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Full-Text Articles in Biochemistry
The Effects Of Framework Mutations At The Variable Domain Interface On Antibody Affinity Maturation In An Hiv-1 Broadly Neutralizing Antibody Lineage, Jeffrey O. Zhou , '19, Hussain A. Zaidi , '22, Therese Ton , '19, Daniela Fera
The Effects Of Framework Mutations At The Variable Domain Interface On Antibody Affinity Maturation In An Hiv-1 Broadly Neutralizing Antibody Lineage, Jeffrey O. Zhou , '19, Hussain A. Zaidi , '22, Therese Ton , '19, Daniela Fera
Chemistry & Biochemistry Faculty Works
Understanding affinity maturation of antibodies that can target many variants of HIV-1 is important for vaccine development. While the antigen-binding site of antibodies is known to mutate throughout the co-evolution of antibodies and viruses in infected individuals, the roles of the mutations in the antibody framework region are not well understood. Throughout affinity maturation, the CH103 broadly neutralizing antibody lineage, from an individual designated CH505, altered the orientation of one of its antibody variable domains. The change in orientation was a response to insertions in the variable loop 5 (V5) of the HIV envelope. In this study, we generated CH103 …
Identification Of Novel Autoinducer-2 Receptors In Clostridia Reveals Plasticity In The Binding Site Of The Lsrb Receptor Family, I. M. Torcato, M. R. Kasal, P. H. Brito, Stephen T. Miller, K. B. Xavier
Identification Of Novel Autoinducer-2 Receptors In Clostridia Reveals Plasticity In The Binding Site Of The Lsrb Receptor Family, I. M. Torcato, M. R. Kasal, P. H. Brito, Stephen T. Miller, K. B. Xavier
Chemistry & Biochemistry Faculty Works
Autoinducer-2 (AI-2) is unique among quorum-sensing signaling molecules, as it is produced and recognized by a wide variety of bacteria and thus facilitates interspecies communication. To date, two classes of AI-2 receptors have been identified: the LuxP-type, present in the Vibrionales, and the LsrB-type, found in a number of phylogenetically distinct bacterial families. Recently, AI-2 was shown to affect the colonization levels of a variety of bacteria in the microbiome of the mouse gut, including members of the genus Clostridium, but no AI-2 receptor had been identified in this genus. Here, we identify a noncanonical, functional LsrB-type receptor in Clostridium …