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Rna Interference Of Ctla4 In Human Leukaemia T Cells, Ralitsa Vassileva
Rna Interference Of Ctla4 In Human Leukaemia T Cells, Ralitsa Vassileva
Doctoral
The activation of naive T cells is essential for the initiation of the adaptive immunity. One promising approach to overcome immunological tolerance involves augmenting endogenous T cell-mediated immunity by interrupting the T cell down-regulatory pathways. In this project a gene therapy delivery system to overexpress small interfering RNA was used to silence the inhibitory signals induced by CTLA4. A small hairpin RNA duplex designed to silence expression of CTLA4 was successfully cloned into a plasmid. A stably transfected T cell line that is expressing constitutively the CTLA4 siRNA has been developed as a model to understand the role of CTLA4 …