Biochemistry Commons^™

Crystal Structures Of The Streptomyces Coelicolor Tetr-Like Protein Actr Alone And In Complex With Actinorhodin Or The Actinorhodin Biosynthetic Precursor (S)-Dnpa., A R Willems, K Tahlan, T Taguchi, K Zhang, Z Z Lee, K Ichinose, M S Junop, J R Nodwell

Biochemistry Publications

Actinorhodin, an antibiotic produced by Streptomyces coelicolor, is exported from the cell by the ActA efflux pump. actA is divergently transcribed from actR, which encodes a TetR-like transcriptional repressor. We showed previously that ActR represses transcription by binding to an operator from the actA/actR intergenic region. Importantly, actinorhodin itself or various actinorhodin biosynthetic intermediates can cause ActR to dissociate from its operator, leading to derepression. This suggests that ActR may mediate timely self-resistance to an endogenously produced antibiotic by responding to one of its biosynthetic precursors. Here, we report the structural basis for this precursor-mediated derepression with crystal structures of …