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Biochemistry, Biophysics, and Structural Biology Commons™
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Articles 1 - 14 of 14
Full-Text Articles in Biochemistry, Biophysics, and Structural Biology
Mechanism Of Decarboxylation Of Glycine And Glycolate By Isolated Soybean Cells, David J. Oliver
Mechanism Of Decarboxylation Of Glycine And Glycolate By Isolated Soybean Cells, David J. Oliver
David J. Oliver
Isolated soybean leaf mesophyll cells decarboxylated exogenously added [1-14C]glycolate and [1-14C]glycine in the dark. The rate of CO2 release from glycine was inhibited over 90% by isonicotinic acid hydrazide and about 80% by KCN, two inhibitors of the glycine to serine plus CO2 reaction. The release of CO2 from glycolate was inhibited by less than 50% under the same conditions. This indicates that about 50% of the CO2 released from glycolate occurred at a site other than the glycine to serine reaction. The sensitivity of this alternative site of CO2 release to an inhibitor of glycolate oxidase (methyl-2-hydroxy-3-butynoate) but not …
The Isolation, Purification, And Characterization Of A Bacterial Enzyme Catalyzing The Reductive Cleavage Of Anthracycline Glycosides, Mark W. Collison
The Isolation, Purification, And Characterization Of A Bacterial Enzyme Catalyzing The Reductive Cleavage Of Anthracycline Glycosides, Mark W. Collison
Masters Theses
No abstract provided.
The Synthesis And Evaluation Of The Potential Antineoplastic Activity Of The Semicarbazone And The Thiosemicarbazone Derivatives Of The Di-2-Pyridyl Ketone And The 1,8-Diazafluorenone Ring Systems, Adriene Y. Principe
Chemistry & Biochemistry Theses & Dissertations
A number of' a-(N)-heterocyclic thiosemicarbazones have been synthesized and evaluated for potential antineoplastic activity since Brockman and colleagues2 reported the antineoplastic activity of' 2-f'ormylpyridine thiosemicarbazone (1). The primary objective of' this research project has been the synthesis and characterization of' the semicarbazone and the thiosemicarbazone derivatives of' the di-2-pyridyl ketone, (9-a) and (9-b), and the 1,8-diazaf'luorenone, (10-a) and (10-b), ring systems, as well as the evaluation of' these compounds for both in vitro and in vivo antineoplastic activity against Ehrlich ascites tumor cells in mice. These studies have suggested that di-2-pyridyl ketone thiosemicarbazone (9-b) is as effective as 2-formylpyridine …
Heating-Effect Minimization In Dye Lasers, Kenneth Lee Matheson, James M. Thorne
Heating-Effect Minimization In Dye Lasers, Kenneth Lee Matheson, James M. Thorne
Faculty Publications
Optical pumping of dye lasers deposits heat nonuniformly in the active medium. Because the refractive index of a liquid or gas is a function of temperature, gradients are created which refract the laser beam. This effect is sometimes serious enough to quench laser action. In this paper, we evaluate the effects caused by optical pumping with a coaxial flashlamp and suggest ultraviolet absorbers and wavelength shifters as materials for minimizing the problem
Ascorbic Acid, Lipid Peroxidation, And Aging, Brian Evan Leibovitz
Ascorbic Acid, Lipid Peroxidation, And Aging, Brian Evan Leibovitz
Dissertations and Theses
The role of ascorbic acid with regard to lipid peroxidation and aging has been examined. A thorough literature analysis indicates that free radical-induced lipid peroxidation is a plausible biochemical explanation for aging. Lipid peroxidation causes cellular damage due to altered enzyme activities, error-prone nucleic acid metabolism, and membrane dysfunction, as well as the accumulation of aging pigments in the lysomes. Ascorbic acid, a water soluble free radical quencher, was examined with regard to carbon tetra-chloride-induced lipid peroxidation and in vivo aging. Carbon tetrachloride, a well-known free radical inducer, caused marked increases in the ration of oxidized/reduced vitamin C only …
Cow Brain Glutaminase: Purification, Characterization, And Mechanism Of Action, Fulung John Chiu
Cow Brain Glutaminase: Purification, Characterization, And Mechanism Of Action, Fulung John Chiu
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
A simple, quick, and sensitive radiometric assay for glutaminase has been established. This assay uses L-(U-14C)-glutamine as the substrate and measures the 14CO2 produced when the reaction is coupled to glutamate decarboxylase. An efficient purification procedure for cow brain glutaminase has also been developed; the yield is 12%. Steps include acetone extraction, ammonium sulfate fractionation, calcium phosphate gel treatment, and differential gel filtration on Sepharose 4B. The final preparation has a specific activity of 385 μmole/min/mg and shows a single protein band on polyacrylamide gel electrophoresis in sodium dodecyl sulfate; this band corresponds to a …
Fractionation Of Ribosomal Proteins By Affinity Chromatography, Corazon E. Victoria
Fractionation Of Ribosomal Proteins By Affinity Chromatography, Corazon E. Victoria
Masters Theses
No abstract provided.
Steriodogenesis In Mouse Preimplantation Embryos, Marcia E. Kendall
Steriodogenesis In Mouse Preimplantation Embryos, Marcia E. Kendall
Morehead State Theses and Dissertations
A thesis presented to the faculty of the School of Sciences and Mathematics at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science in Biology by Marcia E. Kendall February 8, 1979.
The Purification, Properties And Chemical Kinetics Of Cathepsin B1, Andrew Stanley Bajkowski
The Purification, Properties And Chemical Kinetics Of Cathepsin B1, Andrew Stanley Bajkowski
Master's Theses
No abstract provided.
Photoelectric Properties And Detection Of The Aromatic Carcinogens Benzo[A]Pyrene And Dimethylbenzanthracene, William A. Houle, Hugh M. Brown, O. Hayes Griffith
Photoelectric Properties And Detection Of The Aromatic Carcinogens Benzo[A]Pyrene And Dimethylbenzanthracene, William A. Houle, Hugh M. Brown, O. Hayes Griffith
Department of Biochemistry: Faculty Publications
The absolute photoelectron quantum yield spectra for benzo[ajpyrene and dimethylbenzanthracene are presented in the wavelength range 180-230 nm. These polycyclic aromatic carcinogens have photoelectron quantum yields of approximately 2 X 10-3 electrons per incident photon at 180 nm. The quantum yields fall off quickly and monotonically at wavelengths longer than 210 nm (5.9 eV). Threshold values for benzo[a]pyrene and dimethylbenzanthracene are 5.25 ± 0.06 eV and 5.27 ± 0.04 eV, respectively. The photoelectron quantum yields of benzo[a]pyrene and dimethylben-zanthracene are several orders of magnitude greater than typical components of biological membranes (amino acids, phospholipids, and polysaccharides). Preliminary micrographs of …
Potential Use Of Hydroelectric Facilities For Manipulating The Fertility Of Lake Mead, Larry J. Paulson, John R. Baker, James E. Deacon
Potential Use Of Hydroelectric Facilities For Manipulating The Fertility Of Lake Mead, Larry J. Paulson, John R. Baker, James E. Deacon
Publications (WR)
Analysis of historical nutrient data for Lake Mead indicates that the fertility of the reservoir has decreased which may be the cause for a corresponding decline in the largemouth bass population. However, it appears that fertility can be manipulated by altering the operation of the dam. The depletion of nutrients in the euphotic zone by phytoplankton and subsequent accumulation in the hypolimnion during summer and fall provide a natural nutrient gradient from which water of varying fertility can be drawn for discharge. This combined with alterations in the depth or seasonal pattern of discharge can possibly be used to enhance …
Synthesis Of 2-Aminomorphine And 2-Aminocodeine. Reduction Of Aromatic Nitro Groups With Formamidinesulfinic Acid. / Nithiananda Chatterjie, Arlene Minar And Donald D. Clarke New York State Institute For Basic Research In Mental Retardation, Staten Island, Ny 10314, Nithiananda Chatterjie, Arlene Minar, Donald Dudley Clarke Phd
Synthesis Of 2-Aminomorphine And 2-Aminocodeine. Reduction Of Aromatic Nitro Groups With Formamidinesulfinic Acid. / Nithiananda Chatterjie, Arlene Minar And Donald D. Clarke New York State Institute For Basic Research In Mental Retardation, Staten Island, Ny 10314, Nithiananda Chatterjie, Arlene Minar, Donald Dudley Clarke Phd
Chemistry Faculty Publications
Our interest in the stereoselective reduction of the carbonyl groups of various oxymorphone derivatives with formamidinesulfinic acid (FSA) led to a result indicating that dihydrocodeinone underwent ring-oxygen scission under the conditions of reduction with this reagent. This observation4 has prompted interest and speculation as to the scope and mechanism of this reaction by others, as well as ourselves
An Investigation Of Possible Inhibitors For D-Amino Acid Oxidase, Sue Ellen Delos
An Investigation Of Possible Inhibitors For D-Amino Acid Oxidase, Sue Ellen Delos
Dissertations, Theses, and Masters Projects
No abstract provided.
Pulling The Wool Off, Department Of Agriculture, Western Australia
Pulling The Wool Off, Department Of Agriculture, Western Australia
Journal of the Department of Agriculture, Western Australia, Series 4
The Department of Agriculture has begun a three year project to study the potential for "chemical shearing" or, more correctly, biological defleecing.