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Biochemistry Commons

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Full-Text Articles in Biochemistry

Functional Characterization Of The Arabidopsis Β-Ketoacyl-Coenzyme A Reductase Candidates Of The Fatty Acid Elongase, Frédéric Beaudoin, Xianzhong Wu, Fengling Li, Richard P. Haslam, Jennifer E. Markham, Huanquan Zheng, Johnathan A. Napier, Ljerka Kunst Jul 2009

Functional Characterization Of The Arabidopsis Β-Ketoacyl-Coenzyme A Reductase Candidates Of The Fatty Acid Elongase, Frédéric Beaudoin, Xianzhong Wu, Fengling Li, Richard P. Haslam, Jennifer E. Markham, Huanquan Zheng, Johnathan A. Napier, Ljerka Kunst

Department of Biochemistry: Faculty Publications

In plants, very-long-chain fatty acids (VLCFAs; >18 carbon) are precursors of sphingolipids, triacylglycerols, cuticular waxes, and suberin. VLCFAs are synthesized by a multiprotein membrane-bound fatty acid elongation system that catalyzes four successive enzymatic reactions: condensation, reduction, dehydration, and a second reduction. A bioinformatics survey of the Arabidopsis (Arabidopsis thaliana) genome has revealed two sequences homologous to YBR159w encoding a Saccharomyces cerevisiae β-ketoacyl reductase (KCR), which catalyzes the first reduction during VLCFA elongation. Expression analyses showed that both AtKCR1 and AtKCR2 genes were transcribed in siliques, flowers, inflorescence stems, leaves, as well as developing embryos, but only AtKCR1 …


Functional Analysis Of Receptor-Like Kinases In Pollen-Pistil Interactions In Arabidopsis Thaliana, Mini Aggarwal Jan 2009

Functional Analysis Of Receptor-Like Kinases In Pollen-Pistil Interactions In Arabidopsis Thaliana, Mini Aggarwal

Masters Theses 1911 - February 2014

Rac/Rop GTPases are molecular switches in plants that control the growth of polarized cells such as pollen tubes and root hairs, differentiation, development, actin dynamics, production of reactive oxygen species (ROS) and disease resistance. These small GTPases are activated by guanine nucleotide exchange factors (GEFs) that replace GDP for GTP and are referred to as RopGEFs in plants. To identify upstream components of the RopGEF regulated signaling pathways, GEF1 from Arabidopsis thaliana was used as a bait to screen a seedling cDNA library in a yeast two-hybrid system. This yielded members of a small family of the Catharanthus roseus Receptor-like …


Letter From The Dean, Lalit Verma Jan 2009

Letter From The Dean, Lalit Verma

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.