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- Adherens junction (1)
- Atomic structures (1)
- Beta strands (1)
- Cadherins (1)
- Cryo electron microscopy (1)
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- Electron microscopy maps (1)
- Fitting (1)
- Focal adhesions (1)
- Identification (1)
- Integrins (1)
- Magnetic cytometry (1)
- Mechanotransduction (1)
- Protein structure (1)
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- Resolution density maps (1)
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- Shear flow microfluidic device (1)
- Situs (1)
- Structural informatics approach (1)
- Structure elements (1)
- Traction force microscopy (1)
- Validation (1)
Articles 1 - 2 of 2
Full-Text Articles in Biochemistry
Biophysical Tools To Study Cellular Mechanotransduction, Ismeel Muhamed, Farhan Chowdhury, Venkat Maruthamuthu
Biophysical Tools To Study Cellular Mechanotransduction, Ismeel Muhamed, Farhan Chowdhury, Venkat Maruthamuthu
Mechanical & Aerospace Engineering Faculty Publications
The cell membrane is the interface that volumetrically isolates cellular components from the cell's environment. Proteins embedded within and on the membrane have varied biological functions: reception of external biochemical signals, as membrane channels, amplification and regulation of chemical signals through secondary messenger molecules, controlled exocytosis, endocytosis, phagocytosis, organized recruitment and sequestration of cytosolic complex proteins, cell division processes, organization of the cytoskeleton and more. The membrane's bioelectrical role is enabled by the physiologically controlled release and accumulation of electrochemical potential modulating molecules across the membrane through specialized ion channels (e.g., Na⁺, Ca2+, K⁺ channels). …
Numerical Geometry Of Map And Model Assessment, Willy Wriggers, Jing He
Numerical Geometry Of Map And Model Assessment, Willy Wriggers, Jing He
Mechanical & Aerospace Engineering Faculty Publications
We are describing best practices and assessment strategies for the atomic interpretation of cryo-electron microscopy (cryo-EM) maps. Multiscale numerical geometry strategies in the Situs package and in secondary structure detection software are currently evolving due to the recent increases in cryo-EM resolution. Criteria that aim to predict the accuracy of fitted atomic models at low (worse than 8 angstrom) and medium (4-8 angstrom) resolutions remain challenging. However, a high level of confidence in atomic models can be achieved by combining such criteria. The observed errors are due to map-model discrepancies and due to the effect of imperfect global docking strategies. …