Other Animal Sciences Commons^™

In Vivo And In Vitro Digestibility Of A Complete Pelleted Feed In Horses, Cassandra Renee Sweeney

Master's Theses

ABSTRACT

COMPLETION OF AN IN VIVO DIGESTIBILITY TRIAL IN HORSES AND IN VITRO DIGESTIBILITY ASSAY DEVELOPMENT

Cassandra Renee Sweeney

In vivo analysis of equine feed digestibility has been the gold standard since the late 1800's, although it can be time consuming, costly, and labor intensive. In vitro digestibility analysis may be more economical and beneficial to both feed manufacturers and consumers. The availability of accurate in vivo data is crucial for critical evaluation and validation of any potential in vitro method (Coles et al., 2005). Ten adult American quarter horse geldings were used in the in vivo digestibility evaluation of …

Open Pulled Straw (Ops) Vitrification Of Mus Musculus Morula And Blastocyst Survival In Two Common Cryopreservation Medias, Gina Marie Cirimele

Animal Science

The objectives of this study were to: (1) determine an optimal cryoprotectant of mice embryos; and (2) determine whether morula or blastocyst stage is ideal for vitrification in both medias. One experiment was performed using two different medias for vitrification with open pulled straws (OPS) with morulae and blastocysts. In the first protocol, we called V, embryos were exposed to 10% ethylene glycol (EG) for 5 minutes, then 40% ethylene glycol and 0.6 M galactose for about 30 seconds, loaded into an OPS, and plunged into liquid nitrogen. In the second protocol, we called VG, embryos were exposed to 7.5% …

Design, Acnd Construction Of A Portable Artificial Insemination Chute, Chris Herrera

BioResource and Agricultural Engineering

This senior project report discusses the design, construction, and evaluation of a portable artificial insemination chute for the use of the Cal Poly Beef Department. The design was based on the suggested modifications from operators of several other chutes currently in use.

The main areas considered for the design were; Ergonomics, cattle safety, factors effecting maximum breeding conception rates, and Portability.

The breeding chute proved superior over traditional chutes in a variety of ways. Improvement occurred in the areas of consideration as follows, user operation was simplified, animal stress was decreased, and maximum breeding efficiency was obtained.

The Ma-10 Cell Line As A Model Of Insl3 Regulation And Leydig Cell Function, Mary E. Strong

Master's Theses

Leydig cells produce testosterone in response to luteinizing hormone (LH) via the cyclic adenosine monophosphate (cAMP)/protein kinase A pathway. Additionally, these cells are responsible for producing insulin-like peptide 3 (INSL3), a peptide hormone that is essential for testicular descent. The insl3 promoter in Leydig cells can be activated by cAMP through the transcription factor Nur77, which has also been shown to regulate the promoters of the steroidogenic enzymes, cyp17 and 3b-hsd. While the mechanism of LH action on testosterone production is well characterized, the effect of LH on insl3 abundance has yet to be shown directly. The MA-10 …

Caffeine Metabolism And Cytochrome P450 Enzyme Mrna Expression Levels Of Genetically Diverse Inbred Mouse Strains, Neal Addicott, Michael Malfatti, Gabriela G. Loots

STAR Program Research Presentations

Cytochrome P450 (CYP) enzymes activate or eliminate medications and other xenobiotics, and Caffeine has been used as an indicator of activity for some CYPs. The natural genetic variation in the human population for caffeine metabolizing CYPs does not yet have an established animal model for comparison, but the natural variation in inbred mouse strains presents an attractive starting point. The project gave four different strains of mice a 40mg/kg dose of caffeine and sampled blood and liver tissue after 30 minutes. Samples were analyzed using High Performance Liquid Chromatography (HPLC), reverse transcription PCR (rtPCR), and quantitative PCR (qPCR). The DBA/2J …

Rangeland Condition Assessment Of A Portion Of The Cal Poly Sheep Unit, Colin S. Mckim

Animal Science

This rangeland condition assessment was conducted as part of the Cal Poly Rangeland Condition Assessment Project. Five pastures comprising 31.7 acres at the Cal Poly sheep unit were assessed to collect baseline ecological information. Methods used were Line-point Intercept, Belt Transect, Residual Dry Matter, and the rangeland health indicators recommended by Pyke et al., 2002. Additionally, a preliminary species list was collected. The rangeland health assessment method found all pastures to have no to slight departure from the local reference area when evaluated for soil and site stability, hydrologic function, and biotic integrity.

Trial And Budget Analysis Of Two Synchronized Artificial Insemination Breeding Programs For Beef First Calf Heifers, John Nino

Agribusiness

This study was designed to compare the benefits and costs of two different synchronized artificial insemination programs on two groups of beef first calf heifers. A financial budget was developed that would include cost of synchronized insemination per animal and the added value of a bred heifer compared to an open one.

The two synchronized programs were called controlled internal drug release (CIDR) and Ovsynch. They used a specific timeline and drugs to complete the synchronization process. A single low birth weight high calving ease bull was used for both groups to enforce conformity between the groups. Costs of all …

Design, Planning, And Evaluation Of Upgraded Cattle Handling Facility, Lindsey Kaeli Tulloch

BioResource and Agricultural Engineering

This senior project discusses the design, planning, and evaluation of a livestock handling facility for Tulloch Ranches. The facility will be an upgrade to the facility currently used at the ranch in Pine Valley, California. The design parameters for the facility were narrowed down to the following: 1. Must reduce stress to animals during handling. 2. Must be cost effective to save labor and maintenance. 3. Must show improvement from the current set- up. The above parameters combine to form a system that enables the handlers of Tulloch Ranches to manage and work cattle in a more calm and humane …

Growth Hormone Alters Components Related To Differentiation, Metabolism And Milk Synthesis And Secretion In Mac-T Cells, Tasha Lynn Johnson

Master's Theses

The mammary alveolar cell-T (MAC-T) cell line is able to uniformly differentiate and secrete casein proteins in response to dexamethasone, insulin and prolactin and is extensively used to study bovine mammary epithelial cell function. Growth hormone (GH) has been shown to increase milk protein synthesis both in vivo and in mammary cell models, and induce cytoskeletal rearrangement in 3T3 fibroblast cell line and a Chinese hamster ovary (CHO) cell line. Few studies have focused on identifying the mechanisms involved in differentiated MAC-T cells’ response to GH. We tested the hypothesis that MAC-T cells would respond directly to GH and that …

Identifying Alternative Waste Conversion Technologies For Use At California Polytechnic State University San Luis Obispo, Matthew R. Bono

BioResource and Agricultural Engineering

This project was undertaken to identify and study alternative technologies for converting waste streams available on the Cal Poly San Luis Obispo campus into energy. As a large campus on the Central Coast of California Cal Poly not only produces lots of waste, but has limited options for its disposal. Furthermore, the campus is in the midst of an on-going green initiative, and more efficient use of its waste products would be a welcome development for the entire campus community. This report gathers information on waste streams originating at Cal Poly and information about waste to energy conversion technologies. The …

Student Sample Perceptions Of Organic Food Products And Buying Habits, Alycia M. Deus

Agricultural Education and Communication

The purposes of this senior project were to identify college students’ perceptions and knowledge of organic food. This study also focuses on the respondents’ purchasing and budgeting of organic food. This information is essential to inform farmers and ranchers because it serves as an indicator of future market’s demands.

A thirteen item questionnaire was distributed to sixty-five students attending California Polytechnic State University, San Luis Obispo, enrolled in either Agricultural Education and Communication courses or Agricultural Business courses. Both upper and lower division classes were surveyed. The questionnaires were distributed and collected from June 2, 2009 to June 4, 2009. …

Creating A Rabbitry For A High School Agricultural Program: A Guide For The Approval Process And Materials Required, Anne Marie Katuin

Agricultural Education and Communication

The proposal for a rabbitry to be added to the campus of Liberty High School in Golden Valley Unified School District in the designated agricultural area was proposed to the agricultural teacher and site administration for the high school. A floor plan was designed to fit the needs of a small rabbit breeding operation for the use of the LHS FFA students who wished to have a breeding or meat rabbit SAE.

Guidelines and plans were created in order to help an agricultural teacher or community member go through the previously mentioned approval process if they were to want to …

Sim-Plete Feeding Trial, Kelsey Johnson

Animal Science

A. An internship with ENI will offer an opportunity to work in the field with horse owners, feeds, marketing, and nutrition. I will be assisting in the placement of a product in the field and evaluating response. It is the first step in evaluating if this product is an appropriate and marketable product.

B. The responsibilities will be to place a new equine feed product with 10-15 horse owners (approximately 30-45 horses) and measure customer response and potential marketability of the product. Including:

1. Delivery of the product
2. Creation of Questionnaires (one will be given before the owners begin feeding the …