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Articles 1 - 5 of 5
Full-Text Articles in Biochemistry
Studies Of N⁵, N¹⁰-Methylene Tetrahydrofolate Reductase From Porcine Kidney And Mouse L1210-Induced Tumor Tissues, Purification And Interaction With Antifolates, David W. Jayme
Theses and Dissertations
Methylene tetrahydrofolate reductase has been purified 1000-fold from porcine kidney and 400-fold from mouse L1210-induced tumor tissue by classical methods. The enzyme preparations have been demonstrated to be essentially free of contaminating methionine synthetase and serine transhydroxymethylase activity. Studies of the kinetic properties of the kidney and tumor enzymes, with respect to the reverse reaction using N5-methyl tetrahydrofolate as the variable substrate, have indicated Km values of 2.0 and 2.4 x 10-4 M, respectively. Inhibition of this key branch point enzyme in folate metabolism by a number of antimetabolites indicates that several of these antifolate compounds exhibit enzyme inhibition superior …
Fractionation Of Ribosomal Proteins From Mesophilic And Thermophilic Bacteria, Raymond J. Knaus
Fractionation Of Ribosomal Proteins From Mesophilic And Thermophilic Bacteria, Raymond J. Knaus
Masters Theses
No abstract provided.
The Role Of Zinc In Dihydroorotase, Pamela S. Gilchrist
The Role Of Zinc In Dihydroorotase, Pamela S. Gilchrist
Dissertations and Theses
Dihydroorotase (4,4—dihydroorotic acid amidolyase, EC 3.5.2.3.) which catalyzes the reversible cyclization of N-carbamyl-L-aspartate to L-dihydroorotate has been purified from orotate-grown Clostridium oroticum. The enzyme is stable in 0.3 M sodium chloride and 10 µ ZnSO4. Sodium dodecyl sulfate gel electrophoresis indicates the enzyme to be composed of two identical subunits each with a molecular weight of 58,000 + 6000. Dihydroorotase is shown to be a zinc-containing metalloenzyme with 2 g atoms of zinc per 58,000 g of protein. The role of zinc in dihydroorotase is discussed.
The Isolation And Partial Structure Identification Of A Polar Glycosphingolipid Of Rabbit Blood, Michael B. Shubeck
The Isolation And Partial Structure Identification Of A Polar Glycosphingolipid Of Rabbit Blood, Michael B. Shubeck
Masters Theses
No abstract provided.
The Presence Of Acid Polysaccharides In The Mesogloea Of Chrysaora Quinquecirrha, Edward P. Gardner
The Presence Of Acid Polysaccharides In The Mesogloea Of Chrysaora Quinquecirrha, Edward P. Gardner
Dissertations, Theses, and Masters Projects
No abstract provided.