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Full-Text Articles in Biochemistry
Purification And Characterization Of The Prostate Cancer-Associated Antigen: 7e11-C5, Qi Feng
Purification And Characterization Of The Prostate Cancer-Associated Antigen: 7e11-C5, Qi Feng
Theses and Dissertations in Biomedical Sciences
A murine monoclonal antibody (MAb), 7E11-C5, recognizes a prostate organ-specific antigen. Under reducing conditions, the 7E11-C5 antigen exhibited major 100-Kd and minor 70-Kd bands, suggesting that the antigen comprises two peptide chains. The antigen was detected in pooled normal human seminal plasma, xenograft tissue extract of a prostate adenocarcinoma cell line, LNCaP, and tissue extracts of benign prostatic hyperplasia (BPH) and prostate carcinomas, but was not identified in any of the non-prostate tissue extracts tested. Antibodies of the three major prostate-associated antigens (prostatic acid phosphatase (PAP), prostate specific antigen (PSA) and prostate secretory protein (PSP)) were unable to block the …