Open Access. Powered by Scholars. Published by Universities.®
- Discipline
- Keyword
-
- Acetaminophen (1)
- Amyloid fibril (1)
- Beta-sheet folding (1)
- Biochemical toxicology (1)
- CYP2A5 (1)
-
- Chaperone activity (1)
- Cytochrome P-450 (1)
- De novo protein design (1)
- Double Electron-Electron Resonance (DEER) (1)
- Drugs (1)
- Electron paramagnetic resonance (EPR) (1)
- Enzymatic analysis (1)
- Enzymatic probing (1)
- Footprinting (1)
- Genetic engineering (1)
- Genetical engineering (1)
- HIV (Viruses) (1)
- HIV antibodies (1)
- Homeostasis (1)
- Human immunodeficiency virus type 1(HIV 1) (1)
- Knock-out (1)
- Mice (1)
- Model polypeptides (1)
- NCp7 (1)
- Nicotine (1)
- Nucleocapsid protein (NC) (1)
- Polypeptides (1)
- Propionic acid (1)
- Protein aggregation (1)
- Purification of rna (1)
Articles 1 - 4 of 4
Full-Text Articles in Biochemistry
Genetically Engineered Repetitive Polypeptides : Design, Synthesis, Characterization, And Applications, Natalya I. Topilina
Genetically Engineered Repetitive Polypeptides : Design, Synthesis, Characterization, And Applications, Natalya I. Topilina
Legacy Theses & Dissertations (2009 - 2024)
This work is dedicated to the investigation of protein folding with the emphasis on the aggregation and amyloid fibrils formation. The focus of the research is to elucidate the relationship between polypeptide chain sequence and folding/aggregation properties. For this purpose, i) libraries of the de novo macromolecular repetitive β-sheet forming polypeptides were designed, and ii) the strategy for their rapid creation was developed. The strategy enables retention of a basic polypeptide core, yet allowing variations in the repetitive sequence, degree of polymerization and distribution of specific amino acids. The polypeptides were expressed in E. coli, purified, and characterized by various …
Role Of Cyp2a5 In Drug Metabolism, Chemical Toxicity, And Maintenance Of Steroid Hormone Homeostasis : Insights From Studies On A Novel Cyp2a5-Null Mouse Model, Xin Zhou
Legacy Theses & Dissertations (2009 - 2024)
The central hypothesis is that CYP2A5 plays an important role in the metabolism of xenobiotic substrates, and in the toxicity induced by over-exposure to drugs, as well as in the metabolism of endogenous compounds and regulation of steroid hormone homeostasis. The specific aims are: 1) to generate and characterize a Cyp2a5-null mouse; 2) to determine the role of CYP2A5 in the systemic clearance of nicotine and cotinine; and 3) to explore the mechanisms underlying the resistance of the lateral nasal gland (LNG) of male Cyp2g1-null/Cyp2a5-low mouse and Cyp2a5-null mouse to acetaminophen (AP) toxicity.
Secondary Structures And Thermodynamic Properties Of Ampa Receptor Aptamers, Sabarinath Jayaseelan
Secondary Structures And Thermodynamic Properties Of Ampa Receptor Aptamers, Sabarinath Jayaseelan
Legacy Theses & Dissertations (2009 - 2024)
Using systematic evolution of ligands by exponential enrichment (SELEX), our lab previously selected a class of competitive RNA aptamers against the alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptors. One aptamer in this class was found to have a minimal, functional sequence of 58 nucleotides. In vitro transcription generates two RNA transcripts with the same sequence, which we named M1 and M2, both of which are required to work together to inhibit the AMPA receptors. Although the M1 and M2 species have the same sequence, they cannot be interconverted through unfolding by denaturation/refolding by renaturation. To probe the secondary structures and the thermodynamic …
The Recognition And Folding Of Ncp7 (Nucleocapsid Protein) With Tardna And Psi Site Rna Hairpin Structures From Hiv-1 : A Spin Label Study, Yan Sun
Legacy Theses & Dissertations (2009 - 2024)
The nucleocapsid (NC) protein NCp7 of the human immunodeficiency virus type 1(HIV 1) is a small basic protein with two zinc finger motifs. NCp7 has key roles in virus replication and structure, which rely on its interactions with nucleic acids. We investigated the interaction of NCp7 with Transactivation Response Element (TAR) DNA which is critical for minus strand transfer during the HIV 1 reverse transcription. The EPR spin probe nitroxide was used as the reporter. Binding of NCp7 to TARDNA caused TARDNA condensation, as inferred from the probe tumbling time which markedly increased to several nanoseconds at a 4:1 NCp7 …