Aquaculture and Fisheries Commons^™

Patterns Of Larval Atlantic Croaker Ingress Into Chesapeake Bay, Usa, Jason J. Schaffler, Christian S. Reiss, Cynthia M. Jones

OES Faculty Publications

We compared ingress patterns of Atlantic croaker Micropogonias undulatus larvae into Chesapeake Bay, USA, with published ingress patterns through barrier island inlets, the accepted model for larval fish ingress. This model asserts that larvae ingress on night flood tides at the flooddominated side of the inlet and at all depths. At the Chesapeake Bay mouth and in the adjacent coastal waters, we compared the distribution of abundance, size, age, and growth rates of croaker prior to ingress, In contrast to the barrier island inlet model, croaker larvae were more abundant at depth than closer to the surface regardless of location. …

Is Seagrass An Important Nursery Habitat For The Caribbean Spiny Lobster, Panulirus Argus, In Florida?, Donald C. Behringer, Mark J. Butler Iv, William Herrnkind, John H. Hunt, Charles A. Acosta, William C. Sharp

Biological Sciences Faculty Publications

Caribbean spiny lobster (Panulirus argus) settle preferentially in macroalgal-covered hard-bottom habitat, but seagrass is more prevalent in Florida (United States) and the Caribbean, so even low settlement of lobsters within seagrass could contribute substantially to recruitment if post-settlement survival and growth were high. We tested the role of seagrass and hard-bottom habitats for P. argus recruitment in three ways. We first explored possible density-dependent regulation of early benthic juvenile lobster survival within cages deployed in seagrass and hard-bottom habitats. Second, we compared settlement and survival of P. argus in both habitats, by comparing the recovery of microwire-tagged early …

Regional Characterisation Of Hard-Bottom Nursery Habitat For Juvenile Caribbean Spiny Lobster (Panulirus Argus) Using Rapid Assessment Techniques, Rodney D. Bertelsen, Mark J. Butler Iv, William F. Herrnkind, John H. Hunt

Biological Sciences Faculty Publications

Shallow, hard-bottom habitat constitutes approximately 30% of the coastal waters of south Florida, United States, yet it is a chronically understudied feature of the marine seascape in this region. In this study, we characterised the general biogeographic and structural features of shallow benthic hard-bottom communities in the Florida Keys, and related those to the abundance of juvenile Caribbean spiny lobster (Panulirus argus), the target of one of Florida's most economically valuable fisheries. We used rapid assessment techniques to survey more than 100 hard-bottom sites in the Florida Keys to estimate the percentage bottom coverage of vegetation (seagrass and …

Potential Effects Of Catastrophic Cyanobacteria Blooms On Caribbean Spiny Lobster Population Dynamics In Florida Bay Usa, Donald C. Behringer, Mark J. Butler Iv

Biological Sciences Faculty Publications

No abstract provided.

Patterns Of Spiny Lobster (Panulirus Argus) Postlarval Recruitment In The Carribbean: A Crtr Project, Mark J. Butler Iv, Angela M. Mojica, Eloy Sosa-Cordero, Marines Millet, Paul Sanchez-Navarro, Miguel A. Maldonado, Juan Posada, Bladimir Rodriguez, Carlos M. Rivas, Adrian Oviedo, Marcio Arrone, Martha Prada, Nick Bach, Nilda Jimenez, Maria Del Carmen Garcia-Rivas, Kirah Forman, Donald C. Behringer Jr., Thomas Matthews, Claire Paris, Robert Cowen

Biological Sciences Faculty Publications

As part of the Coral Reef Targeted Research (CRTR) Program, a partnership between the Global Environment Facility and the World Bank, our research team examined the recruitment patterns of Caribbean spiny lobster (Panulirus argus) postlarvae among regions in the Caribbean, with a particular focus on Mesoamerica. Our goal was to collect comparable information on postlarval supply among regions and to provide data to test predictions of connectivity generated from a coupled biophysical oceanographic model of lobster larval dispersal. Here we present the results of the postlarval recruitment monitoring program. We monitored the catch of postlarvae on Witham-style collectors …

Peptide Hydrolysis And The Uptake Of Dipeptides By Phytoplankton, Margaret R. Mulholland, Cindy Lee

OES Faculty Publications

Rates of peptide hydrolysis (using the fluorescent substrate, lucifer yellow anhydride-labeled tetra-alanine) and dipeptide uptake (using dually labeled, ¹⁵N and ¹³C, dialanine) were measured in phytoplankton cultures and in natural populations during algal blooms dominated by one or two taxa. During most sampling events, both peptide hydrolysis and dipeptide uptake were greatest in the size fraction containing the dominant phytoplankter, suggesting that phytoplankton contribute substantially to or may even dominate observed extracellular peptide hydrolysis and dipeptide uptake in the environment. These are the first data suggesting that dipeptides may be taken up directly by phytoplankton and this may …

Spatial Variation In Otolith Chemistry Of Atlantic Croaker Larvae In The Mid-Atlantic Bight, Jason J. Schaffler, Christian S. Reiss, Cynthia M. Jones

OES Faculty Publications

Larval Atlantic croaker Micropogonias undulatus (1 to 7 mm in standard length) were collected on the east coast of the United States from North Carolina to Delaware during 2000. We defined 3 water-mass boundaries for potential groups of spawning Atlantic croaker using temperature and salinity measured at each sampling station. We tested the hypothesis that distinct otolith chemistries existed among 3 groups of larval Atlantic croaker collected from these water masses using solution-based inductively coupled plasma-mass spectrometry. Multivariate analysis of variance indicated that otolith chemistry differed significantly among water masses. Using a quadratic discriminant function, we were able to correctly …

Mycobacteriosis-Associated Mortality In Wild Striped Bass (Morone Saxatilis) From Chesapeake Bay, Usa, D. T. Gauthier, R. J. Latour, D. M. Heisey, C. F. Bonzek, J. Gartland, E. J. Burge, W. K. Vogelbein

Biological Sciences Faculty Publications

The striped bass (Morone saxatilis) is an economically and ecologically important finfish species along the Atlantic seaboard of the United States. Recent stock assessments in Chesapeake Bay (USA) indicate that non-fishing mortality in striped bass has increased since 1999, concomitant with very high (>50%) prevalence of visceral and dermal disease caused by Mycobacterium spp. Current fishery assessment models do not differentiate between disease and other components of non-fishing mortality (e. g., senescence, predation); therefore, disease impact on the striped bass population has not been established. Specific measurement of mortality associated with mycobacteriosis in wild striped bass is …

Otolith Chemistry Indicates Population Structuring By The Antarctic Circumpolar Current, Julian R. Ashford, Cynthia M. Jones, Eileen E. Hofmann, Inigo Everson, Carlos A. Moreno, Guy Duhamel, Richard Williams

CCPO Publications

Large-scale transport of seawater in ocean currents may generate spatially complex population structure through the advection of life stages of marine fish species. To test this, we compared the chemistry of otolith nuclei from Patagonian toothfish (Dissostichus eleginoides), presently managed as spatially discrete Populations corresponding to fishing management areas along the Antarctic Circumpolar Current (ACC), which transports water eastward around the Southern Ocean. The chemistry of otolith nuclei, laid down during early life, differed significantly between fishing areas Off South America and the Antarctic and between some Antarctic areas. However, we found significant discrepancies from expectation for a …

The Use Of Extractable Lipofuscin For Age Determination Of Crustaceans: Reply To Sheehy (2008), H. Rodger Harvey, David H. Secor, Se-Jong Ju

OES Faculty Publications

The heterogeneous mixture of metabolic by-products termed lipofuscin (LF) or age pigments has long been known to accumulate in post mitotic cells with increasing age. In crustaceans several approaches have been developed over the years to track LF accumulation and provide a proxy for chronological age. Histological approaches have been traditionally used for LF determination of crustaceans (e.g. Sheehy et al. 1994, Mar Biol 121:237-245), but over the last decade extraction approaches for neural tissues have been introduced (Ju et al. 1999, Mar Ecol Prog Ser 185:171-179) and tested (Ju et al. 2001, Mar Ecol Prog Ser 224:197-205; 2003, Fish …

Limb Regeneration After Multiple Autotomy And Coxae Removal In The Blue Crab Callinectes Sapidus, Jennifer Ambler

OES Theses and Dissertations

This study investigated limb regeneration in the juvenile blue crab, Callinectes sapidus, specifically the role of the coxa and pedal nerve innervation of the regenerative limb bud over time. The coxa is a leg segment that has been defined as the source of positional, cellular, and neuronal information needed for limb regeneration in brachyuran crabs. This study indicates that removal of coxae under regenerative and non-regenerative conditions did not deter limb regeneration. The coxa is not the exclusive location of limb regenerative information since limbs re-grew with normal positional and functional arrangement in 86% of crabs showing regeneration at removal …

Molecular Phylogenetics Of Perciform Fishes Using The Nuclear Recombination Activating Gene 1, Andrew R. Mahon

Biological Sciences Theses & Dissertations

The order Perciformes contains one-third of all extant fishes in twenty different suborders and over 10,000 species. Few systematic investigations have been performed on this large group of fishes at the suborder level and their evolutionary history is widely recognized as problematic. This dissertation presents three studies: a molecular phylogenetic analysis of the putative suborders of the order Perciformes, an analysis of interrelationships of the families of the perciform suborder Percoidei, and a multi-gene investigation of the percoid superfamily Sparoidea.

The taxa sampled in this dissertation represent one of the most inclusive molecular datasets, to date, testing the monophyly of …

Review Of The Crevalle Jacks, Caranx Hippos Complex (Teleostei: Carangidae), With A Description Of A New Species From West Africa, William F. Smith-Vaniz, Kent E. Carpenter

Biological Sciences Faculty Publications

The Caranx hippos species complex comprises three extant species: crevalle jack (Caranx hippos) (Linnaeus, 1766) from both the western and eastern Atlantic oceans; Pacific crevalle jack (Caranx caninus) Günther,1868 from the eastern Pacific Ocean; and longfin crevalle jack (Caranx fischeri) new species, from the eastern Atlantic, including the Mediterranean Sea and Ascension Island. Adults of all three species are superficially similar with a black blotch on the lower half of the pectoral fin, a black spot on the upper margin of opercle, one or two pairs of enlarged symphyseal canines on the lower jaw, …

Otolith Chemistry Reflects Frontal Systems In The Antarctic Circumpolar Current, J. R. Ashford, A. I. Arkhipkin, C. M. Jones

OES Faculty Publications

Pronounced environmental trends across fronts suggest that the otolith chemistry of oceanic fish can resolve zones on either side, promoting application to population questions at similar spatial scales. Trace and minor elements laid down immediately prior to capture - along the edges of otoliths from Patagonian toothfish Dissostichus eleginoides - discriminated frontal zones in the Antarctic Circumpolar Current in the Southwestern Atlantic Ocean. Mean values differentiated sampling areas by up to 2.6 standard deviations, suggesting: (1) otolith Mg/Ca enrichment related to fish activity around the Burdwood Bank; (2) Mn/Ca enrichment associated with South America; (3) Sr/Ca linked to the presence …

A Sex-Linked Allele, Autosomal Modifiers And Temperature-Dependence Appear To Regulate Melanism In Male Mosquitofish (Gambusia Holbrooki), Lisa Horth

Biological Sciences Faculty Publications

About 1% of male mosquitofish (Gambusia holbrooki) express melanic (mottled-black) body coloration, which differs dramatically from the wild-type, silvery-gray coloration. Here, I report on the genetic inheritance pattern of melanic coloration, which indicates Y-linkage, and at least one autosomal modifier. Phenotypic expression of melanism is also affected by temperature. Expression is constitutive ( temperature insensitive) in some populations, inducible ( temperature sensitive) in others. Constitutive and inducible expression occur among geographically proximal populations. However, males from any single population demonstrate the same constitutive or inducible expression pattern as one another. The F1 males from inter-population crosses demonstrate temperature-related …

A Decade Of Changes For Virginia Freshwater Aquaculture (1993-2003), Scott H. Newton

Virginia Journal of Science

Aquaculture surveys were designed and initiated along with the writing of the State Aquaculture Plan during 1993 to 1995. The first Virginia Aquaculture Survey, conducted for the production year 1993, established the status of both freshwater and marine commercial industries. Subsequent surveys were conducted to trace industry developments relevant to production aspects and economic impacts. Survey data from Virginia Agricultural Statistics Service Reports for 1993, 1995, 1997, and 2003 summarize a decade of Virginia freshwater aquaculture information. Significant changes in sales among the principal aquaculture species occurred over the 10-year period. The 1993 freshwater aquaculture value of $2.8 million increased …

Detection Of Panulirus Argus Virus 1 (Pav1) In The Caribbean Spiny Lobster Using Fluorescence In Situ Hybridization (Fish), Caiwen Li, Jeffrey D. Shields, Hamish J. Small, Kimberly S. Reece, Carmony L. Hartwig, Roland A. Cooper, Robert E. Ratzlaff

Biological Sciences Faculty Publications

Panulirus argus Virus 1 (PaV1) is the first virus known to be pathogenic to a wild lobster. It infects the Caribbean spiny lobster P. argus from the Florida Keys, and has a predilection for juveniles. The monitoring of the virus in wild populations and study of its behavior in the laboratory require the development of reliable diagnostic tools. A sensitive and specific fluorescence in situ hybridization (FISH) assay was developed for detection of PaV1. The lower detection limit using a 110 bp DNA probe in a dot-blot hybridization for PaV1 DNA was 10 pg of cloned template PaV1 DNA and …

Substituting Otoliths For Chemical Analyses: Does Sagitta = Lapillus?, Nathan G. Smith, Cynthia M. Jones

OES Faculty Publications

Chemical analysis of fish otoliths has become an important technique in fisheries science with widespread applications. Most research up to this point has focused predominantly on sagittal otoliths, but the underlying assumptions may also apply to lapilli. The goal of this study was to determine whether lapilli and sagittae have the same otolith chemistry and whether one can be substituted for the other for solution-based chemical analysis in wild-captured fish. We compared the stable isotope chemistry (δ13C and δ18O) of paired sagittae and lapilli of juvenile spotted seatrout Cynoscion nebulosus collected from Chesapeake Bay seagrass beds in 2002. …

Comparison Of Aquatic Life Depicted In Illustrations And Plaster Casts Of The Punt Relief From The Temple Of Hatshepsut At Deir El-Bahari, Emily Lord, Eugene G. Maurakis

Virginia Journal of Science

The primary objective of this study is to document differences between image characteristics of two sources (illustrations in Naville, 1898; and images in the cast of the relief at Virginia Museum of Fine Arts (VMFA) of the Punt relief from the temple of Hatshepsut at Deir El-Bahri. Our second is to compare cast images to photographs of the original relief. Characteristics of 30 species in the illustrations were described and compared to descriptions of the corresponding 30 species photographed from the cast at VMFA. The number of differences and similarities were recorded for each pair of corresponding icons and used …

Body-Induced Vortical Flows: A Common Mechanism For Self-Corrective Trimming Control In Boxfishes, Ian K. Bartol, Morteza Gharib, Paul W. Webb, Daniel Weihs, Malcolm S. Gordon

Biological Sciences Faculty Publications

Boxfishes (Teleostei: Ostraciidae) are marine fishes having rigid carapaces that vary significantly among taxa in their shapes and structural ornamentation. We showed previously that the keels of the carapace of one species of tropical boxfish, the smooth trunkfish, produce leading edge vortices (LEVs) capable of generating self-correcting trimming forces during swimming. In this paper we show that other tropical boxfishes with different carapace shapes have similar capabilities. We conducted a quantitative study of flows around the carapaces of three morphologically distinct boxfishes (spotted boxfish, scrawled cowfish and buffalo trunkfish) using stereolithographic models and three separate but interrelated analytical …

Can Otolith Elemental Signatures Record The Capture Site Of Patagonian Toothfish (Dissostichus Eleginoides), A Fully Marine Fish In The Southern Ocean?, J. R. Ashford, C. M. Jones, E. Hofmann, I. Everson, C. Moreno, G. Duhamel, R. Williams

CCPO Publications

Otolith chemistry has been successfully used to reconstruct the environmental history experienced by estuarine-dependent teleost fish, including movement between estuaries and coastal areas. However, application has been more limited in species exposed exclusively to oceanic waters, where gradients in physical and chemical properties are less extreme. To test whether otolith elemental signatures record spatial information in an oceanic species, we sampled otoliths from Patagonian toothfish (Dissostichus eleginoides) and used an inductively coupled plasma mass spectrometer (ICP-MS) coupled to a laser ablation system to target the outer otolith edges corresponding to the period immediately before capture. Using multivariate analysis …

Feeding Ecology Of Juvenile Spiny Lobster, Panulirus Interruptus, On The Pacific Coast Of Baja California Sur, Mexico, Verónica Castañeda‐Fernández‐De‐Lara, Elisa Serviere‐Zaragoza, Sergio Hernández‐Vázquez, Mark J. Butler Iv

Biological Sciences Faculty Publications

Many aspects of the early life history of the red lobster Panulirus interruptus are little known, including the relationship between habitat structure, food resource availability, and nutrition of juveniles. We investigated the spatial and temporal differences in food intake, diet composition, and nutritional condition of juveniles at two sites along the Pacific coast of the Baja California Peninsula (Mexico) with contrasting oceanographic and biological conditions. One site (Arvin) is located inside a protected bay, Bahia Tortugas, where the waters are cooler and temperate seagrasses and macroalgae are the dominant benthic flora. The second site (Queen) in Bahia Sebastian Vizcaino was …

Chemistry Of Surface Waters: Distinguishing Fine-Scale Differences In Sea Grass Habitats Of Chesapeake Bay, Emmanis Dorval, Cynthia M. Jones

OES Faculty Publications

We tested the hypothesis that the physical and chemical processes acting in sea grass habitats of the lower Chesapeake Bay are spatially structured and that dissolved elemental chemistry of sea grass-habitat surface waters have their own unique identity. We sampled surface waters from July to September 2001 in five sea grass habitats of the lower bay: Potomac, Rappahannock, York, Island (Tangier-Bloodsworth), and Eastern Shore. Dissolved Mg, Mn, Sr, and Ba concentrations were measured by sector field inductively coupled plasma-mass spectrometry. As expected, Mg, Sr, and Ba exhibited conservative behavior, but Mn exhibited nonconservative behavior along the salinity gradient. Spatial differences …

Biophysical Mechanisms Of Larval Fish Ingress Into Chesapeake Bay, Jonathan A. Hare, Simon Thorrold, Harvey Walsh, Christian Reiss, Arnoldo Valle-Levinson, Cynthia Jones

OES Faculty Publications

Selective tidal stream transport is hypothesized as a dominant mechanism by which larvae of marine animals move through estuarine openings. For larvae moving from the shelf to estuarine habitats, selective tidal stream transport proposes that larvae are higher in the water column during flood tide and lower in the water column during ebb tide. Although a number of studies conclude that selective tidal stream transport is the mechanism responsible for larval ingress, few studies consider alternative mechanisms or consider passive explanations for tidal patterns in larval distributions. We examined the biophysical mechanisms responsible for larval ingress into Chesapeake Bay using …

A Modelling Study Of The Influence Of Environment And Food Supply On Survival Of Crassostrea Gigas Larvae, Eileen E. Hofmann, Eric N. Powell, Eleanor A. Bochenek, John M. Klinck

CCPO Publications

A biochemically based model was developed to simulate the growth, development, and metamorphosis of larvae of the Pacific oyster (Crassostrea gigas). The unique characteristics of the model are that it: (1) defines larvae in terms of their protein, neutral lipid, polar lipid, carbohydrate, and ash content; (2) tracks weight separately from length to follow larval condition; and (3) includes genetic variation in growth efficiency and egg quality to better simulate cohort population dynamics. The model includes parameterizations for filtration, ingestion, and respiration, which determine larval growth rate, and processes controlling larval mortality and metamorphosis. Changes in larval tissue …

Lagrangian Modelling Studies Of Antarctic Krill (Euphausia Superba) Swarm Formation, Eileen E. Hofmann, A. G. Edward Haskell, John M. Klinck, Cathy M. Lascara

CCPO Publications

A two-dimensional Lagrangian particle model was developed to examine the spatial distribution of Antarctic krill (Euphausia superba). The time-dependent location of particles, which represent krill individuals, is determined by random diffusion, foraging activity, and movement induced by the presence of neighbours. Foraging activity is based on prescribed food conditions and is such that krill swim slower and turn more frequently in areas of high food concentration. The presence or absence of neighbours either disperses krill, if the local concentrations become too dense, or coalesces krill, if concentrations become too dilute, respectively. Predation on krill is included and affects …

Ultrastructure Of Mycobacterium Marinum Granuloma In Striped Bass Morone Saxatilis, D. T. Gauthier, W. K. Vogelbein, C. A. Ottinger

Biological Sciences Faculty Publications

An emerging epizootic of mycobacteriosis currently threatens striped bass Morone saxatilis populations in Chesapeake Bay, USA. Several species of mycobacteria, including Mycobacterium marinum, species resembling M. avium, M. gordonae, M. peregrinum, M. scrofulaceum and M. terrae, and the new species M. shottsii have been isolated from diseased and healthy bass. In this study, we describe the ultrastructure of developing M. marinum granulomas in experimentally infected bass over a period of 45 wk. The primary host response to injected mycobacteria was formation of large macrophage aggregations containing phagocytosed bacilli. M. marinum were always contained within phagosomes. Close …

A New Pathogenic Virus In The Caribbean Spiny Lobster Panulirus Argus From The Florida Keys, Jeffrey D. Shields, Donald C. Behringer Jr.

Biological Sciences Faculty Publications

A pathogenic virus was diagnosed from juvenile Caribbean spiny lobsters Panulirus argus from the Florida Keys. Moribund lobsters had characteristically milky hemolymph that did not clot. Altered hyalinocytes and semigranulocytes, but not granulocytes, were observed with light microscopy. Infected hemocytes had emarginated, condensed chromatin, hypertrophied nuclei and faint eosinophilic Cowdry-type-A inclusions. In some cases, infected cells were observed in soft connective tissues. With electron microscopy, unenveloped, nonoccluded, icosahedral virions (182 +/- 9 nm SD) were diffusely spread around the inner periphery of the nuclear envelope. Virions also occurred in loose aggregates in the cytoplasm or were free in the hemolymph. …

Isolation And Characterization Of Mycobacteria From Striped Bass Morone Saxatilis From The Chesapeake Bay, Martha W. Rhodes, Howard Kator, Ilsa Kaattari, David Gauthier, Wolfgang Vogelbein, Christopher A. Ottinger

Biological Sciences Faculty Publications

Mycobacteriosis in striped bass Morone saxatilisof Chesapeake Bay, USA, was first diagnosed in 1997 based on the presence of granulomatous inflammation and acid-fast bacteria in skin and spleen. To confirm histopathology, bacteriological detection and identification of mycobacteria were begun using splenic tissue from fish with and without skin ulcerations. On the basis of initial studies using a variety of selective and nonselective media, decontamination, homogenization and incubation conditions, a simple and quantitative recovery method using aseptic necropsy of splenic tissue was developed. Optimal recovery was obtained by spread-plating homogenates on Middlebrook 7H10 agar with incubation for 3 mo at …

Age And Growth Of Scotia Sea Icefish, Chaenocephalus Aceratus, From The South Shetland Islands, M. La Mesa, J. Ashford, E. Larson, M. Vacchi

OES Faculty Publications

Samples of Chaenocephalus aceratus (Lönnberg) were collected during a trawl survey carried out around the South Shetland Islands in January–February 2002. Fish were caught by commercial bottom trawl fishing down to 500 m depth, using a stratified randomized sampling design. As observed in other recent surveys within the same area, C. aceratus represented one of the predominant species. Overall, 357 specimens ranging from 13 and 67 cm (TL) were selected for the present study. Ages were estimated by counting annuli present in the sagittal otoliths, exposed by grinding and polishing along their sagittal plane. To estimate the precision of age …