Animal Sciences Commons^™

Validation Of Antibodies Used To Study Hypoxia Inducible Factors In Two Species Of Fundulus, Jenna D. Hill

LSU New Orleans Theses and Dissertations

Hypoxia inducible factors (HIFs) are transcription factors and the master regulators of oxygen-dependent gene expression in animals. The focus of this thesis is the distribution of HIF protein in tissues of the fish Fundulus heteroclitus and F. grandis, two widespread species that occur in naturally hypoxic waters. Polyclonal antibodies against HIF-1α, HIF-2α, and HIF-3α were tested on proteins made in vitro and on extracts made from several tissues of normoxic and hypoxic fish. Antibodies against HIF-1α and 3α bound specifically to full length protein made in vitro, and produced bands on western blots of nuclear extracts of near …

Systematic Ichthyofaunal Surveys In Urban And Non-Urban Watersheds, Eugene G. Maurakis, David V. Grimes, Amanda Schutt, Suzy Short

Virginia Journal of Science

Objectives were to model fish species richness relative to natural and anthropogenic variables in Quantico Creek, a forested undisturbed stream environment, and Cameron Run, a highly disturbed urban stream environment in the lower Piedmont-Fall Line region of the Potomac River watershed. Species richness in all stream orders (e.g. avg. range=2.5-9.65 in 1st-3rd orders) of Quantico Creek were significantly higher than those (e.g. avg. range=2.1- 7.6 in 1st -4th orders) of Cameron Run. Fish species richness in Quantico Creek watershed can be modeled by eight factors: season, stream order, elevation, river km, stream width and depth, watershed size, and percent of …

Ecology Of Glacial Relict Fishes In South Dakota's Sandhills Region, Eli Felts

Electronic Theses and Dissertations

Native stream fish zoogeography has changed substantially across North America during the last century as habitat degradation, stream fragmentation and introductions of nonnative species have led to numerous extinctions, extirpations and altered distributions. Insufficient information regarding imperiled species often results in reactive, rather than proactive, management, and knowledge of species status and ecology is critical in identifying conservation priorities. South Dakota populations of three dace species (northern redbelly dace Chrosomus eos, finescale dace Chrosomus neogaeus, and pearl dace Margariscus margarita) are relict of Pleistocene Glaciation and are isolated from the northern core of their distribution, but little information exists regarding …

Predictors Of Regional Establishment Success And Spread Of Introduced Non-Indigenous Vertebrates, Criag R. Allen, Kristine T. Nemec, Donald A. Wardwell, Justin D. Hoffman, Mathew L. Brust, Karie L. Decker, Daniel Fogell, Jennifer Hogue, Aaron Lotz, Thaddeus Miller, Marcy Pummill, Luis E. Ramirez-Yañez, Daniel R. Uden

Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications

Aim To provide the first analysis of predictors of both establishment and spread, both within and across taxa, for all vertebrate taxa within a region.We used Florida, USA, as our study system because it has a well-documented history of introduction and invasion, and is a hotspot for biological invasions.

Location Florida, USA.

Methods We analysed non-indigenous species (NIS) data from peninsular Florida – which included both successful and unsuccessful introductions from all vertebrate classes – to determine the best predictors of both establishment and spread for fish (65 species), herpetofauna (63 species), birds (71 species) and mammals (25 species). We …

Metazoan Parasites Of Fish Species From Lake Sığırcı (Edirne, Turkey), Hafi̇ze Si̇bel Çolak

Turkish Journal of Veterinary & Animal Sciences

A total of 366 specimens of 10 fish species inhabiting Lake Sığırcı were examined between April 2009 and February 2010. Inside or outside of these fish, 33 parasite species were identified. From these parasites, 18 monogenean, 5 digenean trematode, 3 cestode, 3 acanthocephalan, 3 nematode, and 1 Mollusca: Bivalvia (glochidia) species were recorded. Diplostomum spp., Tylodelphys clavata, glochidia-larvae, and monogenean species were found to be common parasites in all the fish species examined. The highest prevalence and mean infection intensity with Diplostomum spp. were found in Lepomis gibbosus (prevalence 91.6%, mean intensity 5.6 individuals per fish) and in Scardinius erythrophthalmus …

Effects Of Pristane On Growth Of Pink Salmon (Oncorhynchus Gorbuscha), Brielle D. Kemis, Bonita Nelson

STAR Program Research Presentations

Pristane is a naturally occurring hydrocarbon that is generated by copepods in marine ecosystems. Organisms that eat copepods do not readily metabolize pristane, which causes it to accumulate throughout the marine food web. Understanding how pristane affects marine fish is essential because pristane is often used as a bioindicator in lab studies and may be influencing results in those studies. Also, this hydrocarbon may significantly reduce growth of marine fish in the wild. A previous study indicated that pristane is an appetite suppressant and growth retardant in fish. However, the study implemented a feeding regime with unnaturally high levels of …

Clinical Anesthesia And Analgesia In Fish, Lynne U. Sneddon

Veterinary Science and Medicine Collection

Fish have become a popular experimental model and companion animal, and are also farmed and caught for food. Thus, surgical and invasive procedures in this animal group are common, and this review will focus on the anesthesia and analgesia of fish. A variety of anesthetic agents are commonly applied to fish via immersion. Correct dosing can result in effective anesthesia for acute procedures as well as loss of consciousness for surgical interventions. Dose and anesthetic agent vary between species of fish and are further confounded by a variety of physiological parameters (e.g., body weight, physiological stress) as well as environmental …

The Effect Of Bleeding On Peripheral Blood And Head Kidney Hematopoietic Tissue In Common Carp (Cyprinus Carpio), Elzbieta Kondera, Aneta Dmowska, Monika Rosa, Malgorzata Witeska

Turkish Journal of Veterinary & Animal Sciences

Adverse environmental conditions (contamination of water with nitrite, pesticides, or other pollutants), nutritional deficiencies, bacterial and viral infections, and parasitic invasions may induce anemia in fish, in both natural and aquaculture conditions. Changes in hematological values and cellular composition of peripheral blood and head kidney hematopoietic tissue of bled common carp (Cyprinus carpio L.) were evaluated over a 4 week period post-bleeding (30% blood loss). The results showed that fish very quickly compensated for anemia. Erythrocyte count (RBC), hemoglobin concentration (Hb), and hematocrit (Ht) gradually increased to values significantly higher than they were before bleeding; this was accompanied by an …

Weight-Length Relationships Of 28 Fish Species In The Sea Of Marmara, Nazli Demi̇rel, Eli̇f Murat Dalkara

Turkish Journal of Zoology

Weight-length relationships (WLRs) for 28 fish species were investigated in an enclosed basin in the Sea of Marmara. Due to low sample sizes ( 0.05). In this study, WLRs for Pomatoschistus marmoratus are presented for the first time in Turkish waters. Additionally, this study represents the first results for the entire Sea of Marmara and carries significant importance for the WLR database in light of previous studies, which only provided information for limited parts of this sea.

The Efficacy Of Three Types Of Analgesic Drugs In Reducing Pain In The Rainbow Trout, Oncorhynchus Mykiss, Jessica J. Mettam, Lois J. Oulton, Catherine R. Mccrohan, Lynne U. Sneddon

Veterinary Science and Medicine Collection

Recent research has shown the possibility of pain perception in fish; therefore, the use of analgesia or “painkillers” should be considered for invasive procedures. However, there is relatively little information on the effectiveness of analgesic drugs nor on the appropriate dose for fish. This study assessed the efficacy of three types of drug: an opioid, buprenorphine, a non-steroidal anti-inflammatory drug (NSAID), carprofen and a local anaesthetic, lidocaine. Each drug was tested at three doses on rainbow trout that were noxiously stimulated and the most effective dose was also given to fish experiencing no pain to investigate side-effects. Ventilation rate and …

Implications Of Community Concordance For Assessing Stream Integrity At Three Nested Spatial Scales In Minnesota, U.S.A., Christine L. Dolph, David D. Huff, Christopher J. Chizinski, Bruce Vondracek

Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications

1. Fish and invertebrate assemblage data collected from 670 stream sites in Minnesota (U.S.A.) were used to calculate concordance across three nested spatial scales (statewide, ecoregion and catchment). Predictive taxa richness models, calibrated using the same data, were used to evaluate whether concordant communities exhibited similar trends in human-induced taxa loss across all three scales. Finally, we evaluated the strength of the relationship between selected environmental variables and the composition of both assemblages at all three spatial scales.

2. Significant concordance between fish and invertebrate communities occurred at the statewide scale as well as in six of seven ecoregions and …

Simulation Of Spatial Movement That Potentially Maximizes Assessment, Presence, And Defence In Territorial And Home-Ranging Animals, With Special Reference To Territorial Sex-Changing Fishes, Thomas R. Brown, Joshua Jowers, Marvin M.F. Lutnesky

Biology Faculty Publications (Archived)

Question: Are there territory sizes, shapes, and movement rules that animals can use to maximize their presence for territorial and reproductive purposes? Features of the model: Individual-oriented simulation model of a focal male, a female (his mate), and six other males in adjacent territories. Simulation variables were territory size and aspect (length-to-width ratio), velocity, step size, and potential movement angle. Each simulation represented 10,000 s of real time, and simulations were repeated until convergence on maximum mean encounter rates (MER) of a focal male with the female and other males was reached. Ranges of variables: Simulations consisted of three velocities …

Assessment Of Heavy Metal Concentrations In Water, Plankton, And Fish Of Lake Manzala, Egypt, Mohamed Bahnasawy, Abdel Aziz Khidr, Nadia Dheina

Turkish Journal of Zoology

The levels of some heavy metals (Cu, Zn, Cd, and Pb) were determined in water, plankton, and fish (Liza aurata) collected from 5 sites in Lake Manzala. Metals in the water and fish exhibited a significant seasonal and regional variation, in which all metals attained their maximum value during summer, while the lowest levels were found during winter. The concentration of different metals in water, plankton, and fish tissues followed the same order: Zn > Cu > Pb > Cd. The mean concentrations of metals in the water were as follow: Cu, 0.055; Zn, 0.311; Cd, 0.020; and Pb, 0.022 mg/L. The Cd …

Disodium Edta Used As Anticoagulant Causes Hemolysis In Common Carp Blood, Malgorzata Witeska, Wioleta Wargocka

Turkish Journal of Veterinary & Animal Sciences

Disodium EDTA used as anticoagulant for common carp blood caused a significant increase and high variability in hematocrit readings comparing to the heparinized samples. Na2EDTA induced erythrocyte swelling, causing cell membrane disruption (hemolysis). The nuclei released from destroyed cells changed shape from oval to round, and underwent gradual swelling and vacuolation, followed by karyolysis. The degree of observed changes was similar at all Na2EDTA concentrations (0.1, 0.5, and 1.0 mg/mL); on the other hand, percentage of abnormal and destroyed cells showed considerable differences among blood sampled from various carp individuals. The obtained results revealed that Na2EDTA caused gradual damage to …

Comparisons Of Fish Species Inside And Outside Of Marine Protected Areas Off The South Central Coast Of California, David E. Rasmussen

Master's Theses

COMPARISONS OF FISH SPECIES INSIDE AND OUTSIDE OF MARINE PROTECTED AREAS OFF THE SOUTH CENTRAL COAST OF CALIFORNIA

David E. Rasmussen

From 2004-2008 and 2008-2009 Cal Poly University researchers conducted two separate tag and recapture studies of nearshore fish populations along California’s central coast. Three locations were sampled that have experienced different degrees of fishing pressure. Big Creek MPA has been closed to all fishing from 1993 onwards and is the farthest from port of all locations. Cambria lies closest to port of the three locations and because of this has the highest fishing pressure. Piedras Blancas lies midway between …

Aquatic Fauna - Biological Survey Ten Mile Brook Dam Margaret River - Fisheries Research Contract Report No. 22, 2010, Craig Lawrence, C Hugh, Renae Larsen, J Ledger, Phil Vercoe

Fisheries Research Contract Reports

Ten Mile Brook Dam has a surface area of 275 Ha and receives water from a 10 km2 catchment (Figure 1). It has a total storage capacity of 1,691 ML. At the time of this study the dam was 89 % capacity (1,511 ML), however as recently as June 2009 water storage was as low as 663 ML (39 % capacity).

In order to improve the drinking water supply to the town of Margaret River, the Water Corporation is investigating the option of pumping bore water into Ten Mile Brook Dam. This would remove the need to transfer water from …

About Fish, Humane Society Institute For Science And Policy

Aquaculture Collection

Although a number of fish species have been studied in their freshwater life stages, further research on the behavior and habitat requirements of ocean-going fish is required. While fishes have historically been regarded as more “primitive” than other vertebrate groups, Rodriguez et al concluded that several memory and learning systems of bony fishes are noticeably similar to those of reptiles, birds, and mammals. When animals as small and under appreciated as fish display complex mating systems, parental care, and demonstrate the ability to traverse significant distances using olfactory and celestial cues, it is clear that previously established definitions of intelligence …

The Index Of Biological Integrity And The Bootstrap: Can Random Sampling Error Affect Stream Impairment Decisions?, Christine L. Dolph, Aleksey Y. Sheshukov, Christopher J. Chizinski, Bruce Vondracek, Bruce N. Wilson

Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications

Multimetric indices, such as the Index of Biological Integrity (IBI), are increasingly used bymanagement agencies to determine whether surface water quality is impaired. However, important questions about the variability of these indices have not been thoroughly addressed in the scientific literature. In this study, we used a bootstrap approach to quantify variability associated with fish IBIs developed for streams in two Minnesota river basins. We further placed this variability into a management context by comparing it to impairment thresholds currently used in water quality determinations for Minnesota streams. We found that 95% confidence intervals ranged as high as 40 points …

Environmental And Endogenous Factors Influencing Emigration In Juvenile Anadromous Alewives, Benjamin I. Gahagan, Eric T. Schultz, Katie E. Gherard

EEB Articles

We analyzed juvenile anadromous alewife migration at Bride Lake, a coastal lake in Connecticut, during summer 2006 and found that migration on 24-hour and seasonal timescales was influenced by conditions of the environment and characteristics of the individual. To identify environmental cues of juvenile migration, we continuously video recorded fish at the lake outflow and employed information-theoretic model selection to identify the best predictors of daily migration rate. More than 80% of the approximately 320,000 juveniles that migrated from mid-June to mid-August departed in three pulses lasting one or two days. Pulses of migration were associated with precipitation events, transient …

Absence Of Predation Eliminates Coexistence: Experience From The Fish-Zooplankton Interface, Z. M. Gilwicz, Wayne A. Wurtsbaugh, E. Szymansk

Watershed Sciences Faculty Publications

Examples from fishless aquatic habitats show that competition among zooplankton for resources instigates rapid exclusion of competitively inferior species in the absence of fish predation, and leads to resource monopolization by the superior competitor. This may be a single species or a few clones with large body size: a cladoceran such as Daphnia pulicaria, or a branchiopod such as Artemia franciscana, each building its population to a density far higher than those found in habitats with fish. The example of zooplankton from two different fish-free habitats demonstrates the overpowering force of fish predation by highlighting the consequences of its absence. …

The Effects Of Ichthyophthiriasis On Some Haematological Parameters In Common Carp, Malgorzata Witeska, Elzbieta Kondera, Katarzyna Lugowska

Turkish Journal of Veterinary & Animal Sciences

The present study examined the haematological effects of ichthyophthiriasis in common carp. Blood was collected from 4 groups of juvenile carp: 1. healthy; 2. with minor symptoms of ichthyophthiriasis; 3. with severe symptoms of ichthyophthiriasis (moribund); survivors of ichthyophthiriasis after a 3-week recovery period. Blood was subjected to a standard haematological procedure and the following parameters were evaluated: haematocrit (Ht), haemoglobin concentration (Hb), erythrocyte (RBC) and leukocyte (WBC) counts, mean cell volume (MCV), mean cell haemoglobin (MCH), and mean cell haemoglobin concentration (MCHC). The differential leukocyte count and thrombocyte count were calculated using stained blood smears. No change in Ht …

Diel Fish Habitat Selection In A Tributary Stream, Andria K. Salas, Eric B. Snyder

Peer Reviewed Publications

This study investigated the location and diel habitat preferences (at 100 m reach scale) of fish in a small tributary stream in late spring, early summer. During the day, coho (Oncorhynchus kisutch) preferred areas with more cover (deeper, greater extent of undercut banks) vs. night when LWD was preferred (Pearson correlation and step-wise MLR). Chinook (O. tshawytscha) exhibited an opposite pattern, preferring LWD during the day vs. higher velocity at night. This suggests these two potadromous species may be partitioning resources. Pooling coho, chinook and rainbow trout (O. mykiss) indicated reaches with more LWD …

Investigation Of Van Gogh-Like 2 Mrna Regulation And Localisation In Response To Nociception In The Brain Of Adult Common Carp (Cyprinus Carpio), Siobhan C. Reilly, Anja Kipar, David J. Hughes, John P. Quinn, Andrew R. Cossins, Lynne U. Sneddon

Veterinary Science and Medicine Collection

The Van Gogh-like 2 (vangl2) gene is typically associated with planar cell polarity pathways, which is essential for correct orientation of epithelial cells during development. The encoded protein of this gene is a transmembrane protein and is highly conserved through evolution. Van Gogh-like 2 was selected for further study on the basis of consistent regulation after a nociceptive stimulus in adult common carp and rainbow trout in a microarray study. An in situ hybridisation was conducted in the brain of mature common carp (Cyprinus carpio), 1.5 and 3 h after a nociceptive stimulus comprising of an acetic acid injection to …

Fisheries Occasional Publication No.70 - Stakeholder Survey 2008, Tara Baharthah

Fisheries Occasional Publications

A telephone survey of Department of Fisheries’ stakeholders was conducted in August 2008 in order to evaluate their perception of the Department’s management of commercial and recreational fishing, pearling and aquaculture, and fish and fish habitat protection. The survey also assessed satisfaction with the level of service provided to stakeholders and their involvement in decision making processes. The results of this survey were compared to similar surveys conducted during 2002, 2004, 2006 and a survey of the general public conducted during 2008. The sample comprised of 149 interviews – 39 representing the commercial fishing sector, 41 representing the recreational fishing …

Slides: Market-Based Stream Flow Restoration And Mitigation, Amanda Cronin

Western Water Law, Policy and Management: Ripples, Currents, and New Channels for Inquiry (Martz Summer Conference, June 3-5)

Presenter: Amanda Cronin, Washington Water Trust, Seattle, WA

23 slides

Agenda: Western Water Law, Policy And Management: Ripples, Currents, And New Channels For Inquiry, University Of Colorado Boulder. Natural Resources Law Center, Western Water Policy Program

Western Water Law, Policy and Management: Ripples, Currents, and New Channels for Inquiry (Martz Summer Conference, June 3-5)

In many pockets of the American West, stresses and demands on water resources are overwhelming our capacity to effectively manage change and accommodate the diversity of interests and values associated with our limited water resources.

This event will offer an opportunity for lawyers, policymakers, and water professionals to engage the experts on the challenges and emerging solutions to the most pressing water policy and management issues of the day.

Slides: Finding Flows: Fish Still Need Water Everyday, Melinda Kassen

Western Water Law, Policy and Management: Ripples, Currents, and New Channels for Inquiry (Martz Summer Conference, June 3-5)

Presenter: Melinda Kassen, Director of the Western Water Project, Trout Unlimited

12 slides

Temporal Shifts In Demography And Life History Of An Anadromous Alewife Population In Connecticut, Justin P. Davis, Eric T. Schultz

EEB Articles

Populations of anadromous alewives (Alosa pseudoharengus) are declining throughout much of their range, particularly in southern New England where fishery moratoriums have recently been instituted in three states. The alewife run at Bride Brook, a coastal stream in East Lyme, Connecticut, was studied from 2003-06 to assess shifts in demography and life history. Annual censuses of abundance, along with sampling for size, age, and spawning history structure were conducted. These data were compared to similar data in 1966-67 at this site. Recent alewife runs at Bride Brook featured lower abundance and younger, smaller fish that were less likely to be …

Fisheries Occasional Publication No. 63 - Western Rock Lobster Ecological Risk Assessment, Department Of Fisheries, Western Australia, International Risk Consultants Group Of Companies

Fisheries Occasional Publications

This Ecological Risk Assessment, conducted by IRC Environment (part of International Risk Consultants) and Department of Fisheries WA (FWA) was the result of an initiative by the WA Fishing Industry Council (WAFIC) and (FWA). This report is based on a risk assessment workshop undertaken in February 2001 at the FWA Marine Research Laboratories in Waterman but includes additional background material to justify some of the rankings of the workshop.

The broad intent of the workshop was to provide a register of the potential main ecological risks that arise from the various activities carried out by the western rock lobster (WRL) …

Evaluating The Effectiveness Of Grassbed Treatments As Habitat For Juvenile Black Bass In A Drawdown Reservoir, D. R. Ratcliff, Wayne A. Wurtsbaugh, J. Zustak

Watershed Sciences Faculty Publications

Many reservoirs in arid regions experience highly variable water levels caused by seasonal inflow fluctuations and designated outflow requirements. At Shasta Lake, California, managers plant cereal-grain grassbeds on exposed drawdown shorelines to increase juvenile fish habitat, localize productivity, and increase invertebrate fish prey. To determine the efficacy of these plantings, the abundance of juvenile black basses Micropterus spp. (20–55 mm standard length) and the amount of periphyton and macroinvertebrate prey were compared among three treatment types: (1) planted grassbeds of cereal barley Hordeum vulgare; (2) artificial rope grassbeds, which eliminated physical deterioration and nutrient release; and (3) nonplanted control sites …