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Full-Text Articles in Biochemistry
Investigation Of Catalytic Activity Of Stable Β-Ketoacyl-Acp Substrate Analogs In Quorum Sensing Signal Synthesis, Nhu Lam
Boise State University Theses and Dissertations
Quorum sensing is an intercellular mechanism used by many bacterial pathogens to regulate behaviors, such as motility, virulence, and antibiotic production. Disruption of quorum sensing is shown to be deleterious to bacterial pathogenicity, without causing lethality. In Gram-negative bacteria, acyl-homoserine lactone (AHL or acyl-HSL) signal molecules are synthesized by AHL synthase enzymes using acylated-acyl carrier protein (acyl-ACP) and S-adenosyl-L-methionine (SAM) as their substrates. Pantoea stewartii EsaI, which causes Stewart’s wilt in corn, and Yersinia pestis YspI, which causes bubonic plague are AHL synthases that use 3-oxohexanoyl-ACP and 3-oxooctanoyl-ACP as the acyl-substrate to synthesize 3-oxohexanoyl-HSL and 3-oxooctanoyl-HSL signals, respectively. Unfortunately, the …
Mechanistic And Substrate Specificity Studies On Burkholderia Mallei Quorum Sensing Signal Synthesis Enzyme, Aubrey N. Montebello
Mechanistic And Substrate Specificity Studies On Burkholderia Mallei Quorum Sensing Signal Synthesis Enzyme, Aubrey N. Montebello
Boise State University Theses and Dissertations
Gram-negative bacteria use acyl-homoserine lactone (AHL) based quorum sensing (QS) to regulate the expression of genes that give the bacteria a selective advantage over host defenses and antibiotic treatment. Burkholderia mallei is an antibiotic resistant pathogen that causes Glanders disease. B. mallei BmaI1 AHL-synthase uses octanoyl-Acyl Carrier Protein (C8ACP) and S-adenosyl-L-methionine (SAM) to synthesize the AHL, octanoyl-homoserine lactone (C8HSL). Inhibiting AHL-synthases has been difficult because mechanistic and substrate specificity details for these enzymes are not well understood. Our goal was to determine how BmaI1 activity and enzymatic mechanism changes with nonspecific, variable acyl chain acyl-ACP substrates. We found that catalytic …