Other Animal Sciences Commons^™

Managing Dietary Nonstructural Carbohydrates In Insulin Dysregulated Horses: Pasture, Long-Stem Forage, And Restrictive Grazing Strategies, Morgan J. Askins

Theses and Dissertations--Veterinary Science

Insulin dysregulation (ID) is a complex endocrine disorder, impacting a large percentage of the equine population. ID is the defining characteristic of equine metabolic syndrome (EMS). Within the various classifications of EMS horses, the commonality is ID and their higher risk of laminitis. To mitigate insulin response and laminitis risk in ID horses, current management focuses on restricting pasture exposure and feeding low nonstructural carbohydrate (NSC) rations. Minimal studies have evaluated how ID horses respond to pasture of various NSC, long-stem forages, and restrictive grazing strategies. Providing horse owners and managers with improved nutritional management recommendations for ID horses with …

Between The Rails: Experiential Education And Its Impact On Compliance And Consistency In Racetrack Surface Management, Heather Mackenzie

Theses and Dissertations--Community & Leadership Development

Recent regulatory requirements within the Thoroughbred racing industry have increased expectations for consistent collection and reporting of racetrack surface data. Despite clear standards, implementation in daily operations remains inconsistent, highlighting a gap between regulation, knowledge, and practice. This study examined the impact of an experiential education curriculum on racetrack personnel’s self-efficacy and reporting consistency.

Using a quantitative design, data were collected through pre- and post-surveys measuring self-efficacy and records of the number of days surface data were entered. Descriptive statistics, paired-samples t-tests, and effect size analyses were used. Results showed a statistically significant increase in self-efficacy (p < .001) with large effect sizes, indicating meaningful gains in confidence and comfortability. Reporting consistency did not show a statistically significant change (p = .885).

Overall, experiential education …

Gene Expression In Relation To Bone Fractures In Throughbred Racehorses, Anna M. Rauber-Ramos

Theses and Dissertations--Veterinary Science

Evidence that fractures in Thoroughbred racehorses are outcomes of chronic bone microdamage has led to extensive research into identifying horses at increased risk of fracture. This includes the use of biomarkers and messenger RNA (mRNA) as a measure of bone turnover. Previous research has found expression differences in horses with fractures compared to controls. Utilizing pre-race whole blood samples collected over an 18-month period from Thoroughbred racehorses in Southern California, RNA-sequencing was conducted on horses with bone fractures and race-matched controls to identify differences in gene expression. Focusing on third metacarpal (MC3) and proximal sesamoid bone (PSB) fractures, differentially expressed …

Impact Of Short-Distance Road Transportation On Horse Health, Erica Jacquay

Theses and Dissertations--Veterinary Science

Horses are regularly transported across the United States; however, the reasons for travel, trip lengths, and management practices are not well characterized. While long distance transportation has been associated with increased incidences of health-related problems, the impact of short distance transportation on horse health is less understood. Notably, aged horses ( > 15 years) have an altered immune response following short-distance transportation, but this has yet to be compared to young horses. It is also unknown whether transportation stress would alter endocrine responses of horses with metabolic disorders, such as insulin dysregulation (ID) or pituitary pars intermedia dysfunction (PPID).

The objectives …

Determining Population Structure In Kentucky Parascaris Univalens Specimens, Constance Finnerty

Theses and Dissertations--Veterinary Science

Parascaris univalens is a highly pathogenic nematode that affects the gastrointestinal system of young horses. When unaddressed, this parasite causes severe intestinal impactions and even death. Parascaris univalens is also found to have an emergence of anthelmintic resistances, causing a concern for horse owners. Despite its clinical significance, very little work has been done on Parascaris univalens to identify its population structure. This study aimed to determine the population structure of Parascaris univalens through Illumina next generation sequencing (NGS) on three hierarchical categorizations: within a horse, within an isolated herd, and within horses in the state of Kentucky. A total …

Genomic Studies Of The Equine Bloodworm, Strongylus Vulgaris, Nichol Ripley

Theses and Dissertations--Veterinary Science

Strongylus vulgaris is the most pathogenic nematode parasite of grazing horses worldwide. This gastrointestinal parasitic nematode is in the family Strongylidae under the subfamily Strongylinae, or Strongylins. While the epidemiology, complete life cycle, and high pathogenicity of the fourth and fifth larval stages of S. vulgaris are well established, we understand very little about its biology. This parasite is renowned for its unique life cycle and capacity to cause arterial damage, leading to significant health and economic impacts for the equine industry. It also is the most intensively documented Strongylin; however, S. vulgaris has yet to gain drug resistance as …

Evaluation Of Increasing Levels Of Mycotoxin-Containing Corn Fines On Diet Choice And Growth Performance Of Nursery Pigs, Duncan B. Paczosa

Theses and Dissertations--Animal and Food Sciences

Pigs fed mycotoxin contaminated diets at high concentrations will result in a detriment to health and performance. An initial study and a follow-up study were conducted to evaluate the effects of feeding increasing levels of mycotoxin-containing corn fines on growth performance, diet preference of pigs, and two possible mitigants.

The initial study was a 3 x 2 factorial containing three levels of fines (0, 10, and 20%, Diets 1-3, respectively) and either 0 ppm (Diets 1-3) or 40 ppm boron (Diets 4-6; Diets 4-6 were Diets 1-3, respectively, plus boron) as sodium tetraborate decahydrate as a possible mitigant. In this …

Molecular Genetic Studies Of Horses, Especially With Reference To Aggrecan And Dwarfism, John Edmund Eberth

Theses and Dissertations--Veterinary Science

This work consists of studies on dwarfism in Miniature horses and a study on breakdowns in Thoroughbreds in relation to gene aggrecan. A correction of the description and designation of D3 dwarf variant in aggrecan (ACAN) from the master’s thesis Chondrodysplasia-like dwarfism in the Miniature horse (2013). Commercial sequencing showed previous sequencing reads presented an artifact and not a single base deletion. Analysis showed a single base missense mutation in exon 8 identified as D3* was the actual cause. Multiple alleles of ACAN associated with chondrodysplastic dwarfism in Miniature horses by Eberth et al. (2018), corrected the …

Effects Of High-Speed Training On Messenger Rna Expression In Two-Year-Old Thoroughbred Racehorses, Mackenzie Grace Johnson

Theses and Dissertations--Veterinary Science

Accumulating high-speed exercise has been identified as a significant risk factor for catastrophic injuries in racing Thoroughbreds. Injuries, regardless of severity, are a main cause of withdrawal from the racing industry, raising animal welfare concerns and resulting in significant economic losses. While most of the current literature focuses on catastrophic injuries incurred during racing rather than training, the present study aims to help fill this gap as well as discuss the associated risk factors. The evaluation of messenger RNA (mRNA) expression changes provides an efficient and straightforward approach to identifying horses at risk for catastrophic injury. While alternative injury risk …

Use Of Molecular Genetics To Investigate Population Structure And Swayback In Horses, Navid Yousefimashouf

Theses and Dissertations--Veterinary Science

The present research incorporated molecular genetic methods to 1) investigate the genetic basis of Juvenile Onset Lordosis or Swayback in the American Saddlebred horses; and 2) conduct a population genetic study to compare the Persian Kurdish, Persian Arabian and American Thoroughbred horse populations.

Juvenile-onset lordosis, or swayback, is a condition in horses where the conformational topline back curvature drops significantly within the first two years of life. The trait has a higher prevalence in Saddlebreds (5%). Prior research on them quantified the trait using a Measurement of Back Contour (MBC), defining an MBC of >7.0 centimeters as swayback, and8.0) MBC …

Improving Indoor Arenas For The Equine Industry, Staci Mcgill

Theses and Dissertations--Biosystems and Agricultural Engineering

Equine indoor arenas are a unique infrastructure investment found at equine farms and facilities. They are semi-indoor structures for exercising horses, exhibiting skills during competitive events, and other equine related activities. These spaces do not always include mechanical ventilation or stirring fans and occupancy by horses and humans can be sporadic and inconsistent, which creates a challenging space for understanding and predicting airflow. Typically, indoor arenas have a sand-based footing over which the horse travels. The impact of the hooves can cause dust to become a concern within the facilities. Environmental concerns within these facilities (temperature, respirable dust, moisture, and …

Characterizing The Internal Porous Structure Of Equine Proximal Sesamoid Bones Subjected To Race Training Using Fast Fourier Transforms, Joseph Erik Davis

Theses and Dissertations--Biomedical Engineering

The equine racing industry is one of the main proponents in Kentucky’s economic infrastructure. In this industry there has come a need to investigate the nature of the proximal sesamoid bone (PSB). Breakdowns involving the PSBs are the leading cause in racehorse deaths in the industry, with still little known about what causes this bone to fracture. This study seeks to shed insight by investigating the internal structure of the PSB. Using microCT scanning, the internal porous structure was captured. From there, noticeable differences in the pores were noticed and quantified using fast Fourier transform (FFT) analysis.

The dominant peak …

The Microbiome Of The Equine Roundworm, Parascaris Spp., Jennifer Lynn Cain

Theses and Dissertations--Veterinary Science

Parasitic nematodes, including the large roundworms colloquially known as ascarids, affect the health and well-being of livestock animals worldwide. The equine ascarid, Parascaris spp., was the first ascarid parasite to develop wide-spread anthelmintic drug resistance, with other species slowly following suit. There are no new classes of anthelmintics currently in development, and a solution to the ever-increasing prevalence of resistance is desperately needed. The microbiome has been shown to be an important factor in the fitness and health of many organisms and changes to microbiome composition have been associated with a plethora of diseases. The microbiome is also important to …

Assessing Ear Pinna Repair In The Mrl/Mpj Mouse Strain, Fatemeh Safaee

Theses and Dissertations--Biology

The outcome of tissue repair varies across species. Vertebrates such as salamanders have the ability to not only heal scar-free but also completely regenerate lost appendages. In contrast, most mammals heal their wounds with fibrotic scarring. Understanding the key drivers of these divergent injury responses remains a major unanswered question in animal biology. Previous work with the Murphy’s Roth Large (MRL/MpJ) inbred mouse strain suggested they have the ability to rapidly close small (2mm) ear holes. While this ability was originally published as an example of regeneration, subsequent work by other groups suggested that it might represent something more akin …

Diet Of A Recently Reintroduced River Otter (Lontra Canadensis) Population In Taos County, New Mexico, Gabriela Alexandra Wolf-Gonzalez

Theses and Dissertations--Forestry and Natural Resources

North American river otters (Lontra canadensis), native to every U.S. state and Canada, experienced extensive population decreases and range reduction until the mid-20th century as a result of overexploitation and habitat loss during European colonization. The last known river otter in New Mexico was killed on the Gila River in 1953, although unverified reports continued thru 2008. After a nearly 60-year absence from New Mexico, 33 adult river otters were reintroduced to the Rio Pueblo de Taos in the northern part of the state between 2008-2010; however, they were not subsequently monitored or studied. I characterized diet of …

Trade-Offs Shape Carotenoid-Based Color Variation In Redheaded Pine Sawfly (Neodiprion Lecontei) Larvae, Maranda Gaines

Lewis Honors College Capstone Collection

Carotenoids serve various ecological roles in animals including coloration, immune responses, and vision. Carotenoid-derived coloration is greatly emphasized in the literature, particularly relating to mate choice and aposematic warning. However, the trade-offs between the color and non-color functions of carotenoids are not thoroughly explored. In the redheaded pine sawfly (Neodiprion lecontei), some larval populations have yellow pigmentation, using carotenoids derived from their diets for aposematic warning coloration. Other larval populations are white in color, having genetically lost the ability to produce the yellow pigment. Because carotenoids are essential to life functions in both the yellow and white populations, we aim …

Towards Determination Of The Threonine Requirement Of Yearling Horses Fed Varying Dietary Compositions Using The Indicator Amino Acid Oxidation Method, Kelsey M. Smith

Theses and Dissertations--Animal and Food Sciences

The amino acid requirements of growing horses are currently unknown, and studies suggest that threonine is a limiting amino acid in common horse diets. Thus, the objective of this study was to determine the threonine requirement of growing horses fed two different forage to concentrate ratios using the indicator amino acid oxidation method. The study consisted of a high concentrate phase (HC; 60% concentrate and 40% forage) and a high forage phase (HF; 25% concentrate and 75% forage). Within each phase, 6 female yearling Thoroughbred horses were randomly assigned each of 6 dietary treatments in a 6 x 6 Latin …

Effects Of Dietary Amino Acid Supplementation On Measures Of Whole-Body And Muscle Protein Metabolism In Aged Horses, Christine M. Latham

Theses and Dissertations--Animal and Food Sciences

Sarcopenia is a condition that is most common in aged animals, and is characterized by the loss of skeletal muscle mass and integrity, and can lead to physical disability and poor quality of life. Since skeletal muscle protein synthesis can be limited by the availability of amino acids, supplementation of limiting amino acids to ameliorate the progression of sarcopenia has become a topic of interest in companion animal research. Although there is some data to support the idea that amino acid supplementation improves maintenance of muscle mass in aged horses, the cellular mechanisms behind that improvement have yet to be …

Relationships Between Behavioral Measures And Productivity In Finishing Beef Cattle, Jasmyn Mimiko

Theses and Dissertations--Animal and Food Sciences

The relationship between measures of temperament, growth performance, and social hierarchy in finishing beef cattle were explored in two experiments. In experiment 1, high OCS (objective chute score) steers had periods of significantly higher ADG (P < 0.01), but OCS had no relationship with dominance ranking (P > 0.47). Conversely, slow exit velocity (EV) correlated with higher ranking (P ≤ 0.06), but EV had no relationship with performance (P > 0.37) in a competitive environment. Rank showed no relationship with performance (P > .58). In experiment 2, steers with fast EV had periods of decreased growth (P ≤ .06), intake (P ≤ .06), and gain:feed (G:F; P = 0.02). There were no interactions between …

Using The Indicator Amino Acid Oxidation Technique To Study Threonine Requirements In Horses Fed Different Feed Compositions, Chanhee Mok

Theses and Dissertations--Animal and Food Sciences

Threonine has been reported to be the second limiting amino acid in typical equine diets, but its actual requirement has not been determined in horses. The indicator amino acid oxidation (IAAO) method has been successfully used for evaluating amino acid metabolism and requirements in animals and humans. The objective of this research was to use the IAAO method to estimate threonine requirements in mature horses fed two different feed compositions. In the first study, 6 Thoroughbred mares (4 – 16 years old) received each of 6 levels of threonine intake in a high concentrate diet, in a randomly determined order. …

The Effect Of Dietary Nucleotides In Sow And Nursery Piglet Diets On Reproduction, Growth, And Immune Response, I-Fen Hung

Theses and Dissertations--Animal and Food Sciences

The objectives of these studies was to evaluate the effects of nucleotide (NT) supplementation of sow diets during late gestation and lactation and in nursery diets to elucidate the potential value of nucleotides for a variety of biological responses.

The studies conducted in the university research facilities showed that supplying nucleotides to sow diets during late gestation and throughout lactation had no effect on routinely measured reproductive responses or piglet growth postweaning. Nucleotides in the nursery diet had no effects on growth performance and intestinal morphology. However, the LPS-challenged pigs that consumed NT diets tended to lose less weight and …

Steroid-Dependent Regulation Of The Oviduct: A Cross-Species Transcriptomal Analysis, Katheryn L. Cerny

Theses and Dissertations--Animal and Food Sciences

Reproductive success depends on a functional oviduct for gamete storage, maturation, fertilization, and early-conceptus development. The ovarian-derived sex steroids estradiol and progesterone are known to affect functionality of the oviduct. Advances in microarray and NanoString technology allow for gene expression analysis to increase understanding of processes critical for fertility. Studies were conducted to investigate mechanisms regulating oviductal function in cattle and mice by using the Bovine Gene 1.0 ST array and the Mouse Gene 430-2.0 arrays (Affymetrix Inc., CA), respectively.

For the first study, oviducts were collected from heifers assigned to luteal or follicular phase groups. In the second study …

Animal And Pasture Responses To Grazing Management Of Chemically Suppressed Tall Fescue In Mixed Pastures, Jessica A. Williamson

Theses and Dissertations--Plant and Soil Sciences

Treatment of endophyte-infected tall fescue [Lolium arundinaceum (Schreb.) Darbysh] with the broad leaf herbicide Chaparral® can mitigate fescue toxicosis and enhance forage quality by suppressing seedhead emergence. Applying the herbicide to fescue pastures also reduces forage mass and promotes severe spot grazing when pastures are continuously grazed. A grazing experiment was conducted with steers (2013) and heifers (2014) to evaluate animal and plant responses in fescue-bluegrass (Poa pratensis L.) mixtures treated with Chaparral to determine the effects of grazing management on pasture carrying capacity, nutritive values, botanical composition, and animal performance. Continuous and rotational (i.e., four subdivisions to …

Gestational Form Of Supplemental Selenium (Se) Affects Steroidogenic Gene Expression In The Newborn Calf Testis, Stefani R. Garbacik

Theses and Dissertations--Animal and Food Sciences

Selenium (Se) is an important trace mineral in the diet of cattle. Our objective was to determine whether the form of supplemental Se fed to the dam would affect the expression of genes regulating steroidogenesis in the newborn testis. Twenty-four Angus-cross cows were assigned randomly (n=8) to individual ad libitum access of a mineral mix containing 35 ppm of Se supplied as sodium selenite (inorganic, ISe; Prince Se), Sel-Plex (organic, OSe; Sel-Plex, Alltech) or a 50/50 mix of ISe/OSe (MIX) 4 months prior to breeding and throughout gestation. All male calves were castrated within 2 days of birth and total …

Modeling Digestibility And Rate Of Passage In Horses, Tayler L. Hansen

Theses and Dissertations--Animal and Food Sciences

Horses consume large amounts of fiber in their diet, which may affect digestibility and digesta rate of passage. The objective of these studies was to assess the effects of diet composition on digestibility and rate of passage in horses using mathematical models. Using previously published data, forage dry matter digestibility (DMD) was related to forage neutral detergent fiber (NDF) concentrations and crude protein (CP) concentrations (DMD = 66.1178 – 0.3410*NDF + 0.6356*CP, DM basis; P < 0.001). In a second experiment, horses were fed diets formulated to have high fiber (HF, n= 3; NDF = 55.3%, CP = 11.8%) or low fiber (LF, n = 3; NDF = 40.5%, CP = 13.3%) concentrations. The LF treatment had greater DM, organic matter, and gross energy digestibilities (P < 0.05), whereas the HF treatment had greater NDF digestibility (P < 0.05). Mathematical models were useful to determine mean retention time (MRT) and there were no differences between model MRT and algebraic MRT, or treatment. Some compartmental parameters were different between treatments (P < 0.05), indicating that dietary fiber may alter some components of digesta passage in horses.

An Assessment Of Two Feed Additives To Improve Feed Utilization In Pigs, Amanda Shaw Thomas

Theses and Dissertations--Animal and Food Sciences

Three experiments were conducted to assess the efficacy of including selected feed additives in the diet of weaning and grow-finish pigs. Experiment 1 utilized 24 crossbred grow-finish pigs and measured the effect of added EHY on DM, N, and energy digestibility. There were no differences in DM, Energy, and N digestibility between diets 1 through 4. Experiment 2 utilized a total of 36 crossbred pigs [18 barrows, 18 gilts] in order to determine if preference would be shown when presented with naturally-contaminated corn. There were three dietary comparisons, Control vs Diet 2 (Comparison 1), Control vs Diet 4 (Comparison 2), …

The Initial Phases Of A Consistent Pricing System That Reflects The Online Sale Value Of A Horse, Curran A. Prettyman

Lewis Honors College Capstone Collection

Horses are one of the most uniquely priced commodities. This document provides a solution to an industry-wide weakness of inconsistent pricing and confusion. In the following report, an evaluation of the industry flaw is presented, an econometric approach is described in full, and a solution is proposed using insight gained from a regression analysis. This report uses an econometric approach to determine the impact of hunter jumper horse qualities on internet sale prices. Data is compiled from bigeq.com for seventy-eight horses in the states of Illinois, Indiana, Kentucky, Michigan, and Ohio. A linear regression analysis for twelve variables establishes that …

Modulation Of Vaccine-Induced Responses By Anthelmintic Treatment In Ponies, Emily Rubinson

Theses and Dissertations--Medical Sciences

Vaccines and anthelmintics induce an inflammatory response in equids. Since they are commonly given concurrently, it is practical to study any interaction between them. This study evaluated whether IVM and PYR would modulate the acute phase inflammatory response, the systemic gene expression of pro-inflammatory cytokines, and vaccine-specific titers induced by WNV, EHV, and KLH vaccines. Naturally-infected, yearling ponies were sorted by gender, then fecal epgs. They were randomly assigned to three treatment groups: IVM, PYR, and control. All ponies received vaccinations intramuscularly on days 0 and 29. Whole blood, serum, and plasma samples were collected 1, 3, and 14 days …

Effect Of Dietary Exposure To Ergot Alkaloids On Contractility Of Bovine Mesenteric Vasculature And Rumen Motility, Amanda M. Egert

Theses and Dissertations--Animal and Food Sciences

Endophyte-infected (E+) tall fescue grass has been associated with fescue toxicosis, a costly syndrome characterized by poor cattle performance and health resulting in significant production losses. The fungal endophyte produces ergot alkaloids, which help the grass thrive in poor conditions but are toxic to mammals. A number of symptoms of fescue toxicosis can be related to vasoconstriction of bovine core, peripheral, and foregut vasculature. The first part of this series of experiments demonstrated ergot alkaloids were also vasoactive in midgut vasculature, with the exception of lysergic acid. Additionally, prior dietary exposure to ergot alkaloids decreased the contractile response of mesenteric …

Effect Of Direct-Fed Microbials And Monensin On In Vitro Rumen Fermentation, Sheryl Wingard

Theses and Dissertations--Animal and Food Sciences

The impact of supplying a mixed culture of lactate producing bacteria on in-vitro rumen fermentation of forage- (Experiment 1) and concentrate- (Experiment 2) based diets in the presence and absence of monensin was explored. In experiment 1, interactions between DFM and MON were absent (P>0.10) for gas production and fermentative end products. Gas production and fermentative end products were unaffected by DFM alone (P>0.10). Monensin decreased (P<0.001) CH₄ and NH₃ production, rate and total gas production, as well as total VFA concentration and molar proportions of acetate and butyrate. However, MON increased (P<0.001) proportions of propionate, valerate, isobutyrate and isovalerate. Independently, DFM and MON increased (P<0.001) end point pH. In experiment 2, DFM x MON interactions or tendencies (P=0.07, P<0.01, P<0.01) were present, DFM effects were abated by MON, for rate, total gas production and total VFA concentrations. Acetate:propionate ratio was decreased (P<0.01) with MON and was unaffected by DFM. Ammonia-N concentration was increased (P<0.01) by DFM and unaffected (P=0.75) by MON. Both DFM and MON treatment increased (P<0.01) ruminal pH levels. These studies suggest the effects of DFM and MON of in-vitro fermentation are …