Other Animal Sciences Commons^™

Evaluating Dietary Amino Acid Adequacy In Horses Using Isotopic Techniques, Sara L. Tanner

Theses and Dissertations--Animal and Food Sciences

Little is known about amino acid (AA) requirements in horses despite muscle mass accretion being of importance to an athletic species. Isotope methods for determining AA requirements and whole-body protein synthesis (WBPS) had not been previously used in growing horses. The first study herein was the first to apply isotope methods to determine WBPS in growing horses. In the study, weanling colts received two different levels of crude protein. Whole-body protein kinetics indicated that WBPS was greater when the weanlings were fed the diet with a greater crude protein content (P<0.05). The second study sought to determine a lysine requirement for yearling horses using the indicator AA oxidation (IAAO) method. Despite using six dietary levels of lysine; three above and three below the current recommendation, no breakpoint could be determined. Phenylalanine kinetics were not affected by lysine level (P>0.05), but plasma lysine increased linearly with lysine intake …

Evaluating The Effect Maturity On The Intake And Digestibility Of Switchgrass Hay Consumed By Beef Steers, David H. Davis

Theses and Dissertations--Plant and Soil Sciences

There has been increased interest in utilizing switchgrass (Panicum virgatum) as biomass. There are several challenges to developing this industry, and these have led to the potential use of switchgrass as hay for feeding beef cattle in Kentucky. The effect of increasing maturity on crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF) and nutritive values of switchgrass hay has been well documented, but few in vivo intake and digestibility trials have been conducted to assess this effect on animal performance when feeding beef cattle. Two in vivo intake and digestibility trials were conducted in 2011 …

Comparison Of Th1 Cytokines And T Cell Markers Gene Expressions Between Virulent And An Attenuated Eiav Vaccine Strain, Talia R. Henkle

Lewis Honors College Capstone Collection

The equine infectious anemia virus (EIAV) is closely related to HIV and has been used as a model to identify protective mechanisms against lentivirus infection. In horses, EIA infection progresses for about a year before infected horses manage to control virus replication. This naturally-gained protection is absolutely dependent on active immune responses as evidenced by the fact that immunosuppressive drugs can induce the recurrence of disease. As the resolution of initial viremia correlates with the appearance of virus specific cytotoxic T lymphocytes (CTL), we believe that cellular immune responses play a key role in controlling EIAV in the horse. In …

The Buzz On Bees, Rebecca Ruiz

Lewis Honors College Capstone Collection

Apis mellifera, more commonly known as the honeybee, plays a pivotal role in the ecosystem and in the survival of the planet. Many do not understand, nor realize, that the honeybee offers a wide array of products and services besides sweet honey. Honeybees have become endangered at a time when their presence is vital and evidence indicates that they can be the means to a more sustainable future for our planet. Particularly focused on in this research are the ideas of honeybees’ essential presence in medical advances, their participation in global economics, and their involvement in the development of …

Effects Of Endophyte Infected Fescue Alkaloid Ingestion On Energy Metabolism, Nitrogen Balance, In Situ Feed Degradation, And Ruminal Passage Rates, Anne F. Koontz

Theses and Dissertations--Animal and Food Sciences

The decrease in productivity caused by fescue toxicosis has been estimated to cost the United States livestock industry more than $1 billion per year due to reduced growth and diminished reproductive efficiency. This goal of the research presented in this dissertation is to enhance the knowledge base concerning the underlying physiological changes that occur during fescue toxicosis that lead to reduced intake and weight gain in cattle.

As one of the factors associated with fescue toxicosis is a reduction in feed intake, achieving a consistent and adequate intake of toxins can be a complication. Results from experiment 1demonstrate that ruminal …

Evaluation Of The Effects Of Vitamin K On Growth Performance And Bone Health In Swine, James S. Monegue

Theses and Dissertations--Animal and Food Sciences

The role of vitamin K in the blood clotting cascade has been well documented. Vitamin K has recently been implicated in improving bone health. The current studies were conducted to determine the effects of vitamin K in diets with and without mycotoxin contaminated corn on growth performance, bone characteristics, and related blood metabolites in pigs from weaning to market. Menadione sodium bisulfite complex (MSBC, 33% vitamin K) was chosen as the source of supplemental vitamin K because it is the most common form fed to swine. Vitamin K was tested at 0, 0.5, and 2.0 ppm in a corn-soybean meal …

Effect Of Estradiol Supplementation On Blood Estradiol And Metabolite Levels, And Hepatic Protein Expression, In Growing, Mature, And Senescent Beef Cattle, Edwena D. Miles

Theses and Dissertations--Animal and Food Sciences

Estradiol (Compudose®, COM) implants are extensively used in beef cattle production systems to alter body composition and feed efficiency. Little information exists about the physiological mechanisms affected by COM treatment in growing, mature, and senescent female cattle. Moreover, no reports describe the level of blood estradiol resulting from COM treatment. The effect of COM on levels of plasma estradiol and blood metabolites and proteins, and relative content of glutamine synthetase (GS) and other amino acid nitrogen-metabolizing enzymes in liver tissue, was studied using three experimental models relevant to cow-calf production regimens: senescent cows (Trial 1), young mature (young) versus senescent …

Use Of Mtb-100tm, Provided Through A Mineral Mix, To Reduce Toxicity When Lactating Beef Cows Graze Endophyte-Infected Tall Fescue, Melanie E. Hoar

Theses and Dissertations--Animal and Food Sciences

Two experiments were conducted at the University of Kentucky, Eden Shale Farm, Owenton, KY to evaluate the use of MTB-100^TM (Alltech, Inc., Nicholasville, KY) to alleviate the symptoms of fescue toxicity when lactating Angus x Beefmaster cows and their calves grazed endophyte-infected KY-31 tall fescue. Experiment 1 provided a carbohydrate based toxin adsorbent, MTB-100^TM, ad libitum in a commercial mineral supplement to project a daily consumption rate of 0, 20 or 40 g of MTB-100^TM per cow. Cows were weighed, assigned a body condition score (BCS) and hair coat score (HC), rectal temperatures were recorded and …

Evidence For The Maturation Of Cellular Immune Responses In Equine Infectious Anemia Virus-Infected Ponies, Chong Liu

Theses and Dissertations--Veterinary Science

Equine infectious anemia virus (EIAV) has been used as a model to investigate protective mechanisms against lentiviruses. Unlike other lentiviruses, EIAV replication can be eventually controlled in most infected horses leading to an inapparent carrier state free of overt clinical signs which can last for many years. Maintenance of this carrier state is absolutely dependent on active immune responses as evidenced by the fact that immunosuppressive drugs can induce the recurrence of disease. However, the immune mechanisms that are responsible for this control of infection are not yet identified. As the resolution of the initial infection is correlated with the …

Equine Serum Antibody Responses To Streptococcus Equi And Streptococcus Zooepidemicus, Rafaela De Negri

Theses and Dissertations--Veterinary Science

Streptococcus zooepidemicus (Sz) and Streptococcus equi (Se) share 98% DNA sequence homology, but display different pathogenic properties. Infection by one organism does not cross-protect against the other. To better understand pathogenic differences between these organisms and gain information about which proteins are expressed in horses infected experimentally with Se, intrauterine Sz or naturally with respiratory Sz we compared antibody specificities of convalescent sera using ELISA. These comparisons were based on sets of 8 and 14 immunoreactive recombinant proteins of Se strain CF32 and Sz strain NC78, respectively. Sera from donkeys that were previously naturally affected with strangles and later developed …

Development Of A New Allelic Discrimination Real-Time Pcr Assay For The Diagnosis Of Equine Herpesvirus-1 And Characterization Of The Virulence Determinants Of The Virus, Kathryn L. Smith

Theses and Dissertations--Veterinary Science

Equine herpesvirus-1 (EHV-1) can cause acute upper respiratory tract disease, abortion, neonatal death and neurological disease in horses. Rapid, accurate and timely diagnosis of EHV-1 infection in horses is important to curtail the spread of this pathogen. It has been reported that the neuropathogenic phenotype of EHV-1 can result from a single non-synonymous nucleotide substitution at position 2254 (A→G₂₂₅₄) in open reading frame 30 (ORF30). This was the basis for the development of an allelic discrimination, real-time PCR assay to distinguish between potential neuropathogenic and non-neuropathogenic EHV-1 strains. However, PCR analysis of a panel of EHV-1 abortion isolates …

Detection Of Antibodies Against Parascaris Equorum Excretory-Secretory Antigens, Steffanie V. Burk

Theses and Dissertations--Animal and Food Sciences

Parascaris equorum is a nematode parasite that infects young horses, sometimes causing unthriftiness, respiratory signs, or intestinal impaction in severe cases. Infection can be diagnosed by detection of eggs in feces, but this is only possible after the worms are fully mature. The goal of this study was to develop an antibody-based test for prepatent diagnosis of P. equorum infection. To produce western blot (WB) antigen, P. equorum larvae were cultured for collection of excretory-secretory antigens (ESA). Sera from 18 pregnant broodmares, their subsequent foals, and a group of 12 older mares and geldings were analyzed. In order to check …

Phosphorus Digestibility And Phytate Degradation In Long Yearlings And Mature Horses, Ashley Fowler

Theses and Dissertations--Animal and Food Sciences

Much of the phosphorus (P) in grain-based concentrates fed to growing horses is in the form of phytate-P. Little is known about the ability of young horses to degrade phytate-P or whether age affects mineral digestion in horses. The objective of this study was to examine the effect of age on P, calcium (Ca), and magnesium (Mg) digestibility and phytate-P degradation. Four long yearling geldings and 4 mature geldings were fed a diet of alfalfa cubes, timothy cubes and a pelleted concentrate. The diet contained 0.28% total P and 17.4% of that P was in the phytate form. There was …

The Effect Of Pituitary Pars Intermedia Dysfunction On Protein Metabolism And Insulin Sensitivity In Aged Horses, Laurel M. Mastro

Theses and Dissertations--Animal and Food Sciences

Equine pituitary pars intermedia dysfunction (PPID) typically occurs in horses older than 15 years of age and is characterized by hair coat abnormalities, muscle atrophy and decreased insulin sensitivity. The first objective of this research was to compare the rate of whole body protein metabolism and relative abundance of key factors in the signaling pathways associated with muscle protein synthesis and protein breakdown in response to feeding in Control and PPID horses. No differences (P > 0.05) were seen between the PPID and Control groups in whole-body protein metabolism or post-prandial activation of the muscle signaling pathways regulating skeletal muscle protein …

Influence Of Dietary Selenium Supplementation Form On Hepatic Transcriptome Profiles Of Maturing Beef Heifers, Zhi Zhang

Theses and Dissertations--Animal and Food Sciences

Our objective was to know how the hepatic transcriptome expression of growing beef (Angus-cross) heifers (0.5 kg gain/day) was affected by the feeding of different sources of dietary (3 mg/day) Se supplements: inorganic Se (ISe, sodium selenite), organic (OSe, Sel-Plex®), or a blend (1.5 mg:1.5 mg) of ISe:OSe (Mix), compared to the adequate but non-Se supplemented “Control”. The biopsied hepatic tissues of these four groups heifers collected at day 168 (when liver Se assimilation had stabilized) after supplements of Se, was subjected to the microarray analysis to assess Se treatment effects.

The results suggest that there were clear differences in …

The Effects Of Slow Release Urea On Nitrogen Metabolism In Cattle, Vaughn B. Holder

Theses and Dissertations--Animal and Food Sciences

The objective of this research was to investigate the effects of slow release urea on N metabolism in cattle. The ruminal behavior of Optigen®II and the effect of basal diet on the in situ degradability of urea and Optigen®II were evaluated. The effect of slow release urea and its interaction with degradable intake protein (DIP) level in the diet on N retention and excretion was evaluated utilizing 8 Holstein steers in a 4 x 4 Latin square experiment. In addition, the effect of slow release urea and DIP level on ruminal and systemic urea kinetics was evaluated using stable isotope …