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Journal

2013

Articles 1 - 7 of 7

Full-Text Articles in Biochemistry

The Effects Of Salinity, Ph, Temperature, And Dissolved Oxygen On Sensitivity Of Pcr Identification Of T4 Bacteriophage, Joesph F. Cannon, Nicholas A. Thurn, Paul E. Richardson Dec 2013

The Effects Of Salinity, Ph, Temperature, And Dissolved Oxygen On Sensitivity Of Pcr Identification Of T4 Bacteriophage, Joesph F. Cannon, Nicholas A. Thurn, Paul E. Richardson

Journal of the South Carolina Academy of Science

Bacteriophages are used as indicators of pathogenic bacteria in drinking, and wastewaters. They also show potential in limiting aquatic bacterial populations through their lytic properties. The effect of different water characteristics (salinity, pH, dissolved oxygen, and temperature) on the sensitivity of the PCR identification of virus particles were analyzed to determine at what levels bacteriophage can be detected in environmental samples. Results from this preliminary study indicate that a PCR bacteriophage detection technique has potential as a relatively efficient and economical indicator of coliform contamination in multiple aquatic environments. While further evaluation is needed, the protocol appears to function in …

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Biochemistry / Histone Protein Modification - Purdue University, Jake R. Carlson, Katherine Beavis Aug 2013

Biochemistry / Histone Protein Modification - Purdue University, Jake R. Carlson, Katherine Beavis

Data Curation Profiles Directory

The researcher is conducting research on the brain cells of drosophila larvae. Specifically, the goal is to sort nuclei from glial and neuronal cells from the brains of mutant and wild type drosophila larvae. She will do transcriptome and high throughput ChIP-seq analysis on the cell nuclei to examine the distribution of complexes that modify histones and how loss of those complexes affects transcription. The stages of her research involve a long period of methodology development, a shorter data collection period, and then data analysis. The lab notebook contains written documentation of all experiments and trials and links to data, …

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Malnutrition In Sickle Cell Anemia: Implications For Infection, Growth And Maturation, Hyacinth I. Hyacinth, Oluwatoyosi A. Adekeye, Christopher S. Yilgwan Jan 2013

Malnutrition In Sickle Cell Anemia: Implications For Infection, Growth And Maturation, Hyacinth I. Hyacinth, Oluwatoyosi A. Adekeye, Christopher S. Yilgwan

Journal of Social, Behavioral, and Health Sciences

Sickle cell anemia (SCA) is a genetic disease that affects mostly individuals of African and/or Hispanic descent, with the majority of cases in sub-Saharan Africa. Individuals with this disease show slowed growth, delayed sexual maturity, and poor immunologic function. These complications could partly be explained by the state of undernutrition associated with the disease. Proposed mechanism of undernutrition include protein hypermetabolism, decreased dietary intake possibly from interleukin-6-related appetite suppression, increased cardiac energy demand/expenditure, and increased red cell turnover. All the above mechanisms manifest as increased resting energy expenditure. Nutritional intervention utilizing single or multiple nutrient supplementation has led to improved …

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The Cave Environment Influencing The Lipid Profile And Hepatic Lipogenesis Of The Fish Ancistrus Cryptophthalmus Reis, 1987 (Siluriformes: Loricariidae), Valquíria Aparecida Alves Bastos, Rodrigo Lopes Ferreira, Daniel Cardoso Carvalho, Marina Lages Pugedo, Luciana De Matos Alves Pinto Jan 2013

The Cave Environment Influencing The Lipid Profile And Hepatic Lipogenesis Of The Fish Ancistrus Cryptophthalmus Reis, 1987 (Siluriformes: Loricariidae), Valquíria Aparecida Alves Bastos, Rodrigo Lopes Ferreira, Daniel Cardoso Carvalho, Marina Lages Pugedo, Luciana De Matos Alves Pinto

International Journal of Speleology

The metabolism of hypogean organisms is frequently molded by the cave environment traits, especially food scarcity. The aim of the present work was to evaluate the influence of such environment on lipid composition and hepatic lipogenesis in the fish Ancistrus cryptophthalmus. For this, the species was compared to an epigean population of the same species. A greater accumulation of total lipids was observed in the cave-dwelling fish (18.36 g/100 g tissue) compared to the surface fish (14.09 g/100 g tissue). The muscle fatty acid profile also varied between the populations. Arachidonic acid was only detected in the epigean fish, while …

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Synthesis, Characterization, And Properties Of Mononuclear And Dinuclear Ruthenium(Ii) Complexes Containing Phenanthroline And Chlorophenanthroline, Anwar A. Bhuiyan, X. Du Jan 2013

Synthesis, Characterization, And Properties Of Mononuclear And Dinuclear Ruthenium(Ii) Complexes Containing Phenanthroline And Chlorophenanthroline, Anwar A. Bhuiyan, X. Du

Journal of the Arkansas Academy of Science

The study of photophysical and photochemical properties of ruthenium complexes is of great interest for fundamental practical reasons. Ruthenium complexes have been investigated for use in artificial photosynthesis. This paper deals with the synthesis and spectroscopic investigation of custom-designed ruthenium complexes containing phenanthroline and chloro-phenanthroline ligands. These complexes maybe useful for biological electron-transfer studies. The heteroleptic ruthenium monomer complex Ru(phen)2(Cl-phen) (where phen = 1,10-phenanthroline and Cl-phen=5-chloro-1,10-phenanthroline) was prepared in a two-step procedure previously developed in our laboratory. This monomer complex was used to prepare the ruthenium homometallic dimer complex, (phen)2Ru(phen-phen)Ru(phen)2, by utilizing the Ni-catalyzed coupling reaction. Both complexes were purified …

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Valence-Length Correlations For Chemical Bonds From Atomic Orbital Exponents, F. D. Hardcastle Jan 2013

Valence-Length Correlations For Chemical Bonds From Atomic Orbital Exponents, F. D. Hardcastle

Journal of the Arkansas Academy of Science

Pauling’s empirical bond valence-length correlation has proven valuable because it offers a quick and convenient way of checking and evaluating molecular structures and determining oxidation states from measured bond lengths. In this study, a simplified quantum-mechanical approach was used to derive Pauling’s empirical bond valence-length relationship by considering overlap of hydrogen-like orbitals. An expression for the b “empirical” fitting parameter was derived in terms of atomic-orbital exponents. A new set of orbital exponents is presented using published atomic/covalent radii and a continuous function for the effective principal quantum. The b parameters calculated from the orbital exponents are consistent with bond …

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Defending Your Dna: Combating Threats Both Foreign And Domestic, James Mcisaac Jan 2013

Defending Your Dna: Combating Threats Both Foreign And Domestic, James Mcisaac

Undergraduate Review

We are under constant assault from forces capable of damaging our DNA. The genetic code of DNA is made up of four nucleotides: adenine (A) which bonds with thymine (T) and guanine (G) which bonds with cytosine (C). If something happens to upset this normal pairing or the nucleotides themselves, our body must spring to action and respond to the damage. When damage to nucleotides prevents the normal replication machinery from doing its job, enzymes like the Y-family polymerases are called in. A special mechanism allows them to identify damage and insert the correct nucleotide or bypass the lesion to …

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