Karyotyping As A Method To Verify Phylogenetic Relationships Among The Enneacanthus Species, Ophelia A. Anderson

Theses and Dissertations

The Enneacanthus genus consists of three species of freshwater fish endemic to eastern North America: E. obesus (Banded Sunfish), E. gloriosus (Blue Spotted Sunfish), and E. chaetodon (Black Banded Sunfish). Previous studies using molecular data suggest that E. obesus and E. gloriosus are more closely related, with E. chaetodon as the most distantly related species in the genus. This study investigates the phylogenetic relationship among the Enneacanthus species, using karyotype characteristics - chromosome number, morphology and fundamental number - as comparative metrics. Chromosome spreads were obtained from scale and gill epithelium samples collected between 1993 and 1994. Prepared slides …

Behavioral Observations And Analysis Of Twix: The Cape Porcupine At The Lake Superior Zoo, Erin Davidson

Scripps Senior Theses

The three main goals of zoos are outlined as: education, conservation, and research. In our modern world, the focus of zoos has shifted away from simply housing animals, and towards how we can best promote proper animal welfare for individuals in human care. Animal welfare can be defined as the “mental health” of non-human species of fauna, and is greatly influenced by the stressors within the environment, as well as the option of choosing how to interact with and respond to the environment. Animals housed in zoos are presented with unique stressors that they often do not choose to experience, …

Effects Of Diet Composition On Physical Parameters In Danio Rerio, Isabella Hemphill

Williams Honors College, Honors Research Projects

With the use of zebrafish as a common model organism for human health, the reduction of confounding variables in their care is key. Many commercialized zebrafish diets are available for purchase, but each one consists of a different ratio of these crucial macronutrients. This study aimed to determine the effects long-term diets high in one macronutrient would have on the morphometrics, heart, liver, and protein content of zebrafish. Four diets were fed over a period of almost two years- high lipid, high carb, high protein, and a control diet. The control diet was the standard feed used in this lab, …

Exploring The Effects Of Road Salt On Earthworm Species, Alea Mry Koston

Williams Honors College, Honors Research Projects

This study examines the effects of two road salts, sodium chloride (NaCl) and calcium magnesium acetate (CMA), on the health of two earthworm species: Eisenia fetida and Lumbricus terrestris. Over a 4-week period, approximately 168 earthworms were exposed to varying concentrations of either NaCl, CMA, or mixed ratios of both, with a control group included for comparison. The experiment involved monitoring the changes in earthworm weight and measuring their contraction rates to determine physiological responses. Statistical analysis revealed no significant differences in weight or activity between NaCl and CMA treatments for either species. However, higher concentrations of NaCl resulted in …

Elongate Locomotors Show Consistent Torque Patterns Across Substrates, Spencer Bradford, Henry Astley

Williams Honors College, Honors Research Projects

The four main movements found in snakes are understood mainly due to external study; however, muscular forces and resulting torques are poorly known. The main research objective is to study the torque production on different substrates using a snakebot. The study will give a rudimentary model for torque production on different substrates. It can then be compared against biological systems to understand snake movement at a muscular level. This study used a snakebot, allowing direct assessment of the current being run through each of its motors (proportional to torque). Data was then examined using a Principal Component Analysis test for …

Development Of A Proposed Selective Medium For The Isolation And Differentiation Of P. Sordellii, Dimyana Bottros

Honors Undergraduate Theses

Clostridium, a genus of anaerobic, spore-forming bacteria, includes clinically and ecologically significant species like Clostridium difficile and Paeniclostridium sordellii. C. difficile causes antibiotic-associated colitis, while P. sordellii is linked to fatal infections and spontaneous abortions. Recent studies found both species in marine sediments, suggesting their role in environmental and wildlife health disturbances, including the 2013 mass mortality of over 300 manatees in the Indian River Lagoon. However, isolating and differentiating P. sordellii is challenging due to the lack of selective media optimized for its growth. This thesis proposes a novel selective medium tailored to P. sordellii’s physiological …

Development Of A Stable Isotope Method To Measure Protein Fractional Synthesis Rate In Equine Skeletal Muscle, Antoinette E. Foliart

Theses and Dissertations--Animal and Food Sciences

Muscle mass is regulated by the balance between muscle protein synthesis and degradation. Previous research in horses has shown that the signaling pathway that regulates protein synthesis is upregulated with increasing protein intake; however, whether this corresponds to an increase in muscle protein synthesis rates had not been previously explored. The objective of this study was to develop an isotopic method to measure muscle protein fractional synthesis rate (FSR) in horses following graded protein intake. Eight mature Thoroughbred horses were studied using a randomized cross-over design. Horses consumed meals containing 0 (No), 0.125 (Low), 0.25 (Med), or 0.5 (High) g …

Lives And Lessons Of Zoo Animals, Nicole Emlen

Undergraduate Theses, Professional Papers, and Capstone Artifacts

This project was an interdisciplinary museum exhibit that was displayed at the Montana Natural History Center between April 4 and May 12, 2025. It displays my photographs that I took of some of the animals I worked with during my summer 2024 internship at ZooMontana. In this project, I educate the public about three main functions of zoos--rescue, conservation, and education--through the stories and lives of four species. These animals all have unique stories that are important to share to improve public knowledge of why zoos are important and how we can improve relationships between humans and wildlife. Photography is …

Assessing Intra-Seasonal Changes In Summer Elk Diet Composition, Ava E. Window

Undergraduate Theses, Professional Papers, and Capstone Artifacts

Elk (Cervus canadensis) require adequate forage to fulfill their seasonal nutritional requirements. Elk undergo their highest nutritional demands during summer to support critical life functions such as late gestation, lactation, and juvenile growth. However, variations in plant phenology significantly influence the quality and quantity of forage available to elk during the summer season. As summer advances and plant communities mature, forage quality often declines, leading to a landscape that imposes greater nutritional constraints on elk. As a result, prior research on elk foraging habits has primarily focused on late summer. This has led to limited knowledge surrounding the …

Monograph Available: Urban Coyotes As A Source Of Conflict With Humans: An Evaluation Of Common Management Practices

Human–Wildlife Interactions

This is an announcement about the published monograph titled "Urban Coyotes as a Source of Conflict with Humans: An Evaluation of Common Management Practices." This publication, part of the HWI Monograph Series, is online and open access.

Effect Of Vibration Isolation During Transportation On Beef Sirloin Subprimal And Steak Quality, Kylie Nevada Krueger

Electronic Theses and Dissertations

The objective of this study was to determine the influence of vibration during transportation on beef sirloin water holding capacity, color, and tenderness. USDA Choice center-cut sirloin top butts (n = 45 cases) were collected at a commercial processing facility and randomly allocated to one of three treatments 1) regular pallet driven 241 km (Reg241), 2) regular pallet driven 644 km (Reg644), and 3) vibration isolation pallet driven 644 km (Iso644). All subprimals were transported at -2.2 °C (d0) and stored for 10 days to mimic industry practices for product aging. Following storage (d11), one subprimal per case was evaluated …

Fluke Egg Sedimentation Test Procedure, Department Of Primary Industries And Regional Development, Western Australia

Animal production and livestock factsheets

The department’s approved procedure for detecting trematode eggs and the Eimeria leuckarti sedimentation method (FEST) on faecal samples.

Estimating The Carbon Footprint Of Sheep Production In Western Australia, Tafesse Estifanos, Christophe D'Abbadie, Johnny Machon, Amir Abadi Abadi Ghadim, Gus Manatsa, Paul Mattingley, Ross Kingwell, Kevin Foster

Farm Systems and Economics published reports

Western Australia’s broadacre mixed farming systems combine cropping and sheep production and are the largest component of the state’s agricultural sector. The south west Western Australia (WA) agricultural area hosts about 98% of the state's sheep flock. The sheep industry contributed $1.5 billion to the state’s economy in 2021-22 and accounts for about a quarter of on-farm greenhouse gas (GHG) emissions. Most sheep industry emissions (over 80%) come from methane produced by the sheep. Despite this environmental impact, only 9.9% of Australian sheep producers have estimated their emissions, which limits the industry's ability to monitor and manage carbon footprints effectively. …

Investigating Relatedness Within Breeding Units Of The Highly Social Bushtit (Psaltriparus Minimus), Fiona Skye Connelly

Theses and Dissertations--Biology

Cooperative breeding systems represent an array of varied reproductive strategies and remain a topic of interest in behavioral ecology (i.e., Riehl 2013, Cornwallis et al. 2017). Different manifestations of cooperative breeding, such as cooperative polyandry, no doubt have unique evolutionary pressures tied to local ecologies which have led to their proliferation. Of particular interest are species that show flexibility in their use of reproductive strategies, wherein individuals may choose one strategy over another given a specific circumstance. Here, I used double-digest restriction site-associated DNA sequencing (i.e., ddRAD-seq) to investigate bushtit (Psaltriparus minimus) reproductive strategies and the relatedness between …

The Intermediate And Remote Effects Of Acute Juvenile Stress Upon Auditory Fear Learning, Recognition Memory, And Anxiety Measures In A Rodent Model Of Ptsd, Laura J. Baron

Electronic Theses & Dissertations (2024 - present)

Abstract

Early-life stress is a risk factor for increased susceptibility to stress- and trauma-related disorders, including Posttraumatic Stress Disorder (PTSD). PTSD is characterized by exposure to trauma that results in exaggerated and extinction-resistant fear responses that persist for over a month. Understanding how the severity of early-life stressors in animal models impacts adult behavior, cognition, and underlying neurobiology could aid in the development of more effective PTSD treatments.

Various early-life stress models, especially in infant rats, have been used to induce stress and assess PTSD like-symptoms. Different stressors impact short- and long-term behavior, with some effects influenced by developmental timing …

Using Algal Turf Scrubber Biomass As A Feed Source For Juvenile Eastern Oysters, Taofeek Saka Jimoh

College of Graduate Studies: Theses & Dissertations

Algal turf scrubber (ATS) is an engineered system that promotes natural wastewater treatment processes and improves water quality by passing a shallow stream of nutrient rich water over the surface of a gently sloped flow-way. Algae turf scrubber biomass is a combination of micro and macroalgae, as well as micro-invertebrates, ciliates, bacteria, and more. The overall aim of the study was to reduce the feeding cost of juvenile eastern oysters by replacing the commercial microalgae diet with ATS biomass. The experiment was designed to assess the viability of replacing commercial microalgal diet with ATS biomass at different substitution levels (0, …

Microbial Community Stabilization By Restored Oyster Reefs: Implications For Blue Carbon Storage, Natalie Boydstun

College of Graduate Studies: Theses & Dissertations

Coastal ecosystems provide critical carbon sequestration services, yet the microbial mechanisms underlying carbon storage remain poorly understood, particularly in non-vegetated habitats. This study investigated whether restored oyster reefs (Crassostrea virginica) enhance sediment carbon storage potential by stabilizing microbial communities and promoting efficient organic matter processing. I hypothesized that reef-mediated sediment stabilization and organic deposition would create conditions favoring microbial degradation of complex carbon compounds into stable soil organic matter, ultimately supporting anaerobic carbon storage. To test this, I compared sediment biogeochemistry, enzyme activity, and microbial community structure between three restored oyster reef sites and two unstructured control sites …

Movement And Activity Of Burbot, (Mizay; Lota Lota) In A Spring-Fed Minnesota Lake, Abigail M. Fountain

Biology Graduate Theses

Burbot (Mizay; Lota lota) are a relatively unmanaged freshwater fish, important in many local Indigenous cultures, but often overlooked or maltreated in research, conservation, and fishing practices. As such, relatively little research has been conducted on Burbot, despite ecological significance as an indicator species. A recent resurgence in popularity as a target species, however, has prompted new research to inform management policies. The objectives of this study were to summarize Burbot movement by identifying patterns in swimming activity and identify possible predictors of the movement observed. This study investigated the movements of 39 individuals implanted with acoustic transmitters in Bad …

Pygoscelis Penguins Prefer Different Oceanographic And Terrestrial Habitats During The Austral Summer, Katherine L. Gallagher, Rachel Herman, Katherine Walton, Michael S. Dinniman, Heather J. Lynch

OES Faculty Publications

Pygoscelis penguins interact with both terrestrial and marine environments, especially during the austral summer when they raise their chicks. Accessible ice-free ground spaces serve as locations for penguins to build colonies and successfully raise chicks while the surrounding marine environment serves as the foraging grounds for adult birds. Penguins must be able to navigate through the waters around their colonies to forage for Antarctic krill (Euphausia superba), their primary prey during the austral summer. Krill, whose distributions are driven partially by the surrounding physics and partially by their behaviors, must also be plentiful in the waters near the …

Effects Of Essential Oils Alone Or In Combination With Monensin In An In Vitro Model Of Ruminal Acidosis, Jorge L. Bonilla Urbina

Electronic Theses and Dissertations

The objective of this study was to evaluate the effect of essential oils and/or monensin on the concentration of volatile fatty acids (VFA), ammonia, and pH in a ruminal acidosis in vitro model. Red Angus steers (n = 4; BW = 435 ± 9 kg) with ruminal and duodenal cannulas were allocated in a 4 × 4 Latin square design. Treatments were 1) Fed no essential oils (RB) or monensin sodium (MON:CON); 2) Fed RB at a rate of 14 g daily with no Mon (RB+); 3) Fed no RB and fed Mon at 400 mg daily (MON+); 4) Fed …

An Edna Assessment Of The Diamond Darter (Crystallaria Cincotta) In Its Current And Historic Distribution Range, Cameron Moore Layne

Graduate Theses, Dissertations, and Problem Reports (ETD)

Environmental DNA (eDNA) can serve as a useful monitoring tool for cryptic and imperiled freshwater stream fishes. eDNA can fill knowledge gaps regarding species’ distribution, site abundance, and habitat usage. The last known stronghold of the Diamond Darter (Crystallaria cincotta) in the Elk River of West Virginia provides an excellent test case to examine the utility of this non-invasive method of surveying rare species. Diamond Darters are one of the most newly recognized freshwater fishes in North America and are among the most imperiled U.S. fishes, having been federally listed as endangered in 2013. The cryptic behavior and limitations of …

Longitudinal And Ensemble Modeling Of Beef Cattle Feed And Water Intakes, Nathan El Blake

Graduate Theses, Dissertations, and Problem Reports (ETD)

Beef cattle production remains a cornerstone of global food systems, providing highly bioavailable protein and essential micronutrients that are difficult to secure from plant-based foods alone. Yet this production is also resource-intensive, requiring substantial land, feed, and water inputs while contributing significantly to greenhouse gas emissions. Efficiency of dry matter intake (DMI) and water intake (WI) is central to both productivity and sustainability, but accurate measurement of these traits—particularly in grazing systems—remains a major limitation. National WI standards continue to rely on pen-level data collected in the 1950s, while most existing DMI prediction equations perform poorly when applied beyond the …

Tailwater Trout Population Dynamics, Connor Dylan Cunningham

Graduate Theses/Dissertations

Dams have been constructed throughout the world and provide several resources for humans (e.g., flood control, hydropower). These structures heavily alter the upstream and downstream environment. One consequence of these alterations is the creation of “tailwaters”, which can create cold-water habitat via hypolimnetic releases. In the southeast United States these tailwater environments are often managed for the recreational use of nonnative salmonids. The unnatural flow regimes of tailwaters affect the biotic and abiotic conditions and ultimately the dynamic rate functions (i.e., recruitment, growth, mortality) of the fish inhabiting them. As such, the goal of this research is to provide fisheries …

Use Of Fecal Inoculum From Easy And Hard Keeper Horses To Determine In-Vitro Digestibility Of Feedstuffs Used In Equine Diets, Leah Bills

Graduate Theses/Dissertations

Digestibility studies provide vital information for formulating ideal diets based on an animal’s ability to digest and absorb the available nutrients from the feed provided. The purpose of this study was to determine if there is a difference in the nutrient digestion of horses between those that have a difficult time maintaining a healthy body weight (body condition score at or below 5, hard keeper) and those that easily maintain a healthy body weight (body condition score of >5, easy keeper). Six mature geldings averaging 534.1 ± 47.1 kg were used in this study. During the experimental period horses were …

Spatiotemporal Variability In Invasive Carp Spawning Activity In Two Midwestern River Basins, Braden A. Whisler

Masters Theses

Across the United States, fisheries managers and agencies continue to battle the spread of invasive bigheaded carp (Hypophthalmichthys spp.). Although previous research has identified environmental parameters such as temperature and flow to be triggers of spawning activity, many of these studies focus on larger rivers, rarely focusing on the specific thresholds or timing of when a spawning event takes place. I utilized the differing hydrology of the Illinois and Wabash Rivers and their tributaries to examine the differences in environmental parameters needed to trigger bigheaded carp reproduction. Three mainstem sites and three tributaries of both basins were sampled …

Pharmacological Safety Profiling Of Nigella Sativa (Ns) And Hibiscus Sabdariffa (Hs) Extracts In Zebrafish (Danio Rerio) Embryos: An Integrative Vet-Pharmacotoxicological Evaluation Of Lc₅₀, Teratogenicity, And Developmental Outcomes, Ahmed E. A. Mostafa

Mansoura Veterinary Medical Journal

Herbal medicines are deeply rooted in Egyptian and Middle Eastern traditions, with Nigella sativa (NS) and Hibiscus sabdariffa (HS) being among the most widely consumed remedies. Despite extensive pharmacological evidence of their therapeutic benefits, systematic safety validation remains limited. Conventional toxicity assessments predominantly rely on rodents and higher vertebrates, which are costly, time-consuming, and ethically challenging. This gap necessitates alternative models that align with the 3R principles (Reduction, Replacement, Refinement). In this study, the zebrafish (Danio rerio) embryo model was employed to evaluate the developmental toxicity and median lethal concentration (LC50) of NS and HS aqueous extracts. Zebrafish embryos at …

Ocean Acidification Mitigation: Potential Expansion Of Sav Restoration Strategy In The Chesapeake Bay, Cree N. Taylor

Undergraduate Research Posters

Zostera marina is a species of marine macrophyte that has been largely prioritized in past submerged aquatic vegetation (SAV) restoration initiatives within the Chesapeake Bay because of its 1) previous abundance, 2) demonstrated carbon burial abilities, and 3) observed ability for meadows of Z. marina to ameliorate low pH conditions. Due to the temperature variability and poor water quality associated with anthropogenic disturbances in the Chesapeake Bay, Z. Marina beds have experienced several die-off events with limited capacity for regrowth. This systematic review explored the possible benefits of focusing future SAV recovery initiatives on ensuring a diverse variety of SAV …

Evaluation Of Blue Catfish Populations In Four Midwestern Power Cooling Reservoirs, Tyler Joseph Murray

Masters Theses

Blue Catfish (Ictalurus furcatus) are the largest catfish in North America and as such angler desire for trophy fisheries have grown in addition to harvest fisheries. The Illinois Department of Natural Resources (IDNR) has stocked Blue Catfish into several reservoirs in the state including four that have powerplants that utilize the water for cooling and discharge it back into the reservoir at increased temperatures. This study explored the potential impacts of these warm water effluents on Blue Catfish population demographics, as well as whether management changes need to be implemented in these systems. I sampled Powerton, LaSalle, Springfield, …

Shrubsteppe Birds Under Fire: Guiding Post-Wildfire Habitat Restoration In The Whiskey Dick And Quilomene Units, Washington, Kevin Clements

All Master's Theses

Bird species, globally, are declining at an alarming rate. Birds of the shrubsteppe are particularly vulnerable to habitat loss caused by agricultural development, invasive vegetation, and heightened wildfire frequency and severity. Fire and invasive vegetation create a positive feedback cycle that threatens complete conversion of shrubsteppe to grasslands. Restoring habitat following wildfire is a top priority for land managers. As bioindicators of ecosystem health, bird populations reflect landscape changes and restoration efficacy. I analyzed bird populations in the Whiskey Dick and Quilomene units of the L.T. Murray Wildlife Area in Kittitas County, Washington following the 2022 Vantage Highway fire. Using …

Prospective Use Of Detection Dogs As A Screening Tool For Mycoplasma Ovipneumoniae In Bighorn Sheep (Ovis Canadensis), Michele C. Lovara

Electronic Theses and Dissertations

Mycoplasma ovipneumoniae is a bacterium that can spill over from domestic sheep (Ovis aries) to bighorn sheep (Ovis canadensis), with effects in bighorn sheep ranging from low to no lamb recruitment to whole-herd all-age die-offs from resulting pneumonia. Fast and reliable pathogen or disease detection is essential for managing outbreaks in bighorn sheep and emergences in affected populations. Detection dogs are increasingly being enlisted to close gaps and further complement various disease, pathogen and virus research and response efforts. This thesis explores the use of conservation detection dogs as a potential tool for M. ovipneumoniae surveillance in bighorn sheep through …