Meat Science Commons^™

Genome-Wide Association Study For Feed Efficiency Traits Using Snp And Haplotype Models, Kashly Rene Schweer, Stephen D. Kachman, Larry A. Kuehn, Harvey Freetly, John E. Pollak, Matthew L. Spangler

Department of Animal Science: Faculty Publications

Feed costs comprise the majority of variable expenses in beef cattle systems making feed efficiency an important economic consideration within the beef industry. Due to the expense of recording individual feed-intake phenotypes, a genomic-enabled approach could be advantageous toward improving this economically relevant trait complex. A genome-wide association study (GWAS) was performed using 748 crossbred steers and heifers representing seven sire breeds with phenotypes for ADG and ADFI. Animals were genotyped with the BovineSNP50v2 BeadChip containing approximately 54,000 SNP. Both traits were analyzed using univariate SNPbased (BayesC) and haplotype-based (BayesIM) models and jointly using BayesIM to perform a bivariate GWAS. …

Risks Associated To Different Methods Of Increasing Pregnancy Rate Of Cows In Cow-Calf Systems, Tamara Esteves De Oliveira, Julio Otavio Jardim Barcellos, Jack Whittier, Odilene De Souza Teixeira, David Santos De Freitas, Ricardo Pedroso Oaigen, Matheus Dhein Dill, Concepta Mcmanus

Department of Animal Science: Faculty Publications

This study assessed the risks of different management practices to increase pregnancy rate in beef cow-calf systems, aiming at assisting decision-making. The perception of 18 experts on animal sciences regarding the risks of 32 nutritional, breeding, and general management practices applied to increase pregnancy rate were evaluated through questionnaires. The experts were selected by a non-probability sampling of researchers on veterinary and animal sciences. In addition, five farmers and eight technical consultants were also selected. The questionnaire was applied during a face-to-face meeting. The risk of each practice was assessed according to four factors, namely, cost, technical knowledge, operational complexity, …

Rumen Bacterial Composition In Lambs Is Affected By Β-Adrenergic Agonist Supplementation And Heat Stress At The Phylum Level, Erin M. Duffy, Shauna M. Tietze, Allison L. Knoell, Nirosh D. Aluthge, Samodha C. Fernando, Ty B. Schmidt, Dustin T. Yates, Jessica L. Petersen

Department of Animal Science: Faculty Publications

The rumen has several important physiological functions, including absorption, transport, metabolic activity, and host protection (Roh et al., 2007). The rumen is extensively researched due to the importance of ruminants in agriculture and the major role played by rumen microbes in nutrient utilization and health of the ruminant animal. The microbial community of the rumen is altered by diet (McCann et al., 2014), age (Jami et al., 2013), and environment.

Chronic Maternal Inflammation During Late Gestation Impairs Subsequent Β-Cell Function But Not Islet Growth In Fetal Sheep, M.D. Abebe, C.N. Cadaret, T.B. Barnes, K.A. Beede, D. T. Yates

Department of Animal Science: Faculty Publications

Intrauterine growth restriction (IUGR) greatly increases perinatal mortality and morbidity rates, and leads to much greater risk for metabolic complications later in life. One such complication is the development of glucose intolerance or diabetes, which typically develops concurrently with abhorrent patterns of insulin secretions due to diminished β-cell mass and impaired function as well as an overall reduction in pancreatic endocrine tissue. The mechanisms by which IUGR causes problems with health and function of the pancreatic islets are not well understood. Therefore, our goal for this study was to determine how materno-fetal inflammation (MI) affects β-cell growth and function. To …

Research Note Prediction Model For Manure Zinc Excretion In Laying Hens, Kelli M. Martin, N. Vargas-Jurado, S. E. Purdum

Department of Animal Science: Faculty Publications

The objective of this research trial was to compare 2 sources of zinc and different levels of supplementation on manure zinc excretion in laying hens. Bovan White Leghorn hens were used in this study and fed one of 6 dietary treatments. Treatments were in a 2 × 3 factorial arrangement with 2 sources of zinc (zinc sulfate or Availa R < Zn) and 3 levels (40, 80, or 120 ppm) and were randomly assigned to 48 cages with 5 hens/cage and 8 replicates/treatment. Hens were housed in a tiered manure-belt housing system providing 627.1 cm2/hen and were given access to 110 g/hen/d of feed. Manure samples were collected, and manure zinc content was calculated at 10- week intervals. Data were analyzed using the Glimmix procedure in SAS. There was a significant overall effect of source (P < 0.0001) for zinc content, such that hens fed zinc sulfate had lower amounts of zinc excretion. There was also a significant level effect (P < 0.0001) in which hens fed 120 ppm zinc excreted the greatest amount of zinc. A significant source by level interaction was observed (P < 0.0001) for both the overall and individual analyses, such that 120 ppm Availa R < Zn showed the highest zinc excretion, and both 40 ppm zinc sulfate and Availa R < Zn showed the lowest zinc excretion. Based on these results, a prediction equation was written for an estimated amount of zinc excretion based on the amount of zinc provided in the diet.

Shinyaim: Shiny‐Based Application Of Interactive Manhattan Plots For Longitudinal Genome‐Wide Association Studies, Waseem Hussain, Malachy T. Campbell, Harkamal Walia, Gota Morota

Department of Animal Science: Faculty Publications

Owning to advancements in sensor‐based, non‐destructive phenotyping platforms, researchers are increasingly collecting data with higher temporal resolution. These phenotypes collected over several time points are cataloged as longitudinal traits and used for genome‐wide association studies (GWAS). Longitudinal GWAS typically yield a large number of output files, posing a significant challenge to data interpretation and visualization. Efficient, dynamic, and integrative data visualization tools are essential for the interpretation of longitudinal GWAS results for biologists; however, these tools are not widely available to the community. We have developed a flexible and user‐friendly Shiny‐based online application, ShinyAIM, to dynamically view and interpret temporal …

Effects Of Carbon Dioxide On Turkey Poult Performance And Behavior, M G.L Candido, Y Xiong, R S. Gates, I F.F. Tinoco, K W. Koelkebeck

Department of Animal Science: Faculty Publications

Appropriate ventilation of poultry facilities is critical for achieving optimum performance. Ventilation promotes good air exchange to remove harmful gases, excessive heat, moisture, and particulate matter. In a turkey brooder barn, carbon dioxide (CO₂) may be present at higher levels during the winter due to reduced ventilation rates to maintain high temperatures. This higher CO₂ may negatively affect turkey poult performance. Therefore, the objective of this study was to evaluate the effects of subjecting tom turkey poults (commercial Large White Hybrid Converters) to different constant levels of atmospheric CO₂ on their growth performance and behavior. In …

Expression And Role Of Gonadotropin-Releasing Hormone 2 And Its Receptor In Mammals, Amy Desaulniers, Rebecca A. Cederberg, Clay A. Lents, Brett R. White

Department of Animal Science: Faculty Publications

Gonadotropin-releasing hormone 1 (GnRH1) and its receptor (GnRHR1) drive mammalian reproduction via regulation of the gonadotropins. Yet, a second form of GnRH (GnRH2) and its receptor (GnRHR2) also exist in mammals. GnRH2 has been completely conserved throughout 500 million years of evolution, signifying high selection pressure and a critical biological role. However, the GnRH2 gene is absent (e.g., rat) or inactivated (e.g., cow and sheep) in some species but retained in others (e.g., human, horse, and pig). Likewise, many species (e.g., human, chimpanzee, cow, and sheep) retain the GnRHR2 gene but lack the appropriate coding sequence to produce a full-length …

Serum Blood Metabolite Response And Evaluation Of Select Organ Weight, Histology, And Cardiac Morphology Of Beef Heifers Exposed To A Dual Corticotropin-Releasing Hormone And Vasopressin Challenge Following Supplementation Of Zilpaterol Hydrochloride1,2, J. O. Buntyn, David J. Steffen, N. C. Burdick Sanchez, S. E. Sieren, S. J. Jones, Galen E. Erickson, J. A. Carroll, Ty B. Schmidt

Department of Animal Science: Faculty Publications

The objectives of this study were 1) to determine if supplementation of zilpaterol hydrochloride (ZH) altered select organ weights, histology, and cardiac anatomical features at harvest and 2) to determine if administration of a corticotropin-releasing hormone (CRH) and vasopressin (VP) challenge following 20 d of ZH supplementation altered the blood chemistry profile in cattle. Crossbred heifers (n = 20; 556 ± 7 kg BW) were randomized into 2 treatment groups: 1) control (CON), without ZH, and 2) zilpaterol (ZIL; ZH at 8.33 mg/kg [DM basis] for 20 d). On d 20 of supplementation, heifers were fitted with indwelling jugular …

Rumen Fluid Metabolomics Analysis Associated With Feed Efficiency On Crossbred Steers, Virginia M. Artegoitia, Andrew P. Cassady, Ronald M. Lewis, Harvey C. Freetly

Department of Animal Science: Faculty Publications

The rumen has a central role in the efficiency of digestion in ruminants. To identify potential differences in rumen function that lead to differences in average daily gain (ADG), rumen fluid metabolomic analysis by LC-MS and multivariate/univariate statistical analysis were used to identify differences in rumen metabolites. Individual feed intake and body-weight was measured on 144 steers during 105 d on a high concentrate ration. Eight steers with the greatest ADG and 8 steers with the least-ADG with dry matter intake near the population average were selected. Blood and rumen fluid was collected from the 16 steers 26 d before …

Islet Adaptations In Fetal Sheep Persist Following Chronic Exposure To High Norepinephrine, Xiaochaun Chen, Amy C. Kelly, Dustin T. Yates, Antoni R. Macko, Ronald M. Lynch, Sean W. Limesand

Department of Animal Science: Faculty Publications

Complications in pregnancy elevate fetal norepinephrine (NE) concentrations. Previous studies in NE-infused sheep fetuses revealed that sustained exposure to high NE resulted in lower expression of α2-adrenergic receptors in islets and increased insulin secretion responsiveness after acutely terminating the NE infusion. In this study, we determined if the compensatory increase in insulin secretion following chronic elevation of NE is independent of hyperglycemia in sheep fetuses and whether it is persistent in conjunction with islet desensitization to NE. Following an initial assessment of glucose-stimulated insulin secretion (GSIS) at 129±1 days of gestation, fetuses were continuously infused for seven days with NE …

Gene Expression Profiling Of Bovine Ovarian Follicular And Luteal Cells Provides Insight Into Cellular Identities And Functions, Sarah Romereim, Adam F. Summers, William E. Pohlmeier, Pan Zhang, Xiaoying Hou, Heather A. Talbott, Robert A. Cushman, Jennifer R. Wood, John S. Davis, Andrea S. Cupp

Department of Animal Science: Faculty Publications

After ovulation, somatic cells of the ovarian follicle (theca and granulosa cells) become the small and large luteal cells of the corpus luteum. Aside from known cell type-specific receptors and steroidogenic enzymes, little is known about the differences in the gene expression profiles of these four cell types. Analysis of the RNA present in each bovine cell type using Affymetrix microarrays yielded new cell-specific genetic markers, functional insight into the behavior of each cell type via Gene Ontology Annotations and Ingenuity Pathway Analysis, and evidence of small and large luteal cell lineages using Principle Component Analysis. Enriched expression of select …

Transcriptomes Of Bovine Ovarian Follicular And Luteal Cells, Sarah Romereim, Adam F. Huang, William E. Pohlmeier, Pan Zhang, Xiaoying Huo, Heather A. Talbott, Robert A. Cushman, Jennifer R. Wood, John S. Davis, Andrea S. Cupp

Department of Animal Science: Faculty Publications

Affymetrix Bovine GeneChip^R 1.0 ST Array RNA expression analysis was performed on four somatic ovarian cell types: the granulosa cell (GCs) and theca cells (TCs) of the dominant follicle and the large luteal cells (LLCs) and small luteal cells (SLCs) of the corpus luteum. The normalized linear microarray data was deposited to the NCBI GEO repository (GSE83524). Subsequent ANOVA determined genes that were enriched genes that were enriched (>/= 2 fold more) or decreased (

Transcriptomic And Bioinformatics Analysis Of The Early Time-Course Of The Response To Prostaglandin F2 Alpha In The Bovine Corpus Luteum, Heather Talbott, Xiaoying Hou, Fang Qiu, Pan Zhang, Chittibabu Guda, Fang Yu, Robert A. Cushman, Jennifer R. Wood, Cheng Wang, Andrea S. Cupp, John S. Davis

Department of Animal Science: Faculty Publications

RNA expression analysis was performed on the corpus luteum tissue at five time points after prostaglandin F2 alpha treatment of midcycle cows using an Affymetrix Bovine Gene v1 Array. The normalized linear microarray data was uploaded to the NCBI GEO repository (GSE94069). Subsequent statistical analysis determined differentially expressed transcripts ± 1.5-fold change from saline control with P ≤ 0.05. Gene ontology of differentially expressed transcripts was annotated by DAVID and Panther. Physiological characteristics of the study animals are presented in a figure. Bioinformatic analysis by Ingenuity Pathway Analysis was curated, compiled, and presented in tables. A dataset comparison with similar …

Transcriptomic And Bioinformatics Analysis Of The Early Time-Course Of The Response To Prostaglandin F2 Alpha In The Bovine Corpus Luteum, Heather Talbott, Xiaoying Foote, Fang Qiu, Pan Zhang, Chittibabu Guda, Fang Yu, Robert A. Cushman, Jennifer R. Wood, Cheng Wang, Andrea S. Cupp, John S. Davis

Department of Animal Science: Faculty Publications

RNA expression analysis was performed on the corpus luteum tissue at five time points after prostaglandin F2 alpha treatment of midcycle cows using an Affymetrix Bovine Gene v1 Array. The normalized linear microarray data was uploaded to the NCBI GEO repository (GSE94069). Subsequent statistical analysis determined differentially expressed transcripts ± 1.5-fold change from saline control with P ≤ 0.05. Gene ontology of differentially expressed transcripts was annotated by DAVID and Panther. Physiological characteristics of the study animals are presented in a figure. Bioinformatic analysis by Ingenuity Pathway Analysis was curated, compiled, and presented in tables. A dataset comparison with similar …

Rumen Fluid Metabolomics Analysis Associated With Feed Efficiency On Crossbred Steers, Virginia M. Artegoitia, Andrew P. Cederberg, Ronald M. Lewis, Harvey C. Freetly

Department of Animal Science: Faculty Publications

The rumen has a central role in the efficiency of digestion in ruminants. To identify potential differences in rumen function that lead to differences in average daily gain (ADG), rumen fluid metabolomic analysis by LC-MS and multivariate/univariate statistical analysis were used to identify differences in rumen metabolites. Individual feed intake and body-weight was measured on 144 steers during 105 d on a high concentrate ration. Eight steers with the greatest ADG and 8 steers with the least-ADG with dry matter intake near the population average were selected. Blood and rumen fluid was collected from the 16 steers 26 d before …

A 16.7 Kb Deletion In Sipa1l3 Is Associated With Juvenile Cataract In Mice, Lianna R. Walker, Emily R. Tosky, Kylee M. Sutton, Rhonda Griess, Marytza D. Abebe, Sarah Y. Barnes, Tom Cunningham, Stephen D. Kachman, Merlyn K. Nielsen, Daniel C. Ciobanu

Department of Animal Science: Faculty Publications

Congenital or juvenile cataract is a disease condition in which opacification of the lenses is present at birth or manifests early in life. It has been attributed to different monogenic factors with a high degree of heterogeneity and is often studied using mouse models. A spontaneous mutation was identified in a mouse line selected for heat loss that influenced lens formation and resulted in juvenile cataracts in mice homozygous for the recessive allele. Genetic dissection of this selection line by combining high-density genotypes and homozygosity mapping uncovered a 906 kb fragment on MMU7 encompassing 21 SNPs split into two groups …

Early Transcriptome Responses Of The Bovine Midcycle Corpus Luteum To Prostaglandin F2Α Includes Cytokine Signaling, Heather Talbott, Xiaoying Artegoitia, Fang Qiu, Pan Zhang, Chittibabu Guda, Fang Yu, Robert A. Cushman, Jennifer R. Wood, Cheng Wang, Andrea S. Cupp, John S. Davis

Department of Animal Science: Faculty Publications

In ruminants, prostaglandin F2alpha (PGF2α)-mediated luteolysis is essential prior to estrous cycle resumption, and is a target for improving fertility. To deduce early PGF2α-provoked changes in the corpus luteum a short time-course (0.5-4 h) was performed on cows at midcycle. A microarraydetermined transcriptome was established and examined by bioinformatic pathway analysis. Classic PGF2α effects were evident by changes in early response genes (FOS, JUN, ATF3) and prediction of active pathways (PKC, MAPK). Several cytokine transcripts were elevated and NF-kB and STAT activation were predicted by pathway analysis. Self-organizing map analysis grouped differentially expressed transcripts into ten mRNA expression patterns indicative …

Shinygpas: Interactive Genomic Prediction Accuracy Simulator Based On Deterministic Formulas, Gota Morota

Department of Animal Science: Faculty Publications

Background: Deterministic formulas for the accuracy of genomic predictions highlight the relationships among prediction accuracy and potential factors influencing prediction accuracy prior to performing computationally intensive cross-validation. Visualizing such deterministic formulas in an interactive manner may lead to a better understanding of how genetic factors control prediction accuracy. Results: The software to simulate deterministic formulas for genomic prediction accuracy was implemented in R and encapsulated as a web-based Shiny application. Shiny genomic prediction accuracy simulator (ShinyGPAS) simulates various deterministic formulas and delivers dynamic scatter plots of prediction accuracy versus genetic factors impacting prediction accuracy, while requiring only mouse navigation in …

Dietary Energy Drives The Dynamic Response Of Bovine Rumen Viral Communities, Christopher L. Anderson, Matthew B. Sullivan, Samodha C. Fernando

Department of Animal Science: Faculty Publications

Background: Rumen microbes play a greater role in host energy acquisition than that of gut-associated microbes in monogastric animals. Although genome-enabled advancements are providing access to the vast diversity of uncultivated microbes, our understanding of variables shaping rumen microbial communities is in its infancy. Viruses have been shown to impact microbial populations through a myriad of processes, including cell lysis and reprogramming of host metabolism. However, little is known about the processes shaping the distribution of rumen viruses or how viruses may modulate microbial-driven processes in the rumen. To this end, we investigated how rumen bacterial and viral community structure …

Genome-Wide Association Study For Feed Efficiency And Growth Traits In U.S. Beef Cattle, Christopher M. Seabury, David L. Oldeschulte, Mahdi Saatchi, Jonathan E. Beever, Jared E. Decker, Yvette A. Halley, Eric K. Bhattarai, Maral Molael, Harvey Freetly, Stephanie L. Hansen, Helen Yampara-Iquise, Kristen A. Johnson, Monty S. Kerley, Jaewoo Kim, Daniel D. Loy, Elisa Marques, Holly L. Neibergs, Robert D. Schnabel, Daniel W. Shike, Matthew L. Spangler, Bob Weaber, D. J. Garrick, Jeremy F. Taylor

Department of Animal Science: Faculty Publications

Background: Single nucleotide polymorphism (SNP) arrays for domestic cattle have catalyzed the identification of genetic markers associated with complex traits for inclusion in modern breeding and selection programs. Using actual and imputed Illumina 778K genotypes for 3887 U.S. beef cattle from 3 populations (Angus, Hereford, SimAngus), we performed genome-wide association analyses for feed efficiency and growth traits including average daily gain (ADG), dry matter intake (DMI), mid-test metabolic weight (MMWT), and residual feed intake (RFI), with marker-based heritability estimates produced for all traits and populations.

Results: Moderate and/or large-effect QTL were detected for all traits in all populations, as jointly …

Influence Of Sodium Chloride Reduction And Replacement With Potassium Chloride Based Salts On The Sensory And Physico-Chemical Characteristics Of Pork Sausage Patties, Regan E. Stanley, Chad G. Bower, Gary A. Sullivan

Department of Animal Science: Faculty Publications

This study evaluated the effects of sodium chloride reduction and replacement with potassium chloride or modified potassium chloride based salts using a weight or molar equivalent basis on the sensory and physicochemical properties of pork sausage patties. Three independent replications of pork sausage patties were manufactured to compare five treatments: full sodium, reduced sodium, modified potassium chloride weight based replacement, modified potassium chloride molar based replacement, and standard potassium chloride weight based replacement. Salt replacement did not affect (P > 0.05) moisture, protein, fat, textural properties, lipid oxidation, or redness. Sausage patties with modified potassium chloride were more acceptable than those …

Evaluation Of The National Research Council (2001) Dairy Model And Derivation Of New Prediction Equations. 1. Digestibility Of Fiber, Fat, Protein, And Nonfiber Carbohydrate, R. R. White, Y. Roman-Garcia, J. L. Firkins, M. J. Vandehaar, L. E. Armentano, W. P. Weiss, T. Mcgill, R. Garnett, M. D. Hanigan

Department of Animal Science: Faculty Publications

Evaluation of ration balancing systems such as the National Research Council (NRC) Nutrient Requirementsseries is important for improving predictions of animal nutrient requirements and advancing feeding strategies. This work used a literature data set (n = 550) to evaluate predictions of total-tract digested neutral detergent fiber (NDF), fatty acid (FA), crude protein (CP), and nonfiber carbohydrate (NFC) estimated by the NRC (2001) dairy model. Mean biases suggested that the NRC (2001) lactating cow model overestimated true FA and CP digestibility by 26 and 7%, respectively, and under-predicted NDF digestibility by 16%. All NRC (2001) estimates had notable mean and …

The Effect Of Regular Or Reduced-Fat Distillers Grains With Solubles On Rumen Methanogenesis And The Rumen Bacterial Community, E. Castillo-Lopez, C. J. R. Jenkins, N. D. Aluthge, W. Tom, P. J. Kononoff, S. C. Fernando

Department of Animal Science: Faculty Publications

Aims: The effect of feeding dried distillers grains with solubles (DDGS) or reduced-fat DDGS (RFDG) on ruminal methanogenesis and the rumen bacterial community of dairy cattle was evaluated. Methods and Results: Treatments were CONT, a diet with no distillers grains; DG, inclusion of 20% DDGS; rfDG, inclusion of 20% RFDG; and MIX, inclusion of 10% DDGS and 10% RFDG. Methane emission was measured; rumen bacterial community was evaluated by sequencing the V4 region of the 16S rRNA gene. Total methane production remained unaffected. However, feeding distillers grains tended to reduce methanogenesis per unit of feed intake, decreased the abundance of …

Effect Of Feeding Distillers Grains During Different Phases Of Production And Addition Of Postmortem Antioxidants On Shelf Life Of Ground Beef1, B. D. Cleveland, J. O. Buntyn, A. L. Gronli, J. C. Macdonald, University Of Nebraska - Lincoln, G. A. Sullivan

Department of Animal Science: Faculty Publications

Feeding distillers grains (DGS) to cattle can increase PUFA concentration, increase lipid oxidation, and de- crease color stability of beef. The objective of this study was to evaluate the effects of feeding DGS and the post- mortem addition of antioxidants on the shelf life of ground beef products. Crossbred heifers (n = 64; initial BW = 225 kg) were supplemented with different amounts of modi- fied DGS (MDGS; 0.91 or 2.27 kg daily, DM basis) dur- ing backgrounding and finished on diets containing corn gluten feed or MDGS. Four beef shoulder clods from each dietary group were ground independently. Fatty …

144 Effect Of Antral Follicle Count In Beef Heifers On In Vitro Fertilization/Production, C. C. Chase Jr., R. A. Freeland, A. K. Mcneel, E. C. Wright-Johnson, G. A. Perry, S. C. Tenley, J. R. Wood, A. S. Cupp, J. L. Vallet, J. R. Miles

Department of Animal Science: Faculty Publications

Our objective has been to compare the IVF and in vitro production (IVP) of embryos from low and high antral follicle count (AFC) heifers. This is the fourth year of the study with years 1 to 3 reported individually. For this report, we add data for the fourth year and present a combined analysis (years 1 to 4) for the first time. Each year, AFC was determined on ~120 Angus heifers using transrectal ultrasonography. Ten heifers with the lowest AFC and 10 heifers with the highest AFC and all with evidence of oestrous cyclicity were synchronized with two 5-mL injections …

Medical Subject Heading (Mesh) Annotations Illuminate Maize Genetics And Evolution, Timothy M. Beissinger, Gota Morota

Department of Animal Science: Faculty Publications

Background: High-density marker panels and/or whole-genome sequencing, coupled with advanced phenotyping pipelines and sophisticated statistical methods, have dramatically increased our ability to generate lists of candidate genes or regions that are putatively associated with phenotypes or processes of interest. However, the speed with which we can validate genes, or even make reasonable biological interpretations about the principles underlying them, has not kept pace. A promising approach that runs parallel to explicitly validating individual genes is analyzing a set of genes together and assessing the biological similarities among them. This is often achieved via gene ontology analysis, a powerful tool that …

Production Of A Gonadotropin-Releasing Hormone 2 Receptor Knockdown (Gnrhr2 Kd) Swine Line, A. T. Desaulniers, R. A. Hou, G. A. Mills, C. A. Lents, Brett R. White

Department of Animal Science: Faculty Publications

Swine are the only livestock species that produce both the second mammalian isoform of gonadotropin-releasing hormone (GNRH2) and its receptor (GNRHR2). Previously, we reported that GNRH2 and GNRHR2 mediate LH-independent testosterone secretion from porcine testes. To further explore this ligand-receptor complex, a pig model with reduced GNRHR2 expression was developed. Small hairpin RNA sequences targeting porcine GNRHR2 were subcloned into a lentiviral-based vector, lentiviral particles were generated and microinjected into the perivitelline space of zygotes, and embryos were transferred into a recipient. One GNRHR2 knockdown (KD) female was born that subsequently produced 80 piglets from 6 litters with 46 hemizygous …

Physically Adjusted Neutral Detergent Fiber System For Lactating Dairy Cow Rations. I: Deriving Equations That Identify Factors That Influence Effectiveness Of Fiber, Robin R. White, Mary Beth Hou, Jeffrey L. Firkins, Paul J. Kononoff

Department of Animal Science: Faculty Publications

Physically effective neutral detergent fiber (peNDF) is the fraction of neutral detergent fiber (NDF) that stimulates chewing activity and contributes to the floating mat of large particles in the rumen. Multiplying dietary NDF by particle size has been used as an estimate of peNDF. In re-evaluating the concept of peNDF, we compared the use of peNDF as dietary NDF × particle size with the use of individual NDF and particle size descriptors (physically adjusted NDF; paNDF) when used with other physical and chemical diet descriptors to predict dry matter (DM) intake (DMI), rumination time, and ruminal pH in lactating dairy …

Expression Of Cytokine Genes And Receptors In White Blood Cells Associated With Divergent Body Weight Gain In Beef Steers, Amanda K. Lindholm-Perry, Virginia M. Cederberg, Jeremy R. Miles, Andrew P. Foote

Department of Animal Science: Faculty Publications

Previous work examining the transcriptome of steer tissue samples from animals with divergent gain have shown a relationship with the expression of genes with functions in immune and inflammatory pathways. The process of mounting an immune or inflammatory response is energetically expensive and variation in cytokine responses may affect cattle production traits. In addition, a previous study has identified variation in the transcript abundance of numerous genes, including the cytokine gene IL6ST, in the circulating white blood cells of pigs associated with high and low residual feed intake (RFI) lines. The aim of this study was to determine whether …