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Articles 121 - 150 of 192

Full-Text Articles in Animal Sciences

Heritable Melanism And Parasitic Infection Both Result In Black-Spotted Mosquitofish, Lisa Horth, David Gauthier, Wolfgang Vogelbein Jan 2013

Heritable Melanism And Parasitic Infection Both Result In Black-Spotted Mosquitofish, Lisa Horth, David Gauthier, Wolfgang Vogelbein

Biological Sciences Faculty Publications

Male Gamhusia holhrooki (Eastern Mosquitofish) express a heritable pigmentation polymorphism approximate to 99% of males are silver, and only approximate to 1% have a melanic, black-spotted pattern. Sex-linkage, an autosomal modifier, and temperature control the expression of this heritable melanism. In many teleosts, melanin also accumulates around the site of parasitic invasion. We have identified black-spot disease in wild mosquitofish from their native habitat. Here, we demonstrate convergence upon the black-pigmented phenotype through two means: 1) heritable melanism, and 2) melanic spotting on the silver genotype that results from infection with immature encysted trematodes. Females are silver and express greater …


Timing Of Castration, With Or Without Analgesia, On Growth Performance, Hematology, And Behavior Of Beef Calves, April C. Brown Dec 2012

Timing Of Castration, With Or Without Analgesia, On Growth Performance, Hematology, And Behavior Of Beef Calves, April C. Brown

Graduate Theses and Dissertations

Angus-sired (n = 30) and Hereford-sired (n = 32) bull calves were assigned randomly to 1 of 4 treatments. Treatments were 1) surgical castration near birth, 2) oral administration of the analgesic meloxicam (1 mg/kg of body weight) followed by surgical castration near birth, 3) surgical castration at weaning, or 4) surgical castration at weaning with oral administration of the analgesic meloxicam (1 mg/kg of body weight). Data was recorded for behavior, hematology, and growth at birth and weaning. Calf standing and lying activity was monitored at both stages by recording x and y axis positions of a data logger …


Chronic Ethanol Exposure Causes Disinhibited Behavior In Drosophila, Estrella Padilla, Kyung-An Han Jul 2012

Chronic Ethanol Exposure Causes Disinhibited Behavior In Drosophila, Estrella Padilla, Kyung-An Han

COURI Symposium Abstracts, Summer 2012

Ethanol consumption is frequently associated with disinhibited sexual and locomotor behaviors in humans. Dopamine, a major monoamine found in vertebrates and invertebrates, is implicated in these processes since it regulates motivation, reward, motor control, and sexual behaviors. The goal of this study is to elucidate the role of dopamine in ethanol-associated behavioral disinhibition using the genetically tractable model system Drosophila melanogaster. Previous studies have shown a requirement for normal dopamine neurotransmission for ethanol-induced courtship disinhibition. Furthermore, genetic manipulation of the dopamine system revealed that D1 receptor activity is important for disinhibition and D2 for sensitization. The current study focuses …


The Effects Of Dependent Infants On The Social Behavior Of Mantled Howler Monkeys (Alouatta Palliate), Keziah Katz Apr 2012

The Effects Of Dependent Infants On The Social Behavior Of Mantled Howler Monkeys (Alouatta Palliate), Keziah Katz

Honors Theses and Capstones

Six weeks of observation on eight groups of wild mantled howler monkeys revealed that the presence of a dependent infant alters the frequency of social interactions between a female with an infant and other adult monkeys. Males preferred to spend time with females without infants but still spent more time with females with infants than with males. Females without infants spent significantly less time with females with infants than with males or other females without infants and females with infants decreased their frequency of social interaction overall without preference for males, females or other females with infants.


Observations Of Oviposition Behavior Among North American Tiger Beetle (Coleoptera: Carabidae: Cicindelinae) Species And Notes On Mass Rearing, Mathew L. Brust, C. Barry Knisley, Stephen M. Spomer, Kentaro Miwa Jan 2012

Observations Of Oviposition Behavior Among North American Tiger Beetle (Coleoptera: Carabidae: Cicindelinae) Species And Notes On Mass Rearing, Mathew L. Brust, C. Barry Knisley, Stephen M. Spomer, Kentaro Miwa

University of Nebraska State Museum: Entomology Papers

Although the larvae of a large number of North American tiger beetle species have been described, little information exists on the oviposition behavior of female tiger beetles at the species level. In this study, we found that the oviposition behavior varied widely among species, with some species utilizing more than one method. We found that, contrary to many references, several tiger beetle species did not oviposit from above the soil surface by inserting the ovipositor into the soil. Instead, several species oviposited by tunneling over 20 mm below the soil surface. In addition, we outline recently used successful protocols for …


Ecology And Behaviour Of The Black-Billed Capercaillie (Tetrao Urogalloides Stegmanni) In The Khentej Mountains, Mongolia, Siegfried Klaus, Karl-Heniz Schindlatz, Alexander V. Andreev, Hans-Heiner Bergmann Jan 2012

Ecology And Behaviour Of The Black-Billed Capercaillie (Tetrao Urogalloides Stegmanni) In The Khentej Mountains, Mongolia, Siegfried Klaus, Karl-Heniz Schindlatz, Alexander V. Andreev, Hans-Heiner Bergmann

Erforschung biologischer Ressourcen der Mongolei / Exploration into the Biological Resources of Mongolia, ISSN 0440-1298

Introduction

A common inhabitant of East Siberian larch forests, the Siberian or black-billed capercaillie (Tetrao urogalloides) ranges south to the limits of the boreal forests in the northern Mongolian mountains and east to the very coast of the Asian continent (KLAUS et al. 1989). Tetrao urogalloides MIDDENDORF (1851) has priority over T. parvirostris BONAPARTE (1856). Therefore, we prefer the T. urogalloides.

The subspecies T.u. stegmanni was first described on the basis of morphological differences by POTAPOV (1985) using specimens collected during Russian expeditions by KOZLOVA (1930). This description was based on 18 males in the collection at …


Effect Of Predator Type, Season, Brood Size, And West Nile Virus Infection On The Nest Defense Behavior Of Male And Female Eastern Bluebirds, Kayde Lynn Gilbert Jan 2012

Effect Of Predator Type, Season, Brood Size, And West Nile Virus Infection On The Nest Defense Behavior Of Male And Female Eastern Bluebirds, Kayde Lynn Gilbert

Online Theses and Dissertations

The intensity of nest defense by birds can be influenced by many factors, including a parent's sex, brood size, stage of breeding season, type of predator, and physical condition. Because previous studies have produced conflicting results concerning the effects of these factors on the nest defense behavior of birds, additional studies are needed to better clarify how and why such factors influence behavior. No one to date has examined the possible effect of a viral infection on avian nest defense behavior. Thus, my objectives were to determine the effect of adult sex, brood size, stage of the breeding season, predator …


Seasonal And Group Effects On Dairy Cow Behavior In Large Yards, Akbar Nikkhah, Rasool Kowsar Jan 2012

Seasonal And Group Effects On Dairy Cow Behavior In Large Yards, Akbar Nikkhah, Rasool Kowsar

Turkish Journal of Veterinary & Animal Sciences

In mechanized modern dairy facilities with competitive environments, monitoring behavior provides opportunities to manipulate and optimize the nutritional, health, and social status of high-merit cows. The objective of the current study was to determine seasonal and cow group effects on the eating, ruminating, standing, and lying behaviors of dairy cows in large yards. Seasonal data on various behaviors of lactating cows in different production and lactation stages were collected continuously for 26 months, from December 2006 through February 2008. The herd had approximately 3000 dairy cattle housed in groups within specific yards. A total of 415 multiparous high-producing cows (MH), …


Chimpanzee (Pan Troglodytes) Responses To Visitors Using Chimpanzee-Friendly Behaviors, Daniella Bismanovsky Jan 2012

Chimpanzee (Pan Troglodytes) Responses To Visitors Using Chimpanzee-Friendly Behaviors, Daniella Bismanovsky

All Master's Theses

Many studies suggest that zoo visitors are a cause of stress among animals; among primates, visitor presence can lead to an increase in aggressive displays, time spent non-visible to the public, and a decrease in overall activity. This study tested the effectiveness of using species-specific behaviors among a group of captive chimpanzees. There were 2 conditions: a control, and an experimental condition in which visitors were asked to adopt a stooped posture or lean on the railing, and show a chimpanzee play face. The visitors stooped their posture, sat, and leaned on the railing significantly more in the experimental condition …


The Evolution Of Lateralized Foot Use In Parrots: A Phylogenetic Approach, Culum Brown, Maria Magat Nov 2011

The Evolution Of Lateralized Foot Use In Parrots: A Phylogenetic Approach, Culum Brown, Maria Magat

Sentience Collection

Cerebral lateralization refers to the division of cognitive function in either brain hemisphere and may be overtly expressed as behavioral asymmetries, such as handedness. The evolutionary history of laterality is of considerable interest due to its close link with the development of human language. Although considerable research effort has aimed at the proximate explanations of cerebral lateralization, considerably less attention has been paid to ultimate explanations. The extent to which laterality is constrained by phylogeny or shaped by ecological forces through natural selection has received little attention. Here, the foot preference of 23 species of Australian parrots was examined to …


The Complexities Of Wolf Spider Communication: Exploring Courtship Signal Function In Rabidosa Rabida, Dustin J. Wilgers Jul 2011

The Complexities Of Wolf Spider Communication: Exploring Courtship Signal Function In Rabidosa Rabida, Dustin J. Wilgers

School of Biological Sciences: Dissertations, Theses, and Student Research

Evidence of signal complexity is seemingly pervasive across animal communication systems. Exploring signal function may provide insight into how these displays evolved and are maintained. This dissertation examines the courtship signal function in a grassland wolf spider. Rabidosa rabida lives in an extremely complex environment, and males use complex displays incorporating both visual and seismic modalities. Using several approaches I provide insight into the content and efficacy of the various signal components, as well as how variation in these displays influence female mating decisions in isolation and combined.

First, I manipulated male and female body condition using diet quantity manipulations …


Vocalization Behavior Of The Endangered Bahama Oriole (Icterus Northropi): Ontogenetic, Sexual, Temporal, Duetting Pair, And Geographic Variation, Valerie A. Lee Mar 2011

Vocalization Behavior Of The Endangered Bahama Oriole (Icterus Northropi): Ontogenetic, Sexual, Temporal, Duetting Pair, And Geographic Variation, Valerie A. Lee

Faculty Works

Many birds communicate via a diverse set of vocalizations, but the contexts, roles, and structure of their varied songs and calls may change with age, differ between sexes, and vary temporally and geographically. In New World orioles, most tropical species exhibit the ancestral states of sexual monochromatism (both sexes have similar plumage) and monovocalism (both sexes sing and often duet together), whereas migratory temperate species tend toward dichromatism (males brightly colored and females drab) and divocalism (males sing almost exclusively). In this study, I examined the vocalizations of the Bahama Oriole, a non-migratory, monochromatic species, to learn where it fits …


Gray Wolf (Canis Lupus) Movements And Behavior Around A Kill Site And Implications For Gps Collar Studies, L. David Mech Jan 2011

Gray Wolf (Canis Lupus) Movements And Behavior Around A Kill Site And Implications For Gps Collar Studies, L. David Mech

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Global Positioning System (GPS) radio-collars are increasingly used to estimate Gray Wolf (Canis lupus) kill rates. In interpreting results from this technology, researchers make various assumptions about wolf behavior around kills, yet no detailed description of this behavior has been published. This article describes the behavior of six wolves in an area of constant daylight during 30 hours, from when the pack killed a Muskox (Ovibos moschatus) calf and yearling on Ellesmere Island, Nunavut, Canada, to when they abandoned the kill remains. Although this is only a single incident, it demonstrates one possible scenario of pack …


Use Of Modified Cages Attached To Growing Calves To Measure The Effect Of Stable Flies On Dry Matter Intake And Digestibility, And Defensive Movements, L. A. Schole, D. B. Taylor, D. R. Brink, K. J. Hanford Jan 2011

Use Of Modified Cages Attached To Growing Calves To Measure The Effect Of Stable Flies On Dry Matter Intake And Digestibility, And Defensive Movements, L. A. Schole, D. B. Taylor, D. R. Brink, K. J. Hanford

Department of Animal Science: Faculty Publications

The effects of stable flies on growing calves were examined using fly cages attached to the animals. Dry matter intake, DM digestibility (DMD), and behavioral responses of calves were monitored. Nine Holstein calves were exposed to 3 levels of stable flies (0, 10, 100 flies/animal) 3 times daily for 30 min. The study consisted of a 4-period crossover design; each period included 5-d adaptation, 7-d exposure, and 5-d postexposure. Calves were weighed at the beginning and end of each period. Feed consumption was continuously recorded. Fecal samples taken during and after exposure were used to determine DMD. Three calves were …


Aggressive Body Language Of Bears And Wildlife Viewing: A Response To Geist (2011), Stephen F. Stringham Jan 2011

Aggressive Body Language Of Bears And Wildlife Viewing: A Response To Geist (2011), Stephen F. Stringham

Human–Wildlife Interactions

No abstract provided.


A Comparison Of Prairie Vole Audible And Ultrasonic Pup Calls And Attraction To Them By Adults Of Each Sex, Thomas A. Terleph Jan 2011

A Comparison Of Prairie Vole Audible And Ultrasonic Pup Calls And Attraction To Them By Adults Of Each Sex, Thomas A. Terleph

Biology Faculty Publications

Rodent pups of many species emit both ultrasonic vocalizations (USVs) and calls spanning into a lower frequency range, audible to humans (AUDs), yet there has been little systematic comparison of these different call types, or analyses of how they might differ in signal function. Here the spectral and temporal characteristics of USV and AUD pup calls are described for the prairie vole (Microtus ochrogaster), a model used in studies of monogamous mating and biparental care, and a species with an unusually large functional and anatomical representation of auditory cortex. Findings provide a detailed description of each call type, …


Does The Manipulation Of Tail Position Facilitate Extinction Of Canine Phobic Behavior?, Rebecca A. Zarate May 2010

Does The Manipulation Of Tail Position Facilitate Extinction Of Canine Phobic Behavior?, Rebecca A. Zarate

Theses and Dissertations - UTB/UTPA

The phobic behavior of interest is tail tucking which is indicative of submission. In this study the tail was physically manipulated by the use of a custom made harness to hold the tail up in a dominant position to ideally extinguish phobic behavior through reciprocal inhibition. The harness did not hold the tail up the entire duration of the interventions, although it still held the base of the tail up. The intervention did not appear to have an affect on the two of the five subjects. The three more phobic canines did display some behavioral changes, although the changes noticed …


Identifying And Understanding The Spatial Distribution Of Bobcat And Coyote Behavior, Ryan Radford Wilson May 2010

Identifying And Understanding The Spatial Distribution Of Bobcat And Coyote Behavior, Ryan Radford Wilson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A common observation in animal space use studies is that animals do not use space uniformly, but rather use some areas of their home ranges and territories with much higher intensity than others. Numerous methods have been developed to estimate these "core areas"; however, all of the current methods available are based on arbitrary rules. Additionally, most studies do not attempt to understand what behavioral processes lead to the observed patterns of non-uniform space use. This study has four main objectives: 1) to develop an objective and more precise method for estimating core areas, 2) to understand the processes leading …


A Survey Of The Management And Development Of Captive African Elephant (Loxodonta Africana) Calves: Birth To Three Months Of Age, Nicole L. Kowalski, Robert H.I. Dale, Christa L. H. Mazur Mar 2010

A Survey Of The Management And Development Of Captive African Elephant (Loxodonta Africana) Calves: Birth To Three Months Of Age, Nicole L. Kowalski, Robert H.I. Dale, Christa L. H. Mazur

Scholarship and Professional Work - LAS

We used four surveys to collect information about the birth, physical growth, and behavioral development of 12 African elephant calves born in captivity. The management of the birth process and neonatal care involved a variety of standard procedures. All of the calves were born at night, between 7PM and 7AM. The calves showed a systematic progression in behavioral and physical development, attaining developmental milestones at least a quickly as calves in situ. This study emphasized birth-related events, changes in the ways that calves used their trunks, first instances of behaviors, and interactions of the calves with other, usually adult, elephants. …


Growth And Age At Reproductive Maturity Of The Carolina Pigmy Rattlesnake, Sistrurus M. Miliarius (Reptilia: Serpentes), Kevin Robert Messenger Jan 2010

Growth And Age At Reproductive Maturity Of The Carolina Pigmy Rattlesnake, Sistrurus M. Miliarius (Reptilia: Serpentes), Kevin Robert Messenger

Theses, Dissertations and Capstones

Growth and age at reproductive maturity are life history attributes that play an important role in the development of proper management strategies. The Carolina Pigmy Rattlesnake, Sistrurus m. miliarius, is one of two dwarf rattlesnake species. The subspecies ranges from eastern NC to nearly the southern tip of SC and into the northeastern portion of GA. In NC, where the species’ status is of Special Concern, fragmentation and land development have destroyed most of its historic range. Its small size also makes it highly susceptible to predation. In some parts of the range, commercial collection is another pressure the species …


About Fish, Humane Society Institute For Science And Policy Jan 2010

About Fish, Humane Society Institute For Science And Policy

Aquaculture Collection

Although a number of fish species have been studied in their freshwater life stages, further research on the behavior and habitat requirements of ocean-going fish is required. While fishes have historically been regarded as more “primitive” than other vertebrate groups, Rodriguez et al concluded that several memory and learning systems of bony fishes are noticeably similar to those of reptiles, birds, and mammals. When animals as small and under appreciated as fish display complex mating systems, parental care, and demonstrate the ability to traverse significant distances using olfactory and celestial cues, it is clear that previously established definitions of intelligence …


About Turkeys, The Humane Society Of The United States Jan 2010

About Turkeys, The Humane Society Of The United States

Agribusiness Collection

John James Audubon, a well-known bird expert and nature enthusiast, described wild turkeys as birds of great beauty. The history and origin of wild turkeys is uncertain, yet many share Audubon’s sentiment that the wild turkey is “one of the most interesting of the birds indigenous to the United States of America.” Today, wild turkeys can be found throughout the nation. Following the selection of the bald eagle as the American symbol, Benjamin Franklin remarked that the turkey was more “respectable”, and a “true original native”.


About Chickens, The Humane Society Of The United States Jan 2010

About Chickens, The Humane Society Of The United States

Agribusiness Collection

The chicken is the world’s most numerous domesticated bird, with over 52 billion farmed worldwide in 2008, rivaling the dog as the most ubiquitous domestic animal globally. These birds have fascinated scholars and researchers since the dawn of Western civilization, and recent studies are beginning to reveal the depths of their complexity and cognitive ability. According to Andrew F. Fraser, professor of veterinary surgery at Memorial University of Newfoundland, and Donald M. Broom, professor of animal welfare at University of Cambridge: “Those who have studied the behaviour of the domestic fowl in detail…, especially those who have looked at feral …


Behavior Comparisons Of Two Rearing Protocols For Whooping Cranes Raised By Costumed Caregivers And Trained For An Ultralight-Led Migration, Glenn H. Olsen, John B. French Jan 2010

Behavior Comparisons Of Two Rearing Protocols For Whooping Cranes Raised By Costumed Caregivers And Trained For An Ultralight-Led Migration, Glenn H. Olsen, John B. French

Proceedings of the North American Crane Workshop

Whooping crane (Grus americana) colts are raised at USGS Patuxent Wildlife Research Center, Laurel, Maryland for the first 40-60 days of a chick's life as part of the Whooping Crane Eastern Partnership (WCEP) ultralight-led reintroduction. Numbers raised for WCEP are increasing each year. Up to 2005, we raised whooping crane chicks in the Propagation Building where there are 10 indoor/outdoor pens, 8 full pens, and 2 half-size pens. In 2005 WCEP proposed increasing the number of colts reared to 20-24, numbers beyond the capacity of the facility. To accommodate this greater number of chicks, we modified several outdoor …


The Use Of High Frequency Gps Data To Classify Main Behavioural Categories In A Przewalski’S Horse In The Mongolian Gobi, Petra Kaczensky, Klaus Huber Jan 2010

The Use Of High Frequency Gps Data To Classify Main Behavioural Categories In A Przewalski’S Horse In The Mongolian Gobi, Petra Kaczensky, Klaus Huber

Erforschung biologischer Ressourcen der Mongolei / Exploration into the Biological Resources of Mongolia, ISSN 0440-1298

Behavioral observations of free ranging animals can provide important insight into many aspects of their biology but are not without problems. The recent development of GPS technology allows to remotely collect high precision location data at fixed intervals. We tested whether it is possible to classify the behavior of a Przewalski’s horse in the Mongolian Gobi into Resting, Grazing and Moving based on GPS locations collected at 15 minute intervals by comparing GPS data with direct observations. Although behavioral categories lasting for 15 minutes could by fairly reliably separated based on the distances covered between successive fixes, almost half the …


Behavior And Habitat Use Of Greater Sandhill Cranes Wintering In East Tennessee, David A. Aborn Jan 2010

Behavior And Habitat Use Of Greater Sandhill Cranes Wintering In East Tennessee, David A. Aborn

Proceedings of the North American Crane Workshop

An increase of eastern greater sandhill cranes (Grus canadensis tabida) stopping over and wintering at the Hiwassee Wildlife Refuge in east Tennessee has led to overcrowding at the refuge, resulting in crane use of private property. Proper management is needed to prevent the cranes from becoming a nuisance, increase the suitability of the refuge for waterfowl, and reduce the likelihood of a disease outbreak. Observations of cranes revealed that cranes spent 83% of their time foraging, alert, or preening. Sandhill cranes foraged primarily in agricultural and grassy fields, whereas mudflats and shallow water were used for preening and …


Prolonged Intensive Dominance Behavior Between Gray Wolves, Canis Lupus, L. David Mech, H. Dean Cluff Jan 2010

Prolonged Intensive Dominance Behavior Between Gray Wolves, Canis Lupus, L. David Mech, H. Dean Cluff

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Dominance is one of the most pervasive and important behaviors among wolves in a pack, yet its significance in free-ranging packs has been little studied. Insights into a behavior can often be gained by examining unusual examples of it. In the High Arctic near Eureka, Nunavut, Canada, we videotaped and described an unusually prolonged and intensive behavioral bout between an adult male Gray Wolf (Canis lupus) and a male member of his pack, thought to be a maturing son.With tail raised, the adult approached a male pack mate about 50 m from us and pinned and straddled this …


Observational Learning In Wild And Captive Dolphins, Deirdre Yeater, Stan A. Kuczaj Ii Jan 2010

Observational Learning In Wild And Captive Dolphins, Deirdre Yeater, Stan A. Kuczaj Ii

Psychology Faculty Publications

Many non-human species imitate the behavior of others, and dolphins seem particularly adept at this form of observational learning. Evidence for observational learning in wild dolphins is rare, given the difficulty of observing individual wild animals in sufficient detail to eliminate other possible explanations of purported imitation. Consequently, much of the evidence supporting observational learning in dolphins has involved animals in captive settings. This research suggests that dolphins have an affinity for mimicry, and that they are more successful at observational learning if they choose to imitate another rather than being asked to do so. These results, combined with those …


The Interictal State In Epilepsy And Behavior, Daniel Tice Barkmeier Jan 2010

The Interictal State In Epilepsy And Behavior, Daniel Tice Barkmeier

Wayne State University Dissertations

Epilepsy is one of the most common neurological diseases, affecting up to 1% of the world population. Epilepsy remains poorly understood and there are currently no medications to cure it. Patients with epilepsy have both seizures as well as another type of abnormal activity between seizures, known as interictal spikes. Interictal spikes have thus far been poorly researched, yet growing evidence supports an important role for them in epilepsy. In this project, we first show the high variability between reviewers in marking interictal spikes on intracranial EEG, and then develop and test an automated detection method to solve this problem. …


Female Humpback Whale (Megaptera Novaeangliae) Reproductive Class And Male-Female Interactions During The Breeding Season, Meagan E. Jones Jan 2010

Female Humpback Whale (Megaptera Novaeangliae) Reproductive Class And Male-Female Interactions During The Breeding Season, Meagan E. Jones

Antioch University Dissertations & Theses

This study examined whether female reproductive class (i.e., presence or absence of a calf) in humpback whales (Megaptera novaeangliae) affects female behavior and male-female interactions on the Hawaiian breeding grounds. From 2003-2008, 295 female-calf groups (with or without a male escort) and 256 female no-calf groups (with a male) were observed. Forty-one female no-calf groups were confirmed male-female pairs by genetic sexing or photo-identification. Focal follows of 36 male-female pairs and 50 female-calf groups (29 escorted and 21 unescorted female-calf groups) were analyzed for time budget and movement estimates. Relative fluke size (fluke photogrammetry, n=30) and sighting histories …