Animal Sciences Commons^™

Effects Of Invasive Nilgai Antelope (Boselaphus Tragocamelus ) Browsing On Black Mangrove (Avicennia Germinans) In South Texas: A Study On Microenvironmental, Physiological, Structural, And Reproductive Parameters, Tianna Picquet

Theses and Dissertations

Mangroves are important for coastal systems because they provide habitat for fish and bird species, mitigate erosion, and create an area for carbon sequestration. Along the Gulf of Mexico, at the southern tip of Texas, recent observations have shown that A. germinans stands (the dominant species in Texas) are being browsed on by invasive nilgai antelope (Boselaphus tragocamelus). The antelope were introduce to the south Texas region via King Ranch in the 1930’s and have since significantly increased in population and distribution. Previously thought to be grazers, B. tragocamelus were captured on camera utilizing A. germinans as a …

The Study Of Alcoholic Liver Diseases, Seun Emmanuel Owoseni

Electronic Theses and Dissertations

Excessive alcohol consumption is the primary contributing factor in the development of alcoholic liver diseases (ALD). Nicotine contained in tobacco is a major addictive alkaloid, which enhances the effects of ALDs. The major enzyme involved in nicotine metabolism is cytochrome P450 2A5 (CYP2A5) which is produced in the liver. Alcohol can stimulate the CYP2A5 enzyme. We utilized cyp2a5-/- knockout mice in this research to examine the effects of CYP2A5.

The cyp2a5-/- mice and wild-type (WT) mice were fed liquid ethanol diet with or without nicotine to induce ALD. Nicotine enhancing effects on ALD were observed in WT mice but not …

Efficacy Of Natural Compounds With Novel Carrier Systems For Controlling Campylobacter Jejuni In Post-Harvest Poultry, Sandip Shrestha

Graduate Theses and Dissertations

Campylobacter jejuni, a leading cause of bacterial gastroenteritis worldwide, is strongly associated with the consumption and/or mishandling of raw contaminated poultry products. Thus, interventions aiming to reduce C. jejuni counts on poultry products could greatly reduce the incidence of human campylobacteriosis. In the first study, the efficacy of a generally recongnized as safe (GRAS) compound, carvacrol (CR; derived from oregano oil), as an antimicrobial wash treatment to reduce C. jejuni on chicken skin was evaluated. Three delivery systems of CR: suspension, emulsion and nanoemulsion were used. C. jejuni counts were reduced up to 4 log10 cfu/sample by 2% dose of …

Varying Dataset Resolution Alters Predictive Accuracy Of Spatially Explicit Ensemble Models For Avian Species Distribution, Claire M. Curry, Jeremy D. Ross, Andrea Contina, Eli S. Bridge

School of Integrative Biological & Chemical Sciences Faculty Publications

Species distribution models can be made more accurate by use of new “Spatiotemporal Exploratory Models” (STEMs), a type of spatially explicit ensemble model (SEEM) developed at the continental scale that averages regional models pixel by pixel. Although SEEMs can generate more accurate predictions of species distributions, they are computationally expensive. We compared the accuracies of each model for 11 grassland bird species and examined whether they improve accuracy at a statewide scale for fine and coarse predictor resolutions. We used a combination of survey data and citizen science data for 11 grassland bird species in Oklahoma to test a spatially …

Temperature And Polyunsaturated Fatty Acid’S Effect On Daphnia Magna Reproduction, Mark Albright

Electronic Theses and Dissertations

Organisms adapt to their environments by adjusting their biochemistry and physiology; such adaptation is limited by resource availability and physiological constraints. The freshwater crustacean Daphnia magna inhabits a wide range of environments and must survive and reproduce within a range of temperatures. One limit to low-temperature adaptation is thought to be the availability of unsaturated fatty acids necessary to maintain proper fluidity of cellular membranes. D. magna maintained at 10 ºC on a diet poor in unsaturated fatty acids have been observed to produce clutches that fail to develop. However, this has not been observed on a diet rich in …

Development And Application Of A Reverse Genetics System For Zika Virus, Jordan C. Frank

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Zika virus (ZIKV) has emerged in many regions of the world, with infection outcomes spanning from no apparent illness to crippling nervous system disease. ZIKV and its close relatives, West Nile virus, Japanese encephalitis virus, dengue virus, and yellow fever virus are primarily transmitted by mosquitoes. Three ZIKVs were selected: MR-766 (Uganda, 1947), P6-740 (Malaysia, 1966), and PRVABC-59 (Puerto Rico, 2015), whose place of origin and time of isolation differ substantially. Stable, complementary DNA (cDNA) copies of the three ZIKV RNA genomes were cloned to examine the significance of viral and host genetic variations in directing ZIKV infection outcomes. Using …

Identification Of Sperm Chromatin Proteins As Candidate Markers Of Stallion Fertility, Chelsea C. Ketchum

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

During spermatogenesis, histones are largely replaced by transition proteins and protamines in normal stallions. Incomplete nucleoprotein exchange results in the abnormal retention of histones and transition proteins, which is an indicator of poor sperm quality. Equine nucleoprotein exchange has not previously been investigated in detail, so that equine sperm chromatin quality problems, which are often responsible for poor breeding performance of stallions, are not well understood. In order to characterize chromatin remodeling events in stallion spermatogenesis and to identify proteins indicative of sperm chromatin defects, such as excessive amounts of histones, we identified antibodies that recognize equine testis-specific proteins of …

Efficacy Of Translocation As A Management Tool For Urban Mule Deer In Utah, Channing R. Howard

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

An increase in urbanization in the United States has led to an increase in human-wildlife interactions with deer (Odocoileus spp.) which have been able to adapt and thrive in these urban environments. In Utah, urbanization has occurred along the Wasatch Front which was once traditional mule deer (Odocoileus hemionus) winter range habitat. This urban expansion coupled with an increasing use of these urban areas by mule deer, have led to increasing conflicts with deer. Overabundant urban deer have led to increased concerns over safety from deer-vehicle collisions, and damage to personal property including gardens and landscaping. …

Influence Of Environmental Variables On Survival Rates Of Pronghorn (Antilocapra Americana) Neonates Across Idaho, Brett R. Panting

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study was completed to better understand pronghorn antelope (Antilocapra americana) populations found throughout Idaho. Antelope were studied in three separate and distinct study areas. The Big Desert, Camas Prairie, and Little Lost and Pahsimeroi valleys were all selected as study sites. Idaho Department of Fish and Game (IDFG) is concerned with current pronghorn populations found throughout Idaho. Pronghorn are a valued big game species found in Idaho. Increasing pronghorn populations in Idaho is a focus of IDFG.

We captured and VHF-collared pronghorn fawns found in our three study areas. Fawns were monitored daily with telemetry equipment for …

Assessing The Effects Of Genetic Selection For Porcine Circovirus Type 2 Resistance Or Feed Additives On Nursery And Grow-Finish Pig Performance, Dana Michael Van Sambeek

Department of Animal Science: Dissertations, Theses, and Student Research

The swine industry is ever evolving as it strives to produce healthy, wholesome pork for consumers. As such, the industry is constantly looking for ways to improve production and reduce costs. This includes using novel feed ingredients to reduce cost and improve animal health, genetic selection for increased performance, and vaccines to attenuate or prevent illness. Research plays a significant role in assessing the effectiveness of these strategies. Overall, the objective of this thesis was to assess how genetic selection, vaccines, and feed additives may impact growth performance, health, nutrient digestibility, and the microbiome.

In Chapter 2, pigs were either …

Impact Of Ethanol Process Changes On Distillers’ Grains For Beef Cattle, Shelby A. Garland

Department of Animal Science: Dissertations, Theses, and Student Research

Distillers grains plus solubles (DGS) have been widely utilized in beef cattle diets. Fractionation of the corn kernel pre- and post-fermentation has changed the composition of DGS and allowed for the production of other feed byproducts over time. The use of fractionated DGS and other feed byproducts from the ethanol industry has not been heavily researched in beef cattle diets. Three experiments were conducted to evaluate the effect of feeding high protein distillers grains (HiPro DDG) and corn bran plus solubles (Bran + Solubles) in beef cattle diets. Experiment 1 evaluated the effect of byproduct type on finishing performance and …

Quality Effects On Beef From Cattle Fed High-Protein Corn Distillers' Grains Or Other Ethanol By-Products, Kellen B. Hart

Department of Animal Science: Dissertations, Theses, and Student Research

The objective of this study was to evaluate the effects of feeding high protein corn distillers grains on fresh beef quality. Steers (n = 300) were fed one of five diets (6 pens with 10 head/pen): a corn control, 40% high protein dry distillers grains plus solubles (HP-DDGS), 40% dry distillers grains plus solubles (DDGS), 40% wet distillers grains plus solubles (WDGS), or 40% bran plus solubles (Bran). Eighteen Choice carcasses (3 head/pen) were randomly selected within each treatment (n = 88). Strip loins were aged for 2, 9, or 23 d, after which steaks were placed under …

Risk Of Nitrate Toxicity When Grazing Annual Forages, Mary E. Lenz

Department of Animal Science: Dissertations, Theses, and Student Research

Annual forages provide a valuable grazing resource for producers; however, annuals are prone to accumulating nitrate and toxicity can be a potential challenge. There are multiple publications regarding nitrate toxicity, but few, if any, address grazing high nitrate forages. There is variability on what amount of nitrate is considered toxic to cattle, and information is not available on the frequency producers experience toxicity when feeding annual forages. To understand the incidence of nitrate toxicity in the North Central Region of the U.S., a survey was distributed through the “UNL BeefWatch” newsletter to producers. Though producers appeared concerned about nitrates in …

Quantitative Microbial Risk Assessment For Parts, Ground, And Msc Poultry Product Including Intervention Analysis And Exploration Of Enterobacteriaceae As An Indicator Organism In Poultry Processing, Leigh Ann Parette

Graduate Theses and Dissertations

Samples collected at five different large bird poultry processing facilities over a period of 7 months from prescald to post debone locations were enumerated for Enterobacteriaceae, Salmonella spp., and Campylobacter spp. and the results were used to create Quantitative Microbial Risk Analyses (QMRA) models for parts, ground, and mechanically separated chicken (MSC) products. Sensitivity analyses indicated the points in the process at which reductions would be most advantageous to the endpoint and simulation models were run to test reductions required to meet the current USDA performance standards.

These data were analyzed to determine the reductions from one node (location) to …

Responsiveness Of Cobb Mv × 700 Broilers To Dietary Amino Acid And Energy Density, Craig William Maynard

Graduate Theses and Dissertations

As new broiler crosses are introduced to the industry, it is of paramount importance to understand their nutritional digressions from previous and other modern broilers. In particular, amino acids (AA) and dietary energy have been identified as key drivers of live performance and carcass composition. In addition, increased nutrient density in broiler diets have been linked to an increase in breast muscle myopathies such as white striping and woody breast. Therefore, a series of trials were conducted to determine the responsiveness of live performance, carcass characteristics, and breast muscle myopathies of Cobb MV × 700 broilers to dietary AA and …

The Evaluation Of Genetic And Phenotypic Differences Associated With Short Term Selection Of Four Different Feed Efficiency Strategies In Japanese Quail, Alex David Gilley

Graduate Theses and Dissertations

The concept of feed efficiency (FE), one unit of feed required to improve one unit of desired output, is relatively simple. However multiple outputs are desired in a commercial poultry breeding program, resulting in the need for nutrients to be partitioned in a particular manner. At the broadest level, nutrients are partitioned between production and maintenance requirements. The requirements for production and maintenance varies between birds. It is the poultry breeder’s responsibility to select individuals most aligned with the breeding objective. Irrespective of the breeding objective, various related and non-related traits influence a bird’s FE. To effectively improve FE, the …

Microbiota Metabolic Product Deoxycholic Acid Prevents Campylobacter Jejuni Chicken Colonization Through Modulating Ceca Anaerobes, Bilal Ali Alrubaye

Graduate Theses and Dissertations

Campylobacter jejuni is a prevalent infectious enteritis mainly foodborne from chickens. Despite of reducing C. jejuni food contamination dramatically decreases campylobacteriosis, few effective approaches are available for the bacterial reduction in chickens. The aim of this study was to use microbial metabolic product deoxycholic acid (DCA) to reduce C. jejuni chicken colonization. Broiler chicks were fed 0 or 1.5 g/kg DCA, lithocholic acid (LCA), or urodeoxycholic acid (UDCA) in diets or orally gavaged with cholic acid (CA, 1.5g/kg body weight). Birds were also transplanted with DCA modulated anaerobes (DCA-Anearo) or aerobes (DCA-Aero). Birds were infected with 109 CFU/bird human clinical …

Surveillance And Evaluation Of Manure Treatment Practices For Mitigation Of The Porcine Epidemic Diarrhea Virus (Pedv) In A Commercial Swine Farm Setting, Erin Boyles

Department of Animal Science: Dissertations, Theses, and Student Research

The emergence of the porcine epidemic diarrhea virus (PEDv) in the United States in 2013 resulted in billions of dollars in annual losses for the U.S. swine industry. Infection with PEDv causes severe diarrhea and vomiting in pigs, spreads rapidly through ingestion of infected manure, and produces nearly 100% mortality in pre-weaned piglets. Because swine manure slurry is a valuable crop nutrient source, concerns about virus persistence in stored manure remain a major barrier to proper manure management. Proper manure handling and application practices are necessary to control the risk of pathogen re-infection at affected production sites or infecting new …

Evaluation Of Genetic And Environmental Influences On Broiler Meat Quality, Sara Katherine Orlowski

Graduate Theses and Dissertations

The effect of both genetic and environmental influences on broiler meat quality were evaluated over three experiments. Selection response was assessed for broiler lines selected for high (HBY4) and low (LBY4) four day percentage breast yield which were formed from a random bred control (RAN). A modern random bred population (MRB) created in 2015 was evaluated for differences in 4 day percentage breast yield and the resulting differences in the incidence and severity of the woody breast and white striping myopathies. Additionally, the use of embryonic thermal manipulation to impact cell division and early caloric feed restriction to slow growth …

Effect Of Cooking Method On Meat Texture In Normal And Woody Broiler Breast Fillets Using Instrumental Analysis And Descriptive Sensory Analysis, Lynda D. Combs

Graduate Theses and Dissertations

Over the past few decades, broiler breast meat has changed in terms of meat quality. Meat quality has deteriorated with the rise in the occurrence of muscle myopathies. Conditions such as woody breast, which is described as pale and bulging areas of distinct hardness, compromise both the textural and technological traits that have been associated with breast meat. The Meullenet-Owens Razor Shear (MORS) method is a common method for indirectly assessing poultry meat tenderness. A blunt version of the MORS (BMORS) has been shown to be a more sensitive method at higher degrees of toughness. A slightly larger stainless steel …

Bats Of Saint Lucia, Lesser Antilles, Scott C. Pedersen, Gary G. Kwiecinski, Hugh H. Genoways, Roxanne J. Larsen, Peter A. Larsen, Carleton J. Phillips, Robert J. Baker

University of Nebraska State Museum: Mammalogy Papers

Eight species of bat have been previously recorded from the island of Saint Lucia: Noctilio leporinus, Monophyllus plethodon, Artibeus jamaicensis, Brachyphylla cavernarum, Ardops nichollsi, Sturnira paulsoni, Molossus molossus, and Tadarida brasiliensis. Herein, we add a ninth species to the fauna—Pteronotus davyi. These nine species represent nine genera from four families: Noctilionidae, Mormoopidae, Phyllostomidae, and Molossidae. This fauna includes four trophic guilds: N. leporinus (piscivore/insectivore), M. plethodon (nectarivore/pollenivore), A. jamaicensis × schwartzi, B. cavernarum, A. nichollsi, and S. paulsoni (frugivores), and P. davyi, M. molossus, and …

Multi-Metric Conservation Assessment For The Imperiled Clinch Dace, Michael James Moore, Donald J. Orth, Eric M. Hallerman

Southeastern Fishes Council Proceedings

Planning frameworks allow managers to spatially prioritize actions to promote species conservation. Traditional aquatic conservation planning frameworks are often organized at the ecological community or ecosystem level, which often neglect imperiled taxa occupying species-poor assemblages. In this study, we develop a multi-metric conservation assessment for the 15 geographically distinct candidate conservation areas (CCAs) occupied by the imperiled Clinch Dace (Chrosomus sp. cf. saylori). Clinch Dace habitat is threatened by anthropogenic landscape alterations, especially for coal mining and timber harvest. Our framework used four metrics to assess the conservation value of each subpopulation of Clinch Dace namely: “habitat …

Profitability Of Developing Beef Heifers On Stockpiled Winter Forages, Zachary David Mcfarlane, Chris Boyer, J. Travis Mulliniks

Journal of Applied Farm Economics

We estimate the profitability of developing heifers on one stockpiled cool-season grass and two stockpiled warm-season grasses during the winter months by comparing distributions of net present value (NPV) over an 11-year useful life. Furthermore, distributions of payback period and the break-even price for each calf over the heifer’s production life were generated for each forage species. These results are compared across forages as well as to a simulated drylot system for heifer development. Data comes from a grazing experiment in Tennessee, where heifers grazed big bluestem and Indian grass combination (BBIG), switchgrass (SW), or endophyte-infected tall fescue (TF) pastures. …

Automatic Identification Of Animals In The Wild: A Comparative Study Between C-Capsule Networks And Deep Convolutional Neural Networks., Joel Kamdem Teto, Ying Xie

Master of Science in Computer Science Theses

The evolution of machine learning and computer vision in technology has driven a lot of

improvements and innovation into several domains. We see it being applied for credit decisions, insurance quotes, malware detection, fraud detection, email composition, and any other area having enough information to allow the machine to learn patterns. Over the years the number of sensors, cameras, and cognitive pieces of equipment placed in the wilderness has been growing exponentially. However, the resources (human) to leverage these data into something meaningful are not improving at the same rate. For instance, a team of scientist volunteers took 8.4 years, …

Cryptic Diversity In The Mexican Highlands: Thousands Of Uce Loci Help Illuminate Phylogenetic Relationships, Species Limits And Divergence Times Of Montane Rattlesnakes (Viperidae: Crotalus ), Christopher Blair, Robert W. Bryson Jr, Charles W. Linkem, David Lazcano, John Klicka, John E. Mccormack

Publications and Research

With the continued adoption of genome‐scale data in evolutionary biology comes the challenge of adequately harnessing the information to make accurate phylogenetic inferences. Coalescent‐based methods of species tree inference have become common, and concatenation has been shown in simulation to perform well, particularly when levels of incomplete lineage sorting are low. However, simulation conditions are often overly simplistic, leaving empiricists with uncertainty regarding analytical tools. We use a large ultraconserved element data set (>3,000 loci) from rattlesnakes of the Crotalus triseriatus group to delimit lineages and estimate species trees using concatenation and several coalescent‐based methods. Unpartitioned and partitioned maximum …

The Relative Effects Of Forest Amount, Forest Configuration, And Urban Matrix Quality On Forest Breeding Birds, Alexandra V. Shoffner, Andrew M. Wilson, Wenwu Tang, Sara A. Gagné

Environmental Studies Faculty Publications

Urbanization modifies landscape structure in three major ways that impact avian diversity in remnant habitat: habitat amount is reduced and habitat configuration and matrix quality are altered. The relative effects of these three components of landscape structure are relatively well-studied in agricultural landscapes, but little is known about the relative effect of urban matrix quality. We addressed this gap by investigating the relative effects of forest amount, forest configuration, and matrix quality, indicated by degree of urbanization and agriculture amount, on the diversity of three guilds of forest birds using data from 13,763 point counts from Pennsylvania, USA. Forest amount …

Pathogenesis Of Rift Valley Fever Virus Aerosol Infection In Stat2 Knockout Hamsters, Brady T. Hickerson, Jonna B. Westover, Arnaud J. Van Wettere, Johanna D. Rigas, Jinxin Miao, Bettina L. Conrad, Neil E. Motter, Zhongde Wang, Brian B. Gowen

Animal, Dairy, and Veterinary Science Faculty Publications

Rift Valley fever virus (RVFV) is an emerging pathogen capable of causing severe disease in livestock and humans and can be transmitted by multiple routes including aerosol exposure. Several animal models have been developed to gain insight into the pathogenesis associated with aerosolized RVFV infection, but work with these models is restricted to high containment biosafety level (BSL) laboratories limiting their use for antiviral and vaccine development studies. Here, we report on a new RVFV inhalation infection model in STAT2 KO hamsters exposed to aerosolized MP-12 vaccine virus by nose-only inhalation that enables a more accurate delivery and measurement of …

Courtship Behavior, Communication, And Copulation In Tigrosa Annexa, Samuel White

Scholars Week

The evolution of multimodal communication, where signalers use multiple signal components in multiple sensory modalities, has become the subject of investigation by many researchers. Signaling puts males at risk of predation, so why do males of some species evolve extra signals that may increase this risk? In some wolf spider species, males incorporate many visual and vibrational signals into a display that they use to attract a female for mating. Female spiders are often aggressive toward courting males and so the male display also functions to decrease the odds of cannibalism. Female wandering spiders deposit silk containing pheromones that communicate …

Improved Reference Genome For The Domestic Horse Increases Assembly Contiguity And Composition, Theodore S. Kalbfleisch, Edward S. Rice, Michael S. Depriest Jr., Brian P. Walenz, Matthew S. Hestand, Joris R. Vermeesch, Brendan L. O'Connell, Ian T. Fiddes, Alisa O. Vershinina, Nedda F. Saremi, Jessica L. Petersen, Carrie J. Finno, Rebecca R. Bellone, Molly E Mccue, Samantha A. Brooks, Ernest Bailey, Ludovic Orlando, Richard E. Green, Donald C. Miller, Douglas F. Antczak, James N. Macleod

Maxwell H. Gluck Equine Research Center Faculty Publications

Recent advances in genomic sequencing technology and computational assembly methods have allowed scientists to improve reference genome assemblies in terms of contiguity and composition. EquCab2, a reference genome for the domestic horse, was released in 2007. Although of equal or better quality compared to other first-generation Sanger assemblies, it had many of the shortcomings common to them. In 2014, the equine genomics research community began a project to improve the reference sequence for the horse, building upon the solid foundation of EquCab2 and incorporating new short-read data, long-read data, and proximity ligation data. Here, we present EquCab3. The count of …

Does Experience With Sagebrush In Utero And Early In Life Influence Use Of Sagebrush By Sheep?, Juan J. Villalba, Fred Provenza, Ashley Longmore

Poisonous Plant Research (PPR)

Learning from mother begins early in the developmental process and can have lifelong effects when it comes to foraging behavior. Pregnancy is not just an incubation period but a starting point for animal well-being and disease later in life. A better understanding of the effects that early exposure to unpalatable feeds impinges on their use later in life may help create management plans that utilize grazing animals to their full potential as landscape manipulators.

Thus, the objective of this research was to explore how experience in utero and early in life with sagebrush (Artemesia tridentata spp. tridentata) -a …