Open Access. Powered by Scholars. Published by Universities.®
- Discipline
-
- Chemistry (7)
- Physical Sciences and Mathematics (7)
- Physical Chemistry (4)
- Analytical Chemistry (1)
- Biogeochemistry (1)
-
- Biology (1)
- Chemicals and Drugs (1)
- Diseases (1)
- Earth Sciences (1)
- Environmental Chemistry (1)
- Environmental Health and Protection (1)
- Environmental Indicators and Impact Assessment (1)
- Environmental Sciences (1)
- Fresh Water Studies (1)
- Materials Chemistry (1)
- Medicinal Chemistry and Pharmaceutics (1)
- Medicine and Health Sciences (1)
- Natural Resources and Conservation (1)
- Neoplasms (1)
- Oceanography and Atmospheric Sciences and Meteorology (1)
- Organic Chemistry (1)
- Pharmacology, Toxicology and Environmental Health (1)
- Sedimentology (1)
- Sustainability (1)
- Water Resource Management (1)
- Institution
- Keyword
-
- Apolipoproteins (2)
- Biochemistry (2)
- DNA (2)
- Molecular dynamics (2)
- Protein folding (2)
-
- Algal bloom (1)
- Aluminum dosing (1)
- Amino Acids Fluorometric Scans (1)
- Biogeochemistry (1)
- CD spectroscopy (1)
- Cancer (1)
- Chemistry (1)
- Crowding Agents (1)
- Curcumin (1)
- Cyanine (1)
- Dendrimers; Ethylenediamine; Methyl acrylate (1)
- Dye (1)
- Emissive (1)
- Fanconi Anemia (1)
- Fluorescence (1)
- HPLC (1)
- High performance liquid chromatography; Amino acids (1)
- I-Motif (1)
- I-Motifs (1)
- Imaging (1)
- Indolizine (1)
- Kinetic (1)
- Laromustine (1)
- Oncology (1)
- Organic (1)
Articles 1 - 12 of 12
Full-Text Articles in Biochemistry
Analysis Of Caveolin-1 Expression And Interaction With Fshr In Lipid Raft Domains, Joseph Bott
Analysis Of Caveolin-1 Expression And Interaction With Fshr In Lipid Raft Domains, Joseph Bott
Honors Theses
For thousands of women in the United States ovarian cysts, tumors, and hormonal imbalances contribute to reduced fertility and fecundity. In addition to these factors, many other unexplained physiological mechanisms may play a role in women’s reproductive health. Specifically, human follicle stimulating hormone (hFSH) binds to a G protein coupled receptor (GPCR) called human follicle stimulating hormone receptor (hFSHR). This interaction helps to regulate the reproductive system in both males and females indicating that it may be a key player to target in order to promote fertility. Previous work in our lab has demonstrated that hFSHR, like other GPCR, has …
Effects Of Crowding Agents On I-Motif Dna, Hayden Brines
Effects Of Crowding Agents On I-Motif Dna, Hayden Brines
Honors Theses
Deoxyribonucleic acid (DNA) is a well-known double stranded, helical, biological molecule. In addition to its more commonly known structure, DNA can also form more complicated structures like G-quadruplexes and i-motifs (iM). The iMs are formed by cytosine rich DNA and are a four stranded structure that is typically looped around itself. The iM formation is typically pH-dependent and is favored in more acidic conditions; the pKa value is approximately 6.5. This pKa value allows for potential in vivo formation, since the cells have a pH of approximately 7.3. Due to this, iMs are thought to be powerful, innovative molecules for …
Covalent Modification Of Genetically Engineered Polymers With Bodipy Fluorophores, Emilie Smith
Covalent Modification Of Genetically Engineered Polymers With Bodipy Fluorophores, Emilie Smith
Honors Theses
Osteoarthritis and other debilitating diseases cause chronic pain that is difficult to manage. Nonsteroidal Anti-inflammatory Drugs (NSAIDs) are the current standard of treatment for many long-term pain management, but there are potential systemic effects when using NSAIDs long-term. Therefore, it isnecessary to have an alternative option for NSAID delivery that doesn’t include these systemic effects. Hydrogels can be that alternative option. Hydrogels’ distinct characteristics allow them to be viable in the body, degrade under unique conditions, and be modified through many different chemistries. Additionally, hydrogels can be engineered and their properties optimized for the use as a drug depot inside …
Chemical And Co-Solute Effects Of Polyethylene Glycol On I-Motif Formation, Lindsey Rutherford
Chemical And Co-Solute Effects Of Polyethylene Glycol On I-Motif Formation, Lindsey Rutherford
Honors Theses
DNA typically forms Watson and Crick double helix structures in which adenine, thymine, guanine, and cytosine pair with their complimentary DNA base. However, DNA i-motif structures can form in cytosine rich DNA, typically under slightly acidic conditions (~pH 6). DNA i-motifs are four stranded secondary structures in which cytosine pairs with cytosine to form a quadruplex. The i-motifs are typically formed in acidic conditions because of the protonation in the C•C base pair between one of the three hydrogen bases. Recent studies have suggested i-motifs can also form under neutral conditions, which is more realistic for a cell. It is …
The Effect Of The Apolipoprotein A1 (Apoa1): The Stability And Folding In Potassium Chloride Environment, Alexandra Paladian
The Effect Of The Apolipoprotein A1 (Apoa1): The Stability And Folding In Potassium Chloride Environment, Alexandra Paladian
Honors Theses
Healthy levels of potassium chloride (KCl) can significantly affect the workings of the cholesterol level of the human body and how they pertain to an individual person. The search for a better salt additive for the human diet can provide a better option for people who experience high cholesterol levels and heart disease. The study focuses on the experimental design of the Molecular Dynamic (MD) simulation of the Apolipoprotein A1 (APOA1) in the potassium ion solution environment to determine the stability and folding of the protein. The study also compares its data to the previous experimental design of chloride ions …
Molecular Dynamic Simulation Of The Complex Folding Patterns Of Apolipoprotein A1 In Various Concentrations Of Potassium Chloride, Hannah Holmberg
Molecular Dynamic Simulation Of The Complex Folding Patterns Of Apolipoprotein A1 In Various Concentrations Of Potassium Chloride, Hannah Holmberg
Honors Theses
Apopliprotein or ApoA-1 is a complex lipoprotein that functions in the removal of cholesterol from the blood, removing cholesterol from the area around white blood cells and promoting the excretion of lipids through the lymphatic system. Previous research has found that ApoA-1 shows both folded and unfolded conformations depending on the concentration of NaCl in solution in the water around it. The protein was studied using molecular dynamics simulations. Once this state of equilibrium was reached, various structural properties of the protein were measured including the radius of gyration and the radial distribution function. The goal of the project was …
Finding Optimal Opa-Iblc Derivatized Amino Acid Analysis Parameters With Three-Dimensional Fluorometric Scans For Hplc Method Validation, Aaron Jacobs
Honors Theses
Three-dimensional fluorometric scans of OPA-IBLC derivatized glutamic acid and histidine solutions revealed that decreasing concentration maintained maximum λem but produced different maximum λex. In pH 0.4 M sodium borate buffer (pH 10.4), 50 μM derivatized amino acid solutions had maxima around 350- to 450-nm λex-λem where 12.5 μM solutions had maxima around 230- to 450-nm λex- λem. In the lower concentration, the λex peak at 230 nm was about 80% higher than 350 nm. Thus, collecting spectra with 230 nm λex would provide better LOD and LOQ. Results inform HPLC standard concentrations since differential quenching effects may produce non-linear calibration …
Thermodynamic Analysis Of Pamam Dendrimer Synthesis, Ryutaro Einar Jacobson
Thermodynamic Analysis Of Pamam Dendrimer Synthesis, Ryutaro Einar Jacobson
Honors Theses
The objective of this research was to determine kinetic and thermodynamic data about the reaction between ethylene diamine and methyl acrylate which is used in the production of PAMAM dendrimers. Establishing a rate law and finding values such as rate constant, activation energy, and Arrhenius coefficient will help in determining the best possible conditions for the reaction. A rate law was established through the method of initial rates, comparing the rates of reactions with different initial concentrations to one another. Additional reactions were run with active cooling to observe the effects on rate and reaction progress, and finally reactions were …
Construction Of E. Coli Strain With N-Terminal Domain Deletion In The Biotin Carboxyl Carrier Protein, Dara Khosravi
Construction Of E. Coli Strain With N-Terminal Domain Deletion In The Biotin Carboxyl Carrier Protein, Dara Khosravi
Honors Theses
No abstract provided.
Indolizine Donor-Based Dyes For Applications In Fluorescence Biological Imaging, William Meador
Indolizine Donor-Based Dyes For Applications In Fluorescence Biological Imaging, William Meador
Honors Theses
NIR emissive fluorophores are intensely researched due to their potential to replace modern imaging procedures. Many molecular strategies have been employed in the literature to optimize fluorophores for deeper NIR absorption and emission, biocompatibility, and higher fluorescence quantum yields. Amongst the fluorophores studied to date, proaromatic indolizine donors are attractive alternatives to traditional alkyl amine and indoline based donors due to their 1) lower energy absorption and emission facilitated by proaromaticity, 2) large Stokes shifts due to increased dihedral angles about the π-system, 3) ease of functionalization and capacity for bioconjugation at the phenyl ring, and 4) potential for further …
Use Of Small Molecule Fanconi Anemia Pathway Inhibitors As Sensitizing Agents To Laromustine., Sam W. Marchant
Use Of Small Molecule Fanconi Anemia Pathway Inhibitors As Sensitizing Agents To Laromustine., Sam W. Marchant
Honors Theses
Laromustine is an experimental chemotherapeutic sulfonyl hydrazine prodrug shown in clinical trials to be effective against acute myeloid leukemia. The mechanism of action of laromustine involves interstrand crosslinking, via chloroethylation, and enzyme inhibition, caused by carbamoylation. The work described herein aims to investigate whether inhibition of the replication-dependent interstrand crosslink repair Fanconi Anemia pathway further sensitizes cells to laromustine. By measuring metabolic activity immediately after drug exposure, we find laromustine to be equally as cytotoxic towards Fanconi Anemia deficient and wild type cells. However, through clonogenic assays we show Fanconi Anemia mutations sensitize cells to laromustine’s anti-proliferative effect. Furthermore, we …
The Quantitative Assessment Of Pond Scum: An Examination Of The Biogeochemistry Of Phosphorus Cycling In The Belgrade Lakes, Abbey M. Sykes
The Quantitative Assessment Of Pond Scum: An Examination Of The Biogeochemistry Of Phosphorus Cycling In The Belgrade Lakes, Abbey M. Sykes
Honors Theses
The internal recycling phosphorus in freshwater lake bottom sediments represents a significant source of hypolimnetic phosphorus (P) release for many of Maine’s lakes. In summer months, Maine lakes often thermally stratify and the lake hypolimnion develops anoxia, leading to a reduction in redox potential at the sediment-water interface. These reducing conditions facilitate the reductive dissolution of ferric iron, and, since phosphorus is often present in freshwater lake sediments as solid FeOOH-PO4 complexes, results in release of soluble phosphorus into the water column. Our current study presents field and laboratory data from sediment fractionation extractions designed to quantify concentrations of …