Meat Science Commons^™

Proteomic Analyses Identify Differences Between Bovine Epididymal And Ejaculated Spermatozoa That Contribute To Longevity, Saulo Menegatti Zoca, Emmalee J. Northrop-Albrecht, Julie A. Walker, Robert A. Cushman, George A. Perry

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Sperm are stored for extended periods of time in the epididymis, but upon ejaculation motility is increased and lifespan is decreased. The objective of this study was to identify differences in proteins between epididymis and ejaculated samples that are associated with longevity. Ejaculated semen was collected from mature Angus bulls (n = 9); bulls were slaughtered and epididymal semen was collected. Epididymal and ejaculated semen were centrifuged to separate sperm and fluid. Fluids were removed and sperm pellets were resuspended in a high ionic solution and vortexed to remove loosely attached proteins. Sperm samples were centrifuged, and the supernatant was …

Untangling The Placentome Gene Network Of Beef Heifers In Early Gestation, Wellison J.S. Diniz, Lawrence P. Reynolds, Alison K. Ward, Pawel P. Borowicz, Kevin K. Sedivec, Kacie L. Mccarthy, Cierrah J. Kassetas, Friederike Baumgaertner, James D. Kirsch, Sheri T. Dorsam, Tammi L. Neville, J. Chris Forcherio, Ronald R. Scott, Joel S. Caton, Carl R. Dahlen

Department of Animal Science: Faculty Publications

The cotyledon and caruncle tissues provide a functional bridge between the fetus and the dam. However, the relationship between these tissues and the transcriptomic profile that underlies the tissue functions remains elusive. Herein we investigate the expression profile of cotyledon and caruncle from nulliparous beef heifers carrying female fetuses at day 83 of pregnancy to identify changes occurring across tissues that contribute to placental function and their tissue-specific roles. We identified 2654 differentially expressed genes [padj ≤ 0.05, abs(log2FC) ≥ 1], including nutrient transporters and paternally imprinted genes. We found key regulators of tissue function and differentiation, including FOXO4, GATA2, …

Genetic Parameters, Heterosis, And Breed Effects For Body Condition Score And Mature Cow Weight In Beef Cattle, André Mauric F. Ribeiro, Leticia P. Sanglard, Warren M. Snelling, R. Mark Thallman, Larry A. Kuehn, Matthew L. Spangler

Department of Animal Science: Faculty Publications

Understanding the genetic relationship between mature cow weight (MWT) and body condition score (BCS) is useful to implement selection programs focused on cow efficiency. The objectives of this study were to estimate genetic parameters, heterosis, and breed effects for MWT and BCS. In total, 25,035 and 24,522 overlapping records were available for MWT and BCS on 6,138 and 6,131 cows, respectively, from the Germplasm Evaluation program, a crossbred beef population at the U.S. Meat Animal Research Center. Pedigree was available for 48,013 individuals. Univariate animal models were used to estimate heritabilities for each trait by parity. …

Effects Of Increasing Dietary Zinc Sulfate Fed To Gestating Ewes: Ii. Milk Somatic Cell Count, Microbial Populations, And Fatty Acid Composition, C. M. Page, R. M. Knuth, T. W. Murphy, D. C. Rule, B. Bisha, J. B. Taylor, W. C. Stewart

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Objective: The objective of the research was to evaluate the effects of increasing dietary Zn sulfate concentration for primiparous gestating ewes on subsequent milk SCC, intramammary microbial identifications, and fatty acid composition.

Materials and Methods: Commercial white-face (WF; n = 27) and black-face (BF; n = 24) ewes (age ≈18 mo; BW = 87.48 ± 8.37 kg) were sorted into breed-type groups and within groups ranked by BW, and then, they were randomly divided into 3 dietary supplement treatment groups: CON (n = 13; 40 mg/kg Zn; ≈1 × NASEM recommendations), Zn500 (n = 21; 500 mg/kg Zn; …

Effects Of Harvest Season On Carcass Characteristics Of Lambs In The Intermountain West, J. R. Whaley, T. W. Murphy, C. L. Gifford, W. J. Means, J. P. Ritten, H. N. Mckibben, C. M. Page, W. C. Stewart

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Objective: The objectives of this study were to survey characteristics including hot carcass weight (HCW), 12th rib fat thickness (RFT), body-wall thickness (BWT), longissimus muscle area (LMA), USDA yield grade (USDA YG), percentage closely trimmed retail cuts (RC), and calculated yield grade (Calc YG) of lamb carcasses in the Intermountain West to determine the effects of season of slaughter and interrelationships among carcass characteristics.

Materials and Methods: Lamb carcass characteristics were evaluated in 2 commercial Intermountain West processing plants over one year (n = 10,027). Carcasses were evaluated by season: spring (December–April, n = 2,322) and summer (May–August, n …

Beef Quality Following Prolonged Aging After Supplementing High Levels Of Vitamin E, Nicolas J. Herrera, Joseph A. Sonderman, David M. Velazco, Chris Calkins

Nebraska Beef Cattle Reports

Increased postmortem aging of beef can accelerate discoloration, shortening retail display time, inducing oxidation of lipids and proteins, and generating negative flavor attributes. This study was conducted to evaluate supplementation of high levels (2,200 International Units/head/day for 100 d) of Vitamin E (α-tocopherol) when feeding cattle as a strategy to sustain meat color quality in beef strip loins after prolonged aging. Results showed significantly less discoloration in loins from animals fed high levels of Vitamin E across 3, 6, and 9 weeks of aging. In addition, loins from cattle fed high levels of Vitamin E exhibited significantly greater redness (a*) …

2022 Nebraska Beef Cattle Report

Nebraska Beef Cattle Reports

The purpose of beef cattle and beef product research at the University of Nebraska-Lincoln is to provide reference information that represents the various populations (cows, calves, heifers, feeders, carcasses, retail products, etc.) of beef production.

Dedicated in memory of Dr. Terry Klopfenstein (March 10, 1939-April 30, 2021), Professor Emeritus, Animal Science Department, University of Nebraska-Lincoln.

Cow-Calf Nutrition and Management

Impact of Cow Size on Economic Profitability in the Cow-Calf and Feedlot Production Systems

Comparison of Partially Confined and Traditional Cow-Calf Systems

Concepts in Stress Physiology

Inflammatory Modulators Improve Daily Gain of Heat-Stressed Wethers

Zilpaterol Supplementation Improved Indicators of Well-Being, but …

Fate Of Salmonella In Beef Steaks During Sous Vide Cooking, Heather B. Hunt, Samuel C. Watson, Byron D. Chaves, Gary Sullivan

Nebraska Beef Cattle Reports

Sous vide cooking meat products has become a popular in-home method of food preparation. Previous research from UNL Meat Science using generic E. coli demonstrated the potential risk of sous vide cooking at temperatures recommended by some popular press items. To increase the understanding of the safety concerns of cooking beef products to temperatures below USDA-Food Safety and Inspection Service (FSIS) guidance temperatures, the experiment was conducted with Salmonella. Steaks were internally inoculated with three serovars of Salmonella, and sous vide cooked to internal temperatures of 115, 125, and 130° F. At least a 5 log₁₀ reduction was achieved …

Effects Of Lime Amendment On Antibiotic Resistance In Beef Cattle Manure Of Open Feedlots, Noelle A. Mware, Galen E. Erickson, Amy Schmidt, Shannon L. Bartelt-Hunt, Xu Li

Nebraska Beef Cattle Reports

The objective of this study was to evaluate the effectiveness of lime amendment on the reduction of antimicrobial resistant bacteria and antimicrobial resistance genes in beef cattle manure in open feedlots. Hydrated lime was uniformly applied to the surface of feedlot pen floor 1 day prior to cattle harvest at a rate of 0.36 lb/ft² and samples were collected over time. Collected samples were analyzed for change in pH and levels of antimicrobial resistant bacteria and antimicrobial resistance genes. Lime amendment elevated the pH of pen floor surface materials to pH > 12 for 4 hours and then pH > 11 …

Impact Of Spring Corn Residue Grazing On Soil Physical Properties And Crop Yield, Morgan T. Grabau, J. C. Macdonald, Zachary E. Carlson, M. E. Drewnoski

Nebraska Beef Cattle Reports

The effects of stocking density for spring corn residue grazing on soil physical properties and soybean yield were evaluated in an experiment with three treatments: no grazing, normal density, and high density. Normal density was stocked at 3 calves/acre and grazed 45 days starting in mid-February. High-density was stocked at 9 calves/acre and grazed 15 days starting in mid-March. Increased stocking density decreased residue cover and increased surface roughness. Bulk density and penetration resistance were increased for normal density compared to no graze, with no difference between grazed treatments, illustrating that grazing may cause minor compaction, regardless of stocking density. …

Evaluation Of Methane And Co2 Production In Growing And Finishing Cattle Raised In Conventional Or Partial Confinement-Based Herds, L. J. Mcphillips, Z. E. Carlson, Rick R. Stowell, J. C. Macdonald, Andrew E. Suyker, Galen E. Erickson

Nebraska Beef Cattle Reports

Methane emissions from growing and finishing calves compared either a spring calving, conventional cow system or a summer calving, partially-confined cow system. Calves weaned from the confinement-based production system were smaller at weaning and compensated with greater gain during the growing phase. More days on feed in the finishing phase were needed for the calves from the confinement system to reach same backfat thickness. Over the entire growing and finishing phases, calves from the confinement-based system produced more total CH₄ and CH₄ per lb. HCW. Production of methane and CO₂ per lb. of gain was lower in …

Impact Of Biochar Supplementation In Finishing Diets On Greenhouse Gas Emissions, J. L. Sperber, Braden C. Troyer, Mitchell M. Norman, Levi J. Mcphillips, Heather Nobert, Andrea K. Watson, Galen E. Erickson

Nebraska Beef Cattle Reports

A study was conducted to evaluate the impact of feeding biochar in a finishing diet on cattle performance, methane and carbon dioxide emissions, and carcass characteristics. Two treatments were evaluated, a high-concentrate control diet without biochar and a diet with biochar included at 1.0% of the diet dry matter, replacing high moisture corn in the diet. Pens of cattle were rotated through a two-sided emissions barn (2 pens evaluated simultaneously) to capture CH4 and CO2 production. There were no statistical differences in gas emissions for biochar supplemented steers compared to control. There was a 2.4% decrease in dry matter intake …

Impact Of A Natural Feed Additive Using Direct Fed Microbes On Finishing Beef Cattle Performance And Liver Abscess Rate, Kelton C. Adair, Alison C. Bartenslager, Zachary E. Carlson, Galen E. Erickson, Samodha C. Fernando, J. C. Macdonald

Nebraska Beef Cattle Reports

A feedlot study with individually fed steers (n=60) was conducted comparing a natural feed additive (Direct Fed Microbial) to no feed additive (control) on performance and liver abscess rate of finishing beef cattle. There were no significant differences between treatments on intake, gain, feed conversion, or hot carcass weight and carcass traits. Liver abscess occurrence and severity were similar for both treatments with 4/29 steers having abscessed livers in the control group and 3/29 steers in the group that received direct fed microbials. The direct fed microbial feed additive fed in this exploratory study did not significantly affect performance, liver …

Evaluation Of Processing Technique For High-Moisture And Dry Corn On Nutrient Digestion When Fed To Finishing Cattle, Caitlin A. Coulson, Mitch M. Norman, Nicole M. Woita, Hannah C. Wilson, Kylie M. Butterfield, Tyler J. Spore, Galen E. Erickson

Nebraska Beef Cattle Reports

A digestion trial was conducted to determine the effect of corn milling method when processing dry or high-moisture corn on nutrient digestion. Treatments were evaluated as a 2 × 2 factorial with the first factor as corn type [dry corn or high-moisture corn] and second factor as mill type [roller mill or hammer mill]. Feeding high-moisture corn decreased the amount of excreted dry matter and organic matter regardless of processing method, but there tended to be an interaction between corn type and milling method for digestibility. There was no difference between milling treatments fed as high-moisture corn, but hammer milled …

Impact Of Steam-Flaked Rye Fed In Combination With Steam-Flaked Corn On Performance And Carcass Characteristics Of Yearling Steers, Samantha K. Wagner, Braden T. Troyer, Levi J. Mcphillips, Rebecca S. Brattain, Galen E. Erickson

Nebraska Beef Cattle Reports

A finishing study evaluated the effect of feeding steam-flaked rye on performance and carcass characteristics of yearling steers. Treatments consisted of different ratios of steam-flaked rye to steam-flaked corn. The ratios were 0:100, 25:75, 50:50, and 100:0 rye:corn. Increasing inclusions of rye linearly decreased final body weight, dry matter intake, average daily gain, and hot carcass weight. As a result, feed conversion was poorer as rye replaced corn. Carcass characteristics reflected lower gain with linear decreases in marbling score, ribeye area, and backfat thickness. There was no difference in yield grade among treatments. Based on dietary energy calculated from performance, …

Evaluating Performance Of Cattle Fed Steam-Flaked Corn Based Finishing Diets Fed Increasing Inclusions Of Wet Or Modified Distillers Grains, Braden C. Troyer, Levi J. Mcphillips, Mitchell M. Norman, Galen E. Erickson

Nebraska Beef Cattle Reports

A finishing trial using 560 calf-fed steers was conducted to evaluate the effect of distillers type and inclusion on finishing cattle performance and carcass characteristics in steam-flaked corn based diets. Treatments were applied in a 2 × 3 + 1 factorial arrangement, with the first factor being distillers type (modified distillers grains plus solubles or wet distillers grains plus solubles) and the second factor being distillers grains inclusion of 10%, 20%, or 30%, as well as a control diet containing no distillers. There was an interaction between inclusion and type of distillers for feed conversion. A linear improvement in feed …

Effect Of Finishing Cattle With Blends Of High Moisture And Steam-Flaked Corn With And Without Distillers Grains, Braden C. Troyer, Zac C. Carlson, Levi J. Mcphillips, Mitchell M. Norman, Andrea K. Watson, J. C. Macdonald, Galen E. Erickson

Nebraska Beef Cattle Reports

The objective of this study was to determine the impacts of feeding different ratios of high-moisture corn and steam-flaked corn in diets with or without distillers grains and test for an associative effect between grain processing methods. Steers (n=120; BW=775 ± 15.6 lb) were assigned randomly to one of 8 diets. Diets tested included 100:0, 75:25, 50:50, 25:75, and 0:100 of high-moisture corn:steam-flaked corn in diets with 20% modified distillers grains or 100:0, 50:50, and 0:100 in diets without distillers grains. There was an interaction observed for average daily gain as gain improved in 50:50 blends of HMC:SFC in diets …

Characterizing Digestion Traits Of Novel Corn Bran Products, Jiehua Xiong, Chanon Suntara, Rebecca Sjostrand, Tyler Spore, Maggie Youngers, Rick Stock, Galen E. Erickson, J. C. Macdonald

Nebraska Beef Cattle Reports

A digestion study was conducted to evaluate novel corn bran products from Cargill Corn Milling on nutrient digestibility in beef steers. Three bran products (Bran A/B/C) were included at 70% of diet dry matter of TRT A, B and C, with an internal control (SFC control) diet which consisted of 70% SFC. The SFC control exhibited the greatest dry matter (DM), organic matter (OM) and starch digestibility, and the least neutral detergent fiber (NDF) digestibility. Among TRT A, B and C when bran products were included at 70%, there was no difference in DM or OM intake and digestibility. NDF …

Interaction Of Sweet Bran Inclusion And Corn Processing Method In Beef Finishing Diets On Digestibility, Rebecca L. Sjostrand, Kathlyn Hauxwell, Maggie Youngers, Rick Stock, J. C. Macdonald, Galen E. Erickson

Nebraska Beef Cattle Reports

Six ruminally and duodenally fistulated steers were utilized in a 6 × 6 Latin square design to evaluate the interaction of corn processing method and Sweet Bran inclusion in finishing diets on total tract digestibility and rumen fermentation characteristics. Treatments were designed as a 2 × 3 factorial, with one factor as corn processing method (steam-flaked corn or a blend of high-moisture and dry-rolled corn) and the second factor as Sweet Bran inclusion at 0, 20, or 40% of diet dry matter. Cattle fed steam-flaked corn diets had greater starch digestibility while cattle fed high-moisture/ dry-rolled corn diets had greater …

Evaluation Of Models To Predict Dry Matter Intake Of Forage Based Diets, Aksel Wiseman, Andrea K. Watson, Rick Stock, Terry Klopfenstein

Nebraska Beef Cattle Reports

Dry matter intake (DMI) data from growing cattle experiments at the Eastern Nebraska Research and Extension Center were summarized in order to evaluate the accuracy of model predicted DMI. Cattle were fed individually (n = 78) or in pens (n = 15) and predicted DMI using the Beef Cattle Nutrient Requirements Model (BCNRM, 2016) was compared to observed DMI. The model over predicted DMI when total digestible nutrients (TDN) was less than 64%, under predicted DMI when dietary TDN was greater than 64%, and had a low accuracy, explaining less than 22% of the variation in DMI. An equation to …

Interaction Of Urea With Frequency And Amount Of Distillers Grains Supplementation For Growing Steers, Haley F. Linder, Zachary E. Carlson, M. E. Drewnoski, J. C. Macdonald

Nebraska Beef Cattle Reports

A study was conducted to determine the interaction of urea with the frequency and amount of distillers grain supplementation for growing steers consuming forage. Steers were individually fed for 84 d and received ad libitum grass hay and 1 of 8 treatments. Treatment design was a 2 × 2 × 2 factorial. Supplement was fed either every day or 3x/ week, amount of supplement fed was 13.99 lb/week or 28.00 lb/week, and contained either no urea or 1.3% urea. Supplementation frequency and the inclusion of urea did not impact steer average daily gain. The amount of supplement did impacted steer …

Impact Of Urea On Corn Silage Growing Cattle Diets, Tyler J. Spore, Hannah C. Wilson, Nolan R. Meier, Galen E. Erickson, James C. Macdonald, Terry J. Klopfenstein, Andrea K. Watson

Nebraska Beef Cattle Reports

Two experiments were conducted to evaluate the effects of increasing urea in a corn silage growing cattle diet and ensiling time impact on rumen undegradable protein content of corn silage. Four treatments (urea inclusion at 0, 0.5, 1, and 1.5% of diet dry matter) were evaluated in diets containing 95% corn silage using 10 ruminally and duodenally cannulated heifers. Corn silage was sampled 5 times (every 32 days) to determine rumen undegradable protein content. Intake and total tract digestibility of dry matter, organic matter, and neutral detergent fiber all increased linearly with increasing inclusion of urea in the diet. Measured …

Growth Performance In Livestock With Stress-Induced Low Birthweight Is Recovered By Clenbuterol Administration, Rachel L. Gibbs, Rebecca M. Swanson, Joslyn K. Beard, Ty B. Schmidt, Jessica Lynn Petersen, Dustin T. Yates

Nebraska Beef Cattle Reports

Stress in pregnant livestock causes pathological low birthweight of the offspring and diminishes their muscle growth capacity. Using sheep as a model for beef cattle, this study determined that the limited growth capacity of offspring with stress-induced low birthweight could be recovered by postnatal treatments. Muscle in these animals has less growth-promoting β2 adrenergic activity and thus the injectable β2 adrenergic stimulant clenbuterol was used as a daily treatment. Lambs with stress-induced low birthweight continued to exhibit impaired muscle growth and feed efficiency well past the normal age for weaning. In addition, they began to put on excess fat around …

Zipaterol Supplementation Improved Indicators Of Well-Being, But Not Growth In Heat-Stressed Red Angus Steers, Pablo C. Grijalva, Duarte E. Diaz, Samuel R. Garcia, Ty B. Schmidt, Jessica Lynn Petersen, Dustin T. Yates

Nebraska Beef Cattle Reports

The effects of zilpaterol (β2 agonist) supplementation on respiration rate, body temperature, growth, and carcass traits were studied in chronically heat-stressed feedlot steers. Through a collaborative partnership with the University of Arizona, Red Angus steers were heat stressed (90-105^oF) and fed zilpaterol-supplemented rations (Thermo-neutral control steers were pair-fed to the average feed intake of heat-stressed steers) for 30 days. Hot and cold carcass weights for heat-stressed cattle were 3% less than for controls, despite equal feed intake. Zilpaterol did not affect growth but improved the heat stress-induced hyperventilation and elevated body temperatures. Lighter carcass weights in heat-stressed cattle …

Inflammatory Modulators Improve Daily Gain Of Heat-Stressed Wethers, Micah S. Most, Pablo C. Grijalva, Dustin T. Yates

Nebraska Beef Cattle Reports

Inflammation during heat stress may mediate poor growth in livestock. The effects of anti-imflammatory treatments on muscle growth during chronic heat stress were evaluated by using meat lambs as a smaller cheaper model for feedlot steers. Wethers were maintained in normal (75^oF) or heat stress (105^oF for 12 hours/day, 85^oF for 12 hours/day) environments for 30 days and received dexamethasone injections every 3 days, oral fish oil supplementation twice daily, or no intervention. Growth was tracked and muscles were weighed when harvested afterward. Dexamethasone and fish oil both increased average daily gain over the …

Impact Of Cow Size On Economic Profitability In Cow-Calf And Feedlot Production Systmes, Robert L. Ziegler, Elliott James Dennis, J. A. Musgrave, T. L. Meyer, R. Funston, Kathryn J. Hanford, J. C. Macdonald, J. Travis Mulliniks

Nebraska Beef Cattle Reports

This study retrospectively evaluated the effect of cow size on profitability in the cow-calf segment and retained ownership of steer calves in the feedlot. Data were collected between 2005 to 2017 from the cowherd at Gudmundsen Sandhills Laboratory. From these data, two separate herds were assumed, one consisting of small-sized (1,000 lb) cows and another consisting of large-sized cows (1,220 lb) for the GSL cowherd. Larger cows weaned larger calves and produced heavier carcass weights of steers at slaughter. However, smaller cows generated more total pounds of output throughout the entire production system based on more calves retained in feedlots …

Statistics Used In The Nebraska Beef Cattle Report And Teir Purpose, University Of Nebraska-Lincoln

Nebraska Beef Cattle Reports

Te purpose of beef cattle and beef product research at UNL is to provide reference information that represents the various populations (cows, calves, heifers, feeders, carcasses, retail products, etc) of beef production. Obviously, the researcher cannot apply treatments to every member of a population; therefore he/she must sample the population. Te use of statistics allows the researcher and readers of the Nebraska Beef Cattle Report the opportunity to evaluate separation of random (chance) occurrences and real biological effects of a treatment. Following is a brief description of the major statistics used in the beef report. For a more detailed description …

Economics Of Grazing Calves On Oats Planted After Corn Silage In Eastern Nebraska, Kallie J. Calus, Mckenna M. Brinton, Benjamin H. Hansen, Kristen M. Ulmer, Zachery E. Carlson, Fred H. Hilscher, M. E. Drewnoski, Jay Parsons, J. C. Macdonald

Nebraska Beef Cattle Reports

A five-year study (2015–2019) evaluated profitability of planting and grazing an oats cover crop after early harvested corn silage in eastern Nebraska. Oats were typically planted in the first week of September. Stocking rate ranged from 1.7 to 0.6 steers per acre and was based on oats biomass. Calves were turned out in early November each year and allowed to graze until oats biomass or weather limited intake. Grazing period ranged from 30 to 69 days. Average daily gain of calves ranged from 3.35 to 1.29 pounds with an average of 1.97 pounds. Shortened grazing seasons in some years were …

Model Definition For Genetic Evaluation Of Purebred And Crossbred Lambs Including Heterosis, Napoleón Vargas Jurado, David R. Notter, Joshua B. Taylor, Daniel J. Brown, Michelle R. Mousel, Ronald M. Lewis

Department of Animal Science: Faculty Publications

Crossbreeding is a common practice among commercial sheep producers to improve animal performance. However, genetic evaluation of U.S. sheep is performed within breed type (terminal sire, semi-prolific, and western range). While incorporating crossbred records may improve assessment of purebreds, it requires accounting for heterotic and breed effects in the evaluation. The objectives of this study were to: 1) describe the development of a paternal composite (PC) line, 2) determine the effect of direct and maternal heterosis on growth traits of crossbred lambs, 3) estimate (co)variance components for direct and maternal additive, and uncorrelated maternal environmental, effects, and 4) provide an …

Interaction Of Urea With Frequency And Amount Of Distillers Grains Supplementation For Growing Steers On A High Forage Diet, Haley F. Linder, Josh E. Sebade, Zac E. Carlson, Hannah C. Wilson, Tyler J. Spore, Mary E. Drewnoski, Jim C. Macdonald

Department of Animal Science: Faculty Publications

Two studies were conducted to determine interactions of urea inclusion to a dried distillers grains plus solubles (DDGS; 29.4% crude protein, 5.48% ether extract) supplement fed at two amounts and two frequencies to steers on a high forage diet. In Exp. 1, 120 (247 kg; SD = 20) steers were fed individually for 84 d. Steers received ad libitum grass hay (6.8% crude protein) and one of eight treatments. Treatment design was a 2 × 2 × 2 factorial. Supplement was fed daily or three times per week, amount of supplement fed was 6.36 kg dry matter (DM)/week [0.37% body …