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The University of Maine

Honors College

Biology

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Testing The Effect Of Grapefruit Seed Extract (Gfse) On The Mastitsis-Causing Alga, Prototheca, Kendra Janelle Macdonald May 2013

Testing The Effect Of Grapefruit Seed Extract (Gfse) On The Mastitsis-Causing Alga, Prototheca, Kendra Janelle Macdonald

Honors College

Mastitis is an inflammation of the mammary gland that costs the United States dairy industry between $1.7 and $2 billion annually [9]. Approximately 97% of mastitis infections are caused by bacteria, which can be treated with antibiotics [2]. However, mastitis infections can be caused by the alga, Prototheca. Specifically, it is P. zopfii (genotype 2) and P. blaschkeae that have been found in cows with mastitis [7]. There are currently no approved treatments for protothecal mastitis. The following experiments a tested the effects of grapefruit seed extract (GFSE) on P. zopfii (genotype 2) using spectrophotometry and plating techniques. GFSE was …