Animal Sciences Commons^™

Ant Fauna (Hymenoptera: Formicidae) Of Central Anatolian Region Of Turkey, Kadri̇ Kiran, Celal Karaman

Turkish Journal of Zoology

The Central Anatolian part of Turkey was investigated with the aim of determining its Formicidae fauna. Specimens were collected by hand sampling and pitfall traps in 24 habitats in 383 different localities in Central Anatolia Region during 2001-2016. As a result, 137 species belonging to 34 genera were determined. Messor incorruptus Kuznetsov-Ugamsky, 1929 and Ponera testacea Emery, 1895 are new records for Turkish ant fauna. We excluded four species, Camponotus sylvaticus (Oliver, 1792), Messor meridionalis (André, 1883), Proformica striaticeps (Forel, 1911) and Tapinoma nigerrimum (Nylander, 1856), from Turkish ant fauna due to recent taxonomic data and distribution pattern. With these …

The Impact Of Chilling On Selected Attributes Of The Blowfly, Calliphora Vicina(Robineau-Desvoidy, 1830) (Diptera: Calliphoridae), Under Laboratory Conditions, Meltem Kökdener, Nevran Eylem Akman Gündüz, Ünal Zeybekoğlu

Turkish Journal of Zoology

The purpose of this study was to evaluate the effects of four different chilling periods, varying from 24 to 120 h, on the mean survival rate, larval and pupal development durations and the pupal and adult weight of Calliphora vicina (Robineau-Desvoidy) (Diptera: Calliphoridae) under laboratory conditions at 4 ºC in 2018 at Ondokuz Mayıs University. Chilling reduced the survival rate of the pupae of all developmental stages. The adult eclosion rate of first instar larvae and pupae was lower, especially after 72 and 120 h chilling periods, but it increased for the second instar, third instar and postfeeding larvae with …

First Report On Mild Insecticide Resistance In Newly Established Aegean Aedes Albopictus Populations Of Turkey, Sare İlknur Yavaşoğlu

Turkish Journal of Zoology

The invasive nature of Aedes albopictus, a competent vector of dengue, zika, yellow fever, and chikungunya viruses, has spread to many parts of the world. To date, there have been no report regarding the insecticide resistance status of Aedes albopictus populations along the Aegean coasts of Turkey. This study aims to confirm the occurrence of Aedes albopictus populations in the Aegean region of Turkey and test their susceptibility against different insecticide classes. Bioassay for susceptibility to Dikloro difenil trikloroetan (DDT) (4%), propoxur (0.1%), fenitrothion (1%), bendiocarb (0.1%), permethrin (0.75%), and deltamethrin (0.05%) of each population was assessed through the World …

Capoeta Kosswigi Karaman, 1969 A Junior Synonym Of Capoeta Damascina(Valenciennes, 1842) (Teleostei: Cyprinidae), Erdoğan Çi̇çek, Soheil Eagderi, Burak Seçer, Sevi̇l Sungur

Turkish Journal of Zoology

Capoeta kosswigi was described from Lake Van basin, Turkey, being close to C. damascina and C. umbla. It is distinguished from them by a pointed snout and a small horseshoe-shaped mouth, and differing from C. damascina by having smaller scales, and from the C. umbla by processing larger scales. In this study, we tested the suggested synonymy hypothesis of C. kosswigi and C. damascina by comparing the morphometric and meristic data. The results revealed that all morphometric and meristic characters of C. kosswigi overlap with those of C. damascina. In line with previous molecular studies that COI, cytb and 16S …

Exploring The Dynamics Of Small Pelagic Fish Catches In The Marmara Sea In Relation Tochanging Environmental And Bio-Optical Parameters, Eki̇n Akoğlu

Turkish Journal of Zoology

The Marmara Sea has a large drainage basin that includes cities with heavy industrialisation and agricultural land use. Therefore, it is under the influence of significant anthropogenic activities that adversely affect the well-being of its ecosystem. The Marmara Sea is also an important fishing ground with many commercially important fish species. These fish species are also under the influence of the adverse impacts of anthropogenic activities. Fisheries catches in the Marmara Sea had experienced severe fluctuations since the onset of the 2000s. In this study, possible underlying reasons for these fluctuations were investigated between 2000 and 2019 using remotelysensed environmental …

Population Genetics Of A Reintroduced Fisher (Pekania Pennanti) Population In West Virginia, Caroline E. Harms

Graduate Theses, Dissertations, and Problem Reports (ETD)

Fishers (Pekania pennanti) were reintroduced in West Virginia in 1969 with no population wide genetic analysis ever having been conducted. Genetic analysis of reintroduced populations is vital to assess genetic diversity as an indicative of population viability. My objective was to collect fisher samples throughout West Virginia, nearby states where fisher may be migrating (PA and NY), and the New Hampshire source population to provide a full genetic interpretation of the reintroduced West Virginia population. Genetic analysis was used to measure genetic diversity, admixture or delineation of subpopulations, and effective population size. Sample location and genetic data were …

Effect Of Diet, Age, And Genetics On Rainbow Trout Growth And Fillet Quality, Bahareh Adami

Graduate Theses, Dissertations, and Problem Reports (ETD)

In this study, analyses were conducted to compare fillet quality attributes of 4 rainbow trout families (40, 102, 159, and 187) fed fish meal (FM) and a fishmeal free diet (FMF), to two age endpoints, 9 and 11 months.

Previous studies with rainbow trout have evaluated the effect of age, diet, and family on growth performance and fillet quality. However, a comprehensive analysis of variables is lacking for rainbow trout, one of the main aquatic foods for domestic and international consumers.

Separable muscle as a percent whole body weight was not affected by diet, family, age, and the interaction of …

Age, Growth, And Mortality Of Atlantic Tripletail In The North-Central Gulf Of Mexico, Amanda E. Jefferson, Matthew B. Jargowsky, Meagan N. Schrandt, Pearce T. Cooper, Sean P. Powers, John Dindo, J. Marcus Drymon

University Faculty and Staff Publications

In the southeastern USA and the Gulf of Mexico (GOM), Atlantic Tripletail Lobotes surinamensis are increasingly targeted by recreational anglers, indicating that stock status should be assessed. A critical need for such assessments is age-specific data; however, previous studies have drawn conflicting conclusions regarding the most appropriate structure for aging. Moreover, growth parameters and mortality rates for GOM Atlantic Tripletail are unknown. Therefore, the goals of this study were to (1) evaluate sagittal otoliths and first dorsal spines as aging structures; (2) model combined and sex-specific growth; and (3) estimate mortality rates for GOM Atlantic Tripletail. From 2012 to 2019, …

Documentation Of Atlantic Tarpon (Megalops Atlanticus) Space Use And Move Persistence In The Northern Gulf Of Mexico Facilitated By Angler Advocates, J. Marcus Drymon, Matthew B. Jargowsky, Michael A. Dance, Mitchell Lovell, Crystal L. Hightower, Amanda E. Jefferson, Andrea M. Kroetz, Sean P. Powers

University Faculty and Staff Publications

Atlantic tarpon (Megalops atlanticus, hereafter tarpon) are facing a multitude of stressors and are considered Vulnerable by the IUCN; however, significant gaps remain in our understanding of tarpon space use and movement. From 2018 to 2019, citizen scientists facilitated tagging of 23 tarpon with SPOT tags to examine space use and movement across the northern Gulf of Mexico. Movement-based kernel densities were used to estimate simplified biased random bridge-based utilization distributions and a joint move persistence model was used to estimate a behavioral index for each fish. Tarpon showed consistent east–west movement from the Alabama/Florida border to Louisiana, and utilization …

Effects Of Human-Animal Interactions On Affect And Cognition, Elise L. Thayer, Jeffrey R. Stevens

Jeffrey Stevens Publications

Human-animal interaction has clear positive effects on people’s affect and stress. But less is known about how animal interactions influence cognition. We draw parallels between animal interactions and exposure to natural environments, a research area that shows clear improvements in cognitive performance. The aim of this study is to investigate whether interacting with animals similarly enhances cognitive performance, specifically executive functioning. To test this, we conducted two experiments in which we had participants self-report their affect and complete a series of cognitive tasks (long-term memory, attentional control, and working memory) before and after either a brief interaction with a dog …

Diets Of Double-Crested Cormorants In The Lake Winnebago System, Wisconsin, Ryan P. Koenigs, Daniel J. Dembkowski, Charles D. Lovell, Daniel A. Isermann, Adam D. Nickel

United States Department of Agriculture Wildlife Services: Staff Publications

Double-crested cormorant Phalacrocorox auritus Lesson (cormorant) populations have increased throughout the Great Lakes region of North America causing concern related to the impact of cormorant predation on fish communities. A recent decline in yellow perch Perca flavescens (Mitchill) abundance within the Lake Winnebago System, Wisconsin, USA, prompted an assessment of cormorant diets to evaluate potential effects of cormorant predation on the sportfish community. Diets were collected from 883 cormorants (417 from Lake Winnebago and 466 from Lake Butte des Morts) between 2015 and 2017. Cormorant diets on both waterbodies consisted mostly of freshwater drum Aplodinotus grunniens Rafinesque and gizzard shad …

Records Of Hippoboscidae From Mongolian Birds Of Prey With Checklist, Update, Matthias Jentzsch, Benjamin Meissner, Nayamsuren Batsaikhan, Annegret Stubbe, Michael Stubbe

Erforschung biologischer Ressourcen der Mongolei / Exploration into the Biological Resources of Mongolia, ISSN 0440-1298

The hippoboscid species Ornithophila gestroi has been rediscovered from Mongolia. It is the easternmost of all the known occurrences. Specimens were collected from Amur Falcon Falco amurensis, Saker Falcon Falco cherrug, Lesser Kestrel Falco naumanni, Eurasian Hobby, Falco subbuteo, Common Kestrel Falco tinnunculus Linnaeus, 1758 and Black Kite Milvus migrans in a breeding area located in southern Mongolia. Amur Falcon, Eurasian Hobby and Black Kite were identified as hosts of O. gestroi for the first time.

Jaguars And Pumas Exhibit Distinct Spatiotemporal Responses To Human Disturbances In Colombia’S Most Imperiled Ecoregion, Joe J. Figel, Sebastián Botero-Cañola, Juan David Sánchez-Londoño, Javier Racero-Casarrubia

University of Nebraska State Museum: Mammalogy Papers

Abstract

Coexistence of sympatric felids is facilitated by mutual avoidance and the partitioning of habitats, prey, and time. Anthropogenic disturbances disrupt this coexistence in fragmented landscapes, potentially triggering cascading influences in ecological communities. We used photographic data from 8,717 trap nights (November 2014–June 2016) at 87 camera trap sites in Colombia’s middle Magdalena River basin to compare spatiotemporal overlap among jaguars (Panthera onca), pumas (Puma concolor), their prey, and humans, at sites of high and low disturbance, as determined by the human influence index. Human disturbance events (e.g. domestic dogs, livestock, and humans, including armed hunters) …

Evidence On The Effectiveness Of Small Unmanned Aircraft Systems (Suas) As A Survey Tool For North American Terrestrial, Vertebrate Animals: A Systematic Map Protocol, Jared A. Elmore, Michael F. Curran, Kristine O. Evans, Sathishkumar Samiappan, Meilun Zhou, Morgan B. Pfeiffer, Bradley F. Blackwell, Raymond B. Iglay

United States Department of Agriculture Wildlife Services: Staff Publications

Background: Small unmanned aircraft systems (sUAS) are replacing or supplementing manned aircraft and groundbased surveys in many animal monitoring situations due to better coverage at finer spatial and temporal resolutions, access, cost, bias, impacts, safety, efficiency, and logistical benefits. Various sUAS models and sensors are available with varying features and usefulness depending on survey goals. However, justification for selection of sUAS and sensors are not typically offered in published literature and existing reviews do not adequately cover past and current sUAS applications for animal monitoring nor their associated sUAS model and sensor technologies, taxonomic and geographic scope, flight conditions and …

Recolonizing Carnivores: Is Cougar Predation Behaviorally Mediated By Bears?, Kristin N. Engebretsen, Jon P. Beckmann, Carl W. Lackey, Alyson M. Andreasen, Cody Schroeder, Pat Jackson, Julie K. Young

United States Department of Agriculture Wildlife Services: Staff Publications

Conservation and management efforts have resulted in population increases and range expansions for some apex predators, potentially changing trophic cascades and foraging behavior. Changes in sympatric carnivore and dominant scavenger populations provide opportunities to assess how carnivores affect one another. Cougars (Puma concolor) were the apex predator in the Great Basin of Nevada, USA, for over 80 years. Black bears (Ursus americanus) have recently recolonized the area and are known to heavily scavenge on cougar kills. To evaluate the impacts of sympatric, recolonizing bears on cougar foraging behavior in the Great Basin, we investigated kill sites of 31 cougars between …

Assessing Pace-Of-Life: Comparing Metabolism And Behavior In Tarantulas, Haley Vanscoyoc

Master's Theses and Doctoral Dissertations

Pace-of-Life Syndrome (PoLS) links physiology, behavior, and life history to understand how an organism lives and is under-researched in many invertebrate groups. In this study I used five tarantula species to assess if they follow the predictions of PoLS. I compared individual species as well as Old- and New-World lineages and solitary and sub-social species. I predicted that Old- World and sub-social species would have higher resting metabolic rates (RMRs) and activity levels compared to New-World and solitary species. RMRs were measured using an open-flow respirometry system, and a classic open-arena test was used to assess activity level. Spearman’s correlation …

Text Mining To Identify The Origin Of Chronic Wasting Disease, G. Webb

Faculty Research, Scholarly, and Creative Activity

Chronic wasting  disease (CWD)  is  a 100  percent  fatal,  prion  disease of  deer  that  has  the potential  to decimate  the  deer  population and  jump to  the  human  population.   A  simple  Google  search on  the  “origin of  chronic wasting  disease” yielded  a total  of  56  relevant  articles.   Of  these,  75  percent  report  that  the origin  is  unknown,  19.6  percent  report  that  the  disease  may  have  originated  in  a  Fort  Collins,  Colorado, government  research facility, and 5.4 percent  report  other  possible  origins.  Government  sources  reported the  Fort  Collins  theory  4.3 percent  of  the  time  while  non-governmental  sources,  such  as news articles, reported …

Scaling The Effects Of Ocean Acidification On Coral Growth And Coral-Coral Competition On Coral Community Recovery, Nicolas R. Evensen, Yves-Marie Bozec, Peter J. Edmunds, Peter J. Mumby

Biological Sciences Faculty Publications

Ocean acidification (OA) is negatively affecting calcification in a wide variety of marine organisms. These effects are acute for many tropical scleractinian corals under short-term experimental conditions, but it is unclear how these effects interact with ecological processes, such as competition for space, to impact coral communities over multiple years. This study sought to test the use of individual-based models (IBMs) as a tool to scale up the effects of OA recorded in short-term studies to community-scale impacts, combining data from field surveys and mesocosm experiments to parameterize an IBM of coral community recovery on the fore reef of Moorea, …

Global Extinction Risk For Seahorses, Pipefishes And Their Near Relatives (Syngnathiformes), Riley A. Pollom, Gina M. Ralph, Caroline M. Pollock, Amanda C.J. Vincent

Biological Sciences Faculty Publications

Few marine taxa have been comprehensively assessed for their conservation status, despite heavy pressures from fishing, habitat degradation and climate change. Here we report on the first global assessment of extinction risk for 300 species of syngnathiform fishes known as of 2017, using the IUCN Red List criteria. This order of bony teleosts is dominated by seahorses, pipefishes and seadragons (family Syngnathidae). It also includes trumpetfishes (Aulostomidae), shrimpfishes (Centriscidae), cornetfishes (Fistulariidae) and ghost pipefishes (Solenostomidae). At least 6% are threatened, but data suggest a mid-point estimate of 7.9% and an upper bound of 38%. Most of the threatened species are …

Effects Of Weather On Foraging Success And Hunting Frequency In Winter-Irruptive Snowy Owls (Bubo Scandiacus) In Upstate New York, Russell E. Winter, William M. Shields

Biological Sciences Faculty Publications

The effects of weather on an individual can often alter the population dynamics of a species. Knowledge of how weather influences individual behavior is therefore essential in understanding its full impact in the context of population ecology. Snowy Owls (Bubo scandiacus) exhibit expensive long-distance migrations in winters following population irruptions. During irruptive movements, many owls migrate past the southernmost extent of their traditional wintering grounds, the mechanism for which is still debated. We propose and test the “milder climate” hypothesis; Snowy Owls wintering in lower latitudes are better able to meet their metabolic demands due to higher temperatures …

Advancing Applied Research In Conservation Criminology Through The Evaluation Of Corruption Prevention, Enhancing Compliance, And Reducing Recidivism, Jessica S. Kahler, Joseph W. Rivera, Zachary T. Steele, Pilar Morales-Giner, Christian J. Rivera, Carol F. Ahossin, Ashpreet Kaur, Diane J. Episcopio-Sturgeon

Biological Sciences Faculty Publications

Concomitant with an increase in the global illegal wildlife trade has been a substantial increase in research within traditional conservation-based sciences and conservation and green criminology. While the integration of criminological theories and methods into the wildlife conservation context has advanced our understanding of and practical responses to illegal wildlife trade, there remain discrepancies between the number of empirical vs. conceptual studies and a disproportionate focus on a few select theories, geographical contexts, and taxonomic groups. We present three understudied or novel applications of criminology and criminal justice research within the fields of fisheries, forestry, and wildlife conservation. First, we …

Common Raven Nesting And Spatial Distancing On Power Lines In Southeast Idaho, Usa, Quinn R. Shurtliff, Jericho C. Whiting

Human–Wildlife Interactions

Common raven (Corvus corax; raven) abundance and distribution have increased in western North America in recent decades, facilitated by anthropogenic subsidies and other environmental changes. Electrical power transmission line structures provide nesting substrates for ravens. When these structures transect landscapes where natural nest substrates are limited, they may facilitate raven predation on eggs and juveniles of sensitive avian and reptile species. Little information is available regarding raven nest density trends on adjacent power lines or how raven territorial behavior influences spatial partitioning of nests, and hence, nest density. This knowledge would be valuable for managers of sensitive prey …

Common Raven Impacts On The Productivity Of A Small Breeding Population Of Snowy Plovers, Matthew J. Lau, Benjamin H. Becker, David T. Press

Human–Wildlife Interactions

Common ravens (ravens; Corvus corax), an adaptable, synanthropic generalist, have thrived coincident with increasing human landscape modifications and fragmentation, consequently affecting their prey, which are often sensitive native and protected species. Ravens are a conservation concern for the protected western snowy plover (plover; Charadrius nivosus nivosus), causing low nest and chick survival in some breeding areas along the Pacific coast of North America. We used a long-term dataset from a breeding snowy plover monitoring program in Point Reyes National Seashore (PRNS) to investigate potential impacts of ravens on snowy plover nest and fledging success. Between 2002 and 2020, …

Demography, Morphometrics, And Stomach Contents Of Common Ravens Examined As A Result Of Controlled Take, Corinne M. Gibble, Kriss K. Neuman, Jessie Beck

Human–Wildlife Interactions

Common ravens (Corvus corax; ravens) are known nest predators that have the ability to negatively impact nesting birds, including imperiled species of seabirds and shorebirds. We conducted systematic necropsies of ravens that were lethally controlled in Monterey Bay, California, USA during 2013–2015, in or near western snowy plover (Charadrius nivosus nivosus) nesting areas, in an effort to better understand body condition, overall health, and diet of individual ravens. Raven predation of snowy plover nests has increased over the years in the Monterey Bay study area, and lethal removal of ravens has been employed to reduce predation. …

A Rapid Assessment Function To Estimate Common Raven Population Densities: Implications For Targeted Management, Brianne E. Brussee, Peter S. Coates, Shawn T. O'Neil, Seth J. Dettenmaier, Pat J. Jackson, Kristy B. Howe, David Delehanty

Human–Wildlife Interactions

Common raven (Corvus corax; raven) populations have increased over the past 5 decades within the western United States. Raven population increases have been largely attributed to growing resource subsidies from expansion of human enterprise. Concomitantly, managers are becoming increasingly concerned about elevated adverse effects on multiple sensitive prey species, damage to livestock and agriculture, and human safety. Managers could benefit from a rapid but reliable method to estimate raven densities across spatiotemporal scales to monitor raven populations more efficiently and inform targeted and adaptive management frameworks. However, obtaining estimates of raven density is data- and resource-intensive, which renders …

A Novel Technique To Improve Capture Success Of Common Ravens, Lindsey R. Perry, Terrah M. Owens, Zachary T. Slick, Jimmy D. Taylor, Jonathan B. Dinkins

Human–Wildlife Interactions

Traditional trapping techniques for common ravens (Corvus corax; raven) require significant effort, often produce low capture rates, and cannot be used in some situations. We designed a 3-m noose pole to secure ravens from nocturnal roost locations while using a strobe spotlight to temporarily disorient them. We collected measures of trapping efficiency and contrasted them with padded leghold traps also used in the study. We effectively implemented our noose pole method in July and August of 2018, 2019, and 2020 in the Baker and Cow Lakes sage-grouse (Centrocercus urophasianus) Priority Areas of Conservation in eastern Oregon, …

A Comprehensive Review On Genetic Diversity And Phylogenetic Relationships Amongnative Turkish Cattle Breeds Based On Microsatellite Markers, Eymen Demi̇r, Taki̇ Karsli, Murat Soner Balcioğlu

Turkish Journal of Veterinary & Animal Sciences

Many local breeds belonging to different livestock species such as cattle are in danger of extinction, since farmers prefer high-yielding breeds as well as intensive selection pressure. This fact leads to conduct suitable breeding and conservation programs in worldwide. Revealing genetic diversity is the first step for applying breeding and conservation programs. In this respect, this is the first comprehensive study aimed to review current genetic diversity and phylogenetic relationships among native Turkish cattle breeds based on previous microsatellite marker studies. Compared to other local cattle breeds reared in different regions of the world, native Turkish cattle breeds hold a …

Effect Of Eggshell Temperatures On Hatching Performance, Egg Production, And Bonemorphology Of Laying Hens, Serdar Kamanli, Zi̇şan Servet Yalçin, Ahmet Nuri̇ Taşdemi̇r, Bülent Tarim, Engi̇n Tülek, Hüseyi̇n Aygören, Öğünç Meral, Bi̇rkan Karsli, Tansel Ansal Balci, Mehmet Bozkurt

Turkish Journal of Veterinary & Animal Sciences

The present study aims to evaluate the effect of lower or higher eggshell temperatures from days 7 to 21 on hatching performance, bone and blood parameters, and egg production along with the age of laying chickens. A total of 3150 eggs obtained from a white egg-type breeder were divided randomly into 3 groups of 1050 eggs. From 7 to 21 days, eggs were incubated at one of three eggshell temperatures: Control (IC) 37.5 °C, low (IL) 36.9 °C, and high (IH) 38.5 °C. At hatch, chicks were weighed, and blood was collected to measure total Ca, inorganic P, and ALP …

The Curative Effects Of Dietary Yeast Beta-1,3/1,6-Glucan On Oxidative Stress And Apoptosis In Laying Quails (Coturnix Coturnix Japonica) Exposed To Lead, İsmai̇l Seven, Pinar Tatli Seven, Seda İflazoğlu Mutlu, Gözde Arkali, Nurgül Bi̇rben, Aslihan Sur Arslan, Mesut Aksakal

Turkish Journal of Veterinary & Animal Sciences

The objective of this study was to investigate the effects of supplementation of beta-glucan to the basal diet of laying quails exposed to lead (Pb) toxicity. A total of 112 birds were randomly divided into 4 groups. The first group was fed a corn-soybean based basal diet without any supplementation (control). The second (Pb) and third (beta-glucan) groups were supplemented with 100 mg/kg of Pb (as Pb acetate) and 100 mg/kg of beta-glucan, respectively. The fourth group (Pb + beta-glucan) was fed a basal diet supplemented with 100 mg/kg of Pb and 100 mg/kg of beta-glucan. It was determined that …

Pelvic Limb Thermography In Dogs Submitted To Different Thermotherapy Modalities, Samyla Paiva De Albuquerque, Orimacelio Da Costa Martins, Ariel De Aguiar, Lorena Oliveira Silva, Acácio Duarte Pacheco, Lourdes Marina Bezerra Pessoa, Luís Eduardo Maggi, Soraia Figueiredo De Souza

Turkish Journal of Veterinary & Animal Sciences

Veterinary physiatry is an area in need of further studies and a discipline in which the protocols used in the veterinary clinical routine are often extrapolated from studies in medicine. This study aimed to evaluate the effectiveness of different types of thermotherapy and massage, through thermography, in promoting heating or cooling in the pelvic limb of healthy dogs. Five healthy adult dogs (male and female) of different breeds were subjected to 5 treatments: G1: massage without oil; G2: oil massage; G3: heating with a thermal bag at 43 ± 1 °C; G4: cryotherapy with ice pack; and G5: therapeutic ultrasound. …