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Full-Text Articles in Biochemistry
Determining The Full-Length Structure Of Collagenase H Using Small-Angle X-Ray Scattering, Josie Carson
Determining The Full-Length Structure Of Collagenase H Using Small-Angle X-Ray Scattering, Josie Carson
Chemistry & Biochemistry Undergraduate Honors Theses
Known to cause gas gangrene, Hathewaya histolytica secretes two sister collagenases, collagenase G (Col G) and collagenase H (Col H), to degrade the triple helical structure of collagen to further infection in a host. Individual domains of Col H have been crystalized in previous studies, but methods in x-ray crystallization of full-length Col H have been unsuccessful. Using Small Angle X-Ray Scattering (SAXS) data, atomistic modeling was used to generate multiple conformations of Col H while accounting for flexibility between domains. Full-length Col H was found to adopt a two-state conformational model exhibiting a majority compact and a minority elongated …
Hyper Stable Variants Of Fgf-1-Fgf-2 Dimer, Madison Shields Mcclanahan
Hyper Stable Variants Of Fgf-1-Fgf-2 Dimer, Madison Shields Mcclanahan
Chemistry & Biochemistry Undergraduate Honors Theses
Fibroblast Growth Factors (FGFs), including FGF-1 and FGF-2, are proteins that play a crucial role in cell proliferation, cell differentiation, cell migration, and tissue repair. FGF-1 and FGF-2 are useful in accelerating the healing process in the human body; however, these proteins are naturally thermally unstable, resulting in a relatively low half-life in vivo. 1,8 In efforts to improve the stability of this protein, FGF-1 and FGF-2 proteins are engineered by combining the amino acid sequences of the two proteins to form a heterodimer and obtain novel properties. These two FGF variants are chosen for their specific wound healing capabilities. …