Meat Science Commons^™

A Life Cycle Analysis Of Land Use In Us Pork Production, Greg Thoma, Marty Matlock, Ben Putman, Jasmina Burek

Food Systems

The goal of this study was to analyze land use in the production of US pork using Life Cycle Assessment (LCA). LCA is a comprehensive methodology for quantitatively analyzing potential environmental impacts associated with complex systems. Identification of processes contributing to high environmental impacts often highlights opportunities for gains in efficiency, which can increase the profitability and sustainability of US pork. The environmental impact category analyzed in this assessment was land use. After reviewing existing information regarding land use in agriculture and livestock production, analysis for US pork production was performed at two scales: cradle-to-grave and cradle-to-farm gate. The cradle-to-grave …

Genetic Parameters For Docility, Weaning Weight, Yearling Weight, And Intramuscular Fat Percentage In Hereford Cattle, J. A. Torres-Vázquez, Matthew L. Spangler

Department of Animal Science: Faculty Publications

Cattle behavior, including measures of docility, is important to beef cattle producers not only from a human safety perspective but also due to potential correlations to economically relevant traits. Field data from the American Hereford Association was used to estimate genetic parameters for chute score (CS; n = 25,037), weaning weight (WW; n = 24,908), yearling weight (YW; n = 23,978), and intramuscular fat percentage (IMF; n = 12,566). Single-trait and bivariate animal models were used to estimate heritabilities and genetic correlations. All models included fixed effects of sex and contemporary group, defined as herd–year–season, and direct genetic and residual …

Production And Economic Efficiencies Of Intensified Cow-Calf Production Management Systems, Jason M. Warner

Department of Animal Science: Dissertations, Theses, and Student Research

The objective of four experiments conducted was to evaluate production and economic efficiencies of intensified cow-calf production management systems. The first experiment tested the effect of calf age at weaning on cow and calf performance and feed utilization at 2 locations. Body weight change from early to conventional weaning time was greater for early-weaned cows. Cow BCS and conception rates were not impacted by weaning. Calf BW at conventional weaning time was greater for conventionally-weaned calves than early-weaned calves at ARDC, but greater for early-weaned than conventionally-weaned calves at PHREC. Calf ADG per unit of total feed energy intake was …

Genomic Analysis Of Sow Reproductive Traits: Identification Of Selective Sweeps, Major Genes, And Genotype By Diet Interactions, Melanie D. Trenhaile

Department of Animal Science: Dissertations, Theses, and Student Research

Reproductive traits, such as litter size and reproductive longevity, are economically important. However, selection for these traits is difficult due to low heritability, polygenic nature, sex-limited expression, and expression late in life. Marker-assisted selection may provide an alternative to increase genetic progress.

Nebraska Index Line (NIL) has been selected for litter size related traits since 1981. It is one of the main contributing lines to the UNL reproductive longevity resource population (n > 1,500), which was genotyped for 60,000 SNPs, phenotyped for age at puberty (AP), lifetime number of parities (LTNP), litter size traits, and other reproductive traits, and fed either …

Failure Of A Heterologous Recombinant Sca5/Ompb Protein-Based Vaccine To Elicit Effective Protective Immunity Against Rickettsia Rickettsii Infections In C3h/Hen Mice, Sean P. Riley, Marissa M. Cardwell, Yvonne G. Chan, Ludovic Pruneau, Fabio Del Piero, Juan J. Martinez

Faculty Publications

Spotted fever group (SFG) rickettsial species are obligate intracellular tick-borne pathogens that are responsible for important human diseases. Previous reports have demonstrated the feasibility of using recombinant surface cell antigen Sca5/OmpB to elicit protective immunity against homologous challenges using murine models of Mediterranean spotted fever and Rocky Mountain spotted fever. In addition, the feasibility of generating cross-protective immunity against related rickettsial species has also been established, but the molecular basis for these phenomena was not explored. Here, we demonstrate that vaccination of C3H/HeN mice with a recombinant OmpB domain derived from Rickettsia conorii induced high titer humoral immune responses that …

Performance, Body Temperature And Blood Metabolites Of Feedlot Steers As Influenced By Environmental Conditions And Supplementation Of Zilpaterol Hydrochloride, Bradley M. Boyd

Department of Animal Science: Dissertations, Theses, and Student Research

Trial one was conducted at the United States Meat Animal Research Center (MARC) near clay center, NE during the summer of 2014. The objective of this trial was to measure the effects of supplementing zilpaterol hydrochloride (ZH) for the final 21 days of the finishing period, and shade, on performance, body temperature, respiration rate, and mobility of finishing beef steers. Feeding ZH increased hot carcass weight, dressing percent, longissimus muscle area, and reduce USDA yield grade. Shade did not affect steer performance and did not reduce body temperature. Zilpaterol hydrochloride increased respiration rate when compared to control cattle and had …

Effects Of Shade And Feeding Zilpaterol Hydrochloride To Finishing Steers On Performance, Carcass Quality, Heat Stress, Mobility, And Body Temperature, Bradley M. Boyd, Steven D. Shackelford, Kristin E. Hales, Tami M. Brown-Brandl, Meredith L. Bremer, Matthew L. Spangler, T. L. Wheeler, D. A. King, Galen Erickson

Department of Animal Science: Faculty Publications

Steers (n = 480; 22% with black hides and 78% with red hides) were used to study the effects of shade and feeding zilpaterol hydrochloride (ZH) on performance, carcass quality, heat stress, mobility, and body temperature (BT). A randomized block design with a 2 × 2 factorial treatment arrangement was used with 4 replicates per treatment. Factors included housing type (open or shaded pens) and the feeding of ZH (0 or 8.33 mg/kg DM) the last 21 d on feed with a 3-d withdrawal. Cattle were blocked by BW into a heavy or light block and randomly assigned to pen …

Evaluating Equations Estimating Change In Swine Feed Intake During Heat And Cold Stress, R. R. White, P. S. Miller, M. D. Hanigan

Department of Animal Science: Faculty Publications

The objectives of this study were to evaluate heat stress feed intake models for growing swine using a data set assembled from the literature and to develop a series of new equations modeling the influence of the thermal environment and interactions between the thermal environmental and other factors on feed intake. A literature survey was conducted to identify studies assessing intake responses to temperature. The resulting data set comprised 35 studies containing 120 comparisons to thermoneutral intake. Intake as a fraction of thermoneutral intake (FFI) was the primary response variable, where a value of 1 represented no change from thermoneutral …

Vegfa: Just One Of Multiple Mechanisms For Sex-Specific Vascular Development Within The Testis?, Kevin M. Sargent, Renee M. Sargent, Renata Spuri Gomes, Andrea S. Cupp

Department of Animal Science: Faculty Publications

Testis development from an indifferent gonad is a critical step in embryogenesis. A hallmark of testis differentiation is sex-specific vascularization which occurs as endothelial cells migrate from the adjacent mesonephros into the testis to surround Sertoli-germ cell aggregates and induce seminiferous cord formation. Many in vitro experiments have demonstrated that Vascular Endothelial Growth Factor A (VEGFA) is a critical regulator of this process. Both inhibitors to VEGFA signal transduction and excess VEGFA isoforms in testis organ cultures impaired vascular development and seminiferous cord formation. However, in vivo models using mice which selectively eliminated all VEGFA isoforms: in Sertoli and germ …

Economically Relevant Traits And Selection Indices, M. L. Spangler

Range Beef Cow Symposium

Sire selection should focus on that which is economical. This requires a true accounting of the traits that generate revenue or incur a cost within a specific beef cattle enterprise. Once the drivers of profit have been identified, sire selection should focus on the suite of traits that impact profitability. The use of bio-economic selection indices can dramatically reduce the complexity of multiple trait selection and aid in sire selection towards increased profitability. It is critical to use selection indices that match the intended production system. Using a terminal index in an enterprise that retains replacement heifers would not be …

Crossbreeding Strategies: Including Terminal Vs. Maternal Crosses, R. L. Weaber

Range Beef Cow Symposium

The dramatic changes and volatility cow-calf production system input costs and calf values have many producers wondering about the value of heterosis in today’s beef industry pricing structure. Many producers are seeking ways to improve cow-calf production efficiency and profitability. Profitability may be enhanced by increasing the volume of production (i.e. the pounds of calves you market) and/or the value of products you sell (improving quality). The reduction of production costs, and thus breakeven prices, can also improve profitability. Better yet, improving the input:output ratio should enhance profit. For commercial beef producers, the implementation of technologies and breeding systems that …

Using Partial Confinement Systems For Beef Cattle Production, Karla Jenkins

Range Beef Cow Symposium

Summary

• Energy density is the key to limit feeding
• Lactation increases energy needs considerably
• Consider early weaning options
• Confined calves must be able to reach water and feed
• Limit fed cows need ample bunk space and a consistent feeding routine
• Each producer needs to evaluate their resources and system options to see what might work best
• As prices change systems should be reevaluated

Cow Supplementation: Getting The Best Bang For Your Buck, Ken Olson

Range Beef Cow Symposium

How do we get the best bang for the buck?

• 2 aspects:

1. Biology: Use the right feeds to provide the right nutrients
2. Economics: price the best feeds to provide the right nutrients

High Altitude Disease, Pap, Feedlot Hypertension, And Respiratory Issues, J. M. Neary, F. B. Garry, T. N. Holt, G. M. Krafsur, P. S. Morley, R. D. Brown, K. R. Stenmark, R. Mark Enns, M. G. Thomas

Range Beef Cow Symposium

Summary

1. BRISKET DISEASE, or congestive heart failure, IS NOT UNIQUE TO HIGH ALTITUDE
2. Congestive heart failure is becoming INCREASINGLY PROBLEMATIC
3. TAKE A CLOSER LOOK – it may not be chronic pneumonia!

Economically Relevent Traits And Selection Indcies, Matt Spangler

Range Beef Cow Symposium

Summary

• Establish production goals
• Use economic indices that fit your desired breeding objectives
• Do not make sire selection more cumbersome than it needs to be
• Know your costs
• Select on PROFIT not just revenue
• Multiple trait selection is critical and could become more cumbersome
• Economic indexes help alleviate this
• Use index values that meet your breeding objective

High Altitude Disease, Pap, Feedlot Hypertension, And Respiratory Issues, J. M. Neary, F. B. Garry, T. N. Holt, G. M. Krafsur, P. S. Morley, R. D. Brown, K. R. Stenmark, R. Mark Enns, M. G. Thomas

Range Beef Cow Symposium

Right-heart failure (RHF) due to pulmonary hypertension, more commonly known as brisket disease or high altitude disease, is a complex disease that is becomingly increasing problematic for the cattle industry–regardless of altitude. The disease became known as high altitude disease because until the mid-1960s RHF was only reported at altitudes over 7,000 ft. Today, RHF is still problematic in high altitude cow-calf operations and is occurring with increasing incidence in feedlot cattle. The clinical signs are commonly mistaken for chronic pneumonia, which complicates disease diagnosis and reporting. Moreover, cattle treated for pneumonia have 2 to 3 times greater risk of …

Crossbreeding Strategies: Terminal And Maternal Crossing, Bob Weaber

Range Beef Cow Symposium

The Power of Crossbreeding

• Heterosis

• Superiority of a crossbred animal as compared to the average of its straightbred parents
• Especially maternal heterosis
• Breed Complementarity
• Selection of breeds for core traits that fill the other breed(s) shortcomings
• Maternal crossbreds-­‐appropriate cow size/lactation for environmental fitness
• Terminal crossbreds-­‐add value to calves in market place

Feedlot And Carcass Data: Making “Cents” And Making Decisions [Presentation], Julie Walker

Range Beef Cow Symposium

Summary

• Baseline of calves
• Management decision within the cow herd impacts performance within the feedlot and carcass characteristics
• Pre- and post- weaning performance is important in making management decisions

Feedlot And Carcass Data: Making “Cents” And Making Decisions, Julie Walker

Range Beef Cow Symposium

Sixty-two percent of calves are sold within 30 days of weaning (47% at weaning; McBride and Mathews, 2011). In the last decade, marketing of finished animals has shifted to selling on value-based systems. In 2012, nearly three times more cattle were sold in a value-based system compared to a traditional cash method. However, those two marketing methods accounted for nearly equal percentages of cattle sold as recently as 2006 (Mike Kasten, RBCS 2013). Therefore, carcass merit is a larger driver of value today compared to when the majority of cattle were sold on a live weight basis. How are feeder …

Antioxidant Effect Of Spent, Ground, And Lyophilized Brew From Roasted Coffee In Frozen Cooked Pork Patties, Katrina Maryse Malixi Jully, Criselda Toto, Lilian Were

Food Science Faculty Articles and Research

The ability of light and dark roasted coffee (1 g/kg) in varying application forms (spent ground [SCG], whole ground [WGC], or lyophilized brew [LBC]) to inhibit lipid and protein oxidation in cooked pork patties stored at −18 °C was monitored over 3 months. Malondialdehyde (MDA) for the negative control (NC) increased from 0.31 to 1.11 mg MDA/kg pork over 3 months, while pork with coffee or rosemary oleoresin had lower values at month 3 (0.054–0.40 mg MDA/kg pork). The NC had the highest values for hexanal, octanal, and nonanal (2.59, 0.10, and 0.13 mg/kg pork, respectively), while light and dark …

A Tail Of Two Phages: Genomic And Functional Analysis Of Listeria Monocytogenes Phages Vb_Lmos_188 And Vb_Lmos_293 Reveal The Receptor-Binding Proteins Involved In Host Specificity, Aidan Casey, Kieran Jordan, Horst Neve, Aidan Coffey, Olivia Mcauliffe

Department of Biological Sciences Publications

The physical characteristics of bacteriophages establish them as viable candidates for downstream development of pathogen detection assays and biocontrol measures. To utilize phages for such purposes, a detailed knowledge of their host interaction mechanisms is a prerequisite. There is currently a wealth of knowledge available concerning Gram-negative phage-host interaction, but little by comparison for Gram-positive phages and Listeria phages in particular. In this research, the lytic spectrum of two recently isolated Listeria monocytogenes phages (vB_LmoS_188 and vB_LmoS_293) was determined, and the genomic basis for their observed serotype 4b/4e host-specificity was investigated using comparative genomics. The late tail genes of these …

Prediction Of Plant Height In Arabidopsis Thaliana Using Dna Methylation Data, Yaodong Hu, Gota Morota, Guilherme J. M. Rosa, Daniel Gianola

Department of Animal Science: Faculty Publications

Prediction of complex traits using molecular genetic information is an active area in quantitative genetics research. In the postgenomic era, many types of -omic (e.g., transcriptomic, epigenomic, methylomic, and proteomic) data are becoming increasingly available. Therefore, evaluating the utility of this massive amount of information in prediction of complex traits is of interest. DNA methylation, the covalent change of a DNA molecule without affecting its underlying sequence, is one quantifiable form of epigenetic modification. We used methylation information for predicting plant height (PH) in Arabidopsis thaliana nonparametrically, using reproducing kernel Hilbert spaces (RKHS) regression. Also, we used different criteria for …

Evaluation Of Reduced Subsets Of Single Nucleotide Polymorphisms For The Prediction Of Age At Puberty In Sows, Katherine L. Lucot, Matthew L. Spangler, Melanie D. Trenhaile, Stephen D. Kachman, Daniel C. Ciobanu

Department of Animal Science: Faculty Publications

Genomic information could be used efficiently to improve traits that are expensive to measure, sex limited or expressed late in life. This study analyzed the phenotypic variation explained by major SNPs and windows for age at puberty in gilts, an indicator of reproductive longevity. A genome-wide association study using 56,424 SNPs explained 25.2% of the phenotypic variation in age at puberty in a training set (n = 820). All SNPs from the top 10% of 1-Mb windows explained 33.5% of the phenotypic variance compared to 47.1% explained by the most informative markers (n = 261). In an evaluation population, consisting …

Dietary Strategies For Mitigation Of Methane Production By Growing And Finishing Cattle, Anna C. Pesta

Department of Animal Science: Dissertations, Theses, and Student Research

Interest in reducing methane (CH₄) emissions by cattle has increased recently. Loss of feed energy as CH₄ represents both an environmental concern and an energetic loss to the animal. Manipulation of dietary composition has proven to be an effective mitigation strategy. Experiments were conducted to evaluate the effect of several dietary characteristics on CH₄ production in growing and finishing cattle, and to develop a novel, non-invasive method for quantifying emissions from animals in a production setting. Short-term gaseous emissions of CH₄ and carbon dioxide (CO₂) were measured and the CH₄:CO_{2 …}

Early Weaning Reduces Rangeland Herbage Disappearance, Patricia Johnson, Kenneth C. Olson, Roger N. Gates, Hubert H. Patterson, Mindy Hubert, Douglas Landbloom, Janna J. Kincheloe, Heather A. Richter, Allison V. Grove

Natural Resource Management Faculty Publications

Early weaning of beef calves reduces nutrient and forage demand in a cow–calf enterprise, potentially contributing to reduction in forage utilization on the pasture from which calves are removed by a nonlactating cow vs. a cow–calf pair. Research was conducted to evaluate weaning beef calves 90 days early (EW) vs. normal weaning (NW) on pasture herbage disappearance in mixed-grass prairie pastures in the northern Great Plains. Spring-calving cows (n = 48) were utilized in each study year (2003, 2004, and 2006) from the date of early weaning (August) until the date of normal weaning (November). Cow–calf pairs were randomly …

A Prototype On-Line Aotf Hyperspectral Image Acquisition System For Tenderness Assessment Of Beef Carcasses, Govindarajan Konda Naganathan, Kim Cluff, Ashok Samal, Chris Calkins, David D. Jones, Carol Lorenzen, Jeyamkondan Subbiah

Department of Food Science and Technology: Faculty Publications

A prototype on-line acousto-optic tunable filter (AOTF)-based hyperspectral image acquisition system (λ = 450– 900 nm) was developed for tenderness assessment of beef carcasses. Hyperspectral images of ribeye muscle on stationary hanging beef carcasses (n = 338) at 2-day postmortem were acquired in commercial beef slaughter or packing plants. After image acquisition, a strip steak was cut from each carcass, vacuum packaged, aged for 14 days, cooked, and slice shear force tenderness scores were collected by an independent lab. Beef hyperspectral images were mosaicked together and principal component (PC) analysis was conducted to reduce the spectral dimension. Six different textural …

Activity Of The Porcine Gonadotropin-Releasing Hormone Receptor Gene Promoter Is Partially Conferred By A Distal Gonadotrope Specific Element (Gse) Within An Upstream Enhancing Region, Two Proximal Gses And A Retinoid X Receptor Binding Site, Rebecca Cederberg, Jacqueline Smith, Emily A. Mcdonald, Chanho Lee, Amy R. Perkins, Brett R. White

Department of Animal Science: Faculty Publications

Background: Regulation of gonadotropin-releasing hormone (GnRH) receptor (GnRHR) numbers on gonadotropes within the anterior pituitary gland represents a critical point for control of reproductive function. Binding of GnRH to its receptor regulates follicle stimulating hormone (FSH) and luteinizing hormone (LH) release and levels of this G-protein coupled receptor on the surface of gonadotropes determines their sensitivity to GnRH pulses. While transcriptional regulation of this gene has been studied in mice, rats, humans and sheep, little is known about its regulation in the pig, an important agricultural species and human research model.

Methods: We isolated 5118 bp of 5′ flanking …

The Influence Of The Bovine Fecal Microbiota On The Shedding Of Shiga Toxin-Producing Escherichia Coli (Stec) By Beef Cattle, Nirosh D. Aluthge

Department of Food Science and Technology: Dissertations, Theses, and Student Research

During the past three decades, Shiga toxin-producing E.coli (STEC) have emerged as an important food safety concern. Although initially E. coli O157 was the main focus, recent outbreaks and resulting investigations have shown that certain non-O157 STEC are as much a threat to food safety as their O157 counterparts. To the beef industry, STEC have been of particular concern due to the frequent association of beef and beef products as vehicles of STEC infection. As a result, along with E. coli O157, six non-O157 STEC serogroups (known as the ‘big six’) are now regulated as adulterants in certain raw beef …

Effects Of Feeding Distillers Grains Throughout Different Phases Of Production On Shelf Life Of Ground Beef, Brandy D. Cleveland

Department of Animal Science: Dissertations, Theses, and Student Research

These studies analyzed the effects of feeding distillers grains on the shelf life of cooked and raw ground beef, and how the addition of postmortem or dietary antioxidants impact shelf life.

For study one, cattle were assigned to backgrounding diets containing low or high concentrations of wet distillers grains (WDGS) and either corn gluten feed or modified wet distillers grains (MDGS) during finishing. For study two, cattle were fed one of five finishing diets; corn, wet distillers grains (WDGS), WDGS + vitamin E, WDGS + Ethoxyquin/TBHQ, or WDGS + vitamin E + Ethoxyquin/TBHQ.

Shoulder clods from each dietary treatment were …

Impact Of Wet Distillers Grains Plus Solubles And Antioxidants On A Basic Mechanism Of Beef Tenderization, Michael D. Chao

Department of Animal Science: Dissertations, Theses, and Student Research

Feeding high levels of wet distillers grains plus solubles (WDGS) increases polyunsaturated fatty acid (PUFA) levels in beef. Perhaps, WDGS in feedlot diets increases PUFA concentration in the SR membrane, thereby altering membrane integrity, resulting in more rapid calcium leakage and improved tenderness. Feeding antioxidants may mitigate such effects. In the first study, effects of feeding 50 % WDGS on SR membrane composition, free calcium concentration and tenderness were studied. The SR membrane from steers fed WDGS were more tender, had higher free calcium concentration, had more PUFA, more phosphatidylcholine (PC), less phosphatidylethanolamine (PE) and less total phospholipids when compared …