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Mechanistic And Substrate Specificity Studies On Burkholderia Mallei Quorum Sensing Signal Synthesis Enzyme, Aubrey N. Montebello
Mechanistic And Substrate Specificity Studies On Burkholderia Mallei Quorum Sensing Signal Synthesis Enzyme, Aubrey N. Montebello
Boise State University Theses and Dissertations
Gram-negative bacteria use acyl-homoserine lactone (AHL) based quorum sensing (QS) to regulate the expression of genes that give the bacteria a selective advantage over host defenses and antibiotic treatment. Burkholderia mallei is an antibiotic resistant pathogen that causes Glanders disease. B. mallei BmaI1 AHL-synthase uses octanoyl-Acyl Carrier Protein (C8ACP) and S-adenosyl-L-methionine (SAM) to synthesize the AHL, octanoyl-homoserine lactone (C8HSL). Inhibiting AHL-synthases has been difficult because mechanistic and substrate specificity details for these enzymes are not well understood. Our goal was to determine how BmaI1 activity and enzymatic mechanism changes with nonspecific, variable acyl chain acyl-ACP substrates. We found that catalytic …