Biochemistry Commons^™

Altering The Rna Binding Specificity Of A Translational Repressor, F Lim, Marc Spingola, D Peabody

Biology Department Faculty Works

The coat proteins of RNA phages MS2 and GA are specific RNA-binding proteins which function to encapsidate viral RNA and to translationally repress synthesis of the viral replicase. The two proteins have highly homologous amino acid sequences, yet they show different RNA binding specificities, recognizing RNA stem-loop structures which differ primarily in the nucleotide sequences of their loops. We sought to convert MS2 coat protein to the RNA binding specificity of GA through the introduction of GA-like amino acid substitutions into the MS2 coat protein RNA-binding site. The effects of the mutations were determined by measuring the affinity of the …

Altering The Rna Binding Specificity Of A Translational Repressor, F Lim, Marc Spingola, D S. Peabody

Marc Spingola

Study Of Existing Information Concerning Water Quality Within Lake Mead, Vicki Scharnhorst, Southern Nevada Water Authority

Publications (WR)

The purpose of Task 010A15M of the Southern Nevada Water Authority (SNWA) Treatment and Transmission Facility (TTF) contract is to conduct a study of existing information concerning water quality within Lake Mead and identify additional water quality studies that are needed to supplement existing data.

The objective of this task is not to discuss treatability of the raw water source; this is addressed by Task 010A18M, "Define Water Treatment Requirements." In addition, a narrative on the effect of pending Safe Drinking Water Act amendments and a determination of treated water quality goals is included in Task 010A16M, "Review Safe Drinking …