Meat Science Commons^™

Comparison Of Real-Time Pcr And Elisa-Based Methods For The Detection Of Beef And Pork In Processed Meat Products, Adam T. Perestam, Kayleigh K. Fujisaki, Omar Nava, Rosalee S. Hellberg

Food Science Faculty Articles and Research

Two commonly used methodologies for species detection within processed meat products are real-time polymerase chain reaction (PCR), a DNA-based method, and enzyme-linked immunosorbent assay (ELISA), a protein-based method. In this study, a real-time PCR assay was compared to a commercial ELISA kit based on sensitivity, specificity, agreement among duplicate samples, cost, time, and ease of use. Fifteen reference samples containing known percentages (0.1–99.9%, w/w) of pork and beef were analyzed in duplicate using both methods. Thirty commercial products, including sausages, pet treats, and canned meats, were also tested in duplicate with each method. Reference sample analysis showed real-time PCR was …

Use Of The Mitochondrial Control Region As A Potential Dna Mini-Barcoding Target For The Identification Of Canned Tuna Species, Jacquelyn K. Mitchell, Rosalee S. Hellberg

Food Science Faculty Articles and Research

In this study, a DNA mini-barcoding methodology was developed for the differentiation of species commonly found in canned tuna. Primers were designed to target a 236-base pair (bp) fragment of the mitochondrial control region (CR) and a 179-bp fragment of the first internal transcribed spacer region (ITS1). Phylogenetic analysis revealed the ability to differentiate 13 tuna species on the basis of the CR mini-barcode, except in a few cases of species introgression. Supplementary use of ITS1 allowed for differentiation of introgressed Atlantic bluefin tuna (Thunnus thynnus) and albacore tuna (Thunnus alalunga), while differentiation of introgressed Atlantic …

A Life Cycle Analysis Of Land Use In Us Pork Production, Greg Thoma, Marty Matlock, Ben Putman, Jasmina Burek

Food Systems

The goal of this study was to analyze land use in the production of US pork using Life Cycle Assessment (LCA). LCA is a comprehensive methodology for quantitatively analyzing potential environmental impacts associated with complex systems. Identification of processes contributing to high environmental impacts often highlights opportunities for gains in efficiency, which can increase the profitability and sustainability of US pork. The environmental impact category analyzed in this assessment was land use. After reviewing existing information regarding land use in agriculture and livestock production, analysis for US pork production was performed at two scales: cradle-to-grave and cradle-to-farm gate. The cradle-to-grave …

Production And Economic Efficiencies Of Intensified Cow–Calf Production Management Systems, Jason M. Warner

Department of Animal Science: Dissertations, Theses, and Student Research

The objective of four experiments conducted was to evaluate production and economic efficiencies of intensified cow-calf production management systems. The first experiment tested the effect of calf age at weaning on cow and calf performance and feed utilization at 2 locations. Body weight change from early to conventional weaning time was greater for early-weaned cows. Cow BCS and conception rates were not impacted by weaning. Calf BW at conventional weaning time was greater for conventionally-weaned calves than early-weaned calves at ARDC, but greater for early-weaned than conventionally-weaned calves at PHREC. Calf ADG per unit of total feed energy intake was …

Failure Of A Heterologous Recombinant Sca5/Ompb Protein-Based Vaccine To Elicit Effective Protective Immunity Against Rickettsia Rickettsii Infections In C3h/Hen Mice, Sean P. Riley, Marissa M. Cardwell, Yvonne G. Chan, Ludovic Pruneau, Fabio Del Piero, Juan J. Martinez

Faculty Publications

Spotted fever group (SFG) rickettsial species are obligate intracellular tick-borne pathogens that are responsible for important human diseases. Previous reports have demonstrated the feasibility of using recombinant surface cell antigen Sca5/OmpB to elicit protective immunity against homologous challenges using murine models of Mediterranean spotted fever and Rocky Mountain spotted fever. In addition, the feasibility of generating cross-protective immunity against related rickettsial species has also been established, but the molecular basis for these phenomena was not explored. Here, we demonstrate that vaccination of C3H/HeN mice with a recombinant OmpB domain derived from Rickettsia conorii induced high titer humoral immune responses that …

Dietary Lecithin Supplementation Can Improve The Quality Of The M. Longissimus Thoracis, Darryl N. D'Souza, Bronwyn L. Blake, Ian H. Williams, Bruce P. Mullan, Dave Pethick, Frank R. Dunshea

Animal production and livestock research articles

Forty crossbred (Large White × Landrace × Duroc) female pigs (16.4 kg ± 0.94 kg) were used to investigate the effect of dietary lecithin supplementation on growth performance and pork quality. Pigs were randomly allocated to a commercial diet containing either 0, 3, 15 or 75 g lecithin/kg of feed during the grower and finisher growth phase. Pork from pigs consuming the diets containing 15 g and 75 g lecithin/kg had lower hardness (P < 0.001) and chewiness (P < 0.01) values compared to the controls. Dietary lecithin supplementation at 75 g/kg significantly increased (P < 0.05) the linoleic acid and reduced (P < 0.05) the myristic acid levels of pork compared to the control and the 3 g/kg and 15 g/kg lecithin supplemented treatments. Pigs fed the 75 g/kg lecithin supplemented diet had lower plasma cholesterol (P < 0.05) at slaughter compared to pigs fed the control diet and the 3 g/kg and 15 g/kg lecithin supplemented treatments. These data indicate that dietary lecithin supplementation has the potential to improve the quality attributes of pork from female pigs.

A Tail Of Two Phages: Genomic And Functional Analysis Of Listeria Monocytogenes Phages Vb_Lmos_188 And Vb_Lmos_293 Reveal The Receptor-Binding Proteins Involved In Host Specificity, Aidan Casey, Kieran Jordan, Horst Neve, Aidan Coffey, Olivia Mcauliffe

Department of Biological Sciences Publications

The physical characteristics of bacteriophages establish them as viable candidates for downstream development of pathogen detection assays and biocontrol measures. To utilize phages for such purposes, a detailed knowledge of their host interaction mechanisms is a prerequisite. There is currently a wealth of knowledge available concerning Gram-negative phage-host interaction, but little by comparison for Gram-positive phages and Listeria phages in particular. In this research, the lytic spectrum of two recently isolated Listeria monocytogenes phages (vB_LmoS_188 and vB_LmoS_293) was determined, and the genomic basis for their observed serotype 4b/4e host-specificity was investigated using comparative genomics. The late tail genes of these …

Effect Of Supplemental Trace Mineral (Zinc, Copper, And Manganese) Source On Growth Performance, Morbidity, And Trace Mineral Status In Beef Cattle, Anthony Ryan

Graduate Theses and Dissertations

A series of studies were conducted to determine the effect of supplemental trace mineral source on growth performance, morbidity, and trace mineral status in growing beef cattle. The first experiment evaluated supplemental trace minerals from sulfate, organic, or hydroxy sources on growth performance and morbidity. Crossbreed beef calves were assigned to 1 of 3 treatments consisting of supplemental zinc (360 mg/d), copper (125 mg/d), and manganese (200 mg/d) from inorganic, organic, or hydroxy sources fed daily over a 42 to 45-d backgrounding phase. After removal of chronic and dead calves from the data set, trace mineral source had no effect …

Influence Of Steroids And Gonadotropins On Reproduction In Beef Cattle, Thomas L. Devine

Graduate Theses and Dissertations

The objective of this thesis was to evaluate the influence of steroids and gonadotropins on reproduction in beef cattle. In experiment 1, beef heifers were used to determine the influence of growth-promoting implants on growth, reproductive development, estrous behavior, and pregnancy rate. Heifers were assigned to 1 of 4 implant treatment groups: control (CON); trenbolone acetate (TBA); trenbolone acetate plus estradiol (TBA+E2) or zeranol (ZER). Heifers were implanted once, A.I. and exposed to bull during this experiment. Body weight, BCS, HH, RTS, estrous behavior and pregnancy data were collected throughout this experiment. Average daily gain of heifers was greater for …

Balking Incidence And Behavioral Responses During Handling In Fed Cattle With Genetic And Carcass Implications, Michelle Lynn Thomas

Graduate Theses and Dissertations

Temperament in cattle is often defined as the reactivity to human handling or novel environments. Temperament differences have been shown among breed-type categories, within breed types, among crossbreds, and between sexes. Temperament tests are typically completed at weaning time on beef cattle, and rarely on fed Holstein steers for beef production. Balking behavior, or cease in forward motion, in the cattle working facility can pose welfare issues as the electric prod use to coerce movement is implemented. Three observational field projects were designed to evaluate balking behavior incidence in unknown breed-types at the processing plant, Holstein steers in the feedlot …

Letter From The Dean, Lalit Verma

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Risk Assessment And Decision-Making For Genetically Modified Foods, Aynsley Kellow

RISK: Health, Safety & Environment (1990-2002)

The author examines concerns about genetically modified foods and how those concerns have developed into policy responses markedly different in Europe than in the United States.

Controlling Sheep Meat Disorders, Michael Paton Dr

Bulletins 4000 -

No abstract provided.

Other On-Station Activities For Wool Pastoralists, Mark Stevens

Journal of the Department of Agriculture, Western Australia, Series 4

The impact of low wool prices has been most severe on specialist wool growers who have little scope for diversification, particularly those in the pastoral area.

Although there are limited opportunities for new enterprises on all pastoral stations, individual pastoralists are examining other on-station activities to determine which ones might be suitable. In doing so, they are evaluating:

• location (proximity to a major highway or population centre);

• natural attractions (coast, gorges, river, wildflowers);

• natural resources (native fauna and flora); and

• water supply (quantity and reliability of good quality water).

Veterinary Consultancy Boosts Piggery Profits, Ashley Mercy

Journal of the Department of Agriculture, Western Australia, Series 4

A pilot Pig Herd Health Management Scheme co-ordinated by the Department of Agriculture has stimulated much increased involvement of veterinarians in the Western Australian pig industry. Co-ordinated by Ashley Mercy, principal veterinary officer (epidemiology) horn 1985 to 1989, the scheme demonstrated the value of having regular visits from veterinary consultants and in implementing programs to prevent disease and production problems occurring in herds.

The Western Australian Pig Health Monitoring Scheme, Ashley Mercy

Journal of the Department of Agriculture, Western Australia, Series 4

Over 30,000 slaughter pigs from 245 Western Australian piggeries have been examined for the presence of 16 diseases since the Western Australian Pig Health Monitoring Scheme (PHMS) started in January 1987. The scheme was developed by the author in conjunction with Dr Chris Brennan, a pig veterinary consultant. Monitoring of slaughter pigs is an important part of providing an effective veterinary service to commercial piggeries. It can be used in accreditation schemes and to help in certifying herds free of particular disease

Ec75-219 Nebraska Swine Report, William Ahlschwede, T. E. Socha, Alfonso Torres-Medina, A. J. Lewis, P. J. Cunningham, Dwane R. Zimmerman, E. R. Peo Jr., Larry K. Mcmullen, Bobby D. Moser, D. L. Ferguson, Phillip H. Grabouski, Murray Danielson, Charles W. Francis, L. F. Elliott, J. A. Deshazer, Roger W. Mandigo, W. J. Goldner, R. D. Fritschen, Gary Zoubek

University of Nebraska-Lincoln Extension: Historical Materials

This 1975 Nebraska Swine Report was prepared by the staff in Animal Science and cooperating departments for use in the Extension and Teaching programs at the University of Nebraska-Lincoln. Authors from the following areas contributed to this publication: Swine Nutrition, swine diseases, pathology, economics, engineering, swine breeding, meats, agronomy, and diagnostic laboratory. It covers the following areas: breeding, disease control, feeding, nutrition, economics, housing and meats.

Ultrasonic Testing : An Aid To Better Breeding, B M. Goss

Journal of the Department of Agriculture, Western Australia, Series 4

THE ultrasonic tester was originally developed as a metal flaw-detector for industrial purposes, and its usefulness for making carcass measurements on the live pig was first demonstrated about 10 years ago.

The Baconer Carcass Competition : A Guide To Better Quality Pigs, P Mcnamara

Journal of the Department of Agriculture, Western Australia, Series 4

TO PROMOTE the ever-increasing trade with Britain and encourage quality production, the Australian Meat Board sponsored the 1937 All Australian Baconer and Pork carcass competition.

Cc144 Adjusting To Drought...Why Cull Your Herd Now?, K. C. Fouts, Philip Cole, Paul Guyer, C. W. Nibler, P. A. Henderson

University of Nebraska-Lincoln Extension: Historical Materials

Extension Circular CC144 This circular is about adjusting to drought and why to cull your herd now.

Carcass Quality Of Inbred And Crossbred Uark Poland China Swine, M. C. Heck, E. S. Ruby

Journal of the Arkansas Academy of Science

No abstract provided.