Poultry or Avian Science Commons^™

Effect Of Heat Stress On Jejunal Epithelial Barrier Integrity In Broilers Divergently Selected For High- And Low-Water Efficiency, Elizabeth S. Greene, Sara Orlowski, Sami Dridi

Poultry Science Faculty Publications and Presentations

Drought and water scarcity, exacerbated by global warming, are enormous threats to global food sustainability and security. Poultry, in particular, are highly impacted by adverse environmental stressors. As nutrient absorption and intestinal integrity are critical for growth and performance, understanding the impact on the broiler gastrointestinal tract is highly relevant. Here, we examined the effect of chronic cyclic heat stress (HS) on the jejunal expression profile of tight-junction, gap-junction, adherens, and desmosome genes in the 4th generation of broiler lines divergently selected for low (LWE)- and high-water efficiency (HWE). Male HWE and LWE broilers (n = 240/line) were allotted to …

Incidence Of Bacterial Chondronecrosis With Osteomyelitis Lameness In Commercial Duck Flocks In Mojokerto, Indonesia, Andi Asnayanti, Siti Azizah, Anif Mukaromah Wati, Ahmad Ridwan, Ahmad Arman Dahlan, Dinda Rosalita, Anh Dang Trieu Do, Adnan Alrubaye

Poultry Science Faculty Publications and Presentations

Bacterial Chondronecrosis with Osteomyelitis (BCO) lameness is an infection of opportunistic bacteria in the structural skeletal bones impacting multiple animal species, particularly poultry species. BCO lameness results in significant financial losses to industrial poultry production and increases the risk of foodborne illnesses, posing a major threat to consumers' food safety. As BCO lameness is an inherent risk of fast body weight gain in poultry species, especially broiler chickens, abundant studies have been conducted in broilers and turkeys. Nevertheless, BCO lameness incidence in ducks remains elusive. Thus, this is the first survey investigating the prevalence of BCO lameness cases in ducks. …

Characterization Of Water Intake And Water Conversion Ratio For Male And Female Broilers From Four Commercial Broiler Lines Reared To Eight Weeks Of Age, J.Z. Hiltz, C.W. Maynard, T.W. Tabler, M.A. Maquesda, K.M. Shafer, K.B. Nelson, M.T. Kidd, N.B. Anthony, S.K. Orlowski-Workman

Poultry Science Faculty Publications and Presentations

Increased interest and investigation into the sustainability of broiler production has led to a need for reliable and repeatable means to measure water intake and its conversion rate into salable meat. Therefore, a study was conducted to characterize the water intake and water conversion ratio (WCR) of male and female broilers from four modern broiler strains. Two of these lines represented fast growing broiler strains (FGB A and FGB B) targeting a small bird market and two represented high yielding broiler strains (HYB A and HYB B). Three replicates of 25 sexed broilers from each line were placed into 24 …

Effect Of Chronic Heat Stress On Duodenal Epithelial Barrier Integrity In Low- And High-Water-Efficient Broiler Chickens, Lulu Liu, Elizabeth S. Greene, Brooklee Roach, Sara Orlowski, Sami Dridi

Poultry Science Faculty Publications and Presentations

Heat stress (HS) has long posed a significant challenge to the poultry industry due to its adverse effects, such as depressed feed intake, decreased growth performance, and increased water consumption. Water efficiency (WE, conversion of water intake into body weight gain), although often neglected, is a key economic and production trait that is significantly affected by HS. Recently, we selected two broiler lines for high WE (HWE) and low WE (LWE) and showed a differential hypothalamic expression of genes involved in water homeostasis regulation. As the gut also plays a significant role in water absorption, the present study aimed to …

Comparison Of Relative Calcium Bioavailability Based On Bone Ash And Apparent Ileal Ca Digestibility In Broiler Chickens, R. L. Drysdale, B. W. Parsons

Poultry Science Faculty Publications and Presentations

Two experiments were conducted to determine relative Ca bioavailability based on bone ash and apparent ileal digestibility (AID) of Ca in reagent-grade calcium carbonate (RCaCO3) and RCaCO3 + phytic acid in commercial broiler chickens. In Experiment 1, relative Ca bioavailability of RCaCO3 and RCaCO3 + phytic acid was determined in ad libitum-fed broiler chickens using the slope-ratio method. Dietary treatments were corn-soybean meal-based diets. Diet 1 was Ca-deficient (0.30 % Ca; 0.45 % non-phytate P), while diets 2 through 5 were the same as diet 1, except that 2 increasing levels of RCaCO3 or RCaCO3 + phytic acid were added …

Effects Of Heat Stress And Depressed Feed Intake On The Cecal And Ileal Microbiota Of Broiler Chickens, Philip M. Campos, Elizabeth S. Greene, Monika Proszkowiec-Weglarz, Sami Dridi

Poultry Science Faculty Publications and Presentations

Chronic heat stress (HS) is known to depress feed intake (FI) and growth in broiler chickens, and may alter the gut microbiota and health, however, the amplitude and duration of each (HS and depressed FI) effect on gut microbiota is still unclear. This study aimed to determine the effects of chronic and acute HS on the cecal and ileal microbiota profiles. Broilers were subjected to thermoneutral (TN, 23 degrees C) or chronic cyclic HS (CHS, 8 h/d, 35 degrees C) condition from d29-42, and one group to acute HS (AHS, 2 h, 35 degrees C) at d42. To distinguish between …

Smart Search Results For Growth And Economic Performance Comparison Of Twenty-Year-Old ... Refine Results For Arkansas Net Energy: A Productive Energy System Sensitive To Broiler Performance And Body Composition Arkansas Net Energy: A Productive Energy System Sensitive To Broiler Performance And Body Composition 21 Of 94 Arkansas Net Energy: A Productive Energy System Sensitive To Broiler Performance And Body Composition, Diego A. Martinez, Nawin Suesuttajit, Cole Umberson, Craig N. Coon

Poultry Science Faculty Publications and Presentations

This study determined the sensitivity of productive energy (PE; Arkansas Net Energy) to performance and body composition and developed predictive models based on PE. Chicks (2,148 day-old ones) were placed in 96 pens and assigned to one of 12 dietary treatments, including varying concentrations of total digestible amino acids (TDAA), digestible CP (dCP), digestible fat (dFat), digestible starch, and non-starch polysaccharides (NSP). Eight replications per treatment, one per block, were used, being the block consecutive groups of birds weekly starting the seven-day experimental period. In each block, birds were control fed to meet similar ME intake, placed in calorimetry chambers …

Productive Energy, As Determined By Arkansas Net Energy, Predicts Broiler Processing Weights, Carcass Market Value, And Gross Profit, Diego A. Martinez, Nawin Suesuttajit, Cole Umberson, Craig N. Coon

Poultry Science Faculty Publications and Presentations

This study assessed the sensitivity of Productive Energy (PE) to breast meat production, feed efficiency, and broiler economics, developed models for processing traits and economics, and examined economics dynamics. The experimental design involved 96 pens, 12 per block and eight blocks, with each pen within block receiving one of 12 dietary treatments for one week. Treatment diets, varying in amino acid concentrations, starch, and energy densities, were control-fed. The weights of processing body parts and net energy for gain (NEg) were determined with Dual-Energy X-Ray Absorptiometry, and fed heat production, fasting heat production (FHP), and net energy for maintenance (NEm) …

Effects Of Light Wavelength On Broiler Performance, Blood Cell Profiles, Stress Levels, And Tibiotarsi Morphology, Angela Perretti, Victor J. Oyeniran, Jaelen M. Cherry, Rosemarry H. Whittle, Zachary Grider, Alexander H. Nelson, Seong W. Kang, Gisela F. Erf, Shawna L. Wiemer

Poultry Science Faculty Publications and Presentations

Lighting influences broiler production, health, and behavior. The objective of this study was to examine the effects of three light wavelengths (White [350-780 nm], Blue [450 nm], and Green [560 nm]) on broiler production, activity, fear, stress, blood cell profiles, and tibiotarsi (tibia) morphology. Day-of-hatch male broiler chicks (N = 600) were housed in pens (N = 12) with one lighting treatment for 42 days. Body weight and feed consumption were recorded on day (D) 0, 14, 28, and 42, and the feed conversion ratio was calculated. The Tonic Immobility test was used to assess the latency (seconds) to right …

Genetic And Environmental Factors Contributing To The Pathogenesis Of Vertebral Osteomyelitis Caused By Enterococcus Cecorum In Broiler Chicken, Ummu Syauqah Al Musyahadah, Andi Asnayanti, Anh Dang Trieu Do, Adnan Alrubaye

Poultry Science Faculty Publications and Presentations

Enterococcus cecorum (EC) is the primary cause of Vertebral Osteomyelitis (VO) in poultry, characterized by a hock-sitting posture with legs extended forward. The disease begins with intestinal colonization, where pathogenic strains of E. cecorum establish themselves in the gut within the first week of a bird's lifecycle and translocate to the bloodstream later, reaching the organs, which finally infect the birds' Free Thoracic Vertebrae (FTV). Several factors contribute to the initiation and progression of the disease. Environmental factors such as heat stress, high blood sugar levels, prior infections, overcrowding, and rapid growth conditions weaken immunity and stimulate the emergence of …

The Effect Of Feed And Water Provision Strategies On Broiler Breeder Pullet Performance And Welfare, Allison D. Weaver, Lisa R. Bielke, Ramon D. Malheiros, Sara K. Orlowski, Allison N. Pullin

Poultry Science Faculty Publications and Presentations

Feed restriction is common in the broiler breeder industry to optimize health and reproduction. However, this practice has been associated with increased drinking behavior, leading to water spillage, higher litter moisture, and footpad lesions. Consequently, parts of the industry have adopted water restriction protocols. This study aimed to evaluate how different combinations of feed and water restriction affected drinking behavior, welfare, and performance indicators in broiler breeder pullets. At 1 day of age, 960 Cobb 500 FF pullets (Gallus gallus domesticus) were randomly allocated to one of four treatments: skip-a-day feeding with ad libitum water (SAD + ADLIB), every-day feeding …

Feasibility Of Applying Bacillus Amyloliquefaciens-Derived Solid State Fermentation Products Into The Hatch Cabinet Environment As A Method To Mitigate The Microbial Bloom During The Hatching Phase Authors, Mitchell C. Rowland, Lucas E. Graham, Callie Selby, Aaron J. Forga, Billy M. Hargis, Christine N. Voung, Graham Danielle

Poultry Science Faculty Publications and Presentations

Formaldehyde has been used to control microbial contamination in commercial hatch cabinets. The purpose of the present study was to investigate the effects of spray application of Bacillus amyloliquefaciens solid state fermentation products on the microbial load in the hatch cabinet, pioneer colonization of the gastrointestinal tract (GIT), and early performance compared to formaldehyde fumigation. An environmental challenge model was used to simulate the microbial bloom to compare the application of two B. amyloliquefaciens strains (MCR002, MCR009) to formaldehyde fumigation. Groups included 1) non-challenged control (NC), 2) challenged with pathogen mix (PM) containing Escherichia coli, Enterococcus faecalis, and Staphylococcus aureus …

Evaluation Of The Incremental Effect Of Fasting Length On Ph, Select Cecal Microbial Groups, Cecal Volatile Fatty Acid Concentrations, And Secretory Iga Excretion In Roosters, B.W. Parsons, M.R. Bedford, C.L. Wyatt

Poultry Science Faculty Publications and Presentations

Two experiments were conducted to evaluate the effect of incremental fasting time on the gastrointestinal tract in chickens. Adult White Leghorn roosters with intact ceca were provided a nutrient-adequate corn-soybean meal diet ad libitum for 3 weeks. Prior to initiation of the experimental phase, ad libitum feed intake was recorded for 8 h and immediately after the fasting period commenced. In Experiment 1, roosters were fasted for either 0, 3, 6, 9, 12, 16, or 24 h. At each time point birds were euthanized and pH in the crop, gizzard, and ceca were recorded and cecal contents were collected to …

Assessing The Impact Of Spraying An E. Faecium Probiotic At Hatch And Supplementing Feed With A Triple-Strain Bacillus-Based Additive On Bco Lameness Incidence In Broiler Chickens, Khawla Alharbi, Anh Dang Trieu Do, Abdulaziz Alqahtani, Ruvindu Perera, Alexa Thomas, Antoine Meuter, Adnan Ali Khalaf

Poultry Science Faculty Publications and Presentations

Bacterial chondronecrosis with osteomyelitis (BCO) is a major cause of lameness in broiler chickens. This condition arises when bacteria from the gastrointestinal or aerosol tract migrate to infect bone microfractures, often exacerbated by rapid growth, reduced blood flow, and mechanical stress. As concerns about antibiotic resistance grow, probiotics have gained attention for their potential to improve gut health and reduce systemic bacterial load. This study evaluated the efficacy of a probiotic program comprising an Enterococcus faecium-based spray (2 x 10(9) CFU/bird at hatch) and a triple-strain Bacillus-based feed additive (B. subtilis 597, B. subtilis 600, and B. amyloliquefaciens 516 at …

Judgment Bias, Fear, And Stress Responses Of Red Junglefowl And Athens Canadian Random Bred Chickens, Victor J. Oyeniran, Rosemary H. Whittle, Sara K. Orlowski, Shawna L. Weimer

Poultry Science Faculty Publications and Presentations

Understanding how poultry perceive and interpret their environment is essential to enhancing their welfare. Animal welfare science relies on measures of the behavioral and physiological components of affective states (positive and negative) as welfare indicators. There has been growing interest in using the judgment bias test (JBT) to study birds' affective states by assessing their responses to ambiguous cues. The objective of this study was to investigate the affective state of two chicken breeds with different evolutionary histories: the Red Junglefowl (RJF), the primary ancestor of modern chickens, and the Athens Canadian Random Bred (ACRB), a rustic domesticated breed, using …

Genetic Factors Of Campylobacter Jejuni Required For Its Interactions With Free-Living Amoeba, Deepti Pranay Samarth, Asim Z. Abbasi, Young Min Kwon

Poultry Science Faculty Publications and Presentations

Acanthamoeba, a free-living amoeba ubiquitous in environmental water, has been considered as the environmental reservoir of certain bacterial pathogens, including Campylobacter jejuni, an intracellular human pathogen causing self-limiting gastroenteritis. Acanthamoeba-C. jejuni interaction mechanisms may help clarify how the otherwise fastidious bacterium C. jejuni survives in environmental waters. In this study, we constructed single deletion mutants of C. jejuni strain 81-176 for the 10 selected genes (motAB, ciaB, kpsE, virB11, cheY, flaAB, cstII, docB, sodB, and cadF) previously shown to be important for the interaction (invasion and intracellular survival) of C. jejuni with mammalian hosts. We used a modified gentamicin protection …

Effect Of Heat Stress On Ileal Epithelial Barrier Integrity In Broilers Divergently Selected For High- And Low-Water Efficiency, Elizabeth S. Greene, Brooklee Roach, Maria Fernandez Cuadrado, Sara Orlowski, Sami Dridi

Poultry Science Faculty Publications and Presentations

Water scarcity and rising global temperatures are two of the greatest current and future threats to poultry sustainability. Therefore, selection for water efficiency (WE) and heat resilience are of vital importance. Additionally, intestinal integrity is of critical importance under challenging conditions to maintain nutrient absorption and therefore, growth and performance of broilers. Here, we examined the effect of chronic cyclic heat stress (HS) on the ileal expression profile of tight-junction, gap-junction, adherens, and desmosome genes in the fourth generation of divergently selected low (LWE)- and high water efficient (HWE)-chicken lines. LWE birds exhibited higher levels of gut permeability, regardless of …

Evaluation Of The Effect Of In Ovo Applied Bifidobacteria And Lactic Acid Bacteria On Enteric Colonization By Hatchery-Associated Opportunistic Pathogens And Early Performance In Broiler Chickens, Mitchell C. Rowland, Aaron J. Forga, James Higuita, Makenly E. Coles, Billy M. Hargis, Christine N. Vuong, Danielle Graham

Poultry Science Faculty Publications and Presentations

Probiotics have been used to promote pioneer colonization by beneficial bacteria in poultry. The purpose of the present study (four trials) was to determine if an in ovo injection with Bifidobacterium saeculare (B2-2, B3-4) and/or lactic acid bacteria (LAB18, LAB46) at d18 of embryogenesis reduced Enterococcus and Gram-negative bacterial colonization in the gut at hatch. An environmental challenge model was used to simulate microbial contamination in large-scale hatch cabinets in a laboratory setting. In all trials, gut homogenates obtained from chicks at hatch were plated to enumerate relevant bacterial populations. Performance was evaluated in trials two and four. The top …

The Mechanistic Target Of Rapamycin Mediates Clostridium Perfringens-Induced Chicken Necrotic Enteritis Attenuated By Secondary Bile Acid Deoxycholic Acid, Mohit Bansal, Tahrir Alenezi, Ying Fu, Janashrit Shrestha, Ayidh Almansour, Hong Wang, Anamika Gupta, Rohana Liyanage, Xiaolun Sun

Poultry Science Faculty Publications and Presentations

Clostridium perfringens is a prevalent gut bacterial pathogen in humans and animals. This study investigated the role of the mechanistic targets of rapamycin (mTOR) and deoxycholic acid (DCA) on C. perfringens intestinal infection. Chickens were sequentially infected with Eimeria maxima and received the mTOR inhibitor rapamycin and DCA. C. perfringens-induced necrotic enteritis (NE) was evaluated using body weight gain (BWG), histopathology, bile acids, pathogen colonization, cell infiltration and death, and gene expression. The significant difference of p < 0.05 was analyzed by one-way ANOVA and multiple comparisons. Notably, rapamycin strongly reduced the subclinical and clinical NE histopathologies. DCA and DCA combined with rapamycin alleviated clinical NE and BWG loss. Rapamycin, DCA, and DCA + rapamycin attenuated bile acid reduction in NE birds, and they also reduced immune cell infiltration into the intestinal lamina propria as well as immune cell migration in vitro. At molecular levels, DCA and DCA + rapamycin reduced proinflammatory IFN gamma, MMP9, IL23, and IL17 gene expression. Rapamycin, DCA, and DCA + rapamycin reduced NE-induced intestinal cell apoptosis. Together, these results suggest that mTOR signaling mediates C. perfringens-induced ileitis, and combining mTOR inhibition and DCA improves the intervention efficacy against NE ileitis and BWG loss.

Mitochondrial Dysfunction Is A Hallmark Of Woody Breast Myopathy In Broiler Chickens, Elizabeth S. Greene, Paula R. Chen, Carrie Walk, Mike Bedford, Sami Dridi

Poultry Science Faculty Publications and Presentations

The woody breast (WB) myopathy poses significant economic and welfare concerns to the poultry industry, however, there is no effective strategy to mitigate this pathology due to its unknown etiology. After showing previously that hypoxia is a key factor in WB progression, we used here various techniques demonstrating dysregulated mitochondria (morphology, biogenesis, tethering, function, and bioenergetics) in WB-affected muscles and in hypoxic myoblasts compared to healthy tissues and normoxic cells, respectively. The increased levels of calcium (Ca2+) in both WB-affected tissues and hypoxic myoblasts suggested that mitochondrial Ca2+ overload is likely a leading cause for mitochondrial dysfunction that merits further …

Characterization Of An Acetogenin-Carrying Nanosuspension And Its Effect On Bacteria Of Interest In The Poultry Industry, Brandon A. Lopez-Romero, Gabriela Aguilar-Hernandez, Billy Hargis, Maria De Lourdes Garcia-Magana, Ulises M. Lopez-Garcia, Rosa I. Ortiz-Basurto, Adalberto Zamudio-Ojeda, Juan D. Latorre, Efigenia Montalvo-Gonzalez

Poultry Science Faculty Publications and Presentations

This work aimed to develop a nanosuspension (NSps) as an acetogenin (ACGs) carrier, using soy lecithin (SL) and hydroxypropyl-beta-cyclodextrin (beta CD) named NSps-beta CDSL-ACGs. It was characterized by various spectroscopic techniques (DLS, FTIR, UV-vis diffuse reflectance). Moreover, the NSps morphology was observed by transmission electron microscopy (TEM). Also, the antibacterial activity of NSps-beta CDSL-ACGs was evaluated against strains of interest in the poultry sector. NSps-beta CDSL-ACGs presented nanometric size (207-239 nm), acceptable polydispersity index (PDI) values (0.13-0.17) and a high Z potential value (-47.17-50.36 mV), demonstrating high stability. The presence of ACGs in NSps-beta CDSL-ACGs was confirmed by FTIR analysis. …

Efficient Correction Of Dna Hetero–Duplexes Formed During Selex Procedure, Adil Sabr Al-Ogaili, Billy Hargis, Young Min Kwan

Poultry Science Faculty Publications and Presentations

Background:Aptamers, oligos-based (ssDNA or RNA) and peptide-based, are now emerging as the first-choice biomolecules in various applications in medicine and biology. DNA aptamers selection process includes repeatable steps of DNA amplification and digestion. The amplification process of the enriched DNA aptamers includes using PCR. Objectiveof study:In this article, we sought to describe an efficient and robust method that corrects or permanently removes the PCR by–products during the cycles of DNA aptamers selection. Materialsand Methods:Our method is simple, efficient, and requires no enzymatic treatment. In this method, we applied repetitive cycles of thermal treatment to the enriched aptamers final products during …

Efficacy Of Liver Free And Chitosan Against Eimeria Tenella In Chickens, Zhang Yu, Abdulaziz Alouffi, Ebtsam Al-Olayan, Gungor Cagdas Dincel, G. Tellez-Isaias, Inkar Castellanos-Huerta, Danielle Graham, Victor M. Petrone-Garcia, Beniamino T. Cenci-Goga, Luca Grispoldi, Luis Madeiro De Carvalho, Saeed El-Ashram

Poultry Science Faculty Publications and Presentations

Eimeria spp. are the pathogen that causes coccidiosis, a significant disease that affects intensively reared livestock, especially poultry. Anticoccidial feed additives, chemicals, and ionophores have routinely been employed to reduce Eimeria infections in broiler production. Therefore, the shift to antibiotic-free and organic farming necessitates novel coccidiosis preventive strategies. The present study evaluated the effects of potential feed additives, liver free and chitosan, against Eimeria tenella infection in White Leghorn broiler female chickens. One hundred sixty-five 1-day-old White Leghorn broiler female chicks were divided into 11 groups (15 female chicks per group), including the positive control group (G1), the negative control …

A Sublethal Concentration Of Chlorine Induces Antibiotic Resistance In Salmonella Via Production Of Reactive Oxygen Species, Mohammed Aljuwayd, Israa Abdullah Malli, Steven C. Ricke, Young Min Kwon

Poultry Science Faculty Publications and Presentations

Studies have shown that the production of reactive oxygen species (ROS) is triggered by bactericidal antibiotics, which contributes significantly to the killing of bacterial cells and increasing mutations in surviving cells. In this study, we hypothesized that exposure of Salmonella to sublethal concentrations of hypochlorite (NaOCl), a commonly used sanitizer in household and food industries increases mutation rates, leading to the development of antibiotic resistance. We found that a sublethal concentration (20 ppm) of NaOCl increased the mutation rates of S. typhimurium 14028s significantly (p < 0.05), which was prevented by the ROS scavenger thiourea, supporting that the increased mutation was due to NaOCl-triggered ROS production. We further found that the exposure of S. typhimurium 14028s to the same sublethal concentration of NaOCl increases resistance to kanamycin among the 3 antibiotics evaluated. The results of this study suggest that when NaOCl applied as a sanitizer fails to kill Salmonella due to diluted local concentrations or presence of organic materials, it can cause an adverse outcome of developing antibiotic resistance of the pathogen.

Efficacy Of Powdered Alfalfa Leaves To Ameliorate The Toxic Effects Of Aflatoxin B1 In Turkey Poults, M.J. Nava-Ramírez, Jesus A. Maguey-Gonzalez, S. Gomez-Rosales, J. O. Hernandez-Ramirez, Juan D. Latorre, Xiangwei Du, C. Lopez-Coello, Billy Hargis, G. Tellez-Isaias, A. Vasquez-Duran, A. Mendez-Albores

Poultry Science Faculty Publications and Presentations

Abstract

This experiment was conducted to determine the effect of an adsorbent material based on powdered alfalfa leaves added in the aflatoxin B₁ (AFB₁)-contaminated diet of turkey poults on production parameters, blood cell count, serum biochemistry, liver enzymes, and liver histology. For this purpose, three hundred and fifty female Nicholas-700 poults were randomly assigned into five treatments: (1) Control, AFB₁-free diet; (2) AF, diet contaminated with 250 ng AFB₁/g; (3) Alfalfa, AFB₁-free diet + 0.5% (w/w) adsorbent; (4) AF+alfalfa, diet contaminated with 250 ng AFB₁/g + 0.5% (w/w) adsorbent, …

Controlling Salmonella: Strategies For Feed, The Farm, And The Processing Plant, Tomi Obe, Timothy Boltz, Mike Kogut, Steven C. Ricke, Lasheda A. Brooks, Ken Macklin, Ashley Peterson

Poultry Science Faculty Publications and Presentations

Controlling Salmonella in poultry is an ongoing food safety measure and while significant progress has been made, there is a need to continue to evaluate different strategies that include understanding Salmonella-poultry interaction, Salmonella-microbiota interactions, Salmonella genetics and response to adverse conditions, and preharvest and postharvest parameters that enable persistence. The purpose of this symposium is to discuss different strategies to consider from feed milling to the farm to the processing environment. This Poultry Science Association symposium paper is divided into 5 different sections that covers 1) immunological aspects of Salmonella control, 2) application of Salmonella genetics for targeted …

Characterizing The Impact Of Enterococcus Cecorum Infection During Late Embryogenesis On Disease Progression, Cecal Microbiome Composition, And Early Performance In Broiler Chickens, Marcela Arango, Aaron Forga, Jing Liu, Latasha Gray, Randy Moore, Makenly E. Coles, Abdiel Atencio, Carolina Trujillo, Juan David Latorre, Guillermo Tellez-Isaias, Billy Hargis, Danielle Graham

Poultry Science Faculty Publications and Presentations

Enterococcus cecorum (EC) has been associated with septicemia and early mortality in broiler chickens. There is limited research investigating the pathogenicity of EC field strains obtained from affected birds. The purpose of this study was to evaluate the effect of in-ovo administration into the amnion with different EC field isolates at d 18 of embryogenesis (DOE18). In Exp 1, 7 EC field isolates alone or in combination (EC1–EC3, EC4–EC5, EC6, and EC7) were selected based on phenotypic characteristics and evaluated at different concentrations (1 × 10², 1 × 10⁴, and 1 × …

Potent Bile Acid Microbial Metabolites Modulate Clostridium Perfringens Virulence, Tahrir Jatli Alenezi, Ying Fu, Bilal Alrubaye, Thamer Alanazi, Ayidh Almansour, Hong Wang, Xiaolun Sun

Poultry Science Faculty Publications and Presentations

Clostridium perfringens is a versatile pathogen, inducing diseases in the skin, intestine (such as chicken necrotic enteritis (NE)), and other organs. The classical sign of NE is the foul smell gas in the ballooned small intestine. We hypothesized that deoxycholic acid (DCA) reduced NE by inhibiting C. perfringens virulence signaling pathways. To evaluate the hypothesis, C. perfringens strains CP1 and wild-type (WT) HN13 and its mutants were cultured with different bile acids, including DCA and isoallolithocholic acid (isoalloLCA). Growth, hydrogen sulfide (H₂S) production, and virulence gene expression were measured. Notably, isoalloLCA was more potent in reducing growth, H …

Isolation, Characterization, And Experimental Infection Of Streptococcus Gallolyticus Subspecies Pasteurianus From Commercial Turkeys With Acute Septicemia: A Pilot Study, L. S. Gray, J. D. Latorre, D. Hernandez-Patlan, B. Solis-Cruz, V. Petrone-Garcia, X. Hernandez-Velasco, K. M. Robbins, R. W. Moore, C. N. Vuong, A. Stein, L. Laverty, K. Martin, M. E. Coles, R. Señas-Cuesta, J. M. Diaz-Gomez, I. Loeza, I. Castellanos-Huerta, J. A. Maguey-Gonzalez, B. D. Graham, B. M. Hargis, G. Tellez-Isaias

Poultry Science Faculty Publications and Presentations

Streptococcus gallolyticus (SG) is a Gram-positive cocci found as commensal gut flora in animals and humans. SG has emerged as a cause of disease in young poults between 1 and 3 wk of age. SG is associated with septicemia resulting in acute mortality with no premonitory signs in turkeys. Three SG isolates were obtained from clinical field cases of acute septicemia of commercial turkeys and used in three independent experiments. In Experiment 1, embryos were inoculated 25 d of embryogenesis with varying concentrations of SG1, SG2, or SG3. In Experiment 2, day of hatch, poults were inoculated with varying concentrations …

Evaluation Of Clostridium Septicum Hemolytic Activity, Administration Route, And Dosage Volume Of A Clostridial Dermatitis (Cellulitis) Bacterin-Toxoid On Humoral Immune Response In Commercial Turkeys, Aaron Forga, Kabel Robbins, Andrew Smith, Makenly Coles, Guillermo Tellez-Isaias, Christine N. Vuong, Billy Hargis, Danielle Graham

Poultry Science Faculty Publications and Presentations

Clostridial cellulitis or dermatitis affects commercial turkey flocks, primarily as they approach market age. In the field, this disease has been effectively controlled with antibiotics, but alternatives to antibiotics are needed. Bacterin-toxoid vaccination programs have been shown to prevent clostridial diseases in other species, including humans. Results from previous field studies indicate that vaccination with an experimental whole-cell Clostridium septicum (CS) bacterin-toxoid oil emulsion vaccine reduced clostridial dermatitis-associated mortality and antibiotic usage for some commercial turkey flocks, but vaccination was not always efficacious. To improve vaccine efficacy, studies were conducted to optimize the antigenic component of the experimental …