Animal Sciences Commons^™

Prevalence Of Toxoplasma Gondii In Sheep Meats Purchased From Retailstores In Central Anatolia, Turkey, Kader Yildiz, Fatma Çi̇ğdem Pi̇şki̇n, Armağan Erdem Ütük, Sami̇ Gökpinar

Turkish Journal of Veterinary & Animal Sciences

Toxoplasmosis is one of the most important foodborne parasitic diseases of humans. In particular, sheep muscles are significant sources of infection in the transmission of toxoplasmosis. Carnivorism is the most important transmission route for human populations. The aim of this study was to detect the prevalence of Toxoplasma gondii tissue cysts in sheep meats in retail stories of Turkey. A total of 250 boneless sheep meat samples were purchased from randomly selected retail stores in different locations of Ankara and Kırıkkale provinces of Turkey. The homogenized meat samples were centrifuged with Percoll dilutions. The tissue cysts were removed by pipette …

Determination Of Serum Cardiac Biomarkers And Plasma D-Dimer Levelsin Anemic Sheep With Babesiosis, Özlem Orunç Kilinç, Yaşar Göz, Nazmi̇ Yüksek, Yildiray Başbuğan, Ali̇ Bi̇lgi̇n Yilmaz, Ahmet Duran Ataş

Turkish Journal of Veterinary & Animal Sciences

In this study, the cardiac effects of anemia and venous thromboembolism (VTE), reportedly caused by the hemolyzed erythrocytes occurring in sheep with babesiosis, were investigated using cardiac markers and D-dimer (DD). The study included 34 sheep: 24 infected Akkaraman sheep (1-3 years old, diagnosed with babesiosis based on clinical and laboratory findings) and a control group of 10 noninfected healthy sheep of the same breed and age. Hematocrit (Hct) levels were measured in blood obtained from both groups. Cardiac troponin I (cTnI), creatine kinase-MB (CK-MB), and aspartate aminotransferase (AST) levels were measured in serum samples. In addition, the levels of …

Assessing The Impact Of Supplements, Food Aversions, And Silica On Medusahead Use By Sheep, Tyler Hamilton

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Ranchers, farmers, land managers, and resource management agencies have been plagued with various types of invasive weeds for decades, ultimately affecting millions of acres of viable wildlife habitat, grazing, and recreational opportunities. Invasive weeds often have nutritional contexts that negatively affect animals in some way, increasing the incentive to avoid consuming it by various animal species and domestic livestock. With the current amount of land infested with this weed, and the limited knowledge of the reason for avoidance, the underlying causes of livestock avoidance require in-depth and fundamental understanding.

In my MS program, I sought to explore the relationship of …

Comparative Digestibility By Cattle Versus Sheep: Effect Of Forage Quality, S. A. Soto-Navarro, R. Lancaster, C. Sankey, B. M. Capitan, B. P. Holland, L. A. Balstad, C. R. Krehbiel

Department of Animal Science: Faculty Publications

The objective was to determine the effect of forage quality on apparent total tract digestibility and ruminal fermentation in cattle versus sheep. Five yearling English crossbred (Hereford × Angus) steers (440.4 ± 35.6 kg of initial BW) and 5 yearling whiteface (Rambouillet × Columbia × Debouillet) wethers (44.4 ± 4.6 kg of initial BW), each fitted with a ruminal cannula, were randomly assigned to 1 of 3 forage sources within ruminant specie, and the study was conducted over 3 periods. For forage source, both animal and period served as the blocking factor with all forage sources represented once within each …

The Relationship Between Seropositivity And Tissue Cysts In Sheep Naturally Infected With Toxoplasma Gondii, Kader Yildiz, Oğuz Kul, Sami̇ Gökpinar, Hasan Tarik Atmaca, Yilmaz Emre Gençay, Aycan Nuri̇ye Gazyağci, Cahi̇t Babür, İsmayi̇l Safa Gürcan

Turkish Journal of Veterinary & Animal Sciences

Skeletal muscles (tongue, masseter, leg, intercostal, and diaphragmatic muscles) and brain samples of 100 sheep at slaughter were analysed for the presence of T. gondii tissue cysts along with serum IgG titres. Two methods of isolation by percoll gradient centrifugation and tissue microarray (TMA) technique with immunoperoxidase staining were used. Seropositivity was detected in 88% (88/100) of sheep sera analysed by indirect fluorescent antibody test. Tissue cysts were observed in 46 (52.3%, 46/88) and 15 (17%, 15/88) of the seropositive sheep with the isolation technique and TMA and immunoperoxidase staining, respectively. The diameters of the tissue cysts were 25–58 × …

The Effects Of Supplementing Diets Fed To Pregnant And Lactating Ewes With Saccharomyces Cerevisiae Dried Yeast, Katarzyna Zabek, Stanislaw Milewski, Roman Wójcik, Andrzej Krzysztof Siwicki

Turkish Journal of Veterinary & Animal Sciences

The objective of this study was to determine the effect of supplementing diets fed to ewes (in late pregnancy and during lactation or only during lactation) with Saccharomyces cerevisiae dried brewer’s yeast on the yield and chemical composition of milk and on selected parameters of humoral and cellular immunity. The investigated indicators were analyzed on days 28 and 70 of lactation. The addition of dried yeast contributed to a significant increase in milk yield, and the obtained milk was characterized by a lower somatic cell count, suggesting improved mammary gland health. Diet supplementation had a beneficial influence on the health …

The Influence Of Pomegranate-Peel Extracts On In Vitro Gas Production Kinetics Of Rumen Inoculum Of Sheep, Mohammad Javad Abarghuei, Yousef Rouzbehan, Abdel-Fattah Salem

Turkish Journal of Veterinary & Animal Sciences

Different levels of pomegranate peel extracted by either water (PEH) or solvent mixture (PEM) were evaluated for their effect on in vitro gas production and ruminal fermentation patterns. Two levels of PEH or PEM (15 and 30 mg total phenolics (TP)/g DM of basal diet) were compared to a control (0) level. Ruminal fermentation parameters such as 24-h partitioning factor (PF_{24}), gas yield (GY_{24}), in vitro organic matter disappearance (IVOMD), metabolizable energy (ME), and microbial protein (MP) were calculated. The addition of PEH or PEM had no effect (P > 0.05) on asymptotic gas production, apparent degraded substrate (ADS), IVOMD, ME, …

Polymorphism Of The Ovine Calpastatin Gene In Some Turkish Sheep Breeds, Onur Yilmaz, Tamer Sezenler, Nezi̇h Ata, Yalçin Yaman, İbrahi̇m Cemal, Orhan Karaca

Turkish Journal of Veterinary & Animal Sciences

The calpastatin (CAST) gene has a major effect on muscle growth and meat tenderness after slaughter; it is located on the fifth chromosome in sheep. Blood samples were collected from 720 animals in total from Kıvırcık (KIV), Sakız (SZ), Karacabey Merino (KM), and Gökçeada (GA) sheep populations raised in West Anatolia. The PCR products were digested by the restriction endonuclease MspI. Allele frequencies for M and N alleles of the gene were found to be 0.85 and 0.15 in KIV, 0.80 and 0.20 in KM, 0.99 and 0.01 in GA, and 0.34 and 0.66 in SZ sheep, respectively. It was …

Evaluation Of Ultrasonography As A Diagnostic Tool For Hepatic Hydatid Cysts In Sheep, Hussein Awad Hussein, Mohammed Elrashidy

Turkish Journal of Veterinary & Animal Sciences

This retrospective study was designed to evaluate the use of ultrasonography for the diagnosis of hepatic hydatid cysts. This study was conducted on 22 sheep, which were classified into 2 groups according to their liver health group (healthy liver = 13 and cystic liver = 9). Biochemically, serum concentrations of gamma-glutamyl transferase, aspartate aminotransferase, total bilirubin, and globulins were significantly increased (P < 0.01) while albumin was lowered (P < 0.01) in sheep with cystic livers. Ultrasonographically, rounded, anechoic, and unilocular hydatid cysts with elliptical circumferences ranging from 6 to 10 cm were seen. The interiors of cysts contained echogenic particulate materials, septations, or fine echoes. At the 10th intercostal space (ICS), ventral margin, size, thickness, and angle of livers increased in sheep with hepatic cysts, while the diameter of portal vein decreased in sheep with liver cysts. At the 9th ICS, the circumference of the gall bladder was lowered in sheep with hepatic cysts (P < 0.01). The sensitivity, specificity, and positive and negative predictive values of ultrasonography for diagnosis of hepatic hydatid cysts were 80%, 100%, and 100% and 83%, respectively. In conclusion, ultrasonography alone or in combination with some biochemical parameters reflecting liver function could be helpful for the diagnosis of hepatic hydatid cysts in sheep.

Repeated Superovulation Treatments In Kivircik Ewes During Breeding And Nonbreeding Seasons, Burcu Üstüner, Seli̇m Alçay, Yavuz Nak, Zekari̇ya Nur, Deni̇z Nak, Bi̇lgi̇ner Tuna, Gözde Şi̇mşek, Hakan Sağirkaya

Turkish Journal of Veterinary & Animal Sciences

The present study compared superovulation and embryo recovery rates applied repeatedly during breeding and nonbreeding seasons in the same 20 Kivircik sheep. Trials 1 and 3 were performed during the breeding season and the trial 2 was performed in the nonbreeding season. We synchronized the sheep's estrus cycles and induced a superovulatory response with porcine follicle stimulating hormone (pFSH) injections. Natural mating took place 12 h after the last FSH injection. The embryos were collected by laparotomy 7 days after natural mating. There was no significant difference between the number of corpora lutea (CL) counted following the 1st (breeding) and …

New Understanding Of An Old Phenomenon: Uncontrolled Factors And Misconceptions That Cast A Shadow Over Studies Of The ‘Male Effect’ On Reproduction In Small Ruminants, Trina Jorre De St Jorre, Penny Alison Rhian Hawken, Graeme Bruce Martin

Turkish Journal of Veterinary & Animal Sciences

In female sheep and goats, exposure to males can be used to induce ovulation and thus control and synchronise fertility. This 'male effect' offers simple, cost-effective, and hormone-free management of reproduction in flocks and herds but, despite decades of research, significant gaps in our knowledge impede commercial application. Many of these gaps can be traced back to misconceptions in the early documentation of the male effect, and others are the result of uncontrolled factors in experimental design. Consequently, it is often difficult to draw conclusions from past research, to further our understanding of the phenomenon, and to make recommendations for …

The Effects Of Maternal Diet During Gestation On Postnatal Growth Of Lambs And On Metabolic Hormones In Lambs And Ewes, Amanda R. Fox

Honors Scholar Theses

Fetal programming is defined as when a stimulus at a vital stage of development results in permanent effects that persist throughout the lifetime of the individual (Lucas, 1991). There is evidence that poor maternal diet, consisting of both undernutrition and overnutrition, during gestation has permanent negative effects on the growth and development of offspring through fetal programming. Fetal programming enhances the ability of the fetus to absorb and use nutrients while in-utero, ensuring postpartum survival (Hales and Barker, 1992). However, in a postnatal environment where nutrition is plentiful, fetal programming results in negative health consequences (Wu et al., 2006). We …

Western Australian Farmer Survey 2011, Mandy Curnow, Andrew Van Burgel, Jackie Bucat, Anne Jones

Animal production and livestock published reports

DAFWA commissioned this WA sheep producer survey as an additional data set to the Cooperative Research Centre for Sheep Innovation (Sheep CRC) national producer survey. The data was collected by telephone survey in February and March 2011 for the purposes of gathering benchmark information on a range of sheep management practices. The data collected mostly relates to flock management during the 2010 season. The same survey will be run again in 2014 and a comparison of the information collected will be used to measure how producers have changed their practices during that time.

Producers were phoned and invited to contribute …

Effects Of Poor Maternal Nutrition On Gh, Igf-I, Igfbp-2 And -3, Insulin, And Leptin Concentrations In Pregnant Ewes, Michelle E. Forella

Honors Scholar Theses

Maternal hormones impact fetal growth and development through their effect on nutrient partitioning and placental development. Maternal diet is one factor regulating maternal hormone concentrations. We hypothesized that during gestation, diet alters concentrations of maternal metabolic hormones. Pregnant ewes (n=36; 12/treatment) were individually fed 1 of 3 diets [100% (CON), 140% (OVER), or 60% (RES) NRC requirements] from wk 5 of gestation until parturition. Weekly blood samples (20 mL) were obtained via jugular venipuncture and BW was measured. Concentrations of GH, IGF-I and leptin were determined by RIA. Insulin was determined by ELISA. Data were analyzed using ANOVA. By the …

Live Weight Parameters In Dorper, Damara And Australian Merino Lambs Subjected To Restricted Feeding, Tim Scanlon, Andre M. Almeida, Andrew Van Burgel, Tanya Kilminster, John Milton, Johan C. Greeff, Chris Oldham

Journal articles

Seasonal weight loss (SWL) is a serious constraint to ruminant production in tropical and Mediterranean climates. SWL is controlled using supplementation, costly and difficult to implement in extensive production systems; or alternatively, using breeds with a natural adaptation to tropical climates, namely hair and fat tailed sheep. Albeit a 15-year presence in Australia, little is known on how Dorper and Damara sheep compared to the most widely used sheep breed in Australia, the Australian Merino. In this trial, the responses of the Damara, Dorper and Merino breeds to nutritional stress were compared during a 42-day trial. Seventy-two ram lambs, 24 …

Effect Of Different Transport Temperatures On In Vitro Maturation Of Oocytes Collected From Frozen-Thawed Sheep Ovaries, Özen Banu Özdaş, Alper Baran, Muzaffer Taş, Ümüt Ci̇ri̇t, Kamber Demi̇r, Süleyman Bacinoğlu, Serhat Pabuccuoğlu, Kemal Ak

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the effects of 2 different transport temperatures on the in vitro maturation of oocytes collected from frozen-thawed sheep ovaries. Sheep ovaries were transferred into saline at temperatures of 4 °C and 32 °C. After the 2 experimental groups (A: fresh cortex, B: frozen-thawed cortex) were formed, each group was divided into 2 subgroups (group A1: 4 °C, group A2: 32 °C [control]; group B1: 4 °C, group B2: 32 °C). The cortexes were dissected into slices 1-3 mm thick and pieces of 0.5 cm2. For groups B1 and B2, 1-2 cortex pieces …

The Effect Of Selenized Yeast Supplementation On Some Performance Parameters In Sheep, Bogumila Pilarczyk, Agnieszka Tomza-Marciniak, Zbigniew Dobrzanski, Malgorzata Szewczuk, Tomasz Stankiewicz, Dariusz Gaczarzewicz, Wlodzimierz Lachowski

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the levels of serum selenium in coarse-haired Pomeranian sheep and to evaluate the effect of the oral supplementation of selenized yeast on selected performance parameters in such sheep. The sheep were divided into 2 groups, with 53 ewes forming the experimental group and 34 ewes forming the control group. The concentration of serum selenium in all 87 sheep from the 2 groups was analyzed. The sheep in the experimental group received selenized yeast for the 3 days before and 3 days after mating (1 g preparation/animal daily, with 1 g of preparation …

Presence Of Mycoplasmas In The Respiratory System Of Small Ruminants Managed Under An Extensive Production System, Zinka Maksimovic, Christian De La Fe, Maid Rifatbegovic

Turkish Journal of Veterinary & Animal Sciences

The objective of this study was to investigate the presence of mycoplasmas in the respiratory system of sheep and goat populations managed under extensive production systems. Specific culture and polymerase chain reaction techniques were performed on a total of 88 lung tissue samples and 142 nasal swabs obtained from multiple herds in Bosnia and Herzegovina. A total of 29 samples (12.6%) were found to be positive for mycoplasmas. Mycoplasma ovipneumoniae was detected in the nasal swabs and lungs of affected sheep (n = 19) and in the lungs of asymptomatic goat kids (n = 2), while M. arginini (n = …

Scrapie Cases In The Northern Cyprus, Aydin Gürel, Ahmet Gülçubuk, Nuri̇ Turan, Christopher Richard Helps, Hüseyi̇n Yilmaz

Turkish Journal of Veterinary & Animal Sciences

Scrapie is a fatal neurodegenerative disease affecting small ruminants that causes economic losses in many countries. It is categorized together with other fatal neurodegenerative diseases as a transmissible spongiform encephalopathy. The aim of this study was to investigate clinical cases and the cause of death in a flock of sheep from Northern Cyprus (Turkish Republic of Northern Cyprus) suspected of having scrapie. Brains from 4 sheep showing clinical signs of classical scrapie, out of a flock of 200 animals, were analyzed by histopathological examination and infective prion enzyme-linked immunosorbent assay (ELISA). The 4 affected sheep were 2-3 years of age …

Carbon Farming In Wa, Department Of Agriculture And Food, Western Australia, Department Of Regional Development And Lands, Western Australia

Natural resources published reports

Fact sheets:

1. Applying biochar to improve soil quality and crop productivity

2. Reducing methane emissions from cattle using feed additives

3. Cattle breeding for lower greenhouse gas emissions

4. Managing genetic codes to reduce methane emissions from cattle

5. Managing pastures and stocking rate to reduce methane emissions from cattle

6. Claying as a method of increasing soil carbon content

7. Green and brown manuring as part of carbon farming

8. Permanent environmental plantings to earn carbon credits

9. Nitrous oxide emissions from agricultural soils

10. Sheep genetics in methane reduction

11. Managing sheep pastures to reduce methane production …

Effects Of Grazing Vs. Resting On Soil Chemistry And Plant Community Dynamics For The Central Coast Of California In 2012, Jessica Combs

Animal Science

No abstract provided.

Index Selection In Terminal Sires Improves Early Lamb Growth, G. C. Márquez, W. Haresign, M. H. Davies, G. C. Emmans, Rainer Roehe, L Bünger, G. Simm, Ronald M. Lewis

Department of Animal Science: Faculty Publications

The use of terminal sires (TS) for crossbreeding is integral to the UK sheep industry where approximately 71% of market lambs are sired by TS rams. Early growth of these crossbred lambs affects profitability. The objectives of this study were i) to evaluate the effectiveness of index selection among TS on BW and ADG of their crossbred offspring; and ii) to compare the efficacy of that selection within TS breeds. The most widely used TS breeds in the United Kingdom are Charollais, Suffolk, and Texel. These participated in sire referencing schemes in which they were evaluated on a lean growth …

Sheep: The Simple Guide To Making More Money With Less Work - Cereal-Sheep Zone Of Australia, Bob Hall, Mark Ferguson, Mandy Curnow, Andrew Thompson

Animal production and livestock published reports

The national sheep flock is a mere 40% of the size it was at its peak in the early 1990s. Some would say that it is on the verge of losing its critical mass for survival into the future. Others would see it already as a niche market industry, especially in regards to wool.

Farmers in a recent survey gave two key reasons for either quitting sheep or reducing the flock size: “sheep do not pay” or “the work involved in sheep is unacceptable”. We believe that sheep do pay — and they don’t have to be hard work.

The …

The Effect Of Maternal Undernutrition On Muscle Development In The Ovine Fetus*, Berjan Demi̇rtaş, Mukaddes Özcan

Turkish Journal of Veterinary & Animal Sciences

The effect of maternal undernutrition on muscle development of near-term ovine fetuses was studied in 2 experiments. In each experiment, Welsh mountain ewes were randomly assigned to either control (C) or nutrient-restricted (R) groups. In the first experiment, mild maternal undernutrition (85% of daily nutritional requirement (DNR)), and in the second experiment, severe maternal undernutrition (50% of DNR), were imposed between 0 and 70 days of gestation (dg). Controls for both groups were maintained on 100% of their DNR throughout the experiment. In each experiment, ewes were killed at 126 ± 1 dg by an intravenous injection of pentobarbitone. The …

Prolonged Dystocia, Uterine Necrosis, And Ovariohysterectomy In A Chios Ewe, Christos N. Brozos, Lazaros Lazarides, Isidoros Karagiannis, Evangelos Kiossis, Georgios Tsousis, Vasilios Psychas, Nektarios Giadinis

Turkish Journal of Veterinary & Animal Sciences

A 4-year-old Chios ewe was submitted to the Clinic of Farm Animals (School of Veterinary Medicine, Aristotle University of Thessaloniki). The animal had delivered 2 healthy lambs, 2 days earlier, and was inappetent, depressed, and reluctant to move, while it had tachycardia and tachypnea. The cervix was opened at a dilation of 1 finger in diameter and fetid fetal membranes expelled from the vagina. Serum biochemistry revealed renal and liver disease, while ultrasonography revealed uterine dilatation. Laparotomy revealed a partly autolysed embryo in the uterus and gangrenous metritis. Clostridium perfringens and Escherichia coli were isolated in bacterial cultures from the …

Neuronal And Extraneuronal Lipofuscinosis In Merino Sheep Grazing Asphodelus Aestivus Seeds In Western Turkey, Sümbül Serap Bi̇ri̇nci̇oğlu, Wolfgang Schmahl, Hamdi̇ Avci

Turkish Journal of Veterinary & Animal Sciences

Neuronal and extraneuronal lipofuscinosis was determined in Merino sheep (6 sheep and 3 heads of sheep) of 2-3 years old that had grazed on Asphodelus aestivus seeds. Clinically, the sheep exhibited tremors, paresis, oral and nasal discharge, and severe respiratory distress. Haemorrhagic necrotic tracheitis, aspiration pneumonia, and subacute enteritis were observed in 6 sheep during macroscopic examinations. The brains of the animals were of firm consistency and yellowish brown in colour. Histological examination of the central nervous system revealed that most of the cytoplasm of neurons contained yellowish brown pigment granules. Pigmentations were particularly detected in the pons, medulla oblongata, …

Changing Climate, Changing Business Practices : Lessons From Innovative Growers, David Gray, Nicole Baxter, Meredith Fairbanks, Geraldine Pasqual

Bulletins 4000 -

Climate change and increasing seasonal variability are challenging the production efforts of growers in lower rainfall environments of the Western Australian grain belt. Significantly less rainfall than the long-term average has fallen across southwest Western Australia during the past 30 years.

Seasonal rainfall has also become more variable with later starts to the growing season and a general shift to more summer and less winter rainfall. Day and night-time temperatures, particularly in winter and autumn, have gradually increased, while in some areas, the risk of frost in spring has increased.

Evaluating Glycerin Supplementation On Reproductive Performance Of Sheep, J.A. Walker, G.A. Perry, R. Salverson, P. Nester, C. Schauer, J. Held, K. Olson

Animal Science Faculty Publications

This 3-year study evaluated the effect ofglycerin supplementation on ewe reproductive efficiency,blood glucose and insulin concentrations. Mature Polypayand Rambouillet ewes (n = 225) were orally dosed withglycerin following estrus synchronization at rates of 0, 50,100, 200 or 300 g/hd. In year 3 an additional 16 ewes weresupplemented (SUP) with 0.57 kg of range cake for 21 daysprior to breeding. Blood samples were collected (n = 25ewes) for 10 hours post drenching. Blood was analyzed forinsulin and glucose concentrations. Ewes were exposed torams for 35 days. Pregnancy was determined byultrasonography evaluation. Number of lambs born per eweexposed was not different (P …

Improvement Studies On Mutton Sheep For Marmara Region Conditions: I. Fertility, Lamb Survival, And Growth Traits Of Lambs, Ayhan Ceyhan, Tamer Sezenler, İsmai̇l Erdoğan, Osman Torun

Turkish Journal of Veterinary & Animal Sciences

The present study was conducted to compare the fertility of ewes, the survival rates, and the growth performance of lambs that were crossbred by mating German Black-Headed Mutton (GBM) rams with Kıvırcık ewes. The average lambing rates of the GBM × K(F1), Bandırma-I and Bandırma-II genotype, and Kıvırcık ewes were 80.00%, 75.73%, 76.78%, and 83.72%, respectively. The differences in lambing rates between the genotypes were significant (P < 0.01). The genotypes had a significant effect on litter size at birth, but not on fecundity. The differences in lambs' survival rates between the genotypes were significant (P < 0.05). The birth weight (BW), weaning weight (WW), and yearling live weight (YLW) of the lambs were 3.77 kg, 34.11 kg, and 43.71 kg for the GBM × K (F1); 3.74 kg, 32.98 kg, and 44.91 kg for the Bandırma-I; 3.73 kg, 33.18 kg, and 45.71 kg for the Bandırma-II; and 3.45 kg, 30.92 kg, and 42.22 kg for the Kıvırcık lambs. The results of this study showed that crossbreeding between the GBM and Kıvırcık breed did not significantly increase lamb production. However, crossbred lambs did not have significant survival problems when compared to native Kıvırcık lambs and had higher growth performance than purebred Kıvırcık lambs.

Analysis Of The Orf Region Of The Prion Protein Gene In The Botosani Karakul Sheep Breed From Romania, Steliana Elvira Maria Kevorkian, Mihaela Zaulet, Maria Adina Manea, Sergiu Emil Georgescu, Marieta Costache

Turkish Journal of Veterinary & Animal Sciences

Ovine scrapie is considered a neurodegenerative disease characterized by the accumulation of an abnormal, protease-resistant isoform of PrP. The polymorphisms at codons 136, 154, and 171 are associated with susceptibility to scrapie. In order to detect the existing polymorphisms at the 3 codons we used the real-time PCR technique. The obtained genotypes were confirmed by PCR amplification and sequencing of a 402 bp fragment of exon 3 of the prion gene. Known polymorphisms were detected at codons 136, 154, and 171 and 5 different genotypes were determined. The results showed that all animals were homozygous for A at codon 136 …