19 has an unusual transcription map which is described in this study.
The transcription map of …