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Articles 181 - 207 of 207

Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Fidelity Of Dna Polymerase In Vitro, Yannacos C. Philopoulos Aug 1982

Fidelity Of Dna Polymerase In Vitro, Yannacos C. Philopoulos

Dissertations

The fidelity of DNA Polymerase I, isolated from B. licheniformis and B. stearothermophilus, was tested in the presence of carcinogens and anticancer compounds. All of these compounds inhibited (62.4-99.2%) the synthesis of DNA using Poly(dA-dT) as a template. The compounds were inhibitory at all concentration levels tested, 0.01, 1.0 and 100 mM. The fidelity of the enzyme appeared to be enhanced by the presence of these compounds, as indicated by misincorporation (50-70%), that is, the incorporation of incorrect nucleotide. Misincorporation in the presence of either carcinogens or anticancer compounds was less than that of control. The results from both organisms …


Elastin Content Of The Aorta From The Quantitation Of The Desmosines, H. Patrick Covault Jan 1982

Elastin Content Of The Aorta From The Quantitation Of The Desmosines, H. Patrick Covault

Dissertations

No abstract provided.


The Uptake And Release Of Label From Endocytosed 35so4-Labeled Proteins In Cultured Mouse Peritoneal Macrophages, Susan Patterson Buktenica Jan 1982

The Uptake And Release Of Label From Endocytosed 35so4-Labeled Proteins In Cultured Mouse Peritoneal Macrophages, Susan Patterson Buktenica

Dissertations

No abstract provided.


The Effects Of Maternal Protein Deficiency On The Development Of Synaptic Plasma Membranes In Rat Offspring, Duane M. Smith Jan 1982

The Effects Of Maternal Protein Deficiency On The Development Of Synaptic Plasma Membranes In Rat Offspring, Duane M. Smith

Dissertations

No abstract provided.


Iodothyronine 5'-Deiodinase: Solubilization, Partial Purification, And Characterization Of A Thyroxine Deiodinase Extracted From Rat Liver Membranes, Samuel C. Powell Jan 1982

Iodothyronine 5'-Deiodinase: Solubilization, Partial Purification, And Characterization Of A Thyroxine Deiodinase Extracted From Rat Liver Membranes, Samuel C. Powell

Dissertations

No abstract provided.


A Microbial Reductive Conversion Of The Steffimycin Chromophore: Chemical And Mechanistic Studies On A Soluble, Nadhdependent, Nonflavin-Containing Reductase From Streptomyces Steffisburgensis, John Rollin Hofstetter Dec 1981

A Microbial Reductive Conversion Of The Steffimycin Chromophore: Chemical And Mechanistic Studies On A Soluble, Nadhdependent, Nonflavin-Containing Reductase From Streptomyces Steffisburgensis, John Rollin Hofstetter

Dissertations

An understanding of a microbial reduction of the anthracycline antibiotic, steffimycin (1) was approached from two directions. First, the NADH-dependent enzyme responsible for the reduction was purified from the Streptomyces steffisburgensis cytosol and characterized. Second, information was obtained on the reduction and oxidation of 1.

The enzyme was a single polypeptide chain, acidic, and stable in solution. It had a molecular weight of 80,000 and no prosthetic groups.

The 5-step purification process resulted in greater than 1,000-fold purification and a 9% yield of the homogenous enzyme from extracts of S. steffisburgensis cells.

The enzyme reduced 1 by a two-electron mechanism …


Neurochemical Studies On The Metabolism And Effects Of Catecholic Isoquinolines, T. C. Origitano Jan 1981

Neurochemical Studies On The Metabolism And Effects Of Catecholic Isoquinolines, T. C. Origitano

Dissertations

No abstract provided.


Electron Spin Resonance Studies Of Membrane Fluidity Of Normal And Malignant Human Neural Tissues, Robert P. Wersto Jan 1981

Electron Spin Resonance Studies Of Membrane Fluidity Of Normal And Malignant Human Neural Tissues, Robert P. Wersto

Dissertations

No abstract provided.


Thermodynamics Of Association And Catalysis Of N-Acyl-Peptidyl-Phenylalanine Amide Substrates By Chymotrypsin, John Paul Huff Jan 1981

Thermodynamics Of Association And Catalysis Of N-Acyl-Peptidyl-Phenylalanine Amide Substrates By Chymotrypsin, John Paul Huff

Dissertations

No abstract provided.


Acetoacetate Decarboxylase Activity Of Fraction V Human Serum Albumin, Robert Richard Rosecrans Jan 1980

Acetoacetate Decarboxylase Activity Of Fraction V Human Serum Albumin, Robert Richard Rosecrans

Dissertations

No abstract provided.


The Role Of Skin Temperature In The Control Of Sweating In Man, Thomas V. Mccaffrey Jan 1980

The Role Of Skin Temperature In The Control Of Sweating In Man, Thomas V. Mccaffrey

Dissertations

No abstract provided.


Immuno- And Bioassayable Extrapituitary Growth Hormone And Thyroid Stimulating Hormone From Discrete Areas Of The Central Nervous System, Sally Ann Hojvat Jan 1980

Immuno- And Bioassayable Extrapituitary Growth Hormone And Thyroid Stimulating Hormone From Discrete Areas Of The Central Nervous System, Sally Ann Hojvat

Dissertations

No abstract provided.


Associations Of N-Acyl Amino Aldehyde Analogs Of Specific Substrates To The Active Site Of Chymotrypsin, William P. Kennedy Jan 1980

Associations Of N-Acyl Amino Aldehyde Analogs Of Specific Substrates To The Active Site Of Chymotrypsin, William P. Kennedy

Dissertations

No abstract provided.


Dna Replication In Mesophilic And Thermophilic Bacteria, Pawan K. Gupta Dec 1978

Dna Replication In Mesophilic And Thermophilic Bacteria, Pawan K. Gupta

Dissertations

No abstract provided.


The Interaction Of Thioxanthenones With Nucleic Acids, Laszlo Bodoni Jan 1978

The Interaction Of Thioxanthenones With Nucleic Acids, Laszlo Bodoni

Dissertations

No abstract provided.


The Metabolism Of Linolenic Acid In Developing Rat Brain, Barney E. Dwyer Jan 1978

The Metabolism Of Linolenic Acid In Developing Rat Brain, Barney E. Dwyer

Dissertations

No abstract provided.


Aluminum Metabolism In Health And Disease, Jay Eliot Gorsky Jan 1978

Aluminum Metabolism In Health And Disease, Jay Eliot Gorsky

Dissertations

No abstract provided.


Evidence For Chromium-Insulin Interaction, Ronald Belusko Jan 1977

Evidence For Chromium-Insulin Interaction, Ronald Belusko

Dissertations

No abstract provided.


Changes In Morphology And Protein Content Of Neurons And Glia Of The Developing Hamster Cerebral Cortex, Thomas E. Durica Jan 1977

Changes In Morphology And Protein Content Of Neurons And Glia Of The Developing Hamster Cerebral Cortex, Thomas E. Durica

Dissertations

No abstract provided.


Studies On The Mechanism Of Inhibition Of Rna Synthesis By Template Inactivators, Nikos Panayotatos Jan 1977

Studies On The Mechanism Of Inhibition Of Rna Synthesis By Template Inactivators, Nikos Panayotatos

Dissertations

No abstract provided.


The Interaction Of Phenanthridinium Derivatives With Nucleic Acids, Antonis Kindelis Jan 1976

The Interaction Of Phenanthridinium Derivatives With Nucleic Acids, Antonis Kindelis

Dissertations

No abstract provided.


Interaction Of Lung Surfactant Lipids With Proteins Of The Alveolar Space, Peter Pagast Jan 1975

Interaction Of Lung Surfactant Lipids With Proteins Of The Alveolar Space, Peter Pagast

Dissertations

No abstract provided.


Studies Of The Effects Of Solvent Composition On The Association-Dissociation Of Oxyhemerythrin As Determined By Analytical Gel Chromatography, Kim Hock Tan Jan 1973

Studies Of The Effects Of Solvent Composition On The Association-Dissociation Of Oxyhemerythrin As Determined By Analytical Gel Chromatography, Kim Hock Tan

Dissertations

No abstract provided.


Polysomes From Mesophilic And Thermophilic Bacteria, Philip Y. Shen Dec 1972

Polysomes From Mesophilic And Thermophilic Bacteria, Philip Y. Shen

Dissertations

No abstract provided.


Purification And Properties Of Phenylalanine Transfer Rna From Bacillus Licheniformis And Bacillus Stearothermophilus, Cynthia R.Y. Yang Apr 1972

Purification And Properties Of Phenylalanine Transfer Rna From Bacillus Licheniformis And Bacillus Stearothermophilus, Cynthia R.Y. Yang

Dissertations

No abstract provided.


A Study Of Glycosphingolipids In Aorta, Blood And Spleen And The Effects Of Atherogenic Diets On These Lipids In Rabbits, Eric Coles Apr 1971

A Study Of Glycosphingolipids In Aorta, Blood And Spleen And The Effects Of Atherogenic Diets On These Lipids In Rabbits, Eric Coles

Dissertations

No abstract provided.


Purification And Properties Of Dna Polymerase From Bacillus Licheniformis And Bacillus Stearothermophilus, Bruce A. Roe Dec 1970

Purification And Properties Of Dna Polymerase From Bacillus Licheniformis And Bacillus Stearothermophilus, Bruce A. Roe

Dissertations

No abstract provided.