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2004

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Articles 61 - 90 of 124

Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Heterogeneous Chemistry Involving Methanol In Tropospheric Clouds, A. Tabazadeh, Robert J. Yokelson, H. B. Singh, Peter V. Hobbs, J. H. Crawford, L. T. Iraci Mar 2004

Heterogeneous Chemistry Involving Methanol In Tropospheric Clouds, A. Tabazadeh, Robert J. Yokelson, H. B. Singh, Peter V. Hobbs, J. H. Crawford, L. T. Iraci

Chemistry and Biochemistry Faculty Publications

In this report we analyze airborne measurements to suggest that methanol in biomass burning smoke is lost heterogeneously in clouds. When a smoke plume intersected a cumulus cloud during the SAFARI 2000 field project, the observed methanol gas phase concentration rapidly declined. Current understanding of gas and aqueous phase chemistry cannot explain the loss of methanol documented by these measurements. Two plausible heterogeneous reactions are proposed to explain the observed simultaneous loss and production of methanol and formaldehyde, respectively. If the rapid heterogeneous processing of methanol, seen in a cloud impacted by smoke, occurs in more pristine clouds, it could …

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Interaction Between Amino Propeptides Of Type Xi Procollagen Α1 Chains, Julia T. Oxford Mar 2004

Interaction Between Amino Propeptides Of Type Xi Procollagen Α1 Chains, Julia T. Oxford

Biomedical Research Institute Publications and Presentations

Type XI collagen is a quantitatively minor yet essential constituent of the cartilage extracellular matrix. The amino propeptide of the α1 chain remains attached to the rest of the molecule for a longer period of time after synthesis than the other amino propeptides of type XI collagen and has been localized to the surface of thin collagen fibrils. Yeast two-hybrid system was used to demonstrate that a homodimer of α1(XI) amino propeptide (α1(XI)Npp) could form in vivo. Interaction was also confirmed using multi-angle laser light scattering, detecting an absolute weight average molar mass ranging from the size of a monomer …

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Photoreactive Bicyclic Amino Acids As Substrates For Mutant Escherichia Coli Phenylalanyl-Trna Synthetases, Thomas Bentin, Ramin Hamzavi, Jahan Salomonsson, Hervé Roy, Michael Ibba, Peter E. Nielsen Mar 2004

Photoreactive Bicyclic Amino Acids As Substrates For Mutant Escherichia Coli Phenylalanyl-Trna Synthetases, Thomas Bentin, Ramin Hamzavi, Jahan Salomonsson, Hervé Roy, Michael Ibba, Peter E. Nielsen

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Unnatural amino acids carrying reactive groups that can be selectively activated under non-invasive biologically benign conditions are of interest in protein engineering as biological tools for the analysis of protein-protein and protein-nucleic acids interactions. The double ring system phenylalanine analogues benzofuranylalanine and benzotriazolylalanine were synthesized, and their photolability was tested by UV irradiation at 254, 320, and 365 nm. Although both showed photo reactivity, benzofuranylalanine appeared as the most promising compound because this amino acid was activated by UVA (long wavelength) irradiation. These amino acids were also tested for in vitro charging of tRNAPhe and for protein mutagenesis via …

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Differences In Response To Hypoxia In The Three-Spined Stickleback From Lotic And Lentic Localities: Dominance And An Anaerobic Metabolite, L. U. Sneddon, J. Yerbury Mar 2004

Differences In Response To Hypoxia In The Three-Spined Stickleback From Lotic And Lentic Localities: Dominance And An Anaerobic Metabolite, L. U. Sneddon, J. Yerbury

Aquaculture Collection

Dominance hierarchies of the three-spined stickleback Gasterosteus aculeatus from river and pond populations were subjected to hypoxia (20%, range±1%). Under hypoxia, the hierarchies were less stable in terms of rank position and tissue L-lactate was higher in river fish than pond fish under normoxia and hypoxia. Dominant fish gained mass under normoxia but lost mass under hypoxic conditions possibly due to them maintaining high levels of aggression.

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Specific Excision Of The Selenocysteine Trna [Ser]Sec (Trsp) Gene In Mouse Liver Demonstrates An Essential Role Of Selenoproteins In Liver Function, Bradley A. Carlson, Sergey V. Novoselov, Easwari Kumaraswamy, Byeong Jae Lee, Miriam R. Anver, Vadim N. Gladyshev, Dolph L. Hatfield Feb 2004

Specific Excision Of The Selenocysteine Trna [Ser]Sec (Trsp) Gene In Mouse Liver Demonstrates An Essential Role Of Selenoproteins In Liver Function, Bradley A. Carlson, Sergey V. Novoselov, Easwari Kumaraswamy, Byeong Jae Lee, Miriam R. Anver, Vadim N. Gladyshev, Dolph L. Hatfield

Vadim Gladyshev Publications

Selenium is essential in mammalian embryonic development. However, in adults, selenoprotein levels in several organs including liver can be substantially reduced by selenium deficiency without any apparent change in phenotype. To address the role of selenoproteins in liver function, mice homozygous for a floxed allele encoding the selenocysteine (Sec) tRNA [Ser]Sec gene were crossed with transgenic mice carrying the Cre recombinase under the control of the albumin promoter that expresses the recombinase specifically in liver. Recombination was nearly complete in mice 3 weeks of age, whereas liver selenoprotein synthesis was virtually absent, which correlated with the loss of Sec tRNA …

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The Identity Of Proteins Associated With A Small Heat Shock Protein During Heat Stress In Vivo Indicates That These Chaperones Protect A Wide Range Of Cellular Functions, Eman Basha, Garrett J. Lee, Linda A. Breci, Andrew C. Hausrath, Nicole R. Buan, Kim C. Giese, Elizabeth Vierling Feb 2004

The Identity Of Proteins Associated With A Small Heat Shock Protein During Heat Stress In Vivo Indicates That These Chaperones Protect A Wide Range Of Cellular Functions, Eman Basha, Garrett J. Lee, Linda A. Breci, Andrew C. Hausrath, Nicole R. Buan, Kim C. Giese, Elizabeth Vierling

Department of Biochemistry: Faculty Publications

The small heat shock proteins (sHSPs) are a ubiquitous class of ATP-independent chaperones believed to prevent irreversible protein aggregation and to facilitate subsequent protein renaturation in cooperation with ATP-dependent chaperones. Although sHSP chaperone activity has been studied extensively in vitro, understanding the mechanism of sHSP function requires identification of proteins that are sHSP substrates in vivo. We have used both immunoprecipitation and affinity chromatography to recover 42 proteins that specifically interact with Synechocystis Hsp16.6 in vivo during heat treatment. These proteins can all be released from Hsp16.6 by the ATP-dependent activity of DnaK and cochaperones and are heat-labile. Thirteen …

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Methionine Sulfoxide Reduction In Mammals: Characterization Of Methionine-R-Sulfoxide Reductases, Hwa-Young Kim, Vadim Gladyshev Feb 2004

Methionine Sulfoxide Reduction In Mammals: Characterization Of Methionine-R-Sulfoxide Reductases, Hwa-Young Kim, Vadim Gladyshev

Vadim Gladyshev Publications

Methionine residues in proteins are susceptible to oxidation by reactive oxygen species, but can be repaired via reduction of the resulting methionine sulfoxides by methionine-S-sulfoxide reductase (MsrA) and methionine-R-sulfoxide reductase (MsrB). However, the identity of all methionine sulfoxide reductases involved, their cellular locations and relative contributions to the overall pathway are poorly understood. Here, we describe a methionine-R-sulfoxide reduction system in mammals, in which two MsrB homologues were previously described. We found that human and mouse genomes possess three MsrB genes and characterized their protein products, designated MsrB1, MsrB2, and MsrB3. MsrB1 (Selenoprotein R) was present in the cytosol and …

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Mer1p Is A Modular Splicing Factor Whose Function Depends On The Conserved U2 Snrnp Protein Snu17p, Marc Spingola, Javier Armisen, Manuel Ares Feb 2004

Mer1p Is A Modular Splicing Factor Whose Function Depends On The Conserved U2 Snrnp Protein Snu17p, Marc Spingola, Javier Armisen, Manuel Ares

Biology Department Faculty Works

Mer1p activates the splicing of at least three pre‐mRNAs (AMA1, MER2, MER3) during meiosis in the yeast Saccharomyces cerevisiae. We demonstrate that enhancer recognition by Mer1p is separable from Mer1p splicing activation. The C‐terminal KH‐type RNA‐binding domain of Mer1p recognizes introns that contain the Mer1p splicing enhancer, while the N‐terminal domain interacts with the spliceosome and activates splicing. Prior studies have implicated the U1 snRNP and recognition of the 5′ splice site as key elements in Mer1p‐activated splicing. We provide new evidence that Mer1p may also function at later steps of spliceosome assembly. First, Mer1p can activate splicing of introns …

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Tissue-Specific Expression And Developmental Regulation Of Cytochrome B561 Genes In Arabidopsis Thaliana And Raphanus Sativus, Wim Verelst, Jyoti Kapila, Janice De Almeida Engler, Julie M. Stone, Roland Caubergs, Han Asard Feb 2004

Tissue-Specific Expression And Developmental Regulation Of Cytochrome B561 Genes In Arabidopsis Thaliana And Raphanus Sativus, Wim Verelst, Jyoti Kapila, Janice De Almeida Engler, Julie M. Stone, Roland Caubergs, Han Asard

Department of Biochemistry: Faculty Publications

Ascorbate (Asc) is an essential molecule in many aspects of development and stress responses in plants and animals. Cytochromes b561 (cyts b561) are tightly coupled to Asc homeostasis. These proteins are found in mammalian tissues, where they are involved in the regeneration of Asc, serving the synthesis of catecholamine neurotransmitters, and in intestinal iron reduction. Plant genomes encode homologous membrane-associated, Asc-reducible cyts b561. The expression of these proteins in plants, however, has so far not been studied. We have now examined the expression of two Arabidopsis thaliana cyt b561-encoding genes—Artb561-1 and Artb561-2—using relative-quantitative RT-PCR …

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Comprehensive Laboratory Measurements Of Biomass-Burning Emissions: 2. First Intercomparison Of Open-Path Ftir, Ptr-Ms, And Gc-Ms/Fid/Ecd, Ted J. Christian, B. Kleiss, Robert J. Yokelson, R. Holzinger, P. J. Crutzen, Wein Min Hao, T. Shirai, Donald R. Blake Jan 2004

Comprehensive Laboratory Measurements Of Biomass-Burning Emissions: 2. First Intercomparison Of Open-Path Ftir, Ptr-Ms, And Gc-Ms/Fid/Ecd, Ted J. Christian, B. Kleiss, Robert J. Yokelson, R. Holzinger, P. J. Crutzen, Wein Min Hao, T. Shirai, Donald R. Blake

Chemistry and Biochemistry Faculty Publications

No abstract provided.

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Divergence In Non-Cognate Amino Acid Recognition Between Class I And Class Ii Lysyl-Trna Synthetases, Jeffrey D. Levengood, Sandro F. Ataide, Hervé Roy, Michael Ibba Jan 2004

Divergence In Non-Cognate Amino Acid Recognition Between Class I And Class Ii Lysyl-Trna Synthetases, Jeffrey D. Levengood, Sandro F. Ataide, Hervé Roy, Michael Ibba

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Lysine insertion during coded protein synthesis requires lysyl-tRNALys, which is synthesized by lysyl-tRNA synthetase (LysRS). Two unrelated forms of LysRS are known: LysRS2, which is found in eukaryotes, most bacteria, and a few archaea, and LysRS1, which is found in most archaea and a few bacteria. To compare amino acid recognition between the two forms of LysRS, the effects of l-lysine analogues on aminoacylation were investigated. Both enzymes showed stereospecificity toward the l-enantiomer of lysine and discriminated against noncognate amino acids with different R-groups (arginine, ornithine). Lysine analogues containing substitutions at other positions were generally most effective as …

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Appendix C: General Data, Problems In Environmental Science Course (Biology 493), Colby College, Colby Environmental Assessment Team, Colby College Jan 2004

Appendix C: General Data, Problems In Environmental Science Course (Biology 493), Colby College, Colby Environmental Assessment Team, Colby College

Colby College Watershed Study: Togus Pond (2004)

No abstract provided.

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Fall Data Report, Problems In Environmental Science Course (Biology 493), Colby College, Colby Environmental Assessment Team, Colby College Jan 2004

Fall Data Report, Problems In Environmental Science Course (Biology 493), Colby College, Colby Environmental Assessment Team, Colby College

Colby College Watershed Study: Togus Pond (2004)

No abstract provided.

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Total Phosphorus, Colby College, Problems In Environmental Science Course (Biology 493), Colby College, Colby Environmental Assessment Team, Colby College Jan 2004

Total Phosphorus, Colby College, Problems In Environmental Science Course (Biology 493), Colby College, Colby Environmental Assessment Team, Colby College

Colby College Watershed Study: Togus Pond (2004)

No abstract provided.

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An Assessment Of Water Quality, Colby College, Problems In Environmental Science Course (Biology 493), Colby College Jan 2004

An Assessment Of Water Quality, Colby College, Problems In Environmental Science Course (Biology 493), Colby College

Colby College Watershed Study: Togus Pond (2004)

No abstract provided.

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Water Quality Results From Togus Pond, Colby College, Problems In Environmental Science Course (Biology 493), Colby College Jan 2004

Water Quality Results From Togus Pond, Colby College, Problems In Environmental Science Course (Biology 493), Colby College

Colby College Watershed Study: Togus Pond (2004)

No abstract provided.

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A Watershed Analysis Of Togus Pond: Implications For Water Quality And Land Use Management, Colby Environmental Assessment Team, Colby College, Problems In Environmental Science Course (Biology 493), Colby College Jan 2004

A Watershed Analysis Of Togus Pond: Implications For Water Quality And Land Use Management, Colby Environmental Assessment Team, Colby College, Problems In Environmental Science Course (Biology 493), Colby College

Colby College Watershed Study: Togus Pond (2004)

The Colby Environmental Assessment Team (CEAT) investigated the water quality and factors affecting water quality in the surrounding watershed of Togus Pond in Augusta, Maine from June through September 2004. CEAT analyzed several physical, chemical and biological water quality parameters, land use patterns in the watershed, and the impact of residential and commercial development on water quality. Data collected were used to produce models of the watershed that enabled CEAT to identify possible sources of degradation to the current and future water quality of Togus Pond. To obtain a historical perspective, all data collected this summer were compared to data …

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Identification Of Trace Element-Containing Proteins In Genomic Databases, Vadim N. Gladyshev, Gregory V. Kryukov, Dmitri E. Fomenko, Dolph L. Hatfield Jan 2004

Identification Of Trace Element-Containing Proteins In Genomic Databases, Vadim N. Gladyshev, Gregory V. Kryukov, Dmitri E. Fomenko, Dolph L. Hatfield

Vadim Gladyshev Publications

Development of bioinformatics tools provided researchers with the ability to identify full sets of trace element–containing proteins in organisms for which complete genomic sequences are available. Recently, independent bioinformatics methods were used to identify all, or almost all, genes encoding selenocysteine-containing proteins in human, mouse, and Drosophila genomes, characterizing entire selenoproteomes in these organisms. It also should be possible to search for entire sets of other trace element–associated proteins, such as metal-containing proteins, although methods for their identification are still in development.

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Altered Phosphorylation Of [Beta]-Catenin In Glucocorticoid Treated 235-1 Rat Pituitary Tumor Cells, Susie K. Saunders Jan 2004

Altered Phosphorylation Of [Beta]-Catenin In Glucocorticoid Treated 235-1 Rat Pituitary Tumor Cells, Susie K. Saunders

Theses, Dissertations and Capstones

Beta-catenin is an essential cell adhesion and signaling protein, associated with high prolactin levels in rat pituitary tumor cells. It has been shown that phosphorylation affects the location and activity of b-catenin. Glycogen synthase kinase (GSK3-b) is a serine-threonine kinase that phosphorylates b-catenin on N-terminal residues, targeting it for proteasomal degradation. Studies have shown that C-terminal tyrosine phosphorylation decreases the association of b-catenin with cadherin. In 235-1 rat pituitary tumor cells, our lab has shown that the glucocorticoid analog dexamethasone (Dex) decreases the half- life of b-catenin while increasing the activity of GSK3-b. The current study was undertaken to examine …

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Molecular Typing Of Mycobacterial Isolates Cultured From The Tissue Of Inflammatory Bowel Disease (Crohn's Disease) Patients, Leanne M. Adams Jan 2004

Molecular Typing Of Mycobacterial Isolates Cultured From The Tissue Of Inflammatory Bowel Disease (Crohn's Disease) Patients, Leanne M. Adams

Electronic Theses and Dissertations

The role of Mycobacterium avium subsp paratuberculosis (MAP) in the etiology and pathogenesis of inflammatory bowel disease (IBD) including Crohn's Disease (CD), has been investigated. The fastidious characteristics and cross reactivity of MAP with other members in Mycobacteria have produced significant challenges in their detection and identification. In this two year pilot study, an array of three PCR molecular assays based on the detection of sequences from the16S rRNA, IS1245, and IS900 genes, belonging to members of the MAC, have been developed and optimized into a common protocol to be used as a rapid and accurate diagnostic tool regarding M. …

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Extracullular Atp Regulates Il-1beta Release From Microglial Cells Via Purinergic Receptor After In Vitro Trauma, Chengya Liang Jan 2004

Extracullular Atp Regulates Il-1beta Release From Microglial Cells Via Purinergic Receptor After In Vitro Trauma, Chengya Liang

Electronic Theses and Dissertations

Traumatic brain injury (TBI) induces a state of microglialactivation, which includes upregulation of macrophage functions and release inflammatory mediators such as certain inflammatory cytokines. Current literature suggests that interleukin-1Beta is an important cytokine mediator, which is dramatically increased after brain injury. Previous studies indicate that ATP is released by traumatically injured astrocytes and serves as a cell-to-cell mediator through purinergic receptors after in vitro injury. However, the mechanism of interleukin-1Beta release after traumatic brain injury remains poorly defined and is difficult to study using in vivo models. Using an in vitro model for traumatic brain injury (cell strain or stretch), …

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An Investigation Into The Effects Of Structural Isomers Of Salicylic Acid On Propionibacterium Acnes, Ireland Burch Jan 2004

An Investigation Into The Effects Of Structural Isomers Of Salicylic Acid On Propionibacterium Acnes, Ireland Burch

Capstone Research Projects

Acne is caused when the sebaceous glands in the skin are unable to secrete an oily substance, called sebum, onto the surface of the skin. This is because the opening of the follicle is clogged from a mixture of skin cells and sebum. The opening of the follicle becomes plugged when the cells from the lining of the follicle are shed too quickly and clump together. Because Propionibacterium acnes (P. acnes) bacteria feed off sebum, they are then able to grow in the plugged follicle. Acne is caused by the inflammation that results from the chemicals and enzymes produced from …

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Isolation Ofphosphoglucose Isomerase From Peas, Tyler Dubs Jan 2004

Isolation Ofphosphoglucose Isomerase From Peas, Tyler Dubs

Capstone Research Projects

No abstract provided.

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Synergistic Mixtures Of Fungitoxic Monochloro And Dichloro-8-Quinolinols Against Five Fungi / Herman Gershon, Muriel Gershon, & Donald D. Clarke Harding Laboratory, The New York Botanical Garden, Bronx, Ny 10458-5126, Usa; Department Of Chemistry, Fordham University, Bronx, Ny 10458-9993, Usa, Herman Gershon, Muriel Gershon, Donald Dudley Clarke Phd Jan 2004

Synergistic Mixtures Of Fungitoxic Monochloro And Dichloro-8-Quinolinols Against Five Fungi / Herman Gershon, Muriel Gershon, & Donald D. Clarke Harding Laboratory, The New York Botanical Garden, Bronx, Ny 10458-5126, Usa; Department Of Chemistry, Fordham University, Bronx, Ny 10458-9993, Usa, Herman Gershon, Muriel Gershon, Donald Dudley Clarke Phd

Chemistry Faculty Publications

Fourteen mono- and dichloro-8-quinolinols were tested against five fungi (Aspergillus niger, A. oryzae, Myrothecium verrucaria, Trichoderma viride, and Mucor circinelloides) and compared with the fungitoxicity of 8- quinolinol in Yeast Nitrogen Base containing l% D-glucose and 0.088% L-asparagine. All of the compounds were more fungitoxic than 8-quinolinol except for the surprising activity of 8-quinolinol against A. oryzae. Mixtures of the MICs of monochloro- and dichloro-8-quinolinols in which the halogens were in different positions of the quinoline ring showed synergism. Comparable mixtures in which one position of each compound was occupied by the same halogen showed additive activity. In a different …

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Intravenous 2-Deoxy-D-Glucose Injection Rapidly Elevates Levels Of The Phosphorylated Forms Of P44/42 Mitogen-Activated Protein Kinases (Extracellularly Regulated Kinases 1/2) In Rat Hypothalamic Parvicellular Paraventricular Neurons, Arshad Khan, Alan Watts Jan 2004

Intravenous 2-Deoxy-D-Glucose Injection Rapidly Elevates Levels Of The Phosphorylated Forms Of P44/42 Mitogen-Activated Protein Kinases (Extracellularly Regulated Kinases 1/2) In Rat Hypothalamic Parvicellular Paraventricular Neurons, Arshad Khan, Alan Watts

Selected Works Temporary Series

No abstract provided.

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Distributions Of Z-Dna And Nuclear Factor I In Human Chromosome 22: A Model For Coupled Transcriptional Regulation, P. Christoph Champ, Sandor Maurice, Jeff Vargason, Tracy Camp, P. Shing Ho Jan 2004

Distributions Of Z-Dna And Nuclear Factor I In Human Chromosome 22: A Model For Coupled Transcriptional Regulation, P. Christoph Champ, Sandor Maurice, Jeff Vargason, Tracy Camp, P. Shing Ho

Faculty Publications - Department of Biological & Molecular Science

An analysis of the human chromosome 22 genomic sequence shows that both Z-DNA forming regions (ZDRs) and promoter sites for nuclear factor-I (NFI) are correlated with the locations of known and predicted genes across the chromosome and accumulate around the transcriptional start sites of the known genes. Thus, the occurrence of Z-DNA across human genomic sequences mirrors that of a known eukaryotic transcription factor. In addition, 43 of the 383 fully annotated chromosomal genes have ZDRs within 2 nucleosomes upstream of strong NFIs. This suggests a distinct class of human genes that may potentially be transcriptionally regulated by a mechanism …

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Development Of A Gene Transfer System In Clostridium Scindens Vpi 12708, Rashmi Ramasubbaiah Jan 2004

Development Of A Gene Transfer System In Clostridium Scindens Vpi 12708, Rashmi Ramasubbaiah

Masters Theses & Specialist Projects

Clostridium scindens VPI 12708 (previously known as Eubacterium sp. VPI 12708) is a bile-acid dehydroxylating bacterium originally isolated from the feces of a colon cancer patient. Many genes required for bile acid 7-a dehydroxylation are found on a large bile acid inducible operon (bai) that has been extensively studied. However the bai promoter, which directs expression of the bai operon, has yet to be characterized due, in part, to a lack of a functional genetic transfer system for this strain. A spontaneous rifampinresistant Clostridium scindens VPI 12708 mutant was used as a recipient to determine the efficacy of conjugation as …

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Quantitative Non-Isotopic Nitrocellulose Filter Binding Assays: Bacterial Mnsod-Dna Interactions, Joshua D. Czerwinski, David P. Mascotti Jan 2004

Quantitative Non-Isotopic Nitrocellulose Filter Binding Assays: Bacterial Mnsod-Dna Interactions, Joshua D. Czerwinski, David P. Mascotti

Chemistry

No abstract provided.

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Investigating Fluctuating Asymmetry Of The Larval Damselfly, Calopteryx Maculata (Odonata: Calopterigidae), Edward Kelliher Jan 2004

Investigating Fluctuating Asymmetry Of The Larval Damselfly, Calopteryx Maculata (Odonata: Calopterigidae), Edward Kelliher

Undergraduate Review

Fluctuating asymmetry (FA), or subtle random deviations from perfect bilateral symmetry, has recently become a useful tool in allowing researchers to understand more about an organism's health, fitness, developmental stability and environmental stressors. Ultimately, FA studies can be used as an indirect measurement of the quality of an aquatic system over time. We measured and examined the femur segments of the larval damselfly, Calopteryx maculata from sites on the Town, Hockomock, and Salisbury Plain Rivers, of Plymouth County, Massachusetts to determine FA levels. After accounting for measurement error, preliminary results show that variations in symmetry are not correlated to individual …

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Identification Of Phosphorylase Kinase Alpha Subunit Binding Partners In Skeletal Muscle, Soleil Archila Jan 2004

Identification Of Phosphorylase Kinase Alpha Subunit Binding Partners In Skeletal Muscle, Soleil Archila

Masters Theses & Specialist Projects

IDENTIFICATION OF PHOSPHORYLASE KINASE ALPHA SUBUNIT BINDING PARTNERS IN SKELETAL MUSCLE Soleil Archila August 2004 67 Pages Directed by: Nancy A. Rice, Sigrid Jacobshagen, and Claire A. Rinehart Department of Biology Western Kentucky University Phosphorylase kinase (PhK) integrates neural, hormonal, and metabolic signals in skeletal muscle to tightly regulate glycogen breakdown and energy production. Structurally, PhK is among the largest and most complex kinases known with a stoichiometry of (αβγδ)4 and a mass of 1.3x106 Da. The catalytic γ subunit is allosterically controlled through alterations in quaternary structure initiated by the regulatory α, β and δ subunits. In this study …

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