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Full-Text Articles in Biochemistry
Carbon Monoxide And Nitric Oxide Induced-Heme-Based Modification Of Alpha-2-Antiplasmin And Plasmin Activity, Matthew R. Arkebauer
Carbon Monoxide And Nitric Oxide Induced-Heme-Based Modification Of Alpha-2-Antiplasmin And Plasmin Activity, Matthew R. Arkebauer
PCOM Biomedical Studies Student Scholarship
Objectives: Carbon monoxide (CO) derived from cigarette smoke or released from carbon monoxide releasing-molecule 2 (CORM-2), diminishes fibrinolysis. The primary purpose of this study was to determine if CO diminished fibrinolysis by means of enhancing α2-antiplasmin via an alleged heme group. Methods: Plasma, isolated α2-antiplasmin and isolated plasmin were exposed to CO released from CORM-2 (tricarbonyldichlororuthenium (II) dimer) and nitric oxide (NO) via a NO donor to induce carboxyheme and metheme states. Exposed, isolated enzymes were placed in either α2-antiplasmin deficient or normal plasma. Effects of CO and NO on tissue-type plasminogen activator initiated fibrinolysis were determined by thrombelastography. Liquid …