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2024

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Articles 691 - 696 of 696

Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Structural And Functional Analysis Of The Putatively Virulent Gene Products Tde1414, Tde1511, And Tde2714 From The Oral Pathogen Treponema Denticola, Brock W. Corbett Jan 2024

Structural And Functional Analysis Of The Putatively Virulent Gene Products Tde1414, Tde1511, And Tde2714 From The Oral Pathogen Treponema Denticola, Brock W. Corbett

Theses and Dissertations (Comprehensive)

Periodontitis is a destructive polymicrobial disease of the teeth and gums characterized by dental plaque biofilms on the tooth surface, severe inflammation of surrounding gum tissues, and the resorption of alveolar bone. Discussed as one of the most prevalent conditions plaguing humankind, increased clinical parameters of periodontitis is associated with the increased presence of three bacteria known as the “red complex”: Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola. Various transcriptomic and proteomic studies of T. denticola when incorporated into mature biofilms have illuminated key gene targets the bacterium may use as virulence factors to aid in pathogenesis. …

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Characterizing The Targeting Pathway Of Chloroplast Outer Membrane Protein Oep18 In Arabidopsis Thaliana And Other Targeting Signals Utilized By Outer Envelope Membrane Proteins, Ceaira Hiemstra Jan 2024

Characterizing The Targeting Pathway Of Chloroplast Outer Membrane Protein Oep18 In Arabidopsis Thaliana And Other Targeting Signals Utilized By Outer Envelope Membrane Proteins, Ceaira Hiemstra

Theses and Dissertations (Comprehensive)

Approximately 95% of chloroplast proteins are encoded by nuclear DNA and are post-translationally targeted to the chloroplast. Over half of the 138 known or predicted chloroplast outer membrane (OM) proteins are predicted to utilize four non-canonical targeting pathways. The first two pathways are characterized by containing a single α-helical transmembrane domain (TMD) within the first or last 50 amino acids (AAs), the third pathway is utilized by β-barrels, and the last requires an N-terminal cleavable transit peptide (TP). Recently, a fifth novel pathway called Toc159-like (TOC159L) has been characterized by the presence of a C-terminal reverse TP in Toc159. Subsequent …

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Inactive Metallopeptidase Homologs: The Secret Lives Of Pseudopeptidases, Peter Lyons Jan 2024

Inactive Metallopeptidase Homologs: The Secret Lives Of Pseudopeptidases, Peter Lyons

Faculty Publications

Inactive enzyme homologs, or pseudoenzymes, are proteins, found within most enzyme families, that are incapable of performing catalysis. Rather than catalysis, they are involved in protein-protein interactions, sometimes regulating the activity of their active enzyme cousins, or scaffolding protein complexes. Pseudoenzymes found within metallopeptidase families likewise perform these functions. Pseudoenzymes within the M14 carboxypeptidase family interact with collagens within the extracellular space, while pseudopeptidase members of the M12 "a disintegrin and metalloprotease" (ADAM) family either discard their pseudopeptidase domains as unnecessary for their roles in sperm maturation or utilize surface loops to enable assembly of key complexes at neuronal synapses. …

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Exploring The Molecular Pathways Of Cell Death Induced By Pde3 Modulators, Aqsa Ahsan Jan 2024

Exploring The Molecular Pathways Of Cell Death Induced By Pde3 Modulators, Aqsa Ahsan

Dissertations, Master's Theses and Master's Reports

Metabolic dysregulation, a critical hallmark of cancer, predisposes potential vulnerabilities for the development of targeted therapy. LKB1, a pivotal metabolic regulator and tumor suppressor, is often lost in many cancers. Our laboratory has unveiled that LKB1 suppresses phosphodiesterases (PDEs), and PDE3 modulators can selectively eradicate LKB1-deficient tumor cells. Through metabolic gene sgRNA library screening, Sarcolipin, an inhibitor of Sarcoendoplasmic Reticulum Calcium ATPase (SERCA), emerged as essential for cell death in this context. However, the precise molecular mechanisms by which PDE3 modulators exert their effects remain elusive. This study aims to uncover these mechanisms via RNA-Seq profiling and differential gene analysis, …

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A Multimolecular Perspective On The Success Of Wolf Recovery And Relocation, Samuel D. Hervey Jan 2024

A Multimolecular Perspective On The Success Of Wolf Recovery And Relocation, Samuel D. Hervey

Dissertations, Master's Theses and Master's Reports

With global biodiversity in decline and many species isolated in fragmented habitats, conservation efforts increasingly rely on natural recolonization and translocation to restore ecosystems. These strategies face challenges such as genetic erosion and habitat limitations, highlighting the need for effective monitoring practices. This research assesses the success of wolf recovery initiatives by examining long-term genetic and genomic health using both traditional and advanced molecular methods. First, we investigated the genetic health of 19 gray wolves (Canis lupus) relocated to Isle Royale National Park to restore ecosystem balance. Initial genetic assessments with microsatellites found that these wolves had genetic diversity metrics …

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Synthesis Of Long Oligodeoxynucleotides, Yipeng Yin Jan 2024

Synthesis Of Long Oligodeoxynucleotides, Yipeng Yin

Dissertations, Master's Theses and Master's Reports

Synthetic long oligodeoxynucleotides (ODNs) have found wide applications in diverse fields such as chemical biology, synthetic biology, and genes and genomes synthesis. Those applications leading to a significant demand for their production. However, traditional methods for purifying synthetic ODNs present notable drawbacks, particularly their inability to purify long ODNs, rendering long ODN synthesis challenging. To address these issues, chemical synthesis techniques for long ODNs coupled with non-chromatographic purification methods have been developed. This technology makes the purification of long synthetic ODNs feasible and offers a viable pathway for producing genes and genomes with extensive repeats or stable secondary structures, which …

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